##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512852_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2333587
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.88833071147551	31.0	30.0	34.0	22.0	34.0
2	28.96979199832704	31.0	30.0	34.0	22.0	34.0
3	29.100181394565535	31.0	30.0	34.0	22.0	34.0
4	32.53564833880203	35.0	33.0	37.0	25.0	37.0
5	32.72093133875018	35.0	33.0	37.0	26.0	37.0
6	32.67950627081827	36.0	33.0	37.0	25.0	37.0
7	32.593515904913765	36.0	33.0	37.0	25.0	37.0
8	32.51847392019239	36.0	33.0	37.0	24.0	37.0
9	33.83079010981806	38.0	34.0	39.0	23.0	39.0
10	33.68490911202368	38.0	34.0	39.0	22.0	39.0
11	33.76614928005684	38.0	34.0	39.0	22.0	39.0
12	33.678413103946845	38.0	34.0	39.0	22.0	39.0
13	33.64615032565745	38.0	34.0	39.0	20.0	39.0
14	34.46999790451353	39.0	34.0	41.0	18.0	41.0
15	34.66486443402367	39.0	34.0	41.0	19.0	41.0
16	34.60892051592677	39.0	34.0	41.0	19.0	41.0
17	34.36539670472967	39.0	34.0	41.0	17.0	41.0
18	34.36367103519174	39.0	34.0	41.0	17.0	41.0
19	34.48689763869957	39.0	34.0	41.0	18.0	41.0
20	34.471274908542085	39.0	34.0	41.0	18.0	41.0
21	34.36175467209922	39.0	34.0	41.0	18.0	41.0
22	34.36732035274451	39.0	34.0	41.0	18.0	41.0
23	34.02832334941873	39.0	34.0	41.0	10.0	41.0
24	34.106772106632405	39.0	34.0	41.0	8.0	41.0
25	33.81253452303257	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7646236602668814
1101	2	0.4517020801231162
1101	3	0.7848038553603622
1101	4	0.7894804326019127
1101	5	0.63660193809595
1101	6	0.7556028303339488
1101	7	0.4420891446646209
1101	8	0.6586568489583442
1101	9	0.751311195434873
1101	10	0.8110699871361788
1101	11	0.8769954400728253
1101	12	0.8038692580862303
1101	13	1.1598835951633575
1101	14	1.2436329267444677
1101	15	1.0809636743987738
1101	16	0.8809902570758936
1101	17	0.25473317234926185
1101	18	0.7738376361146422
1101	19	0.26882733596227837
1101	20	-0.3531620434688776
1101	21	0.7022138208792867
1101	22	0.7696378691845354
1101	23	0.41542857758681606
1101	24	0.8798169900651587
1101	25	0.37804938768971397
1102	1	0.6894402165411115
1102	2	0.6328553930979197
1102	3	0.6101269089152872
1102	4	0.6961036977020143
1102	5	0.6105719234654003
1102	6	0.49782986385373107
1102	7	0.43477387055332173
1102	8	0.5006113428652981
1102	9	0.8088103112653044
1102	10	0.9282688879877625
1102	11	0.6591816316696395
1102	12	0.8081365273304542
1102	13	0.741481443314683
1102	14	0.961344817631236
1102	15	0.9804316431124391
1102	16	0.9418181581899887
1102	17	1.034195684651941
1102	18	1.0859666633782794
1102	19	0.8934443648965527
1102	20	1.0546127490985384
1102	21	1.228175318220849
1102	22	1.0346003126577727
1102	23	0.9491757615886698
1102	24	0.3757460642139705
1102	25	0.7342615646634769
1103	1	0.6919104929399609
1103	2	0.8027132100985916
1103	3	0.9413648815818298
1103	4	0.923784337525511
1103	5	0.7368151054228704
1103	6	0.469838351933646
1103	7	0.3604748266114228
1103	8	0.5328753926293501
1103	9	0.6308476036597384
1103	10	0.862650820941127
1103	11	0.6854787451096129
1103	12	0.814084311849669
1103	13	1.0100335668668095
1103	14	0.8969539982404129
1103	15	0.8686178905844031
1103	16	0.9407459635463695
1103	17	0.6143945569936733
1103	18	0.9168795150054194
1103	19	0.804614923209968
1103	20	1.0471067737354218
1103	21	0.8849765071648932
1103	22	0.8643412270415425
1103	23	0.24995225236516205
1103	24	0.5419297989691358
1103	25	0.8806196574501399
1104	1	0.6547578859738792
1104	2	0.6583656348655289
1104	3	0.6695896545920625
1104	4	0.9089565157162767
