##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512854_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1654082 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.325956633347076 31.0 30.0 34.0 26.0 34.0 2 30.32219744849409 31.0 30.0 34.0 26.0 34.0 3 30.513351816899043 31.0 31.0 34.0 27.0 34.0 4 34.123424352601624 37.0 35.0 37.0 30.0 37.0 5 33.74175343181293 37.0 35.0 37.0 28.0 37.0 6 33.62928923717204 37.0 35.0 37.0 28.0 37.0 7 33.46374424000745 37.0 35.0 37.0 28.0 37.0 8 33.40689457959158 37.0 35.0 37.0 27.0 37.0 9 34.74371222224775 39.0 35.0 39.0 27.0 39.0 10 34.64162054843714 39.0 35.0 39.0 27.0 39.0 11 34.64833121937123 39.0 35.0 39.0 27.0 39.0 12 34.53059400924501 39.0 35.0 39.0 27.0 39.0 13 34.524535059325956 39.0 35.0 39.0 27.0 39.0 14 35.595892464823386 40.0 36.0 41.0 27.0 41.0 15 35.61120488585209 40.0 36.0 41.0 27.0 41.0 16 35.61614115866082 40.0 36.0 41.0 27.0 41.0 17 35.60857321462902 40.0 36.0 41.0 27.0 41.0 18 35.533872564963524 40.0 36.0 41.0 27.0 41.0 19 35.5426254562954 40.0 36.0 41.0 26.0 41.0 20 35.52657123407425 40.0 36.0 41.0 26.0 41.0 21 35.48938383949526 40.0 36.0 41.0 26.0 41.0 22 35.45266498275176 40.0 36.0 41.0 26.0 41.0 23 35.301098131773394 40.0 35.0 41.0 25.0 41.0 24 35.26817957029942 40.0 36.0 41.0 25.0 41.0 25 35.20202142336353 40.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.714799924522719 1101 2 0.4564102345696597 1101 3 0.5166829023481405 1101 4 0.530800595995295 1101 5 0.5706892090268525 1101 6 0.7349732683661188 1101 7 0.7125048017752746 1101 8 0.5002517284366235 1101 9 0.8836798765579204 1101 10 0.6059346303255211 1101 11 0.802966421766449 1101 12 0.9854189330630021 1101 13 1.0366748915165758 1101 14 1.2029763895240961 1101 15 0.9329888156395398 1101 16 1.094110701452685 1101 17 1.2311247657058004 1101 18 1.1389014542323608 1101 19 0.9280959090922636 1101 20 1.0015596092580878 1101 21 1.1304884362890633 1101 22 1.0409643645904794 1101 23 1.2638845855356706 1101 24 1.248292911242686 1101 25 1.3767527898664653 1102 1 0.8409653358091838 1102 2 0.8981103780416824 1102 3 0.6479735527546424 1102 4 0.6489193190942899 1102 5 0.8813019541248934 1102 6 0.773287472096392 1102 7 0.8195938142095187 1102 8 0.7817375605743564 1102 9 0.756291067375713 1102 10 0.7801443385990794 1102 11 0.8576711085191988 1102 12 0.8784539641486049 1102 13 0.9718661875471852 1102 14 0.9498812795288742 1102 15 1.0938400829757455 1102 16 1.086345577110265 1102 17 1.1519189431328698 1102 18 0.48428285069338983 1102 19 0.885625799097042 1102 20 1.0406868211184417 1102 21 0.8573392253851964 1102 22 0.6469886950543682 1102 23 0.8645828160473812 1102 24 0.8996729327634938 1102 25 0.9369712858014196 1103 1 0.7100080300697691 1103 2 0.9047723600890123 1103 3 0.6453322208115857 1103 4 0.46131448184703316 1103 5 0.6897824373962678 1103 6 0.5296638434809253 1103 7 0.8322788377837327 1103 8 0.9732278928800966 1103 9 0.915330871528397 1103 10 0.8315735252887109 1103 11 1.0577153101693924 1103 12 1.1650398966900823 1103 13 1.0994593510252884 1103 14 1.0842432457089473 1103 15 1.2638554968845312 1103 16 1.2013517540075327 1103 17 1.0993702646488757 1103 18 1.1011304796715322 1103 19 1.1070579325633574 1103 20 0.9733257729726503 1103 21 1.0126139556417897 