##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512855_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2057951
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.69516572552019	31.0	30.0	34.0	18.0	34.0
2	28.777499561457002	31.0	30.0	34.0	16.0	34.0
3	28.896934377932226	31.0	30.0	34.0	20.0	34.0
4	32.31845801965159	35.0	33.0	37.0	22.0	37.0
5	32.50142301736047	35.0	33.0	37.0	25.0	37.0
6	32.46088803863649	36.0	33.0	37.0	23.0	37.0
7	32.35670722966679	36.0	33.0	37.0	22.0	37.0
8	32.278383693294934	36.0	33.0	37.0	22.0	37.0
9	33.57961001015087	38.0	34.0	39.0	19.0	39.0
10	33.43835640401545	38.0	33.0	39.0	18.0	39.0
11	33.52554458293711	38.0	34.0	39.0	18.0	39.0
12	33.42103966518153	38.0	34.0	39.0	17.0	39.0
13	33.3938602036686	38.0	34.0	39.0	17.0	39.0
14	34.216661621195065	39.0	33.0	41.0	17.0	41.0
15	34.397690712752635	39.0	34.0	41.0	17.0	41.0
16	34.34118207867923	39.0	34.0	41.0	17.0	41.0
17	34.09204689518847	39.0	33.0	41.0	16.0	41.0
18	34.09409650667096	39.0	33.0	41.0	16.0	41.0
19	34.230848547900315	39.0	34.0	41.0	17.0	41.0
20	34.20956038311894	39.0	34.0	41.0	16.0	41.0
21	34.10664345263808	39.0	34.0	41.0	10.0	41.0
22	34.10912844863653	39.0	34.0	41.0	10.0	41.0
23	33.77704279645142	39.0	34.0	41.0	2.0	41.0
24	33.85016358504163	39.0	34.0	41.0	2.0	41.0
25	33.56323887206255	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8600800399658048
1101	2	0.49083191257092906
1101	3	0.8756503184227036
1101	4	0.8826776870010065
1101	5	0.9092673107342222
1101	6	0.843725324963934
1101	7	0.5228297922570562
1101	8	0.8207366880187763
1101	9	0.8037982918398683
1101	10	0.8550086035701057
1101	11	0.9742630058510713
1101	12	0.8746547008939629
1101	13	1.1294719833663294
1101	14	1.1949573267757856
1101	15	0.9987776626856188
1101	16	0.8830102412303518
1101	17	0.43929233429647496
1101	18	0.8191867805356097
1101	19	0.4066296132208649
1101	20	-0.14270008888768615
1101	21	0.739269796089502
1101	22	0.7096789126884175
1101	23	0.28343334640006645
1101	24	0.8560164840662736
1101	25	0.5910303106614023
1102	1	1.0444870290270245
1102	2	1.0406781166487455
1102	3	0.9738560187463285
1102	4	1.07461696026256
1102	5	1.0062494493639065
1102	6	1.0877347577248244
1102	7	0.9141108765903283
1102	8	1.0404934620746786
1102	9	1.2065158472049404
1102	10	1.2830426754105702
1102	11	1.1051461121821546
1102	12	1.2326481640975544
1102	13	1.1423665616582
1102	14	1.2885895451321687
1102	15	1.3174850045150635
1102	16	1.2375509682360146
1102	17	1.4946577180848308
1102	18	1.5231352669390645
1102	19	1.2166180602599113
1102	20	1.477327952198607
1102	21	1.541479618235698
1102	22	1.3970920932515511
1102	23	1.2677255782759644
1102	24	0.685523986392937
1102	25	1.117812449875629
1103	1	0.511865598621057
1103	2	0.6266574636895186
1103	3	0.7308415695837063
1103	4	0.7929610838454053
1103	5	0.6503818489433684
1103	6	0.2532426049252905
1103	7	0.2257063411951563
1103	8	0.44494432146463225
1103	9	0.5796597168172752
1103	10	0.6442011263357301
1103	11	0.46489529713817035
1103	12	0.6483860636274841
1103	13	0.785752564751931