1104	5	0.6062654713394835
1104	6	0.5389969714714695
1104	7	0.6893067023516082
1104	8	0.49099365505684744
1104	9	0.7192415941246466
1104	10	0.8585716857797294
1104	11	0.5363223271447737
1104	12	0.7806006642228098
1104	13	0.8184361358747338
1104	14	0.9847920535682206
1104	15	0.8372491195176224
1104	16	0.8336063382692771
1104	17	1.041210778790763
1104	18	0.8614996297908775
1104	19	0.5065368205103837
1104	20	0.7743523781810779
1104	21	0.7413253881584865
1104	22	0.6359182365462672
1104	23	0.9367485840890666
1104	24	0.8142367437931597
1104	25	0.460244479046807
1105	1	0.6478231264942451
1105	2	0.7178985970655098
1105	3	0.8258871128842316
1105	4	0.8922956423470865
1105	5	0.7021902681093906
1105	6	1.0120433974858685
1105	7	0.9183189653565407
1105	8	1.0750432094518914
1105	9	0.6422968757321357
1105	10	0.9017630822081131
1105	11	0.941266450548639
1105	12	1.0757795810769437
1105	13	1.0716530166080247
1105	14	1.0944495338195779
1105	15	1.0982451492786751
1105	16	0.9871809343384612
1105	17	1.3554116882627767
1105	18	1.2852219285167266
1105	19	0.903810116769094
1105	20	1.1127814309587052
1105	21	1.048641418330483
1105	22	1.0508290898703194
1105	23	1.2485723679860925
1105	24	1.2988673576119538
1105	25	1.6043136251852417
1106	1	0.694168339638292
1106	2	0.7440590236405491
1106	3	0.5803320046143341
1106	4	0.6355320324123497
1106	5	0.5039278929799096
1106	6	0.7335514468391153
1106	7	0.6912550724901578
1106	8	0.6945963109491728
1106	9	0.7745905020484898
1106	10	0.566859631595868
1106	11	0.47848517148569414
1106	12	0.6492757060938672
1106	13	0.5880008761710798
1106	14	0.9426897717339386
1106	15	0.7087841381395847
1106	16	0.7941682416501195
1106	17	1.008088791882372
1106	18	0.8270681151730628
1106	19	0.854790018674251
1106	20	0.914478944732906
1106	21	1.0868462100614167
1106	22	0.9974945752766189
1106	23	1.2550775201308326
1106	24	0.983503795429975
1106	25	1.0966832692597706
1107	1	0.7314812812302627
1107	2	0.7254449769767
1107	3	0.7801787235664044
1107	4	0.811134913778524
1107	5	0.72468937207762
1107	6	0.7304525006575489
1107	7	0.4481445755616633
1107	8	0.6315124127907552
1107	9	0.5686695310548373
1107	10	0.776913604228298
1107	11	0.5179088353445778
1107	12	0.7033052535096331
1107	13	0.5966068716108452
1107	14	0.7015745944463774
1107	15	0.6621482220415587
1107	16	0.6713533372025253
1107	17	0.9765067402034191
1107	18	0.8290438141209364
1107	19	0.7467784778125477
1107	20	0.9609614150290895
1107	21	0.7608758787959715
1107	22	0.7741724459581292
1107	23	1.1126345835840752
1107	24	0.8411662264982454
1107	25	1.2056926197182207
1108	1	0.6798511059667796
1108	2	0.6792319662244068
1108	3	0.6075512845043995
1108	4	0.7976830277153013
1108	5	0.6812782614417543
1108	6	0.7817881425422186
1108	7	0.6480015574320603
1108	8	0.6220585433020744
1108	9	0.7763998178627176
1108	10	0.6404384000198036
1108	11	0.42042394692730767
1108	12	0.5234801953032928
1108	13	0.5270223399221408
1108	14	0.5388621333201584
1108	15	0.5693516516108659
1108	16	0.43913364954833156
1108	17	0.701136515471795
1108	18	0.8507568205386988
1108	19	0.7202943315677999
1108	20	0.7952155681754078
1108	21	0.7045011440712443
1108	22	0.7516469830782526
1108	23	1.1700600666750631
1108	24	1.0244407140542293
1108	25	1.0966158260325116
1109	1	0.6366114045601599
1109	2	0.6210681500145334
1109	3	0.8152777448979975
1109	4	0.8003080455702616
1109	5	0.6103787116502986
1109	6	0.701658484532949
1109	7	0.5622174122978052
1109	8	0.5038768429342042
1109	9	0.6259614081941578
