##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512856_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1922878
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.875170967684898	31.0	30.0	34.0	22.0	34.0
2	28.948604123610547	31.0	30.0	34.0	20.0	34.0
3	29.067961149901347	31.0	30.0	34.0	22.0	34.0
4	32.47869495620627	35.0	33.0	37.0	24.0	37.0
5	32.681205463893185	35.0	33.0	37.0	25.0	37.0
6	32.62528459943896	36.0	33.0	37.0	25.0	37.0
7	32.517239263229385	36.0	33.0	37.0	23.0	37.0
8	32.43542336019238	36.0	33.0	37.0	23.0	37.0
9	33.710135016366095	38.0	34.0	39.0	22.0	39.0
10	33.56859821579944	38.0	34.0	39.0	19.0	39.0
11	33.63133958576675	38.0	34.0	39.0	19.0	39.0
12	33.53544374630112	38.0	34.0	39.0	18.0	39.0
13	33.49576988243664	38.0	34.0	39.0	17.0	39.0
14	34.32969850401326	39.0	34.0	41.0	17.0	41.0
15	34.52489081470587	39.0	34.0	41.0	17.0	41.0
16	34.46281615370294	39.0	34.0	41.0	17.0	41.0
17	34.242606655232414	39.0	34.0	41.0	17.0	41.0
18	34.24799389248824	39.0	34.0	41.0	17.0	41.0
19	34.37602437596145	39.0	34.0	41.0	18.0	41.0
20	34.372386599669866	39.0	34.0	41.0	18.0	41.0
21	34.2655217855735	39.0	34.0	41.0	16.0	41.0
22	34.27602531205828	39.0	34.0	41.0	15.0	41.0
23	33.948441866826705	39.0	34.0	41.0	10.0	41.0
24	34.02596888622159	39.0	34.0	41.0	2.0	41.0
25	33.75137060177505	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7097860062595522
1101	2	0.34264359171795533
1101	3	0.6946829436931132
1101	4	0.7753959819133485
1101	5	0.7099147845082356
1101	6	0.7442887398975984
1101	7	0.3272208866946187
1101	8	0.6968916341322284
1101	9	0.7961412439376119
1101	10	0.9132978900238982
1101	11	0.8128427178308257
1101	12	0.6887692271929282
1101	13	1.0183284600359954
1101	14	1.2781047636751595
1101	15	0.9997340191090771
1101	16	0.955806256567648
1101	17	0.3657584229322879
1101	18	0.7860364587741486
1101	19	0.3390385664095419
1101	20	-0.11100191148161542
1101	21	0.7729446560963567
1101	22	0.9106736869894689
1101	23	0.37880597940681326
1101	24	0.9768553539430229
1101	25	0.6683281319444063
1102	1	1.1060390529828616
1102	2	1.1087860613195772
1102	3	0.9300839572969437
1102	4	1.1737007145495753
1102	5	1.0535735401013113
1102	6	0.9357509344120913
1102	7	0.9354897875379962
1102	8	0.9171430787289978
1102	9	1.3011584546894284
1102	10	1.3192720793568924
1102	11	1.0605755710643479
1102	12	1.2015150128261212
1102	13	1.2621220003415416
1102	14	1.4041236026699906
1102	15	1.4364576180912039
1102	16	1.2564674542878222
1102	17	1.5584237556014742
1102	18	1.427925206423076
1102	19	1.2937407413414377
1102	20	1.412239607433115
1102	21	1.4908176028078373
1102	22	1.3563967831108883
1102	23	1.2781848111080265
1102	24	0.6164217541109309
1102	25	1.0076587953833922
1103	1	0.6806949089105068
1103	2	0.7058436544028268
1103	3	0.8885055921475846
1103	4	0.9180080287889467
1103	5	0.743165284261508
1103	6	0.3846594761370099
1103	7	0.2568785731937737
1103	8	0.5138711654695385
1103	9	0.7134419886003016
1103	10	0.7782196878483987
1103	11	0.5848813007422891
1103	12	0.7037473667768381
1103	13	0.7279671451835057
1103	14	0.894207499701885
1103	15	0.7775096203288214
1103	16	0.7905726201641556
