##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512856_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1922878 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.12449151740256 31.0 30.0 34.0 26.0 34.0 2 30.142377727552137 31.0 30.0 34.0 26.0 34.0 3 30.30537558805083 31.0 30.0 34.0 26.0 34.0 4 33.875675939919226 37.0 35.0 37.0 30.0 37.0 5 33.452199775544784 37.0 35.0 37.0 28.0 37.0 6 33.29533854981959 37.0 35.0 37.0 27.0 37.0 7 33.121702988957175 37.0 35.0 37.0 26.0 37.0 8 33.05789186833486 37.0 35.0 37.0 26.0 37.0 9 34.33155665622052 39.0 35.0 39.0 25.0 39.0 10 34.256596622354614 39.0 35.0 39.0 25.0 39.0 11 34.21838358959851 39.0 35.0 39.0 25.0 39.0 12 34.085715266387155 39.0 35.0 39.0 25.0 39.0 13 34.072218830315805 39.0 35.0 39.0 25.0 39.0 14 35.13125637715965 40.0 35.0 41.0 24.0 41.0 15 35.14128977501433 40.0 35.0 41.0 25.0 41.0 16 35.15058261626583 40.0 35.0 41.0 25.0 41.0 17 35.16275915580707 40.0 35.0 41.0 25.0 41.0 18 35.09682725581134 40.0 35.0 41.0 24.0 41.0 19 35.11812137847539 40.0 35.0 41.0 24.0 41.0 20 35.0933059715697 40.0 35.0 41.0 24.0 41.0 21 35.06213082681273 40.0 35.0 41.0 24.0 41.0 22 35.0247129563082 40.0 35.0 41.0 23.0 41.0 23 34.86759534406239 40.0 35.0 41.0 22.0 41.0 24 34.830619519283076 40.0 35.0 41.0 21.0 41.0 25 34.77599202861544 40.0 35.0 41.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7470756381655868 1101 2 0.616455069719553 1101 3 0.5956611853352349 1101 4 0.600639204902798 1101 5 0.7315191060474859 1101 6 0.7982242575098581 1101 7 0.8419728907528921 1101 8 0.650826214841679 1101 9 0.8842974861395305 1101 10 0.6282122110438664 1101 11 0.8477014928418498 1101 12 0.9945840594567485 1101 13 1.0655001503519514 1101 14 1.1253993314288024 1101 15 0.8525869551732725 1101 16 1.0714832831152208 1101 17 1.1082984628146946 1101 18 1.0674368319237146 1101 19 0.972253477389124 1101 20 1.0297918418390068 1101 21 1.0562678858388281 1101 22 0.8883098718824982 1101 23 1.0992194459808076 1101 24 1.0149993028664213 1101 25 1.047793332803515 1102 1 0.8057539977285586 1102 2 0.930475552627275 1102 3 0.551267464873554 1102 4 0.6945757447368166 1102 5 0.8654625121381301 1102 6 0.9244783821309923 1102 7 0.9150807170983342 1102 8 1.0363875360690002 1102 9 1.161447453066586 1102 10 1.0171774576236743 1102 11 1.0740556435319704 1102 12 1.0696102590216938 1102 13 1.0001915320602635 1102 14 1.0537979503900203 1102 15 1.186618112593365 1102 16 1.139845509966655 1102 17 1.1617064006062208 1102 18 0.6104216876967143 1102 19 1.0621319930810884 1102 20 1.188019288726423 1102 21 1.035056566107265 1102 22 0.9021514807328472 1102 23 1.1413089817772288 1102 24 1.1698568945197891 1102 25 1.1515064533841937 1103 1 0.7261963056102196 1103 2 0.8651244154182258 1103 3 0.667405513147262 1103 4 0.5591503552541397 1103 5 0.6368311358526455 1103 6 0.6196852441063072 1103 7 0.7340807535066034 1103 8 0.8498933576148673 1103 9 0.8190877296870482 1103 10 0.7548246907435754 1103 11 0.8212033757200174 1103 12 0.968260611117195 1103 13 0.8748301684764641 1103 14 0.9773641570072158 1103 15 1.0788627835323936 1103 16 0.9388427482043227 1103 17 0.9610361219638435 1103 18 1.0210938691867 1103 19 0.9026721041240364 1103 20 0.7634928424649203 1103 21 0.9914604039516774 1103 22 0.8138914093222525 1103 23 1.015571055871959 1103 24 0.9395988239614681 1103 25 0.9939876521736082 1104 1 0.9515430847609352 1104 2 1.0315917415752978 1104 3 0.9289935969849488 1104 4 0.728337779334737 1104 5 0.8705995515156104 1104 6 0.8047659070101716 1104 7 0.8510496310528666 1104 8 0.867093976837161 1104 9 0.8291607589827734 1104 10 0.9998168437944202 1104 11 1.1162959990544579 1104 12 1.0306928586781083 