FastQCFastQC Report
Thu 26 May 2016
SRR1512853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038032
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66830.3279143801471223No Hit
GTCCTACAGTGGACATTTCTAAATT52970.2599075971329204No Hit
GTCCTAAAGTGTGTATTTCTCATTT49220.24150749350353673No Hit
CTGTAGGACGTGGAATATGGCAAGA43310.21250893018362813No Hit
CTTTAGGACGTGAAATATGGCGAGG40440.19842671753927316No Hit
TATCAACGCAGAGTACTTTTTTTTT39240.19253868437787042No Hit
GGTATCAACGCAGAGTACTTTTTTT38780.1902816049993327No Hit
GTCCTACAGTGTGCATTTCTCATTT27450.13468875856708823No Hit
GTACTGGTTCACTATCGGTCAGTCA27210.1335111519348077No Hit
CTGAAGGACCTGGAATATGGCGAGA21550.10573926219019134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17650.014.0272591
GGCGAGG11300.012.440804519
TAGGACC7500.012.2847134
GGTACGA550.003070403812.09017518
TGTCGAG1251.834269E-811.39930718
TTAGGAC15500.011.0305043
GTCCTAT2350.010.9405731
CGTGCGC1658.0035534E-1110.937654510
AGGACGT26600.010.9265655
TCCAACG2200.010.79479918
GCGCCAC1855.456968E-1210.78233413
TGGCGAG23800.010.77665518
GTATCAA40400.010.7479991
TAGTACC1158.8377965E-710.7374234
GTCCTAA12600.010.7315381
GACGTGA14150.010.67373857
TGTAGGA24250.010.65397552
GCGTGCG1701.4006218E-1010.6159589
TAGGACG27800.010.5918414
GGACGTG26950.010.5739776
Produced by FastQC (version 0.11.5)