1103	17	0.576903130771349
1103	18	0.8645690824251915
1103	19	0.7379806659236436
1103	20	0.8957549020001139
1103	21	0.6114086808568757
1103	22	0.8144449469087434
1103	23	0.19589558239329818
1103	24	0.25069169605690433
1103	25	0.6350615412085219
1104	1	0.9259217370763189
1104	2	0.9452196052703918
1104	3	0.9803495651972831
1104	4	1.1994748282603922
1104	5	0.9186489350062672
1104	6	0.89611722194919
1104	7	1.067371084257502
1104	8	0.8668438724869887
1104	9	1.1849889642914277
1104	10	1.2207736197899521
1104	11	0.8854107864704446
1104	12	1.1334608364617509
1104	13	1.1348064580654338
1104	14	1.3595875867183551
1104	15	1.2866052811059419
1104	16	1.2445542968378476
1104	17	1.4869618928867823
1104	18	1.3566947989509828
1104	19	0.825258474591422
1104	20	0.9375030716008794
1104	21	1.022468145664277
1104	22	0.9883038724967292
1104	23	1.2405965249605657
1104	24	1.0108037267566772
1104	25	0.6877007695540769
1105	1	0.6024159002008496
1105	2	0.6911320814230777
1105	3	0.7505933787147221
1105	4	0.820139453550695
1105	5	0.7035792077228322
1105	6	0.8671579093047015
1105	7	0.7725429001019322
1105	8	0.8844584761592316
1105	9	0.5820195579555261
1105	10	0.6195570200175169
1105	11	0.72073515275207
1105	12	0.9970933413332119
1105	13	0.9438706429659618
1105	14	1.0097245010812301
1105	15	0.9167532249555919
1105	16	0.9463024310053711
1105	17	1.227080927109121
1105	18	1.0456366197244549
1105	19	0.6986520094432151
1105	20	0.8842782299852345
1105	21	0.7911644748062727
1105	22	0.8262010179260386
1105	23	1.0888338142338654
1105	24	1.0376064983429814
1105	25	1.2635854153371042
1106	1	0.7385399548949145
1106	2	0.8351559655164742
1106	3	0.7160722064080467
1106	4	0.8538267196113409
1106	5	0.799911293379644
1106	6	0.8332796723480129
1106	7	0.7216109621292759
1106	8	0.785923859501473
1106	9	0.961395974323004
1106	10	0.6920814006352458
1106	11	0.5189234755183421
1106	12	0.7263729991133729
1106	13	0.7450317460138578
1106	14	1.086934034348367
1106	15	0.8732500548387137
1106	16	0.9423747962435698
1106	17	1.0429022292760024
1106	18	1.074549613384221
1106	19	0.9489448314838071
1106	20	1.0688325039138888
1106	21	1.0332073541381348
1106	22	0.9433743940368586
1106	23	1.2060326531308192
1106	24	0.8601690731399145
1106	25	1.0073852269124899
1107	1	0.5770744833741475
1107	2	0.5505487200757244
1107	3	0.6032238628613626
1107	4	0.6665994476001273
1107	5	0.5298797343863768
1107	6	0.6916120867422748
1107	7	0.3384895098904792
1107	8	0.7078626108995749
1107	9	0.6591936711991337
1107	10	0.7094060276772538
1107	11	0.5398621586523973
1107	12	0.6233257971515656
1107	13	0.5556961652181087
1107	14	0.8953851231598833
1107	15	0.6053123641588556
1107	16	0.6938359093675999
1107	17	1.0812521822788383
1107	18	1.034532442351285
1107	19	0.6694482000518889
1107	20	0.909802220933166
1107	21	0.6184783361426796
1107	22	0.5600678412136091
1107	23	0.9855857336771408
1107	24	0.6986655312861814
1107	25	1.0127478927809932
1108	1	0.9960668792949043
1108	2	0.8787562936369255
1108	3	0.8361312151667448
1108	4	1.0430662210799468
1108	5	0.8813420573181503
1108	6	0.9324554020190945
1108	7	0.8289844108339963
1108	8	0.8276255874261125
