##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512855_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2057951 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.00853178719999 31.0 30.0 34.0 26.0 34.0 2 30.03865884075957 31.0 30.0 34.0 26.0 34.0 3 30.210197424525656 31.0 30.0 34.0 26.0 34.0 4 33.78781273217876 37.0 35.0 37.0 30.0 37.0 5 33.33620625563971 37.0 35.0 37.0 27.0 37.0 6 33.19228640526426 37.0 35.0 37.0 26.0 37.0 7 33.0170460812721 37.0 35.0 37.0 26.0 37.0 8 32.96075028025449 37.0 35.0 37.0 26.0 37.0 9 34.261393978768204 39.0 35.0 39.0 25.0 39.0 10 34.181709379863754 39.0 35.0 39.0 25.0 39.0 11 34.15489678811595 39.0 35.0 39.0 25.0 39.0 12 34.01790032901658 39.0 35.0 39.0 24.0 39.0 13 33.995670936771575 39.0 35.0 39.0 24.0 39.0 14 35.04782378200453 39.0 35.0 41.0 24.0 41.0 15 35.05026844662482 39.0 35.0 41.0 24.0 41.0 16 35.050357855945066 39.0 35.0 41.0 24.0 41.0 17 35.03984302833255 39.0 35.0 41.0 24.0 41.0 18 34.967051693650625 39.0 35.0 41.0 23.0 41.0 19 34.985214419585304 40.0 35.0 41.0 23.0 41.0 20 34.95232053630043 40.0 35.0 41.0 22.0 41.0 21 34.92236501257805 40.0 35.0 41.0 22.0 41.0 22 34.88439229116728 40.0 35.0 41.0 22.0 41.0 23 34.72463727270475 39.0 35.0 41.0 19.0 41.0 24 34.67054754948004 39.0 35.0 41.0 18.0 41.0 25 34.61221574274606 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8151821451491941 1101 2 0.7305151989262022 1101 3 0.6850512756357254 1101 4 0.6682634775525642 1101 5 0.8133887024865416 1101 6 0.7963524514895042 1101 7 0.9889641075009834 1101 8 0.7800175058482779 1101 9 1.1025802434418424 1101 10 0.7608944034091607 1101 11 1.1032005464682655 1101 12 1.1659546008591732 1101 13 1.2268114942689081 1101 14 1.3882891103111845 1101 15 1.0944186462535654 1101 16 1.3007868902879665 1101 17 1.416012796336794 1101 18 1.3236569231581896 1101 19 1.1902908901695994 1101 20 1.3907317026558914 1101 21 1.3468656355978297 1101 22 1.285918951740932 1101 23 1.3931490655841543 1101 24 1.2883245097641662 1101 25 1.361946930901837 1102 1 0.7115078902149712 1102 2 0.7889635991104171 1102 3 0.48149484670731013 1102 4 0.57212912667908 1102 5 0.7604061429900497 1102 6 0.6775247993709925 1102 7 0.6473730722980591 1102 8 0.6996567879196434 1102 9 0.8778826683945695 1102 10 0.7934941494694812 1102 11 0.8996576871542672 1102 12 0.8835291721533025 1102 13 1.0296023046755067 1102 14 0.9766398259726472 1102 15 1.0728113316886123 1102 16 1.0389601362691678 1102 17 1.1073540671290942 1102 18 0.48643791333921627 1102 19 0.8332362355204666 1102 20 1.0382464590896632 1102 21 0.8781151006418781 1102 22 0.7628773289319568 1102 23 1.0036410079471096 1102 24 0.8787277861788851 1102 25 0.8722249743889066 1103 1 0.7036393036108848 1103 2 0.8422066156780161 1103 3 0.6309061931847815 1103 4 0.5031430859780244 1103 5 0.6093926517651269 1103 6 0.5468274356566951 1103 7 0.6984232024206207 1103 8 0.7574448637742606 1103 9 0.6942885014846141 1103 10 0.8031663342312072 1103 11 0.8683340525029948 1103 12 0.9968145857360469 1103 13 1.0835511647639464 1103 14 1.0539886547081991 1103 15 1.0393696620847166 1103 16 1.0359362166778325 1103 17 0.9253259306346777 1103 18 0.9568809554894813 1103 19 0.9819230760430031 1103 20 0.9276391552384027 1103 21 1.1197968502541542 1103 22 0.9744467871826785 1103 23 1.0706163479110913 1103 24 0.9581185878383707 1103 25 0.9849945986444979 1104 1 0.9200738558135342 1104 2 1.0108410977082336 1104 3 0.8681974411939279 1104 4 0.8261577241719138 1104 5 0.9348531233606892 1104 6 0.9021642013315727 1104 7 0.747446889417958 1104 8 0.9599945555796552 1104 9 1.0358216211534241 1104 10 0.9841998133637091 1104 11 1.0906534474997827 1104 12 1.1730094074584514 1104 13 1.0789091421975456 1104 14 1.3215416651016412 1104 15 1.3543314733176643 1104 16 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2215 8 -1.73414382462407 2215 9 -1.8094950433610038 2215 10 -1.8239362020889942 2215 11 -1.6435474524085762 2215 12 -1.9347661172521882 2215 13 -1.8997846372818614 2215 14 -2.0913734121056606 2215 15 -1.9365009089576404 2215 16 -1.893116291475721 2215 17 -2.157169498359515 2215 18 -1.730640111180918 2215 19 -2.0233465559555555 2215 20 -2.088396395776279 2215 21 -1.844260834420119 2215 22 -2.069628477834577 2215 23 -2.48423552739677 2215 24 -2.533000073256396 2215 25 -2.7983018206338492 2216 1 -1.7038169800386669 2216 2 -1.0802325731868372 2216 3 -1.0414613803003405 2216 4 -1.3001219172504719 2216 5 -2.2149313377612216 2216 6 -2.3491243211266344 2216 7 -2.1990468004130257 2216 8 -2.416032941651782 2216 9 -4.376253228216488 2216 10 -2.4137756193744124 2216 11 -2.5090954508656402 2216 12 -2.8981678472048635 2216 13 -2.862636845350469 2216 14 -2.8354404894222043 2216 15 -2.7827087013993363 2216 16 -2.1856262838060587 2216 17 -2.50753407020148 2216 18 -3.0035945164365145 2216 19 -3.1017052997511527 2216 20 -2.44772085721106 2216 21 -3.130865108120666 2216 22 -2.629557131920592 2216 23 -3.7984504146556652 2216 24 -2.966317121680362 2216 25 -3.0853284138158017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68230.0 3 0.0 4 1152.0 5 9683.0 6 11394.0 7 8814.0 8 6978.0 9 6030.0 10 5108.0 11 4527.0 12 3917.0 13 3780.0 14 3628.0 15 3663.0 16 3661.0 17 3999.0 18 4176.0 19 5124.0 20 6818.0 21 8919.0 22 11114.0 23 13746.0 24 15646.0 25 17839.0 26 20042.0 27 21430.0 28 24446.0 29 29190.0 30 36380.0 31 46161.0 32 58672.0 33 75199.0 34 97609.0 35 128171.0 36 175615.0 37 272554.0 38 844536.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.8570167567607 19.604899364599977 13.873800266026645 26.664283612612678 2 13.055420248258912 20.757289822395805 41.068454473064776 25.118835456280507 3 17.01645681525802 25.839498786673776 29.861482031933832 27.28256236613437 4 11.219456725521889 17.42160871369577 37.63603000848472 33.72290455229763 5 12.418276511653676 39.21172191365645 35.08379877407477 13.286202800615103 6 31.131543850624364 37.39564092553147 18.37599752226967 13.096817701574496 7 27.772771504671724 32.180766606575176 21.946750220905514 18.099711667847586 8 25.359140600500297 37.025114438310275 20.398485986624756 17.217258974564672 9 27.104305016386892 14.68010928159647 20.026372617970807 38.18921308404583 10 15.28614670165814 29.590029957965502 33.55593563461589 21.567887705760466 11 33.922065729068414 22.653246580954228 24.676513051976105 18.748174638001256 12 23.382477780040013 25.302886809038327 31.38521060066173 19.92942481025993 13 28.78620593134844 22.194398710863773 26.047228804186197 22.972166553601593 14 20.98713420284489 22.48567375969235 27.988757673339855 28.538434364122907 15 22.97590668853654 31.18629487880445 23.52872850981107 22.309069922847943 16 21.88604369073584 28.304602728435523 27.826018497999744 21.983335082828894 17 21.09085784540025 28.774971693482943 27.854147329954372 22.280023131162437 18 20.90251863745062 27.43900216685724 30.37147581978784 21.287003375904295 19 23.15467941229637 26.72087381943216 28.224002239167156 21.900444529104313 20 23.731925960336728 26.98471858047236 28.472771589232643 20.81058386995827 21 23.220322428755317 26.346599492794503 27.621464734446 22.811613344004183 22 23.22589579871133 27.93288434065133 27.558286441781586 21.282933418855748 23 22.05572435884042 27.607897369762448 28.27224749277315 22.06413077862398 24 22.76541083825611 26.73537902505939 28.8689575213404 21.630252615344098 25 22.272007448185114 27.199238465833247 28.71506658807717 21.813687497904468 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 115.0 1 115.0 2 358.0 3 601.0 4 601.0 5 601.0 6 1466.5 7 2332.0 8 2332.0 9 2332.0 10 2969.5 11 3607.0 12 3607.0 13 3607.0 14 5615.0 15 7623.0 16 7623.0 17 7623.0 18 14822.0 19 22021.0 20 22021.0 21 22021.0 22 37392.5 23 52764.0 24 52764.0 25 52764.0 26 77787.0 27 102810.0 28 102810.0 29 102810.0 30 132319.0 31 161828.0 32 161828.0 33 