1103	14	0.725999171034097
1103	15	0.6684644594953184
1103	16	0.6545474219700225
1103	17	0.43413100568545104
1103	18	0.8146247329533622
1103	19	0.7633943920969557
1103	20	0.9191221404543413
1103	21	0.7650590085329654
1103	22	0.7589052425403295
1103	23	0.10451351310311452
1103	24	0.29638449407973866
1103	25	0.6816169918480455
1104	1	1.2343414213368433
1104	2	1.246302264748266
1104	3	1.2175826200978577
1104	4	1.3830142412648954
1104	5	1.1386029617245512
1104	6	1.0335104683906948
1104	7	1.2567896491573265
1104	8	1.0755020562952495
1104	9	1.3673610975019272
1104	10	1.2912139770128732
1104	11	1.0262049612106452
1104	12	1.3425046614430158
1104	13	1.2919318597229932
1104	14	1.6699136326767032
1104	15	1.3979221193926534
1104	16	1.4328101301219078
1104	17	1.7379188368891434
1104	18	1.47534361233879
1104	19	1.0060157846077047
1104	20	1.2151013879770076
1104	21	1.31091673452201
1104	22	1.028377793076288
1104	23	1.448205015237292
1104	24	1.381647802738037
1104	25	1.0471331613132762
1105	1	0.6858754271850955
1105	2	0.7519280804472928
1105	3	0.8530083255029659
1105	4	1.0538518063339808
1105	5	0.7497838697062278
1105	6	0.9315472052327181
1105	7	0.8994348071993841
1105	8	0.9333733601313838
1105	9	0.6700465025960582
1105	10	0.8155081392235957
1105	11	0.7851687018893543
1105	12	1.0027327306937508
1105	13	0.9701562456919106
1105	14	0.9694375279871608
1105	15	0.9867630027194707
1105	16	0.9362607484207857
1105	17	1.3384267238481087
1105	18	1.0473902410188245
1105	19	0.8346710012660239
1105	20	1.0631833201501806
1105	21	0.9204147162692351
1105	22	0.924460392362441
1105	23	1.1825363170752397
1105	24	1.3383737648400427
1105	25	1.5368515184846245
1106	1	0.7188496777937665
1106	2	0.7437795451689624
1106	3	0.6291733561439621
1106	4	0.7860566279881951
1106	5	0.6495502859703919
1106	6	0.8148903109198287
1106	7	0.7223749412440554
1106	8	0.7780148682470696
1106	9	0.9181951807876203
1106	10	0.7581517005260281
1106	11	0.651290167813876
1106	12	0.8062132434822971
1106	13	0.7866357092591585
1106	14	0.9867141496454224
1106	15	0.7352192420927821
1106	16	0.7852624995048458
1106	17	0.9747893677587527
1106	18	0.882462021173815
1106	19	0.8043998948675153
1106	20	0.9460397268907599
1106	21	0.9935855956341868
1106	22	0.8580384910686405
1106	23	1.0516097351746438
1106	24	0.7381355165751486
1106	25	1.0257870570749645
1107	1	1.030632353364215
1107	2	1.039024014726543
1107	3	1.0314789974706393
1107	4	1.0908513060085596
1107	5	1.123263181079956
1107	6	1.1877357541717686
1107	7	0.89820545507731
1107	8	1.0630995539256105
1107	9	1.03693450874065
1107	10	1.2541602254822735
1107	11	1.1699652759271189
1107	12	1.2581351485172831
1107	13	0.9715289261087676
1107	14	1.2951792519126641
1107	15	1.0544156596441
1107	16	1.159454197372277
1107	17	1.4696592828935024
1107	18	1.385868896259062
1107	19	1.1634402990594808
1107	20	1.2253205227676034
1107	21	0.9336002575831372
1107	22	1.0456977882650662
1107	23	1.4255044380639816
1107	24	1.160391127606978
1107	25	1.3645909514264574
1108	1	0.5167868273153999
1108	2	0.5343355260362657