1103 22 0.8684590799487424 1103 23 1.0540106880212718 1103 24 0.9717479735650159 1103 25 0.9191815383175168 1104 1 0.6164351446485057 1104 2 0.7487742745252639 1104 3 0.6620695098213183 1104 4 0.4600051834906722 1104 5 0.5868490314463912 1104 6 0.6119322028833238 1104 7 0.6234407988156008 1104 8 0.6300408516330691 1104 9 0.6186188332726772 1104 10 0.7373367760894922 1104 11 0.9532068953143593 1104 12 0.9415565579242653 1104 13 0.7608258349128008 1104 14 0.8423030635899451 1104 15 1.0201956228907463 1104 16 1.017151766935477 1104 17 0.942711891122002 1104 18 0.509060536733287 1104 19 0.9947107814562983 1104 20 0.9819591653069253 1104 21 0.9250722320715283 1104 22 0.836080901771389 1104 23 0.8217758175001535 1104 24 1.0533835512722831 1104 25 0.8138449097332767 1105 1 0.6049664062097335 1105 2 0.645185669271843 1105 3 0.66724894008399 1105 4 0.521418428033769 1105 5 0.4507040073990325 1105 6 0.5453395280775553 1105 7 0.5057937399920718 1105 8 0.5368111069049917 1105 9 0.7717819853259513 1105 10 0.43101047198294395 1105 11 0.7567585046373324 1105 12 0.707372318190636 1105 13 0.800411480491789 1105 14 0.7868091679184701 1105 15 0.8914497849329166 1105 16 0.8392013414822799 1105 17 0.6915095233824573 1105 18 0.6000705256345071 1105 19 0.7793944662509702 1105 20 0.7300168789584234 1105 21 0.7208546960005009 1105 22 0.6344530808316904 1105 23 0.7929189917509873 1105 24 0.8021811835327384 1105 25 0.7650102815423807 1106 1 0.029782477819583875 1106 2 0.32374370297424093 1106 3 -1.6710017513066617 1106 4 -0.3987489413856693 1106 5 -0.06721268422183613 1106 6 0.08980409162538905 1106 7 0.23207482496250265 1106 8 0.27650034958428193 1106 9 0.6483808062983556 1106 10 0.4036712864116083 1106 11 0.5714025218092189 1106 12 0.643735495049448 1106 13 0.6105174434627259 1106 14 0.5948338443315038 1106 15 0.5925426302767605 1106 16 0.6868452189106407 1106 17 0.7492117065671025 1106 18 0.7244078588608076 1106 19 0.7092224033179662 1106 20 0.7599878301446097 1106 21 0.7073399254495953 1106 22 0.6387762103391097 1106 23 0.577967114069466 1106 24 0.6930852391956819 1106 25 0.6735993264144895 1107 1 0.5221776448416158 1107 2 0.6541521005468169 1107 3 0.5078184334836706 1107 4 0.3714987213993055 1107 5 0.3824237489966862 1107 6 0.31647294515991575 1107 7 0.47230225921978786 1107 8 0.33283953278913714 1107 9 0.5715014667324496 1107 10 0.43391566889479805 1107 11 0.42031182146479296 1107 12 0.5797477415094576 1107 13 0.5146451565456616 1107 14 0.6551203240209702 1107 15 0.5932861653486583 1107 16 0.7176745885459823 1107 17 0.7184206071192918 1107 18 0.833488827643265 1107 19 0.6255145688638635 1107 20 0.7061362091288075 1107 21 0.6407060618008131 1107 22 0.5410505529119618 1107 23 0.8109778843936724 1107 24 0.9218589530440227 1107 25 0.7670824601961357 1108 1 0.745341769475246 1108 2 0.6114018065107061 1108 3 0.6033299755786068 1108 4 0.5827113882328376 1108 5 0.6835842832946213 1108 6 0.6813872373335741 1108 7 0.6891528834914311 1108 8 0.6152528089570026 1108 9 0.8265272638596457 1108 10 0.8142756713708934 1108 11 0.7458529959405737 1108 12 0.9446585348552219 1108 13 0.9021653757337731 1108 14 1.0245061182414403 1108 15 0.9386815732716158 1108 16 0.8625013023660841 1108 17 0.93963696117914 