1108	9	0.997737960510193
1108	10	1.0240890214973533
1108	11	0.7570476320635535
1108	12	0.7921121962767188
1108	13	0.8980615679291333
1108	14	1.0539962978502544
1108	15	0.9344736265886056
1108	16	0.767997298955855
1108	17	0.941404518180768
1108	18	1.0747030890794917
1108	19	1.008365063814253
1108	20	1.0394339698713893
1108	21	1.194769630870688
1108	22	1.1909253982442038
1108	23	1.3501873460471217
1108	24	1.2619790278910443
1108	25	1.3682449554192786
1109	1	0.4488600320307974
1109	2	0.40614344144434966
1109	3	0.43946917159093246
1109	4	0.4875757705194488
1109	5	0.30709352165036563
1109	6	0.49095722368655004
1109	7	0.30203900147838425
1109	8	0.35231628568248397
1109	9	0.3518160356078468
1109	10	0.371149753642257
1109	11	0.356312178894612
1109	12	0.13023382173849285
1109	13	0.2828386628467001
1109	14	0.4292470687188441
1109	15	0.21601519411329662
1109	16	-0.1993358986537075
1109	17	0.4623647214271145
1109	18	0.42989049391380263
1109	19	0.30702351919294557
1109	20	0.2399361478137365
1109	21	0.12227596111206651
1109	22	0.24910699664605573
1109	23	0.5059643009995511
1109	24	0.36913760839008347
1109	25	-0.0867053569574594
1110	1	0.5853600731778137
1110	2	0.5998962565598163
1110	3	0.5610133719662187
1110	4	0.40462213095599964
1110	5	0.43475334116050135
1110	6	0.49691610724342894
1110	7	0.46871006553601546
1110	8	0.4511518356149651
1110	9	0.5123831022067549
1110	10	0.5468677167121285
1110	11	0.66014540553887
1110	12	0.5219450329286417
1110	13	0.6523728891016773
1110	14	0.5382638351329945
1110	15	0.41738209168487117
1110	16	0.680982196044738
1110	17	0.6819568152532014
1110	18	0.011395507253602943
1110	19	0.48840479832696815
1110	20	0.5811330631905491
1110	21	0.7866673967534226
1110	22	0.744920994546618
1110	23	0.855688804455589
1110	24	0.7262673182313861
1110	25	0.8649137100460038
1111	1	0.32128985983150216
1111	2	0.3420015180618705
1111	3	0.39119206086782654
1111	4	0.3886896638272148
1111	5	0.3544832843430257
1111	6	0.3104083143074021
1111	7	0.24905446463820624
1111	8	0.41917940969166523
1111	9	0.48770112809766886
1111	10	0.4538745268597211
1111	11	0.182386165266891
1111	12	-0.08612598665759919
1111	13	0.15081677682668726
1111	14	0.30886214842621484
1111	15	0.19473434229293218
1111	16	0.27519948001244643
1111	17	-0.13972248142201948
1111	18	0.1559907791094659
1111	19	0.20699287589673077
1111	20	0.37312840528828417
1111	21	0.3061497630340497
1111	22	-0.015601521916124739
1111	23	0.3181575912354333
1111	24	0.35056098000890756
1111	25	0.3787530260508447
1112	1	0.056309797989715804
1112	2	0.005694450307803578
1112	3	0.04907547817294855
1112	4	0.2695906151387817
1112	5	0.23111703218791035
1112	6	-0.04627649065288608
1112	7	-0.204742247240425
1112	8	0.019555130994227454
1112	9	-0.11092540272917262
1112	10	-0.06001135764601173
1112	11	-0.03527315074861548
1112	12	-0.07809835550408906
1112	13	-0.0394951054528363
1112	14	-0.06506889895810275
1112	15	-0.22003959754810154
1112	16	-5.202463307085736E-4
1112	17	0.3152275679282184
1112	18	-0.038325845106392364
1112	19	0.0500549837728812
1112	20	-0.22255868083705366
1112	21	-0.08305548768981907