1108	3	0.5944642070069293
1108	4	0.6267118592811229
1108	5	0.5716686890509024
1108	6	0.5553874401135062
1108	7	0.45204587661621787
1108	8	0.5987940095173769
1108	9	0.6569272395675156
1108	10	0.8346210683882376
1108	11	0.40083181950294033
1108	12	0.7041896018639449
1108	13	0.7160821196909026
1108	14	0.6838198525482042
1108	15	0.8331214433353722
1108	16	0.4945788931264872
1108	17	0.6474788710114723
1108	18	0.8300182997060332
1108	19	0.8732246865356643
1108	20	0.8417413764456967
1108	21	0.9009121060245349
1108	22	0.8740698865605268
1108	23	1.1352508890858246
1108	24	1.059883748098187
1108	25	1.1053364219509305
1109	1	0.9617183099526976
1109	2	0.8851088894167134
1109	3	1.0024961026079353
1109	4	1.075687735056924
1109	5	0.8460737764397734
1109	6	1.0586226600447546
1109	7	0.8916808189062451
1109	8	0.8815929571319288
1109	9	1.1218847924951802
1109	10	1.039674396492785
1109	11	0.9558706947982074
1109	12	0.9572890390328865
1109	13	1.1135479990937185
1109	14	1.185957763647778
1109	15	1.1618838225807693
1109	16	0.8569071394391798
1109	17	1.3032722381846327
1109	18	1.2808949500419473
1109	19	1.263379285566863
1109	20	1.0548971151315385
1109	21	1.1832846497373808
1109	22	1.1665814902331917
1109	23	1.4503184490649872
1109	24	1.1964489873428121
1109	25	0.9198857060305627
1110	1	0.5147911902864308
1110	2	0.5444758498503504
1110	3	0.5293938918979961
1110	4	0.5900107045583383
1110	5	0.3855852024092883
1110	6	0.49244708726401143
1110	7	0.4229039585905383
1110	8	0.5303520672894422
1110	9	0.632270580510486
1110	10	0.5799896748765931
1110	11	0.6269376590301619
1110	12	0.6382325396123605
1110	13	0.7340485980225964
1110	14	0.6101988656398518
1110	15	0.6955472384486328
1110	16	0.7207812133691434
1110	17	0.7024699606799985
1110	18	0.20812458205967488
1110	19	0.6653909084000915
1110	20	0.6717369755389555
1110	21	0.8756896942656383
1110	22	0.9253875049057925
1110	23	1.0363979153003129
1110	24	0.8698826702134426
1110	25	0.9940245778579282
1111	1	0.47061183233245885
1111	2	0.4836632402646437
1111	3	0.5970971590438552
1111	4	0.49331631442862545
1111	5	0.5200993533093836
1111	6	0.33212835923853135
1111	7	0.2985921338909563
1111	8	0.46938000189145157
1111	9	0.48202657731586385
1111	10	0.5075670848441831
1111	11	0.32152818715294984
1111	12	-0.02326975996627567
1111	13	0.4128752162190992
1111	14	0.4198443357912325
1111	15	0.45375760628941464
1111	16	0.5320141730783234
1111	17	0.05452884090912846
1111	18	0.3797273103421972
1111	19	0.3741438115607494
1111	20	0.5113941960322919
1111	21	0.4496177050612644
1111	22	0.19842469290208697
1111	23	0.512395179673824
1111	24	0.48524798650878154
1111	25	0.6175198455913034
1112	1	0.21364918257911114
1112	2	0.23967854279157308
1112	3	0.1928768831956731
1112	4	0.36346732620754096
1112	5	0.214347818022965
1112	6	0.05648152545216334
1112	7	-0.09075046149815336
1112	8	0.10301233854703895
1112	9	-0.12458471836519891
1112	10	0.09643348975710353
1112	11	-0.008063074298775064
1112	12	0.06546778156196353
1112	13	0.21992041907825666
1112	14	0.15344848358142826
