FastQCFastQC Report
Thu 26 May 2016
SRR1512856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922878
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT104170.5417400375894882No Hit
TATCAACGCAGAGTACTTTTTTTTT62110.32300541167978414No Hit
GTCCTACAGTGGACATTTCTAAATT59280.30828788929926915No Hit
GTCCTAAAGTGTGTATTTCTCATTT58280.3030873513556242No Hit
GGTATCAACGCAGAGTACTTTTTTT58200.3026713083201326No Hit
CTGTAGGACGTGGAATATGGCAAGA52600.27354829583572127No Hit
CTTTAGGACGTGAAATATGGCGAGG49390.2568545690366211No Hit
GTCCTACAGTGTGCATTTCTCATTT31080.16163271928848322No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27740.14426292255670928No Hit
CTGAAGGACCTGGAATATGGCGAGA25020.13011745934999516No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23950.12455288375029513No Hit
CTGTAGGACCTGGAATATGGCGAGA22960.1194043511860867No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20210.10510287184106325No Hit
GTCCTTCAGTGTGCATTTCTCATTT19850.10323067818135108No Hit
ATTTAGAAATGTCCACTGTAGGACG19730.1026066136281137No Hit
TTTCTAAATTTTCCACCTTTTTCAG19290.10031837693290994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.013.8443981
TAGGACC9250.013.3497254
GCCGGTT752.0776562E-411.39801611
GTCCTAA14600.011.2819071
AGGACCT17200.011.2108425
TAGAAAT8450.011.128824
AAATGTC7900.011.0619427
GTACTAG700.001464262110.8813111
ACTGGTC1408.36917E-910.855828
ACAGTCC1502.1373125E-910.7653538
TGTAGGA31450.010.7522662
GTATCAA41650.010.6298511
GGCGAGG12600.010.62937919
AATGTCC8850.010.6258238
GACGTGG15150.010.5960677
GAAATGT8500.010.50460156
TCCTACA31000.010.4487262
AGGACGT31900.010.3623725
GTCCTAC28950.010.35979751
CACCTTT7250.010.35073214
Produced by FastQC (version 0.11.5)