1112	22	-0.22725408797274582
1112	23	-0.09104751845814008
1112	24	-0.12165148876999154
1112	25	-0.05717141406406512
1113	1	-0.18172246653192303
1113	2	-0.12314568642747048
1113	3	-0.2061557881301468
1113	4	-0.20029371876619706
1113	5	-0.30717279798484043
1113	6	-0.4897716402235588
1113	7	-0.39065428043405603
1113	8	-0.32937019023598424
1113	9	-0.2982218983596141
1113	10	-0.37346722814114486
1113	11	-0.49490254820521073
1113	12	-0.5760207206068486
1113	13	-0.44631057620875936
1113	14	-0.1554239500915955
1113	15	-0.3736876320151339
1113	16	-0.48838826411893166
1113	17	-0.3432919169321167
1113	18	-0.4888556284094676
1113	19	-0.8546036831732309
1113	20	-0.7582408522698572
1113	21	-0.5171753437760032
1113	22	-1.115216271796477
1113	23	-4.564131970666782
1113	24	-2.0948393730189743
1113	25	-1.278139065248972
1114	1	-0.6148087780689018
1114	2	-0.8381489306870868
1114	3	-0.6539176492455958
1114	4	-0.5997052158530352
1114	5	-0.6426587053568937
1114	6	-1.1996510000170666
1114	7	-1.0143435128275549
1114	8	-0.8752366610463227
1114	9	-1.1287898283529785
1114	10	-0.812777448292735
1114	11	-1.0044629966112524
1114	12	-1.9079645146428383
1114	13	-1.0913133045888515
1114	14	-2.9792244078902037
1114	15	-1.8059304138154388
1114	16	-1.7187742798701535
1114	17	-1.5822344172667684
1114	18	-1.4361056125105947
1114	19	-1.554946276099372
1114	20	-1.075900675972477
1114	21	-1.0481122165765697
1114	22	-1.2692842487081961
1114	23	-5.2315585590087075
1114	24	-2.3349653943826105
1114	25	-2.381422482790743
1115	1	-1.5088101425412503
1115	2	-1.2172499119506952
1115	3	-0.9721711873932222
1115	4	-1.0657767412784338
1115	5	-0.9066490294370055
1115	6	-1.2008296728301353
1115	7	-1.6201174631498247
1115	8	-1.90169225137112
1115	9	-3.061694791778038
1115	10	-3.791061875333135
1115	11	-2.5590739404504887
1115	12	-2.8430501012157876
1115	13	-2.079415970823405
1115	14	-2.7017963396540345
1115	15	-2.4213462170409557
1115	16	-2.6550349532578785
1115	17	-2.38925257452086
1115	18	-5.250216426133264
1115	19	-3.137223981185972
1115	20	-3.4197406481092685
1115	21	-2.938980539167595
1115	22	-2.385048678205006
1115	23	-2.7440171015065964
1115	24	-2.5587656426233423
1115	25	-2.7324371410070043
1116	1	-1.3712094310846332
1116	2	-1.1783283334170775
1116	3	-1.6227419364076958
1116	4	-1.423568545624999
1116	5	-1.4624232723856814
1116	6	-3.767718275550269
1116	7	-2.196989246886506
1116	8	-2.689176476855856
1116	9	-3.8237137004144834
1116	10	-2.92053902494899
1116	11	-2.457775216815694
1116	12	-2.3339429835681784
1116	13	-3.7246887758815674
1116	14	-6.692597495126083
1116	15	-4.631277156732679
1116	16	-3.2837947700047714
1116	17	-4.136525670525224
1116	18	-2.935290814483377
1116	19	-2.7451815030409783
1116	20	-3.4534771497463765
1116	21	-4.230407945582886
1116	22	-3.7452938182927333
1116	23	-2.9210403062574777
1116	24	-3.7103020320383493
1116	25	-3.581204238575026
1201	1	0.4988682541591096
1201	2	0.4619168784884877
1201	3	0.7989987509185568
1201	4	0.8186525864162704
1201	5	0.2504499093599648
1201	6	0.7737124588836224
1201	7	0.8830417740154175
1201	8	0.8452129856915036
1201	9	1.2978167658743018