1112	15	-0.21654643075881097
1112	16	0.049175151634649694
1112	17	0.4998293543491741
1112	18	0.21782254843743232
1112	19	0.34801071984626475
1112	20	0.025802733231522268
1112	21	0.07936702550825459
1112	22	-0.04478078379176509
1112	23	0.17082566391826504
1112	24	0.16071461290564315
1112	25	0.1153342017777561
1113	1	-0.10889200331717674
1113	2	0.11658271492511929
1113	3	0.1341429165264003
1113	4	0.1432022236593511
1113	5	0.03794905922737968
1113	6	-0.2628866944046351
1113	7	-0.09553685206208229
1113	8	-0.05635688093248348
1113	9	-0.07528418021328775
1113	10	0.02086098974990591
1113	11	-0.017037382415388436
1113	12	-0.33435418154285657
1113	13	-0.08127222559367198
1113	14	0.22647157403028473
1113	15	-0.1701215730773029
1113	16	-0.20772129293213482
1113	17	0.02874348815053196
1113	18	-0.1116385164896414
1113	19	-0.5956890603760883
1113	20	-0.29859788099761886
1113	21	-0.12642602973362926
1113	22	-0.4695260541873907
1113	23	-3.9754296387969497
1113	24	-1.6409517841911452
1113	25	-0.9199344575135697
1114	1	-0.6493680423153485
1114	2	-1.0136342124407776
1114	3	-0.7863908519963374
1114	4	-0.7014873196349711
1114	5	-0.7492531853600433
1114	6	-1.4348775633651165
1114	7	-1.2630017940027827
1114	8	-1.1524077043934113
1114	9	-1.3651754237191582
1114	10	-0.9567725160988303
1114	11	-1.21282348900138
1114	12	-2.253324960932293
1114	13	-1.3526782309662266
1114	14	-3.2163738230265473
1114	15	-1.774378211087388
1114	16	-1.854627193145589
1114	17	-1.8802598428928405
1114	18	-1.7367927287584877
1114	19	-1.7631490410641746
1114	20	-1.3574322084297918
1114	21	-1.2638490306066146
1114	22	-1.382595374949105
1114	23	-5.473107091615923
1114	24	-2.7584947539891758
1114	25	-2.7105044198333417
1115	1	-1.9787815549260976
1115	2	-1.8297886181310368
1115	3	-1.4947888434039456
1115	4	-1.4946330547567008
1115	5	-1.4916776024001557
1115	6	-1.814086292594098
1115	7	-2.2411811225115166
1115	8	-2.486854894905246
1115	9	-3.629023584183738
1115	10	-4.38446716595411
1115	11	-3.2706663669517155
1115	12	-3.4205772717681704
1115	13	-2.660417627186103
1115	14	-3.308525576511265
1115	15	-2.954087225263283
1115	16	-3.1876457115253842
1115	17	-2.978564221509778
1115	18	-5.825792203042486
1115	19	-3.6640726071051084
1115	20	-3.9836075063851446
1115	21	-3.615746842325759
1115	22	-2.9977276596547604
1115	23	-3.6170099864729153
1115	24	-3.2516951159399383
1115	25	-3.656969935302971
1116	1	-1.409868173247844
1116	2	-1.1267569763706327
1116	3	-1.6832324773397112
1116	4	-1.3928195260544705
1116	5	-1.4588273354791923
1116	6	-3.887187455933912
1116	7	-2.288883477201889
1116	8	-2.752789041385782
1116	9	-3.814409702615979
1116	10	-2.9660748590937125
1116	11	-2.53791826686437
1116	12	-2.194708281983555
1116	13	-3.668372180470584
1116	14	-6.655893409966243
1116	15	-4.504881696970077
1116	16	-3.4683409732551205
1116	17	-4.6094276925278415
1116	18	-3.2808129824243366
1116	19	-2.987686726639403
1116	20	-3.835038695859332
1116	21	-4.5533687527426885
1116	22	-4.04044370166509
1116	23	-3.170227263103172
1116	24	-4.079002110648254