1109	10	0.7663985270916029
1109	11	0.6936708957045354
1109	12	0.6008628591769636
1109	13	0.788315399578579
1109	14	0.8411785291111897
1109	15	0.6767327902469802
1109	16	0.3649367526306051
1109	17	0.8083479696498017
1109	18	0.9001480285909267
1109	19	0.7383134965835509
1109	20	0.6755786259819132
1109	21	0.6961275756547991
1109	22	0.6503269903754685
1109	23	0.832215319907931
1109	24	0.6926442960981234
1109	25	0.3332553645477532
1110	1	0.48860825358281446
1110	2	0.44529378854995016
1110	3	0.35545185608624763
1110	4	0.39529590359836675
1110	5	0.29150593823919735
1110	6	0.36276834986563955
1110	7	0.2742532986490005
1110	8	0.2863646389881751
1110	9	0.4512425193145546
1110	10	0.283783793850219
1110	11	0.3971768750395981
1110	12	0.2887408748205118
1110	13	0.39800352166606245
1110	14	0.42874238194450953
1110	15	0.4684614605569237
1110	16	0.49767571036311153
1110	17	0.6796495128269342
1110	18	0.04087180429018389
1110	19	0.39016279759325556
1110	20	0.3302340448512595
1110	21	0.48151037818028186
1110	22	0.48174934840406536
1110	23	0.5974753697393851
1110	24	0.35509591909085003
1110	25	0.5244459152420973
1111	1	0.5557352792647485
1111	2	0.5513136374490202
1111	3	0.6230101807271282
1111	4	0.5012202349614086
1111	5	0.521772370380134
1111	6	0.3839943348396275
1111	7	0.3363829462695449
1111	8	0.5383561618601149
1111	9	0.6223433301554664
1111	10	0.6145418465821493
1111	11	0.37648301861388234
1111	12	-0.19542423515888174
1111	13	0.3746489514821931
1111	14	0.45605656234297953
1111	15	0.3867482324647469
1111	16	0.565169040469442
1111	17	0.005591138190254696
1111	18	0.3030547286806353
1111	19	0.31523458132962645
1111	20	0.5399486540772998
1111	21	0.43524360118198757
1111	22	0.2245869535372691
1111	23	0.3929437053566147
1111	24	0.5355622776016133
1111	25	0.6813675206980037
1112	1	0.18167120733101783
1112	2	0.11175282311219092
1112	3	0.15377115831250165
1112	4	0.2571225376847863
1112	5	0.14894117096279302
1112	6	0.09460644030340859
1112	7	-0.1125684881394804
1112	8	0.0395503918919502
1112	9	-0.0917954515271191
1112	10	-0.016394986214585572
1112	11	0.012018874301716664
1112	12	-0.0674438714969412
1112	13	-0.015375246512540741
1112	14	-0.03406287342286163
1112	15	-0.24662456583538273
1112	16	0.12347920249277422
1112	17	0.2827514870588601
1112	18	0.060692894352378346
1112	19	0.17165825270883062
1112	20	-0.041055778251127606
1112	21	-0.034513655947513655
1112	22	-0.23367568886560264
1112	23	0.084437991090347
1112	24	0.08625979926409144
1112	25	0.13980394792590545
1113	1	-0.1282928599393074
1113	2	0.09372057741177997
1113	3	0.03827812771023176
1113	4	0.05117946607760615
1113	5	-0.04017031030409157
1113	6	-0.47354422211537184
1113	7	-0.4884708206596642
1113	8	-0.2450989721278347
1113	9	-0.39913247452399503
1113	10	-0.19781076378297513
1113	11	-0.2940837266991352
1113	12	-0.43596079414943034
1113	13	-0.2804332802238534
1113	14	-0.03830460948774572
1113	15	-0.37429370165736486
1113	16	-0.2759154407537352
1113	17	-0.28644967695611
1113	18	-0.3575927448051388
1113	19	-0.7605346783537072
1113	20	-0.5065355356917323
1113	21	-0.34379736142629724
1113	22	-0.7872446247100697
1113	23	-4.213152543906077
1113	24	-2.043344595858713
1113	25	-1.1942525422190826
1114	1	-0.4693780895008821
1114	2	-0.7021087657014888
1114	3	-0.5361977507121054
1114	4	-0.3551650511580249
1114	5	-0.41088621830632377
1114	6	-1.1465443764017422
1114	7	-0.8728194109016094
1114	8	-0.7070567623006738
1114	9	-0.8748751556363814
