##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512852_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2333587
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.312937122121436	31.0	30.0	34.0	26.0	34.0
2	30.318573937890466	31.0	30.0	34.0	26.0	34.0
3	30.49244360720213	31.0	31.0	34.0	27.0	34.0
4	34.099627311945085	37.0	35.0	37.0	30.0	37.0
5	33.70331896775222	37.0	35.0	37.0	28.0	37.0
6	33.595121158971146	37.0	35.0	37.0	28.0	37.0
7	33.43655925405824	37.0	35.0	37.0	28.0	37.0
8	33.38653497812595	37.0	35.0	37.0	27.0	37.0
9	34.71383325327061	39.0	35.0	39.0	27.0	39.0
10	34.62556056405868	39.0	35.0	39.0	27.0	39.0
11	34.6263121966312	39.0	35.0	39.0	27.0	39.0
12	34.48697905841951	39.0	35.0	39.0	27.0	39.0
13	34.48668294775382	39.0	35.0	39.0	27.0	39.0
14	35.56519855484282	40.0	36.0	41.0	27.0	41.0
15	35.57685057381619	40.0	36.0	41.0	27.0	41.0
16	35.578070584040795	40.0	36.0	41.0	27.0	41.0
17	35.572453051889646	40.0	36.0	41.0	27.0	41.0
18	35.489051404554445	40.0	36.0	41.0	26.0	41.0
19	35.50270249191481	40.0	36.0	41.0	26.0	41.0
20	35.484526182225046	40.0	36.0	41.0	26.0	41.0
21	35.448066003110235	40.0	36.0	41.0	26.0	41.0
22	35.41557053583175	40.0	36.0	41.0	26.0	41.0
23	35.25666238284666	40.0	36.0	41.0	25.0	41.0
24	35.21688756408054	40.0	36.0	41.0	25.0	41.0
25	35.159385101134006	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5573235997082158
1101	2	0.4340438186021238
1101	3	0.4185308247638524
1101	4	0.3082531532185371
1101	5	0.49748123225710117
1101	6	0.463198764673443
1101	7	0.569005117804835
1101	8	0.41932491119052173
1101	9	0.7426964092560056
1101	10	0.4224990773946189
1101	11	0.7209235261451212
1101	12	0.6821374748388749
1101	13	0.8471207625390633
1101	14	0.8916084189708684
1101	15	0.6264866202881763
1101	16	0.8809176428229861
1101	17	0.9352700928969
1101	18	0.8551708889438174
1101	19	0.6572812436528963
1101	20	0.788618337646696
1101	21	0.8389836821422989
1101	22	0.7399363847904752
1101	23	0.9840575719604985
1101	24	0.9707905255131308
1101	25	0.9982872629389021
1102	1	0.6960125814726261
1102	2	0.7629063598100458
1102	3	0.44483303564040355
1102	4	0.5476416356339442
1102	5	0.6199202229958729
1102	6	0.4876263487663195
1102	7	0.5726017292112928
1102	8	0.7045102559198924
1102	9	0.8871043215513765
1102	10	0.7798332835039901
1102	11	0.9484312042955381
1102	12	0.8506956244787602
1102	13	0.9265541510920272
1102	14	0.8157811624499445
1102	15	0.9894253533457515
1102	16	0.792053684446536
1102	17	0.8862561955929209
1102	18	0.3462480337924916
1102	19	0.65286710705346
1102	20	0.8053443599511851
1102	21	0.7833663725836857
1102	22	0.6146071818478802
1102	23	0.7395120319989985
1102	24	0.7105821517951725
1102	25	0.8248420669373218
1103	1	0.7810026225340962
1103	2	0.9403846033597176
1103	3	0.6892872858089412
1103	4	0.5899726401078027
1103	5	0.6959522754564986
1103	6	0.8114835222663501
1103	7	0.8797681813777487
1103	8	0.8878497178307825
1103	9	0.9477058414386121
1103	10	0.8987846838839602
1103	11	0.9833457106386163