1116	25	-3.917537501971797
1201	1	0.415916135872056
1201	2	0.5654539985259888
1201	3	0.928586454062561
1201	4	0.6795588953770846
1201	5	0.32631451070101036
1201	6	0.8590524155743537
1201	7	0.8684736249489191
1201	8	0.9766940603819805
1201	9	1.2716763393185673
1201	10	1.1983247585430377
1201	11	1.3091580083121812
1201	12	1.110688634580356
1201	13	1.2552066103151986
1201	14	1.36030250507293
1201	15	1.1953863696828861
1201	16	1.226351600356928
1201	17	1.478300951151823
1201	18	1.3431677914297353
1201	19	1.3460981317503666
1201	20	1.3151911833586922
1201	21	1.5787356459439081
1201	22	1.4805598126842625
1201	23	1.7838870211656683
1201	24	1.5770732200987183
1201	25	1.4838815231294546
1202	1	0.9354769567488859
1202	2	0.8137875140663553
1202	3	0.8378247360375148
1202	4	1.0087651548884509
1202	5	0.9040111731076195
1202	6	1.0745288308514276
1202	7	1.1310594490253578
1202	8	1.2113401429949846
1202	9	1.4767029614902043
1202	10	1.2544865778603196
1202	11	1.1091957951437479
1202	12	1.3363294641544385
1202	13	1.3809183079459046
1202	14	1.8720833560534729
1202	15	1.5561498056599632
1202	16	1.4069561247570164
1202	17	1.6430255747996512
1202	18	1.6539816199902972
1202	19	1.5407728252622448
1202	20	1.476897424108472
1202	21	1.5116224402068852
1202	22	1.5374333549857369
1202	23	1.5805368727260927
1202	24	1.6582202887594022
1202	25	1.9667009886820779
1203	1	1.2012772062726143
1203	2	1.1922104437930265
1203	3	1.2199673921148424
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2211	17	0.35495391071986404
2211	18	-0.09825386661508873
2211	19	0.02406210714457302
2211	20	0.060743663402234915
2211	21	0.1884635009036444
2211	22	0.24086134612372234
2211	23	0.3540199598992899
2211	24	-0.018099826088175064
2211	25	0.43668108253411475
2212	1	0.14103204430439575
2212	2	0.2122328028859286
2212	3	-0.2026561623473988
2212	4	0.1377789207877811
2212	5	-0.14288616691751344
2212	6	-0.20082432026587327
2212	7	0.059868816977981965
2212	8	0.10054472796996805
2212	9	0.15484565479010826
2212	10	-0.16284006547675745
2212	11	-0.18098002302767213
2212	12	0.15431868607044663
2212	13	0.15485386974948057
2212	14	0.1489897306262833
2212	15	-0.20131441938605832
2212	16	-0.10845380721724496
2212	17	0.0017612636587927
2212	18	0.2750325797853961
2212	19	0.024960724532832046
2212	20	0.17698570222724186
2212	21	0.30457180268777506
2212	22	0.2986030777356419
2212	23	0.22695257630018517
2212	24	0.23515170348146341
2212	25	0.3488036192888728
2213	1	-0.15730271688643782
2213	2	-0.05403674091359889
2213	3	-0.26467961490683223
2213	4	0.008827325157028554
2213	5	-0.0061438238033559855
2213	6	0.07872162104918345
2213	7	-0.10397515389372103
2213	8	0.047174091859453426
2213	9	-0.3665561443103371
2213	10	0.012114421565435407
2213	11	-0.07770576729839718
2213	12	-0.06629065211760121
2213	13	-0.1795057361618504
2213	14	-0.2366228202411449
2213	15	-0.15487781625905228
2213	16	-0.1333895536297547
2213	17	-0.1688534289948791
2213	18	-0.1842948993321869
2213	19	0.019009235991390483
2213	20	0.14794040344806092