1201	10	1.11968126384388
1201	11	1.2313210198927962
1201	12	1.0315340231364303
1201	13	1.185101869971838
1201	14	1.2590861448698973
1201	15	1.2404530392437678
1201	16	1.20204166147834
1201	17	1.402633169495978
1201	18	1.1716305703353953
1201	19	1.4441511584519304
1201	20	1.4537342494746
1201	21	1.5943355335260279
1201	22	1.3532497557801335
1201	23	1.7041480947425995
1201	24	1.598265558852205
1201	25	1.752835167525916
1202	1	0.9699456261959618
1202	2	0.9212061471415467
1202	3	1.010393103830257
1202	4	1.1215314002550656
1202	5	0.9316688547037657
1202	6	1.2422029792108944
1202	7	1.1361739533176518
1202	8	1.168982360020209
1202	9	1.4224506041883487
1202	10	1.3538050458381221
1202	11	1.3352761648559834
1202	12	1.5483776909803453
1202	13	1.4090937969126784
1202	14	1.8459728585894908
1202	15	1.5910208431763877
1202	16	1.3508348017730754
1202	17	1.760122120164091
1202	18	1.6018385409613956
1202	19	1.6046914104448575
1202	20	1.4479307018975334
1202	21	1.6000096579159901
1202	22	1.582034255580247
1202	23	1.6910708258758333
1202	24	1.6796936999667764
1202	25	1.9825113516329367
1203	1	1.237892974115777
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2211	12	-0.3312133566782194
2211	13	-0.30844611680650047
2211	14	-0.09679044455688768
2211	15	-0.5364167968832092
2211	16	-0.5569840560765655
2211	17	-0.1243840618571852
2211	18	-0.5923631279586772
2211	19	-0.3433260938501732
2211	20	-0.4347671980450798
2211	21	-0.21261603302986032
2211	22	-0.21967515240025648
2211	23	-0.04471844044812201
2211	24	-0.47200489122740663
2211	25	-0.053700869934871776
2212	1	0.07297519613846148
2212	2	0.20424691802123718
2212	3	-0.16907998246215072
2212	4	0.16626669392963578
2212	5	-0.12838819563930315
2212	6	-0.18476163770198895
2212	7	-0.06303325250045333
2212	8	0.14800009808664782
2212	9	0.1354695669142174
2212	10	-0.09239390194725416
2212	11	-0.25411444744715084
2212	12	0.12091705155216204
2212	13	0.16836026844909213
2212	14	0.2632751164113216
2212	15	-0.16107756024698716
2212	16	-0.1367958721352096
2212	17	-0.045084144562615336
2212	18	0.3094001483313278
2212	19	0.1924213774712129
2212	20	0.22387961357229358
2212	21	0.3046737760149867
2212	22	0.33297047315490147
2212	23	0.32598806388777035
2212	24	0.3727543471930801
2212	25	0.31247310539486506
2213	1	-0.34149547020630067
2213	2	-0.21565375108271567
2213	3	-0.3646126788821
2213	4	-0.18554575002428209
2213	5	-0.32926380783493414
2213	6	-0.07588868122823555
2213	7	-0.27318492181134246
2213	8	-0.08702654535682797
2213	9	-0.5672646194691708
2213	10	-0.21629410543763328
2213	11	-0.2819874556557451
2213	12	-0.3176637755155127
2213	13	-0.3287874172789884
2213	14	-0.4006756147744426
2213	15	-0.3745907008967748
2213	16	-0.3797237192759937
2213	17	-0.22896011890212975
2213	18	-0.25761975233777434
2213	19	-0.3416843857901455
2213	20	-0.2101317336029851
2213	21	-0.3343196364151737
2213	22	-0.4094680486082183
2213	23	-0.1868654575304518
2213	24	-0.008673706887073251
2213	25	0.19717942394352406
2214	1	-2.395146464693191
2214	2	-1.0856188288157824
2214	3	-1.301723127198919
2214	4	-3.053400866934105
2214	5	-1.453804566729474
2214	6	-1.1384827453292097