1114	10	-0.5536379984440813
1114	11	-0.7000079386170484
1114	12	-1.4847167496610894
1114	13	-0.7660440561073187
1114	14	-2.5123757651311465
1114	15	-1.412218523025686
1114	16	-1.4567375293295015
1114	17	-1.3005406782131033
1114	18	-1.2608301807580133
1114	19	-1.3469230036848003
1114	20	-0.9356033959167434
1114	21	-0.7804335645502647
1114	22	-0.984107340234857
1114	23	-5.270343770128758
1114	24	-2.483394182959909
1114	25	-2.3353353863055872
1115	1	-1.5503096614576144
1115	2	-1.3408406911972435
1115	3	-1.1165645366701682
1115	4	-1.019423547779148
1115	5	-0.9814672215691367
1115	6	-1.094439977264038
1115	7	-1.626439289738176
1115	8	-1.8630516820880487
1115	9	-3.244850631796563
1115	10	-3.9343191266739836
1115	11	-2.565910833054158
1115	12	-2.780360132594776
1115	13	-2.222780053204879
1115	14	-2.8807920728927527
1115	15	-2.5921168764406914
1115	16	-2.6872261897483654
1115	17	-2.440955594646802
1115	18	-5.075443341349697
1115	19	-3.080736606980274
1115	20	-3.2603856442408663
1115	21	-2.927202980014595
1115	22	-2.4471717313262538
1115	23	-2.893932500874431
1115	24	-2.6665675817863104
1115	25	-2.9394451343888477
1116	1	-1.183867137822233
1116	2	-0.9769558414875412
1116	3	-1.2852798960979364
1116	4	-1.0557643246326798
1116	5	-1.181740548143079
1116	6	-3.513751850294458
1116	7	-1.91437938293479
1116	8	-2.1638099776865722
1116	9	-3.1725616060498254
1116	10	-2.5637881349215306
1116	11	-2.1237254105308843
1116	12	-1.9800399653237122
1116	13	-3.3800414110808568
1116	14	-6.4400480273076255
1116	15	-4.342257832242218
1116	16	-3.1724522788006553
1116	17	-4.136024764121032
1116	18	-2.717423631128071
1116	19	-2.5473857955328896
1116	20	-3.2072767409171306
1116	21	-3.8855133814299627
1116	22	-3.7007361194905215
1116	23	-2.5161064231877823
1116	24	-3.483969119649572
1116	25	-3.3224292824041513
1201	1	0.5663974536066902
1201	2	0.5771521189770255
1201	3	0.9426008504596481
1201	4	0.7145994496207209
1201	5	0.3158688311663269
1201	6	0.8366384103646212
1201	7	0.9078604249138493
1201	8	0.9025142738299508
1201	9	1.4039299144510196
1201	10	1.214426507505074
1201	11	1.3999330305602982
1201	12	1.2222538846884916
1201	13	1.394925623329577
1201	14	1.4641393166892271
1201	15	1.5099736429600839
1201	16	1.4696537656191282
1201	17	1.6441757483704933
1201	18	1.4138501765055551
1201	19	1.5614784100014916
1201	20	1.5726003617047937
1201	21	1.6721947314783279
1201	22	1.4744103447338262
1201	23	1.9741958787759586
1201	24	1.7599505265885327
1201	25	1.945151287059268
1202	1	1.0062556163457792
1202	2	0.9540307621675908
1202	3	0.9286049953537372
1202	4	1.0353491599862892
1202	5	0.9691510995507713
1202	6	1.1974888244540622
1202	7	1.279216224904843
1202	8	1.1771760165397964
1202	9	1.4580264732808033
1202	10	1.396684218500532
1202	11	1.3088175835351592
1202	12	1.4364487489539925
1202	13	1.4657767543151934
1202	14	1.8691308034519025
1202	15	1.571834240708263
1202	16	1.3547532119177816
1202	17	1.612060856654189
1202	18	1.5858184084818845
1202	19	1.6987070417529537
1202	20	1.4125829642116088
1202	21	1.6278726444251959
1202	22	1.5975756980939337
1202	23	1.688323662586285
1202	24	1.6349530121079567
1202	25	1.951233933989272
1203	1	1.0204841218816583
1203	2	1.1177367312980238
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2211	16	0.07479410559286492
2211	17	0.5059202754633745
2211	18	0.012944863714920984
2211	19	3.443758916432671E-4
2211	20	0.11200243545975042
2211	21	0.1820705946626049
2211	22	0.18885751845651555