1108 18 1.0885921372653087 1108 19 1.114445623114534 1108 20 1.0692324565013251 1108 21 1.1328024787319038 1108 22 1.1062767790492813 1108 23 1.272532494368562 1108 24 1.2785967535731544 1108 25 1.2590844087741289 1109 1 0.3025568511275587 1109 2 0.1573785804572374 1109 3 0.3186764873469059 1109 4 0.2574065959082148 1109 5 0.12285913399166049 1109 6 0.3392480974687473 1109 7 0.35477298365943 1109 8 0.3102826423565972 1109 9 0.5554506781428046 1109 10 0.16307545556753666 1109 11 0.44859856063235526 1109 12 0.6471071523921808 1109 13 0.3915530500338704 1109 14 0.6662744548642223 1109 15 0.46341275723690245 1109 16 0.5547449447873234 1109 17 0.6767952204161816 1109 18 0.8066084541307674 1109 19 0.8031778382369765 1109 20 0.9018127260488455 1109 21 0.88633987531027 1109 22 0.8953844746033965 1109 23 0.7803453045382938 1109 24 0.9302383111643167 1109 25 0.8293706038314284 1110 1 0.5041991766093403 1110 2 0.35077358397033365 1110 3 0.3283628636727691 1110 4 0.28349180445837874 1110 5 0.4489007576056778 1110 6 0.3779165776695379 1110 7 0.24553072280358634 1110 8 0.36472811797721505 1110 9 0.28519589413246393 1110 10 0.41825586338728016 1110 11 0.2921643344340552 1110 12 0.485837252675104 1110 13 0.3915831086105186 1110 14 0.4784928905407426 1110 15 0.421392745739503 1110 16 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11 1.037455015823177 1203 12 1.0969765043078397 1203 13 1.03608236912212 1203 14 1.143257081777982 1203 15 1.1601952056754143 1203 16 1.1089697912335907 1203 17 0.9904076917646023 1203 18 1.1030572339205307 1203 19 1.148867587153589 1203 20 1.1552061265531535 1203 21 1.0900651415396396 1203 22 1.1083756328952674 1203 23 1.2311568615346715 1203 24 1.2199005738704543 1203 25 1.260811094431098 1204 1 0.5698939255098416 1204 2 0.7323576478599172 1204 3 0.5870566628363179 1204 4 0.45042010512617736 1204 5 0.6674146543537276 1204 6 0.5981302657188365 1204 7 0.5531984797041147 1204 8 0.7231296393263804 1204 9 0.8943210575154268 1204 10 0.8506162833070832 1204 11 0.8728905928078632 1204 12 0.9824467968400867 1204 13 0.971435962993219 1204 14 1.0833505987043353 1204 15 1.0355993060599644 1204 16 0.9396224347501203 1204 17 1.0353684952342235 1204 18 1.0109416570411724 1204 19 1.1258004004998057 1204 20 1.0292474990593234 1204 21 0.8922571803999801 1204 22 1.0085822584299322 1204 23 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2216 19 -3.744944961675113 2216 20 -3.026413597899648 2216 21 -3.9533669290944253 2216 22 -3.2358372398646083 2216 23 -4.574090374060884 2216 24 -3.7424378557413647 2216 25 -3.834197226684349 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41823.0 3 0.0 4 696.0 5 5918.0 6 7201.0 7 5756.0 8 4526.0 9 3769.0 10 3355.0 11 3017.0 12 2734.0 13 2614.0 14 2501.0 15 2645.0 16 2695.0 17 2681.0 18 3056.0 19 3695.0 20 5026.0 21 6586.0 22 8558.0 23 10270.0 24 12209.0 25 14288.0 26 15966.0 27 17346.0 28 20020.0 29 23753.0 30 29679.0 31 38180.0 32 48315.0 33 61799.0 34 80618.0 35 104815.0 36 143199.0 37 220870.0 38 693903.