1104 13 0.9100427694634305 1104 14 1.1888844384040098 1104 15 1.249992277527511 1104 16 1.2798436909049258 1104 17 1.069639365013927 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-3.496918467407319 2216 22 -2.910484440794022 2216 23 -4.053174752310468 2216 24 -3.429610199650611 2216 25 -3.540436964059616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59548.0 3 1.0 4 997.0 5 8343.0 6 10758.0 7 8463.0 8 7129.0 9 5864.0 10 4840.0 11 4277.0 12 3768.0 13 3514.0 14 3317.0 15 3240.0 16 3304.0 17 3505.0 18 3889.0 19 4824.0 20 6500.0 21 8743.0 22 10846.0 23 12718.0 24 14746.0 25 16792.0 26 18572.0 27 19982.0 28 22411.0 29 26885.0 30 33453.0 31 42296.0 32 53333.0 33 68329.0 34 88777.0 35 116788.0 36 161292.0 37 251246.0 38 809588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.08755127110704 19.84389639592746 13.831695434008621 26.23685689895688 2 12.856679813971105 20.878616614301166 41.77252926146695 24.492174310260776 3 16.938934529834686 26.32668814571073 30.155100614098146 26.579276710356442 4 11.015328510050933 17.51110514745233 38.09784595765351 33.37572038484322 5 11.910800725312383 39.82440407764546 35.17655822005096 13.088236976991203 6 30.502067926224885 37.862875780806064 18.4446114234451 13.190444869523951 7 27.177093558370018 32.58918487352096 22.279745553377683 17.95397601473134 8 25.042590235622608 37.65606446449738 20.492901974292877 16.80844332558713 9 27.050660296827967 14.583594509551375 20.380328059871214 37.98541713374944 10 15.593022614479885 29.47411526089748 33.65395922504483 21.278902899577805 11 33.18860870885802 22.72906468504191 25.493880582421262 18.588446023678806 12 23.90422852518814 25.30572805531734 31.663311437232593 19.126731982261923 13 29.056190258746362 22.732816336422797 26.062073954724717 22.14891945010612 14 20.46108161238149 23.302719372604493 28.16004202052141 28.076156994492607 15 22.25019322530747 32.79114086671043 23.65062159775224 21.308044310229864 16 20.844441763049524 28.323869280610637 29.314760281560336 21.516928674779503 17 20.338767968021486 29.51808074824565 28.716485567074113 21.426665716658754 18 20.248776763499286 27.051781598195934 32.25734982067519 20.442091817629585 19 22.94429928938741 26.461894023864602 28.99298332473498 21.600823362013013 20 23.508988712623307 26.98433996097472 29.54901459079619 19.957656735605784 21 22.3584592646216 26.39184886789254 28.389015608927053 22.860676258558804 22 22.99056565014637 28.544927964336587 28.35323503913687 20.11127134638017 23 21.103782975310967 28.188527821317837 29.589552743335773 21.118136460035426 24 22.76738305810353 26.507453931034625 30.16124787948065 20.56391513138119 25 21.605698128377426 27.583875619709424 29.874037704959804 20.936388546953342 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 135.0 1 135.0 2 510.0 3 885.0 4 885.0 5 885.0 6 2304.5 7 3724.0 8 3724.0 9 3724.0 10 4479.5 11 5235.0 12 5235.0 13 5235.0 14 7190.0 15 9145.0 16 9145.0 17 9145.0 18 16913.5 19 24682.0 20 24682.0 21 24682.0 22 39830.0 23 54978.0 24 54978.0 25 54978.0 26 82105.0 27 109232.0 28 109232.0 29 109232.0 30 138878.5 31 168525.0 32 168525.0 33 168525.0 34 184662.0 35 200799.0 36 200799.0 37 200799.0 38 210057.5 39 219316.0 40 219316.0 41 219316.0 42 228519.0 43 237722.0 44 237722.0 45 237722.0 46 244127.5 47 250533.0 48 250533.0 49 250533.0 50 236581.5 51 222630.0 52 222630.0 53 222630.0 54 192529.0 55 162428.0 56 162428.0 57 162428.0 58 137850.5 59 113273.0 60 113273.0 61 113273.0 62 92592.0 63 71911.0 64 71911.0 65 71911.0 66 54830.0 67 37749.0 68 37749.0 69 37749.0 70 28081.0 71 18413.0 72 18413.0 73 18413.0 74 12973.5 75 7534.0 76 7534.0 77 7534.0 78 5163.0 79 2792.0 80 2792.0 81 2792.0 82 1861.0 83 930.0 84 930.0 85 930.0 86 588.5 87 247.0 88 247.0 89 247.0 90 149.5 91 52.0 92 52.0 93 52.0 94 30.0 95 8.0 96 8.0 97 8.0 98 4.