FastQCFastQC Report
Thu 26 May 2016
SRR1512855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057951
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79700.3872784143062687No Hit
TATCAACGCAGAGTACTTTTTTTTT57500.2794041257542089No Hit
GGTATCAACGCAGAGTACTTTTTTT46410.22551557350004933No Hit
GTCCTACAGTGGACATTTCTAAATT40210.19538851994046505No Hit
CTGTAGGACGTGGAATATGGCAAGA34880.16948897228359663No Hit
GTCCTAAAGTGTGTATTTCTCATTT32870.15972197588766693No Hit
CTTTAGGACGTGAAATATGGCGAGG29670.1441725288891718No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27070.1315386032028945No Hit
GTCCTACAGTGTGCATTTCTCATTT22670.11015811357996375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17250.014.55507851
TGGACCG551.9440927E-413.8319925
TAAACCG500.001488586913.3132925
TAAGACG500.001488586913.3132924
TGTTCGT500.001502571313.29678310
TAGGACC7900.012.8799474
TTGGACC1156.980008E-811.57677654
GGCGAGG8650.011.52087919
AGGACCT13850.010.9170365
AAATGTC4950.010.7548137
GTATCAA38500.010.7208381
GGACCTG13550.010.6674776
TGGCGAG20950.010.64727518
GTCCTAC18900.010.6174541
AGACCCG1002.3731007E-510.46044355
GTCCTAA10600.010.407631
GCGTGCG1659.313226E-1010.362399
GGCGTGC1951.4551915E-1110.2320268
GACGTGA10450.010.1887697
AGGACGA1502.4077963E-810.1434615
Produced by FastQC (version 0.11.5)