2214	7	-0.8664875356632535
2214	8	-0.7862909840301349
2214	9	-0.6930891483731045
2214	10	-0.6895786820273031
2214	11	-0.5409090312349747
2214	12	-0.4707444025208787
2214	13	-0.8235366683115899
2214	14	-0.8079342832965395
2214	15	-1.1441985787929312
2214	16	-1.011095703463475
2214	17	-3.022534067245541
2214	18	-2.7706621997327865
2214	19	-1.6015487074649997
2214	20	-1.4811766096023433
2214	21	-1.2878887541404396
2214	22	-2.3829857357614337
2214	23	-1.5035056075012037
2214	24	-2.454851092503354
2214	25	-2.4327245398224058
2215	1	-2.029604181670276
2215	2	-3.5686025566631017
2215	3	-2.072108910720438
2215	4	-3.8012375144002597
2215	5	-2.3771791600266
2215	6	-2.1087344538766395
2215	7	-2.295472831270306
2215	8	-1.7558895902067135
2215	9	-3.966575177332359
2215	10	-2.4357775824868995
2215	11	-2.096798521782599
2215	12	-2.01811058747548
2215	13	-2.012684222228426
2215	14	-1.6781473754698908
2215	15	-1.956613064498086
2215	16	-2.0221475235419035
2215	17	-2.2840997161469936
2215	18	-2.1546159578866124
2215	19	-2.018101280299149
2215	20	-2.035869811314747
2215	21	-2.5103850184016103
2215	22	-2.4400798709963567
2215	23	-2.4549160472915688
2215	24	-2.446024733053889
2215	25	-3.4996910301637705
2216	1	-3.2429873716889794
2216	2	-3.384201288149683
2216	3	-4.633573925456481
2216	4	-5.04503032595008
2216	5	-4.849156559574094
2216	6	-3.976413503798682
2216	7	-5.6197816999717
2216	8	-3.4045960239598756
2216	9	-4.257650924015582
2216	10	-2.984271144999152
2216	11	-3.205747205497641
2216	12	-3.506252507860392
2216	13	-3.1935228540016354
2216	14	-4.1370078296574455
2216	15	-3.675999596968733
2216	16	-3.9697964478263437
2216	17	-3.644412300758642
2216	18	-3.153146243800073
2216	19	-4.001675338639561
2216	20	-4.4994762982857175
2216	21	-4.352820620183007
2216	22	-3.9255056601604643
2216	23	-3.968854600949747
2216	24	-4.29353158757009
2216	25	-4.392344327022673
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97178.0
3	1.0
4	848.0
5	5415.0
6	6775.0
7	6089.0
8	5465.0
9	5003.0
10	4603.0
11	4249.0
12	3916.0
13	3710.0
14	3742.0
15	3730.0
16	3922.0
17	4157.0
18	4710.0
19	5711.0
20	7418.0
21	9811.0
22	11992.0
23	14697.0
24	16856.0
25	19236.0
26	21242.0
27	23219.0
28	25965.0
29	30370.0
30	36795.0
31	45960.0
32	57120.0
33	72102.0
34	94090.0
35	123859.0
36	169123.0
37	265216.0
38	708583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.898608258611766	18.894746948521433	12.802173604501663	24.404471188365143
2	14.339225790613538	20.573843647734105	42.77385155334938	22.313079008302978
3	19.02916782172579	26.377112197892444	29.294778543309647	25.29894143707212
4	11.845815136067955	16.877492290704794	38.85703229712292	32.41966027610433
5	12.377928773233581	39.564340430489615	35.08530942741469	12.972421368862111
6	30.493277510367918	37.25745740410719	18.972035775553373	13.277229309971519
7	27.667983984002742	32.300766812971645	22.17272523341916	17.858523969606455
8	25.137476553629522	37.33672137616594	20.818867254628497	16.706934815576044
9	27.778358996299808	14.704950055979152	20.392546314875048	37.124144632846
10	16.164225120880072	28.838973535736205	33.83382289176256	21.162978451621164
