FastQCFastQC Report
Thu 26 May 2016
SRR1512855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057951
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63660.30933681122631196No Hit
GTCCTACAGTGGACATTTCTAAATT43400.21088937491708987No Hit
TATCAACGCAGAGTACTTTTTTTTT38120.18523278736957294No Hit
GGTATCAACGCAGAGTACTTTTTTT36310.17643763141104915No Hit
CTGTAGGACGTGGAATATGGCAAGA35740.1736678861644422No Hit
GTCCTAAAGTGTGTATTTCTCATTT34750.16885727599928277No Hit
CTTTAGGACGTGAAATATGGCGAGG26660.12954633030621235No Hit
GTCCTACAGTGTGCATTTCTCATTT24500.11905045358222816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG350.002172209816.2838175
GGTATCA17200.013.7841731
GGACCGA500.001500601413.2990976
CGAGCAT604.0987306E-412.66457510
TAGGACC7350.012.1482444
CGGTTTC802.87155E-511.87332713
CCAACGA1001.9342242E-611.39811819
ACCGTGC1104.974918E-711.2265118
GGACCTG12350.011.153096
GGCGAGG8450.011.1283419
ATCCCGT1201.278786E-711.08150310
GTATCAA38050.011.0105191
AGGACCT12850.010.8664375
ATTAGAC1053.4722252E-610.8558783
TGTAGGA21700.010.8558772
GTAGGAC20600.010.7900053
TAATACG803.775852E-410.68625454
CGTGCGC803.7775503E-410.68573510
TGGCGAG19100.010.64273818
GTCCTAA12000.010.632451
Produced by FastQC (version 0.11.5)