FastQCFastQC Report
Thu 26 May 2016
SRR1512852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333587
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69950.2997531268386394No Hit
GTATCAACGCAGAGTACTTTTTTTT64780.2775983925176135No Hit
GTCCTAAAGTGTGTATTTCTCATTT60230.25810051221574337No Hit
CTGTAGGACGTGGAATATGGCAAGA59110.2533010339875908No Hit
CTTTAGGACGTGAAATATGGCGAGG47940.20543480915860432No Hit
TATCAACGCAGAGTACTTTTTTTTT36410.15602589489914026No Hit
GTCCTACAGTGTGCATTTCTCATTT34440.14758395551569323No Hit
GGTATCAACGCAGAGTACTTTTTTT34050.1459127086326758No Hit
CTGAAGGACCTGGAATATGGCGAGA26130.11197354116216794No Hit
CTGTAGGACCTGGAATATGGCGAGA25100.10755973529163472No Hit
GTCCTTCAGTGTGCATTTCTCATTT24150.10348874929454097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12350.013.6134944
GGTATCA18450.012.6959481
TGACCGT550.00307292412.08897110
CGTACAA550.00307292412.08897110
GTCGAGG1501.7644197E-1011.39866319
TGTCGAG1850.011.29621318
GTTACGG600.005881350511.0822717
AGGACCT22750.011.0643985
ACTATAC1303.2752723E-810.9600163
GTATCAA40650.010.8688561
GGACCGT1053.4738077E-610.8556366
TAGAAAT9750.010.8138844
TGTAGGA36450.010.7104472
GTCCTAA18750.010.6138131
ATGTCGA1803.45608E-1110.554543517
CGTCGGT909.5301744E-510.554316519
TATGTCG1803.6379788E-1110.554316516
AATGTCC8900.010.4592238
TATAAGG2001.8189894E-1210.4485492
GGACCTG23300.010.3955536
Produced by FastQC (version 0.11.5)