2211	23	0.4534979232419687
2211	24	0.07631017605132229
2211	25	0.4515742611477407
2212	1	0.17328763519471124
2212	2	0.2120017857439045
2212	3	-0.10408344828775284
2212	4	0.1960518897076753
2212	5	0.024630833864783597
2212	6	-0.06851629099879375
2212	7	0.043267137084001206
2212	8	-0.02510426159793866
2212	9	0.16782924577895386
2212	10	-0.05823800770861709
2212	11	-0.23993826040226907
2212	12	0.2502413553122125
2212	13	0.2999752854897366
2212	14	0.2589534862076448
2212	15	-0.30661342774938305
2212	16	-0.1449973875575168
2212	17	0.0354040145401342
2212	18	0.25448184626050363
2212	19	0.09259691170429107
2212	20	0.22364287204571554
2212	21	0.37724104738958886
2212	22	0.4825824697751955
2212	23	0.4349222833883246
2212	24	0.32757300248709953
2212	25	0.4424062000241591
2213	1	-0.18128051932436406
2213	2	0.002286704515579885
2213	3	-0.3303887336478972
2213	4	-0.07365937472050632
2213	5	-0.06850980762857262
2213	6	0.05944342240774603
2213	7	-0.1984831635039015
2213	8	-0.020281755457787654
2213	9	-0.43502600058701546
2213	10	-0.09251849994483763
2213	11	-0.20615771837199048
2213	12	-0.11955105333429827
2213	13	-0.2461690690475251
2213	14	-0.13719665158568972
2213	15	-0.10527282880055111
2213	16	-0.0729269268069146
2213	17	-0.16651245380625568
2213	18	-0.06668171508793819
2213	19	-0.1306276175185701
2213	20	-0.0057899862865440355
2213	21	-0.2086487902907166
2213	22	-0.3086713118565001
2213	23	-0.1264032299705491
2213	24	0.045832416578505786
2213	25	0.11467146048876486
2214	1	-2.683081570552101
2214	2	-1.3774137740283905
2214	3	-1.4391748009578507
2214	4	-3.223215662474491
2214	5	-1.746264259656499
2214	6	-1.5751695845742901
2214	7	-1.2525013638647664
2214	8	-1.1343230200690648
2214	9	-0.9011444598323237
2214	10	-1.073603147312845
2214	11	-0.989187921557054
2214	12	-1.0026775929122351
2214	13	-1.4172521474189068
2214	14	-1.2662999700135558
2214	15	-1.6916202046536952
2214	16	-1.444317528517125
2214	17	-3.4512086176095025
2214	18	-3.3618347707088674
2214	19	-2.097512060917019
2214	20	-1.9519884643598147
2214	21	-1.8771980960097068
2214	22	-2.8263175031171883
2214	23	-1.8773493349364259
2214	24	-3.0529908359514977
2214	25	-2.778221612391132
2215	1	-1.7480392584859104
2215	2	-3.3023976570133726
2215	3	-1.98692712439615
2215	4	-3.5267056432595574
2215	5	-2.0629229004941116
2215	6	-1.832328284233128
2215	7	-2.0772607850639346
2215	8	-1.5463550188142143
2215	9	-3.992364568847659
2215	10	-2.2883971553909603
2215	11	-1.7229145205139602
2215	12	-1.729638810119404
2215	13	-1.90479898871925
2215	14	-1.5490363332934223
2215	15	-1.7235950291703759
2215	16	-1.8372774908715428
2215	17	-2.131424060141171
2215	18	-1.7848484564466816
2215	19	-1.988253286751302
2215	20	-1.7445873428351675
2215	21	-2.3398923432941316
2215	22	-2.27409649217644
2215	23	-2.283657714999954
2215	24	-2.1071024394180284
2215	25	-3.1823130597853506
2216	1	-2.9934413995784155
2216	2	-3.177775093192345
2216	3	-4.384127234195763
2216	4	-5.026019395661422
2216	5	-4.529571267224828
2216	6	-3.723352165649544
2216	7	-5.241136711498193
2216	8	-3.1480569128575837
2216	9	-3.8861334406566073
2216	10	-2.8335522473481376
2216	11	-3.1450940428856313
2216	12	-3.411219924585506
2216	13	-3.1414171605556476
2216	14	-4.14854223502277
2216	15	-3.5580721085006033
2216	16	-3.862531402482851
2216	17	-3.4325495062021076
2216	18	-3.127697756474131
2216	19	-3.851552765203124
2216	20	-4.433579931361109
2216	21	-4.144449027345736
2216	22	-3.7613322868511574