2213	21	0.011080155654148882
2213	22	-0.2373949946567322
2213	23	-0.04898445518406902
2213	24	0.11775504466801578
2213	25	0.3484609334889157
2214	1	-3.153535174063684
2214	2	-1.8670235585190404
2214	3	-2.1449265381355502
2214	4	-3.8699331350838015
2214	5	-2.289659480705506
2214	6	-2.045558544399931
2214	7	-1.8998203647983622
2214	8	-1.6912124275937153
2214	9	-1.5382974239423959
2214	10	-1.660128207176367
2214	11	-1.5639567661866742
2214	12	-1.4494761141657122
2214	13	-1.8137348288684514
2214	14	-1.9018280978881208
2214	15	-2.1426112728130278
2214	16	-2.0400273494130303
2214	17	-4.0301801870870975
2214	18	-3.8031184482821097
2214	19	-2.6696456400487705
2214	20	-2.5088239019020726
2214	21	-2.4532133738943855
2214	22	-3.394596173198366
2214	23	-2.4516490690417037
2214	24	-3.466677147181059
2214	25	-3.4988364509380006
2215	1	-2.115672558537568
2215	2	-3.666484647452304
2215	3	-2.2907124285447367
2215	4	-3.8298585279174873
2215	5	-2.5479451804257565
2215	6	-2.3989671260006027
2215	7	-2.5049192227695727
2215	8	-1.814932180708027
2215	9	-4.212917895031925
2215	10	-2.4945239916667816
2215	11	-2.0896381725769473
2215	12	-2.0625582247243557
2215	13	-2.0911517942167492
2215	14	-1.7972007002040797
2215	15	-2.022669091628998
2215	16	-2.059465262158021
2215	17	-2.531368251562551
2215	18	-2.232198578379272
2215	19	-2.385549251668227
2215	20	-2.2210317205925207
2215	21	-2.5698644088959917
2215	22	-2.462280703604417
2215	23	-2.315347997958856
2215	24	-2.300056438961388
2215	25	-3.2662644057620085
2216	1	-3.028290886790309
2216	2	-2.9627878229345868
2216	3	-4.312231210223388
2216	4	-4.855787930959238
2216	5	-4.461296141985866
2216	6	-3.5199313577356115
2216	7	-5.1686735547806
2216	8	-3.101074173110405
2216	9	-3.6801839139044077
2216	10	-2.740866641169003
2216	11	-3.11043595302635
2216	12	-3.2807069682347816
2216	13	-2.895930814750745
2216	14	-3.899559235372731
2216	15	-3.3748280053279807
2216	16	-3.6976190787554195
2216	17	-3.314328682985071
2216	18	-3.0417589458628846
2216	19	-3.591133287053758
2216	20	-4.03541583582664
2216	21	-3.813518547439294
2216	22	-3.5215382618285958
2216	23	-3.6002346639951384
2216	24	-3.9731916667474962
2216	25	-4.017437181896284
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	112262.0
3	2.0
4	1020.0
5	5988.0
6	7065.0
7	6273.0
8	5599.0
9	5188.0
10	4699.0
11	4409.0
12	4145.0
13	4062.0
14	3935.0
15	4088.0
16	4188.0
17	4568.0
18	5054.0
19	6317.0
20	7803.0
21	10149.0
22	12795.0
23	15354.0
24	17676.0
25	20341.0
26	22351.0
27	24974.0
28	28098.0
29	32809.0
30	40073.0
31	49737.0
32	61822.0
33	78818.0
34	102262.0
35	134289.0
36	183797.0
37	286349.0
38	739592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.159857945653094	18.67266750721339	12.804247198746395	24.363227348387113
2	14.620129150645948	20.254870363386843	42.31651821988267	22.808482266084543
3	19.17736190147587	25.92986582562677	28.972382724988698	25.920389547908655
4	12.003180191064168	16.6544526076692	38.47205037764769	32.87031682361894
5	12.824329617544974	39.13999376004152	34.86474744205441	13.170929180359098
