FastQCFastQC Report
Thu 26 May 2016
SRR1512852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333587
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79470.3405486917779367No Hit
GTCCTACAGTGGACATTTCTAAATT66390.28449764247058285No Hit
CTGTAGGACGTGGAATATGGCAAGA58040.2487158181803378No Hit
TATCAACGCAGAGTACTTTTTTTTT57820.24777306352837927No Hit
GTCCTAAAGTGTGTATTTCTCATTT55190.23650286018905659No Hit
CTTTAGGACGTGAAATATGGCGAGG52870.22656108385931184No Hit
GGTATCAACGCAGAGTACTTTTTTT44560.1909506695057866No Hit
GTCCTACAGTGTGCATTTCTCATTT32750.14034188568928435No Hit
CTGAAGGACCTGGAATATGGCGAGA27230.1166873144219607No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26770.11471610014968373No Hit
CTGTAGGACCTGGAATATGGCGAGA25220.10807396510179393No Hit
TTTCTAAATTTTCCACCTTTTTCAG23510.1007461903070252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.013.4516941
GTGTCGT604.0575152E-412.6798926
GTCGAGG1457.2759576E-1212.43750919
TAGGACC10500.012.4084464
TCCAACG1551.8189894E-1212.24824818
CACCTTT8900.012.15931714
CGAAGGT658.080181E-411.68294517
CGCGCCT1501.7826096E-1011.39135912
TTAGGAC19250.011.3145543
CCACCTT9650.011.31266213
GAGCGTC600.00584660711.0910957
GGCGAGG14900.011.08436419
GTTCGCA600.00588500811.0813459
TCGTCGG600.00588500811.0813459
GCCTCGA2350.010.90638716
AGGACGT33400.010.8768475
GGACGTG33500.010.8725346
ACGCTGT700.001478955810.8684786
GGACCGT700.001478955810.8684786
GACGTGA17650.010.7185497
Produced by FastQC (version 0.11.5)