2216	23	-3.798068914560755
2216	24	-4.13153285391158
2216	25	-4.326425055393365
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	114383.0
3	2.0
4	1064.0
5	6351.0
6	7358.0
7	6602.0
8	5936.0
9	5573.0
10	4952.0
11	4892.0
12	4494.0
13	4342.0
14	4343.0
15	4372.0
16	4556.0
17	4823.0
18	5650.0
19	6895.0
20	8810.0
21	11419.0
22	14467.0
23	16937.0
24	20196.0
25	22556.0
26	25505.0
27	28356.0
28	31908.0
29	37660.0
30	45780.0
31	56854.0
32	71648.0
33	90584.0
34	117746.0
35	154551.0
36	209622.0
37	327428.0
38	844972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49795677583354	18.398132892125332	12.532462841870274	24.571447490170854
2	14.627560980313586	19.904300068357095	42.53534104618497	22.932797905144355
3	19.495214385172886	25.70042089753474	28.519504385760026	26.284860331532343
4	12.176833313618902	16.357364368992457	37.89876724798527	33.567035069403374
5	12.905394980456697	39.13945175889735	34.64226325173147	13.31289000891449
6	31.57994727662361	36.17838290105511	18.834390531234977	13.407279291086303
7	28.807119987031488	31.411414862839983	21.64660635851586	18.13485879161267
8	25.876396030973915	36.82566277691129	20.29072790071601	17.007213291398784
9	28.198800704378176	14.586812551518161	19.730008380144632	37.48437836395903
10	16.396386581869777	28.612260093363506	33.41549676350142	21.575856561265297
11	34.617647790445034	22.03790798431666	24.84819736786802	18.496246857370288
12	24.755276429759554	24.347325176309916	31.133144548825904	19.76425384510463
13	29.745214161373372	22.059266902629517	25.503458172347234	22.692060763649884
14	21.329547696451396	22.49114349679622	27.289738465101514	28.889570341650874
15	23.179704035047465	31.67163801440583	22.991772079967575	22.156885870579128
16	21.995815834980238	27.676990694993414	28.39377985013175	21.933413619894598
17	21.670530657064237	28.649476377471473	27.70273081534129	21.977262150122996
18	21.39151986417919	26.862613975765527	30.60180405240494	21.144062107650345
19	24.018592537850537	26.249928693148068	27.4925336900749	22.2389450789265
20	24.537817216536542	26.70081076873125	28.042012600520437	20.719359414211763
21	23.69937982282	26.22134505670197	26.62842076326266	23.450854357215363
22	24.071546470289487	28.20975972163123	26.75275898041261	20.965934827666672
23	22.46462438649458	27.77155616670791	27.941963020040067	21.821856426757435
24	23.6810931087962	26.30792708033104	28.496340634907565	21.514639175965193
25	22.865072495966253	27.238333866591642	28.195539673131243	21.701053964310866
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	293.0
1	293.0
2	666.0
3	1039.0
4	1039.0
5	1039.0
6	1810.0
7	2581.0
8	2581.0
9	2581.0
10	3030.5
11	3480.0
12	3480.0
13	3480.0
14	5309.5
15	7139.0
16	7139.0
17	7139.0
18	14012.0
19	20885.0
20	20885.0
21	20885.0
22	35635.0
23	50385.0
24	50385.0
25	50385.0
26	78407.5
27	106430.0
28	106430.0
29	106430.0
30	141104.5
31	175779.0
32	175779.0
33	175779.0
34	198212.5
35	220646.0
36	220646.0
37	220646.0
38	237425.0
39	254204.0
40	254204.0
41	254204.0
42	269945.5
43	285687.0
44	285687.0
45	285687.0
46	302160.5
47	318634.0
48	318634.0
49	318634.0
50	306615.5
51	294597.0
52	294597.0
53	294597.0
54	259102.0
55	223607.0
56	223607.0
57	223607.0
58	192676.5
59	161746.0
60	161746.0
61	161746.0
62	133518.0
63	105290.0
64	105290.0
65	105290.0
66	80888.5
67	56487.0
68	56487.0
69	56487.0
70	41867.0
71	27247.0
72	27247.0
73	27247.0
74	19235.0
75	11223.0
76	11223.0
77	11223.0
78	7694.0
79	4165.0
80	4165.0
81	4165.0