6	31.054869680496672	36.73232976081749	18.96716067178471	13.245639886901131
7	28.168151723856198	32.0042387594577	21.886170147578472	17.941439369107634
8	25.587155686612917	36.553864803152905	20.718100087656232	17.14087942257795
9	27.81093261912763	14.827176843238691	20.090634220692092	37.27125631694159
10	15.906090864432912	29.090820659231625	33.59129001035977	21.41179846597569
11	34.23542909613876	22.575885134047404	24.692755851974198	18.49592991783964
12	23.884038881344193	24.967070318732077	31.352218205590166	19.796672594333565
13	29.096655871520415	22.257195534807174	25.908205105509218	22.737943488163193
14	21.40576804666731	22.48894258314098	27.88837626441695	28.21691310577476
15	23.123765891427627	31.149581254194754	23.590173820848776	22.136479033528843
16	22.002957999999026	28.275941227137146	27.991035538312715	21.730065234551112
17	21.486625246674006	28.710640994897002	27.738975246746968	22.063758511682025
18	21.088519422906675	27.483437265908496	30.216458639375045	21.21158467180979
19	23.352292956825757	26.77630951755589	27.99292589112176	21.878471634496595
20	23.945265685069028	26.934465906084142	28.418049308160438	20.702219100686392
21	23.592242830755943	26.550787028976558	27.248136623911712	22.60883351635579
22	23.56880082848083	27.990559443450785	27.27376203357215	21.166877694496236
23	22.455972757999614	27.654219985670807	28.025825618706357	21.863981637623223
24	23.041047226148358	26.724179021430704	28.665257472925877	21.569516279495062
25	22.62357081575123	27.253378779301073	28.482910098304142	21.640140306643556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	308.0
1	308.0
2	681.5
3	1055.0
4	1055.0
5	1055.0
6	1839.0
7	2623.0
8	2623.0
9	2623.0
10	3235.5
11	3848.0
12	3848.0
13	3848.0
14	5727.5
15	7607.0
16	7607.0
17	7607.0
18	14684.5
19	21762.0
20	21762.0
21	21762.0
22	36176.5
23	50591.0
24	50591.0
25	50591.0
26	74906.5
27	99222.0
28	99222.0
29	99222.0
30	128690.5
31	158159.0
32	158159.0
33	158159.0
34	179499.5
35	200840.0
36	200840.0
37	200840.0
38	215355.0
39	229870.0
40	229870.0
41	229870.0
42	242231.5
43	254593.0
44	254593.0
45	254593.0
46	264544.5
47	274496.0
48	274496.0
49	274496.0
50	264288.0
51	254080.0
52	254080.0
53	254080.0
54	222973.5
55	191867.0
56	191867.0
57	191867.0
58	164624.0
59	137381.0
60	137381.0
61	137381.0
62	112558.0
63	87735.0
64	87735.0
65	87735.0
66	66964.5
67	46194.0
68	46194.0
69	46194.0
70	34037.0
71	21880.0
72	21880.0
73	21880.0
74	15415.5
75	8951.0
76	8951.0
77	8951.0
78	6169.0
79	3387.0
80	3387.0
81	3387.0
82	2222.0
83	1057.0
84	1057.0
85	1057.0
86	689.5
87	322.0
88	322.0
89	322.0
90	201.5
91	81.0
92	81.0
93	81.0
94	53.0
95	25.0
96	25.0
97	25.0
98	21.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3355279110144022
2	0.009669812352189144
3	0.008795155958523794
4	0.010350100658373305
5	0.011905045358222816
6	0.08246066111389436
7	0.08207192493893198
8	0.10656230396156177
9	0.08620224679790724
10	0.09334527401284093
11	0.08989524046004982
12	0.09985660494346074
13	0.08649379892912902