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08934524187181922 2 0.04217636272296006 3 0.01997006570359638 4 0.006292650911810317 5 0.020594130256833768 6 0.05959816483417044 7 0.07967224129663972 8 0.055957788273619025 9 0.025222609026677723 10 0.05262944398968629 11 0.007592785397721541 12 4.680484149280401E-4 13 0.005564575599700033 14 4.160430354915913E-4 15 0.013105355617985124 16 0.008528882227577621 17 0.004524468010971055 18 0.00572059173800938 19 0.0015601613830934671 20 0.0011441183476018758 21 2.0802151774579564E-4 22 5.720591738009379E-4 23 0.0 24 0.0 25 5.200537943644891E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1922878.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.69886152035044 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3495668798024 42.52583952776848 2 13.705923685754861 15.268086907627024 3 4.564050820144513 7.626373038092132 4 1.9914679914902644 4.436899995209067 5 1.0077680829709152 2.806576744901302 6 0.5871073726692373 1.9620727348728373 7 0.3822997482421467 1.4905562515622914 8 0.2456735152832159 1.0946988085582032 9 0.17961965140214836 0.9004149080803693 >10 0.8814695357304562 8.65463609312732 >50 0.05940666955473373 2.300314430034141 >100 0.038242673185083474 4.353578028328664 >500 0.004217111641249742 1.629446355963439 >1k 0.002717694168805388 2.8836455756542967 >5k 3.7485436811108804E-4 1.4211787146047523 >10k+ 9.371359202777201E-5 0.6456818856157779 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12370 0.643306543628873 No Hit TATCAACGCAGAGTACTTTTTTTTT 9154 0.4760572433612533 No Hit GGTATCAACGCAGAGTACTTTTTTT 7076 0.36799006489231245 No Hit GTCCTACAGTGGACATTTCTAAATT 5525 0.2873297213863802 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5472 0.2845734362762484 No Hit CTGTAGGACGTGGAATATGGCAAGA 4988 0.25940283262900715 No Hit CTTTAGGACGTGAAATATGGCGAGG 4985 0.2592468164906978 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4320 0.22466323916545927 No Hit GTCCTACAGTGTGCATTTCTCATTT 2883 0.1499315089152822 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2841 0.14774728297895134 No Hit CTGAAGGACCTGGAATATGGCGAGA 2596 0.13500596501702136 No Hit CTGTAGGACCTGGAATATGGCGAGA 2292 0.11919632966834089 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2156 0.11212359806498384 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2069 0.10759913005401278 No Hit ATTTAGAAATGTCCACTGTAGGACG 2057 0.10697506550077539 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.200537943644891E-5 7 0.0 0.0 0.0 0.0 5.200537943644891E-5 8 0.0 0.0 0.0 0.0 5.200537943644891E-5 9 0.0 0.0 0.0 0.0 5.200537943644891E-5 10 0.0 0.0 0.0 0.0 5.200537943644891E-5 11 0.0 0.0 0.0 0.0 5.200537943644891E-5 12 0.0 0.0 0.0 0.0 5.200537943644891E-5 13 0.0 0.0 0.0 0.0 5.200537943644891E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1910 0.0 14.342526 1 TAGGACC 825 0.0 13.71624 4 GGCGAGG 1365 0.0 12.724672 19 AGTCGTC 245 0.0 12.409778 8 CTGGTCG 70 1.091159E-4 12.214286 9 GCGTGAG 55 0.0030832856 12.083362 11 CGAAATC 165 7.2759576E-12 11.506467 13 AAATGTC 680 0.0 11.463341 7 GTATCAC 100 1.9013605E-6 11.414259 1 AGGACCT 1610 0.0 11.10386 5 GTTCGTC 60 0.0059141866 11.073822 18 AAGTCGT 275 0.0 11.061743 7 CACCTTT 740 0.0 11.032214 14 GCGCCAC 155 3.1650416E-10 11.023937 13 ACGAAAT 165 8.185452E-11 10.931144 12 TAGAAAT 810 0.0 10.917921 4 GTATCAA 4535 0.0 10.864731 1 TGGCGAG 3085 0.0 10.737941 18 TGCGTTG 230 0.0 10.731031 12 CCATCGC 115 8.9227615E-7 10.729357 19 >>END_MODULE