FastQCFastQC Report
Thu 26 May 2016
SRR1512854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654082
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT54380.3287624192754652No Hit
GTCCTAAAGTGTGTATTTCTCATTT50050.30258475698302745No Hit
GTATCAACGCAGAGTACTTTTTTTT48770.29484632563560936No Hit
CTGTAGGACGTGGAATATGGCAAGA43610.2636507742663302No Hit
CTTTAGGACGTGAAATATGGCGAGG36620.22139168433003925No Hit
TATCAACGCAGAGTACTTTTTTTTT28460.17205918449024898No Hit
GTCCTACAGTGTGCATTTCTCATTT27690.16740403438281778No Hit
GGTATCAACGCAGAGTACTTTTTTT27060.16359527520401043No Hit
CTGAAGGACCTGGAATATGGCGAGA19640.11873655598694623No Hit
GTCCTTCAGTGTGCATTTCTCATTT19270.1164996656755832No Hit
CTGTAGGACCTGGAATATGGCGAGA17740.1072498219556225No Hit
TTTCTAAATTTTCCACCTTTTTCAG17080.10325969329211006No Hit
ATTTAGAAATGTCCACTGTAGGACG16710.10102280298074702No Hit
GTACTGGTTCACTATCGGTCAGTCA16570.10017641205212317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7650.012.9129964
GGTATCA12550.012.6701081
TAGAAAT7350.012.1477444
AAATGTC7150.012.0893697
GCACCGC550.003071517212.0893686
ATTTAGA7350.011.5294931
AATGTCC7750.011.5211138
TCCAACG1001.9311847E-611.39923818
CTACACT1751.8189894E-1211.3982034
GGCGAGG10750.011.31087119
GAAATGT7250.011.267536
GTATCAA29350.011.192281
TGGACCG600.005882823411.0815865
ATAGGAC2250.010.9760473
TTTAGAA8700.010.9178182
TCGAACT2100.010.85641619
GGACCGT1053.4713357E-610.855766
CACCTTT7050.010.77844214
GTCCTAC25700.010.744161
TAAGCTG1158.833831E-710.7374375
Produced by FastQC (version 0.11.5)