82	2807.0
83	1449.0
84	1449.0
85	1449.0
86	933.5
87	418.0
88	418.0
89	418.0
90	272.5
91	127.0
92	127.0
93	127.0
94	82.0
95	37.0
96	37.0
97	37.0
98	24.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33643485329666306
2	0.010798826013343407
3	0.010284596203184197
4	0.011613023212762155
5	0.013155712643239784
6	0.07687735661880188
7	0.07649168426118247
8	0.10006055056014625
9	0.08077693267917588
10	0.08806185498976468
11	0.0831766717932522
12	0.09346126799643639
13	0.07987703051139726
14	0.08429083638193048
15	0.08107690006843542
16	0.08309096682489232
17	0.09564674468961303
18	0.09770366393024987
19	0.0905901515563808
20	0.08981880684114198
21	0.07859145598599923
22	0.09817504125622914
23	0.0903330366513012
24	0.087204805306166
25	0.0860906407174877
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2333587.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.84644562875414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.59601880974665	41.46172935399044
2	13.599906164663455	14.918130280327446
3	4.736522627842101	7.793442923319163
4	2.1152057696219067	4.640460729487797
5	1.1599511537115221	3.1809598942024815
6	0.7035628086042379	2.3152751597115535
7	0.4720358910084741	1.8122643581711753
8	0.2960219582145215	1.2988601788904066
9	0.2180546844048124	1.0763571953073319
>10	0.9946960840441981	9.392817308569649
>50	0.06010688033930447	2.2934297312040988
>100	0.040067702850137825	4.265639736096364
>500	0.004945162917623006	1.9166969557381504
>1k	0.0025903234330402433	2.5401260929100906
>5k	3.1397859794427186E-4	1.093810102073941
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	6995	0.2997531268386394	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6478	0.2775983925176135	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6023	0.25810051221574337	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5911	0.2533010339875908	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4794	0.20543480915860432	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3641	0.15602589489914026	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3444	0.14758395551569323	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3405	0.1459127086326758	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2613	0.11197354116216794	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2510	0.10755973529163472	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2415	0.10348874929454097	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.57049683598683E-5	0.0
8	0.0	0.0	0.0	8.57049683598683E-5	0.0
9	0.0	0.0	0.0	8.57049683598683E-5	0.0
10	0.0	0.0	0.0	8.57049683598683E-5	0.0
11	0.0	0.0	0.0	8.57049683598683E-5	0.0
12	0.0	0.0	0.0	8.57049683598683E-5	0.0
13	0.0	0.0	0.0	1.714099367197366E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1235	0.0	13.613494	4
GGTATCA	1845	0.0	12.695948	1
TGACCGT	55	0.003072924	12.088971	10
CGTACAA	55	0.003072924	12.088971	10
GTCGAGG	150	1.7644197E-10	11.398663	19
TGTCGAG	185	0.0	11.296213	18
GTTACGG	60	0.0058813505	11.08227	17
AGGACCT	2275	0.0	11.064398	5
ACTATAC	130	3.2752723E-8	10.960016	3
GTATCAA	4065	0.0	10.868856	1
GGACCGT	105	3.4738077E-6	10.855636	6
TAGAAAT	975	0.0	10.813884	4
TGTAGGA	3645	0.0	10.710447	2
GTCCTAA	1875	0.0	10.613813	1
ATGTCGA	180	3.45608E-11	10.5545435	17
CGTCGGT	90	9.5301744E-5	10.5543165	19
TATGTCG	180	3.6379788E-11	10.5543165	16
AATGTCC	890	0.0	10.459223	8
TATAAGG	200	1.8189894E-12	10.448549	2
GGACCTG	2330	0.0	10.395553	6
>>END_MODULE