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.140668234622126 19.8068837157519 13.519168527781794 26.53327952184418 2 13.19652811756644 20.645737230683253 41.44884743349601 24.708887218254297 3 17.297967353084346 25.894041136685907 29.765170778017303 27.04282073221245 4 11.405565477737968 17.213548877799152 37.66504109476403 33.71584454969885 5 12.390369442280994 39.407015388187716 35.13233067430352 13.070284495227774 6 31.393396855893403 37.17842233287217 18.321707426404714 13.10647338482972 7 28.0298052313445 31.88361075311757 22.030035153957392 18.056548861580538 8 25.22877643701003 37.68563603993387 20.298566332311573 16.78702119074453 9 27.37756657694394 14.32209350071631 19.9861757255174 38.314164196822354 10 15.757796087393364 29.148985977840137 33.80495279067052 21.288265144095973 11 34.157332038668244 22.032301732743157 25.218338697204963 18.592027531383636 12 24.179506742778102 24.77549263451398 31.498440811914396 19.546559810793525 13 29.483420133325755 22.314712145267755 25.757650874186965 22.44421684721952 14 20.7451442473077 22.89106439936907 27.58071156644614 28.783079786877092 15 22.825476389867248 32.356720248874595 22.95493229413881 21.86287106711935 16 21.57578806332561 27.969476301265832 28.624220643220262 21.830514992188295 17 21.021969125811744 29.341845208261415 28.060529515363637 21.575656150563212 18 20.911326832665857 26.97964430127498 31.262273395631397 20.846755470427766 19 23.631275324081745 26.44181763880106 27.851985843301748 22.074921193815452 20 24.219593105612255 26.973756998768472 28.354254821978415 20.452395073640858 21 23.008695473432645 26.384108618754006 27.230682452289155 23.376513455524194 22 23.613418553529844 28.51841641335181 27.257404169598832 20.610760863519516 23 21.623958183451606 28.183366967296664 28.575185510754608 21.617489338497123 24 23.332156446899248 26.452134779291477 29.01597381508293 21.199734958726353 25 22.275074633542957 27.597724901183856 28.669618555791065 21.45758190948212 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 93.0 1 93.0 2 261.0 3 429.0 4 429.0 5 429.0 6 1135.5 7 1842.0 8 1842.0 9 1842.0 10 2219.0 11 2596.0 12 2596.0 13 2596.0 14 3894.0 15 5192.0 16 5192.0 17 5192.0 18 10823.5 19 16455.0 20 16455.0 21 16455.0 22 28448.5 23 40442.0 24 40442.0 25 40442.0 26 62119.0 27 83796.0 28 83796.0 29 83796.0 30 109089.5 31 134383.0 32 134383.0 33 134383.0 34 150610.5 35 166838.0 36 166838.0 37 166838.0 38 177405.5 39 187973.0 40 187973.0 41 187973.0 42 197125.0 43 206277.0 44 206277.0 45 206277.0 46 214585.5 47 222894.0 48 222894.0 49 222894.0 50 211812.5 51 200731.0 52 200731.0 53 200731.0 54 173899.5 55 147068.0 56 147068.0 57 147068.0 58 125345.0 59 103622.0 60 103622.0 61 103622.0 62 85557.0 63 67492.0 64 67492.0 65 67492.0 66 52090.0 67 36688.0 68 36688.0 69 36688.0 70 27207.5 71 17727.0 72 17727.0 73 17727.0 74 12593.0 75 7459.0 76 7459.0 77 7459.0 78 5159.0 79 2859.0 80 2859.0 81 2859.0 82 1882.5 83 906.0 84 906.0 85 906.0 86 587.5 87 269.0 88 269.0 89 269.0 90 159.5 91 50.0 92 50.0 93 50.0 94 25.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0845786363674836 2 0.04141269900766709 3 0.019769273832857138 4 0.006045649490170378 5 0.020917947235989512 6 0.061726081294639565 7 0.08173718110710353 8 0.06341886315188727 9 0.028354096108899076 10 0.05519677984525556 11 0.006952496913695936 12 9.673039184272605E-4 13 0.005320171551349933 14 7.859344337221492E-4 15 0.014328189291703798 16 0.009310300214862384 17 0.004776063097234599 18 0.005924736500366971 19 0.0014509558776408908 20 0.002478716290969855 21 1.8136948470511135E-4 22 8.46390928623853E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1654082.