FastQCFastQC Report
Thu 26 May 2016
SRR1512856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922878
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT123700.643306543628873No Hit
TATCAACGCAGAGTACTTTTTTTTT91540.4760572433612533No Hit
GGTATCAACGCAGAGTACTTTTTTT70760.36799006489231245No Hit
GTCCTACAGTGGACATTTCTAAATT55250.2873297213863802No Hit
GTCCTAAAGTGTGTATTTCTCATTT54720.2845734362762484No Hit
CTGTAGGACGTGGAATATGGCAAGA49880.25940283262900715No Hit
CTTTAGGACGTGAAATATGGCGAGG49850.2592468164906978No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43200.22466323916545927No Hit
GTCCTACAGTGTGCATTTCTCATTT28830.1499315089152822No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28410.14774728297895134No Hit
CTGAAGGACCTGGAATATGGCGAGA25960.13500596501702136No Hit
CTGTAGGACCTGGAATATGGCGAGA22920.11919632966834089No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21560.11212359806498384No Hit
TTTCTAAATTTTCCACCTTTTTCAG20690.10759913005401278No Hit
ATTTAGAAATGTCCACTGTAGGACG20570.10697506550077539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19100.014.3425261
TAGGACC8250.013.716244
GGCGAGG13650.012.72467219
AGTCGTC2450.012.4097788
CTGGTCG701.091159E-412.2142869
GCGTGAG550.003083285612.08336211
CGAAATC1657.2759576E-1211.50646713
AAATGTC6800.011.4633417
GTATCAC1001.9013605E-611.4142591
AGGACCT16100.011.103865
GTTCGTC600.005914186611.07382218
AAGTCGT2750.011.0617437
CACCTTT7400.011.03221414
GCGCCAC1553.1650416E-1011.02393713
ACGAAAT1658.185452E-1110.93114412
TAGAAAT8100.010.9179214
GTATCAA45350.010.8647311
TGGCGAG30850.010.73794118
TGCGTTG2300.010.73103112
CCATCGC1158.9227615E-710.72935719
Produced by FastQC (version 0.11.5)