11	33.50657475935751	22.606991111335507	25.560440784484367	18.325993344822614
12	24.335339253922477	25.035242428343867	31.705379545856804	18.924038771876855
13	29.369981486117712	22.814703468190945	25.850053688697162	21.96526135699418
14	20.868324593976453	23.323932929209647	27.979475263820312	27.828267212993584
15	22.50070137396427	32.78083976454655	23.58453885507662	21.133920006412563
16	20.964862329831675	28.328594940833423	29.468307597494466	21.238235131840437
17	20.677931514486758	29.544691433089998	28.453622952261913	21.323754100161327
18	20.51365077910747	27.136116311176544	31.920188076938295	20.430044832777696
19	23.20670427121919	26.563576888400263	28.63739716529137	21.59232167508917
20	23.800580404200772	27.1047720677225	29.307705321239407	19.786942206837317
21	22.806246860824917	26.532346840786246	27.89310279318269	22.76830350520615
22	23.310880281451844	28.708046292955842	27.91149135013486	20.069582075457458
23	21.485824404179386	28.421361673419927	29.220508805610272	20.872305116790418
24	22.95436267811829	26.66057648513241	29.856464311275943	20.528596525473354
25	22.008778130462257	27.734394926340535	29.517192414079574	20.739634529117637
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	362.0
1	362.0
2	820.0
3	1278.0
4	1278.0
5	1278.0
6	2623.5
7	3969.0
8	3969.0
9	3969.0
10	4702.5
11	5436.0
12	5436.0
13	5436.0
14	7412.0
15	9388.0
16	9388.0
17	9388.0
18	16899.0
19	24410.0
20	24410.0
21	24410.0
22	38698.0
23	52986.0
24	52986.0
25	52986.0
26	78440.5
27	103895.0
28	103895.0
29	103895.0
30	134595.0
31	165295.0
32	165295.0
33	165295.0
34	182191.5
35	199088.0
36	199088.0
37	199088.0
38	208886.5
39	218685.0
40	218685.0
41	218685.0
42	227786.0
43	236887.0
44	236887.0
45	236887.0
46	244365.0
47	251843.0
48	251843.0
49	251843.0
50	237547.5
51	223252.0
52	223252.0
53	223252.0
54	194184.5
55	165117.0
56	165117.0
57	165117.0
58	140337.5
59	115558.0
60	115558.0
61	115558.0
62	94734.0
63	73910.0
64	73910.0
65	73910.0
66	56613.0
67	39316.0
68	39316.0
69	39316.0
70	29417.5
71	19519.0
72	19519.0
73	19519.0
74	13830.0
75	8141.0
76	8141.0
77	8141.0
78	5626.0
79	3111.0
80	3111.0
81	3111.0
82	2063.5
83	1016.0
84	1016.0
85	1016.0
86	655.5
87	295.0
88	295.0
89	295.0
90	190.5
91	86.0
92	86.0
93	86.0
94	55.0
95	24.0
96	24.0
97	24.0
98	17.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3343945897763665
2	0.010089043610671087
3	0.009204952160251457
4	0.010349070507853332
5	0.011961237270383248
6	0.08144042419747899
7	0.08055633274705935
8	0.10427078577008005
9	0.08461275234310237
10	0.09147746242871363
11	0.08669296752056033
12	0.09870621017038003
13	0.08409269854873788
14	0.08757705897097995
15	0.08575687069070426
16	0.08799310200647155
17	0.09969431237967256
18	0.10203455445431275
19	0.0953778658864473
20	0.09376569912391738
21	0.08284456944226311
22	0.1030226566636053
23	0.0951698443687015
24	0.09116543015209493
25	0.0904893602194211
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1922878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.737280318226254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23008979698388	43.8182525381326