161828.0 34 181998.5 35 202169.0 36 202169.0 37 202169.0 38 216203.0 39 230237.0 40 230237.0 41 230237.0 42 243109.0 43 255981.0 44 255981.0 45 255981.0 46 265016.5 47 274052.0 48 274052.0 49 274052.0 50 263231.5 51 252411.0 52 252411.0 53 252411.0 54 221410.5 55 190410.0 56 190410.0 57 190410.0 58 162862.5 59 135315.0 60 135315.0 61 135315.0 62 110554.0 63 85793.0 64 85793.0 65 85793.0 66 65067.5 67 44342.0 68 44342.0 69 44342.0 70 32724.0 71 21106.0 72 21106.0 73 21106.0 74 14686.5 75 8267.0 76 8267.0 77 8267.0 78 5627.0 79 2987.0 80 2987.0 81 2987.0 82 1956.0 83 925.0 84 925.0 85 925.0 86 568.5 87 212.0 88 212.0 89 212.0 90 124.5 91 37.0 92 37.0 93 37.0 94 21.5 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08581351062294486 2 0.04397577979261897 3 0.01865933639819413 4 0.006462738908749529 5 0.01958258481372977 6 0.059768186900465564 7 0.07978809991102802 8 0.056658297500766536 9 0.024976299241332763 10 0.05194487137934771 11 0.006802883061841608 12 1.9436808748118882E-4 13 0.006754291039971311 14 6.802883061841608E-4 15 0.015840999129716888 16 0.0104472847021139 17 0.005879634646305962 18 0.006414146886879231 19 0.00208945694042278 20 0.0013119845904980246 21 3.8873617496237763E-4 22 5.345122405732692E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2057951.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.01832321585016 #Duplication Level Percentage of deduplicated Percentage of total 1 74.31214438927523 41.62841723261346 2 14.255384579020443 15.971254818276265 3 5.1250244421247615 8.612858270642313 4 2.341032916906515 5.245629543928546 5 1.2530447310116366 3.509673237286394 6 0.7219585827627119 2.426574554259522 7 0.46213877809292175 1.812176760925114 8 0.3127203567902828 1.4014456018283237 9 0.20453736592379912 1.0312056246634196 >10 0.9254401262851024 8.71951740375383 >50 0.0488244601300434 1.8906528589256213 >100 0.031141200098989068 3.34144294138371 >500 0.004782157062815316 1.8216412560934887 >1k 0.0016520178944270936 1.919250054949394 >5k 1.7389662046600986E-4 0.668259840470655 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7970 0.3872784143062687 No Hit TATCAACGCAGAGTACTTTTTTTTT 5750 0.2794041257542089 No Hit GGTATCAACGCAGAGTACTTTTTTT 4641 0.22551557350004933 No Hit GTCCTACAGTGGACATTTCTAAATT 4021 0.19538851994046505 No Hit CTGTAGGACGTGGAATATGGCAAGA 3488 0.16948897228359663 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3287 0.15972197588766693 No Hit CTTTAGGACGTGAAATATGGCGAGG 2967 0.1441725288891718 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2707 0.1315386032028945 No Hit GTCCTACAGTGTGCATTTCTCATTT 2267 0.11015811357996375 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.8592021870297204E-5 2 0.0 0.0 0.0 0.0 4.8592021870297204E-5 3 0.0 0.0 0.0 0.0 4.8592021870297204E-5 4 0.0 0.0 0.0 0.0 4.8592021870297204E-5 5 0.0 0.0 0.0 0.0 4.8592021870297204E-5 6 0.0 0.0 0.0 0.0 4.8592021870297204E-5 7 0.0 0.0 0.0 0.0 4.8592021870297204E-5 8 0.0 0.0 0.0 0.0 4.8592021870297204E-5 9 0.0 0.0 0.0 0.0 4.8592021870297204E-5 10 0.0 0.0 0.0 0.0 4.8592021870297204E-5 11 0.0 0.0 0.0 0.0 4.8592021870297204E-5 12 0.0 0.0 0.0 0.0 4.8592021870297204E-5 13 0.0 0.0 0.0 0.0 4.8592021870297204E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1725 0.0 14.5550785 1 TGGACCG 55 1.9440927E-4 13.831992 5 TAAACCG 50 0.0014885869 13.313292 5 TAAGACG 50 0.0014885869 13.313292 4 TGTTCGT 50 0.0015025713 13.296783 10 TAGGACC 790 0.0 12.879947 4 TTGGACC 115 6.980008E-8 11.5767765 4 GGCGAGG 865 0.0 11.520879 19 AGGACCT 1385 0.0 10.917036 5 AAATGTC 495 0.0 10.754813 7 GTATCAA 3850 0.0 10.720838 1 GGACCTG 1355 0.0 10.667477 6 TGGCGAG 2095 0.0 10.647275 18 GTCCTAC 1890 0.0 10.617454 1 AGACCCG 100 2.3731007E-5 10.4604435 5 GTCCTAA 1060 0.0 10.40763 1 GCGTGCG 165 9.313226E-10 10.36239 9 GGCGTGC 195 1.4551915E-11 10.232026 8 GACGTGA 1045 0.0 10.188769 7 AGGACGA 150 2.4077963E-8 10.143461 5 >>END_MODULE