FastQCFastQC Report
Thu 26 May 2016
SRR1512853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038032
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84580.4150082039928716No Hit
TATCAACGCAGAGTACTTTTTTTTT63610.3121148244973582No Hit
GTCCTACAGTGGACATTTCTAAATT49330.24204722987666533No Hit
GGTATCAACGCAGAGTACTTTTTTT48360.2372877364045314No Hit
CTTTAGGACGTGAAATATGGCGAGG45340.2224695196150011No Hit
GTCCTAAAGTGTGTATTTCTCATTT44550.21859323111707765No Hit
CTGTAGGACGTGGAATATGGCAAGA39860.19558083484459515No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29140.1429810719360638No Hit
GTCCTACAGTGTGCATTTCTCATTT26890.13194100975843362No Hit
GTACTGGTTCACTATCGGTCAGTCA24580.12060654592273332No Hit
CTGAAGGACCTGGAATATGGCGAGA20910.10259897783744319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.013.7402411
TATTCCG604.0563694E-412.680175
TAGGACC6350.012.430564
TCCAACG2500.012.14966818
CTAATAC1657.2759576E-1211.5296953
CCAACGA2100.011.29991419
GGCGAGG10200.011.16697519
TAGAAAT5950.011.0285514
GCGCCAC1553.1832315E-1011.02315513
CGAAATC1900.010.99092313
GTCTTAT1855.456968E-1210.7958111
TGTAGGA22850.010.7400242
ATCCCGT1951.8189894E-1210.71541410
GTCCTAC25000.010.5757821
GTAGGAC22900.010.5504253
TAGGACA6350.010.4836044
ACGAAAT2001.8189894E-1210.44137712
CTGTAGG22800.010.4282841
GACGTGG12100.010.371887
CGCGCCT2200.010.35508412
Produced by FastQC (version 0.11.5)