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.600770690921664 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14174751008225 44.43424109025224 2 13.46443867305661 15.51124088977423 3 4.360016229862819 7.534208851950752 4 1.8685728287368883 4.3052494010944145 5 0.9755416719482799 2.8095976072665594 6 0.5510552016366372 1.9044722584506926 7 0.34825515801263174 1.4041835849031405 8 0.23330564609291346 1.0750868017196185 9 0.17164890649060652 0.8898398371901592 >10 0.786058966131188 7.963333608456525 >50 0.0529837592976488 2.1036020501402692 >100 0.03880429035219223 4.478868684576802 >500 0.004837128913467528 1.9409681115232311 >1k 0.0026288744094932217 3.2766594454383595 >5k 1.0515497637972886E-4 0.36844777726309297 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6083 0.36775685848706413 No Hit GTCCTACAGTGGACATTTCTAAATT 4943 0.2988364542991218 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4645 0.2808204188184141 No Hit TATCAACGCAGAGTACTTTTTTTTT 4425 0.2675199899400392 No Hit CTGTAGGACGTGGAATATGGCAAGA 4396 0.26576675158788987 No Hit CTTTAGGACGTGAAATATGGCGAGG 4167 0.2519222142553997 No Hit GGTATCAACGCAGAGTACTTTTTTT 3616 0.21861068556456087 No Hit GTCCTACAGTGTGCATTTCTCATTT 2557 0.15458725746365656 No Hit CTGAAGGACCTGGAATATGGCGAGA 2242 0.13554346156961988 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2025 0.12242440217595017 No Hit CTGTAGGACCTGGAATATGGCGAGA 1913 0.11565327474695934 No Hit ATTTAGAAATGTCCACTGTAGGACG 1774 0.1072498219556225 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1731 0.10465019267484925 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1656 0.10011595555722147 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.0456494901703784E-5 0.0 4 0.0 0.0 0.0 2.4182597960681514E-4 0.0 5 0.0 0.0 0.0 2.4182597960681514E-4 0.0 6 0.0 0.0 0.0 2.4182597960681514E-4 0.0 7 0.0 0.0 0.0 2.4182597960681514E-4 0.0 8 6.0456494901703784E-5 0.0 0.0 2.4182597960681514E-4 0.0 9 6.0456494901703784E-5 0.0 0.0 4.231954643119265E-4 6.0456494901703784E-5 10 6.0456494901703784E-5 0.0 0.0 6.045649490170378E-4 6.0456494901703784E-5 11 6.0456494901703784E-5 0.0 0.0 6.650214439187416E-4 6.0456494901703784E-5 12 6.0456494901703784E-5 0.0 0.0 6.650214439187416E-4 6.0456494901703784E-5 13 6.0456494901703784E-5 0.0 0.0 6.650214439187416E-4 6.0456494901703784E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTACGA 35 0.0021800383 16.274778 13 GAACCGC 40 0.0052456963 14.263719 6 ACCGTGC 40 0.005277395 14.2507715 8 CCCCCGC 40 0.005307125 14.238709 19 TAGGACC 800 0.0 14.145711 4 GGTATCA 1595 0.0 12.938382 1 GCACCGT 85 3.9015413E-6 12.305953 6 TCCAACG 105 2.7471106E-7 11.75294 18 AGGACCT 1610 0.0 11.695185 5 ACCTTTT 645 0.0 11.627846 15 GGACCTG 1650 0.0 11.4109745 6 TAGAAAT 705 0.0 11.330731 4 AATGTCC 685 0.0 11.234185 8 CACCTTT 645 0.0 11.186282 14 GCGCCCC 60 0.0058841472 11.081251 9 CCAACGT 60 0.005910694 11.074553 19 TGTACTG 270 0.0 10.918569 5 ATTTAGA 805 0.0 10.868582 1 ACATCGT 70 0.0014795645 10.867595 6 AAATGTC 675 0.0 10.843201 7 >>END_MODULE