14	0.09062412078810428
15	0.08800015160710824
16	0.08902058406638448
17	0.10204324592762413
18	0.10354959860560335
19	0.09732981980620531
20	0.09635797936879936
21	0.08440434198870625
22	0.10398692680243601
23	0.09757277991555678
24	0.09373401018780331
25	0.09149877718176963
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2057951.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.205038576235125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34233065255829	43.09960931403059
2	13.850717146561777	15.846616173551753
3	4.7705029202115545	8.186904107362327
4	2.2165721771373588	5.071963876006082
5	1.1916691850947188	3.4084740851727027
6	0.6977996168186317	2.3950592399155144
7	0.45201354516123965	1.8100216601550656
8	0.2996612664656079	1.3713707446374857
9	0.20628283805392225	1.0620375937640896
>10	0.8892190778901589	8.494828444042762
>50	0.04702082040917273	1.8502364218538045
>100	0.02987833851064199	3.3185541075572154
>500	0.004535378401688592	1.7396132346237079
>1k	0.0017114635478070019	2.033081488600371
>5k	8.55731773903501E-5	0.31162950872652323
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6366	0.30933681122631196	No Hit
GTCCTACAGTGGACATTTCTAAATT	4340	0.21088937491708987	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3812	0.18523278736957294	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3631	0.17643763141104915	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3574	0.1736678861644422	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3475	0.16885727599928277	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2666	0.12954633030621235	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2450	0.11905045358222816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	4.8592021870297204E-5	0.0	0.0	0.0
5	0.0	4.8592021870297204E-5	0.0	0.0	0.0
6	0.0	4.8592021870297204E-5	0.0	0.0	4.8592021870297204E-5
7	0.0	4.8592021870297204E-5	0.0	0.0	4.8592021870297204E-5
8	0.0	4.8592021870297204E-5	0.0	0.0	4.8592021870297204E-5
9	0.0	4.8592021870297204E-5	0.0	0.0	4.8592021870297204E-5
10	0.0	4.8592021870297204E-5	0.0	0.0	4.8592021870297204E-5
11	0.0	4.8592021870297204E-5	0.0	0.0	4.8592021870297204E-5
12	0.0	4.8592021870297204E-5	0.0	0.0	4.8592021870297204E-5
13	0.0	4.8592021870297204E-5	0.0	4.8592021870297204E-5	4.8592021870297204E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACCG	35	0.0021722098	16.283817	5
GGTATCA	1720	0.0	13.784173	1
GGACCGA	50	0.0015006014	13.299097	6
CGAGCAT	60	4.0987306E-4	12.664575	10
TAGGACC	735	0.0	12.148244	4
CGGTTTC	80	2.87155E-5	11.873327	13
CCAACGA	100	1.9342242E-6	11.398118	19
ACCGTGC	110	4.974918E-7	11.226511	8
GGACCTG	1235	0.0	11.15309	6
GGCGAGG	845	0.0	11.12834	19
ATCCCGT	120	1.278786E-7	11.081503	10
GTATCAA	3805	0.0	11.010519	1
AGGACCT	1285	0.0	10.866437	5
ATTAGAC	105	3.4722252E-6	10.855878	3
TGTAGGA	2170	0.0	10.855877	2
GTAGGAC	2060	0.0	10.790005	3
TAATACG	80	3.775852E-4	10.6862545	4
CGTGCGC	80	3.7775503E-4	10.685735	10
TGGCGAG	1910	0.0	10.642738	18
GTCCTAA	1200	0.0	10.63245	1
>>END_MODULE