2	13.250835254379165	15.036327085566914
3	4.336481636500792	7.381205226149579
4	1.8911809299999232	4.292018502315579
5	0.9617649857345343	2.7283964797937577
6	0.5644376341811896	1.9214793763616764
7	0.36650443601651056	1.4556125446899526
8	0.2462448590933471	1.117701087784109
9	0.1833232001111082	0.936113381418441
>10	0.8667173849690519	8.611888268796228
>50	0.05694132097818051	2.2151677796194873
>100	0.03799258467007837	4.300662830531761
>500	0.004436134152466919	1.6935348240685921
>1k	0.0024953254607626416	2.4222943678313342
>5k	4.6209730754863735E-4	1.5231168849961416
>10k+	9.241946150972746E-5	0.5462288219439119
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10417	0.5417400375894882	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6211	0.32300541167978414	No Hit
GTCCTACAGTGGACATTTCTAAATT	5928	0.30828788929926915	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5828	0.3030873513556242	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5820	0.3026713083201326	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5260	0.27354829583572127	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4939	0.2568545690366211	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3108	0.16163271928848322	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2774	0.14426292255670928	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2502	0.13011745934999516	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2395	0.12455288375029513	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2296	0.1194043511860867	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2021	0.10510287184106325	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1985	0.10323067818135108	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1973	0.1026066136281137	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1929	0.10031837693290994	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.200537943644891E-5
2	0.0	0.0	0.0	0.0	5.200537943644891E-5
3	0.0	0.0	0.0	0.0	5.200537943644891E-5
4	0.0	0.0	0.0	0.0	5.200537943644891E-5
5	0.0	0.0	0.0	0.0	5.200537943644891E-5
6	0.0	0.0	0.0	0.0	5.200537943644891E-5
7	0.0	0.0	0.0	0.0	5.200537943644891E-5
8	0.0	0.0	0.0	0.0	5.200537943644891E-5
9	0.0	0.0	0.0	0.0	1.0401075887289782E-4
10	0.0	0.0	0.0	0.0	1.0401075887289782E-4
11	0.0	0.0	0.0	0.0	1.0401075887289782E-4
12	0.0	0.0	0.0	0.0	1.0401075887289782E-4
13	0.0	0.0	0.0	0.0	1.0401075887289782E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1905	0.0	13.844398	1
TAGGACC	925	0.0	13.349725	4
GCCGGTT	75	2.0776562E-4	11.398016	11
GTCCTAA	1460	0.0	11.281907	1
AGGACCT	1720	0.0	11.210842	5
TAGAAAT	845	0.0	11.12882	4
AAATGTC	790	0.0	11.061942	7
GTACTAG	70	0.0014642621	10.881311	1
ACTGGTC	140	8.36917E-9	10.85582	8
ACAGTCC	150	2.1373125E-9	10.765353	8
TGTAGGA	3145	0.0	10.752266	2
GTATCAA	4165	0.0	10.629851	1
GGCGAGG	1260	0.0	10.629379	19
AATGTCC	885	0.0	10.625823	8
GACGTGG	1515	0.0	10.596067	7
GAAATGT	850	0.0	10.5046015	6
TCCTACA	3100	0.0	10.448726	2
AGGACGT	3190	0.0	10.362372	5
GTCCTAC	2895	0.0	10.3597975	1
CACCTTT	725	0.0	10.350732	14
>>END_MODULE