FastQCFastQC Report
Thu 26 May 2016
SRR1512854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654082
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60830.36775685848706413No Hit
GTCCTACAGTGGACATTTCTAAATT49430.2988364542991218No Hit
GTCCTAAAGTGTGTATTTCTCATTT46450.2808204188184141No Hit
TATCAACGCAGAGTACTTTTTTTTT44250.2675199899400392No Hit
CTGTAGGACGTGGAATATGGCAAGA43960.26576675158788987No Hit
CTTTAGGACGTGAAATATGGCGAGG41670.2519222142553997No Hit
GGTATCAACGCAGAGTACTTTTTTT36160.21861068556456087No Hit
GTCCTACAGTGTGCATTTCTCATTT25570.15458725746365656No Hit
CTGAAGGACCTGGAATATGGCGAGA22420.13554346156961988No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20250.12242440217595017No Hit
CTGTAGGACCTGGAATATGGCGAGA19130.11565327474695934No Hit
ATTTAGAAATGTCCACTGTAGGACG17740.1072498219556225No Hit
TTTCTAAATTTTCCACCTTTTTCAG17310.10465019267484925No Hit
GTCCTTCAGTGTGCATTTCTCATTT16560.10011595555722147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGA350.002180038316.27477813
GAACCGC400.005245696314.2637196
ACCGTGC400.00527739514.25077158
CCCCCGC400.00530712514.23870919
TAGGACC8000.014.1457114
GGTATCA15950.012.9383821
GCACCGT853.9015413E-612.3059536
TCCAACG1052.7471106E-711.7529418
AGGACCT16100.011.6951855
ACCTTTT6450.011.62784615
GGACCTG16500.011.41097456
TAGAAAT7050.011.3307314
AATGTCC6850.011.2341858
CACCTTT6450.011.18628214
GCGCCCC600.005884147211.0812519
CCAACGT600.00591069411.07455319
TGTACTG2700.010.9185695
ATTTAGA8050.010.8685821
ACATCGT700.001479564510.8675956
AAATGTC6750.010.8432017
Produced by FastQC (version 0.11.5)