1103	12	1.0754184595587404
1103	13	1.1610690741783785
1103	14	1.1012259439661491
1103	15	1.2069124833328857
1103	16	1.2423470900970912
1103	17	1.0319131791070504
1103	18	1.159122030237917
1103	19	1.1600458163750247
1103	20	1.0228538592463963
1103	21	1.1263686095144934
1103	22	1.0841233306497458
1103	23	1.279928162415132
1103	24	1.1693653227212053
1103	25	1.0776586079681465
1104	1	0.6520239246506421
1104	2	0.8209989940554152
1104	3	0.725372433542077
1104	4	0.5670992077796058
1104	5	0.6615183074628135
1104	6	0.45308315483141115
1104	7	0.5565467486851432
1104	8	0.5540411483977223
1104	9	0.5768423693302651
1104	10	0.6666936450451786
1104	11	0.7000989218985225
1104	12	0.639119607413349
1104	13	0.6220954626876889
1104	14	0.7711825957532241
1104	15	0.7716854176500902
1104	16	0.9149029645011453
1104	17	0.891059424286361
1104	18	0.5000896595233897
1104	19	0.8852043296300138
1104	20	0.8835274646637856
1104	21	0.8226197862172953
1104	22	0.8293304303430418
1104	23	0.8692297323644169
1104	24	1.037179995825575
1104	25	0.9563239233244971
1105	1	0.7150978584673879
1105	2	0.5961054938225026
1105	3	0.6269088845256086
1105	4	0.4402131134877507
1105	5	0.5051412642273476
1105	6	0.6855931351480962
1105	7	0.780814066543762
1105	8	0.7278009875423308
1105	9	1.0210602105175823
1105	10	0.7415066185672146
1105	11	0.9652035150515843
1105	12	1.1212349945628262
1105	13	1.218914045273145
1105	14	1.2016113218528943
1105	15	1.06456820838509
1105	16	1.0416656984084796
1105	17	0.8896148347261672
1105	18	0.8431301721308273
1105	19	1.1255851789465297
1105	20	1.1572445036363632
1105	21	0.9726738094297431
1105	22	0.9623470965022207
1105	23	1.0137884487434121
1105	24	1.1369292623077527
1105	25	0.9775223296304247
1106	1	0.12488522475073083
1106	2	0.3595740883182721
1106	3	-1.6193054309648325
1106	4	-0.35075707955317625
1106	5	0.10635415680138038
1106	6	0.13540756995192282
1106	7	0.22547601410569484
1106	8	0.25875872407088707
1106	9	0.5930038762714318
1106	10	0.42316486456758184
1106	11	0.4233010054136912
1106	12	0.4943015274548017
1106	13	0.4648429402489782
1106	14	0.5744724280864872
1106	15	0.5619942100721929
1106	16	0.604954196365135
1106	17	0.5614572789487156
1106	18	0.6487313251992646
1106	19	0.6464260901945238
1106	20	0.6353850597679553
1106	21	0.6242434814526518
1106	22	0.5700323368898452
1106	23	0.6763917928519376
1106	24	0.6367337682832783
1106	25	0.5550584868914967
1107	1	0.5234435082066895
1107	2	0.5930428868106823
1107	3	0.4099722377134043
1107	4	0.30234629626195186
1107	5	0.467103739168941
1107	6	0.39767303059000625
1107	7	0.40637573371700597
1107	8	0.3903408581267982
1107	9	0.4480142186982121
1107	10	0.4452598618225565
1107	11	0.4433832340907742
1107	12	0.6825593065166586
1107	13	0.4635658727727403
1107	14	0.659315300409169
1107	15	0.6025716006383419
1107	16	0.6243845986589847
1107	17	0.596548963376982
1107	18	0.6463802868864192
1107	19	0.6873025839976421
1107	20	0.7360848220505289
1107	21	0.6754983483393531
1107	22	0.7311865249080611
1107	23	0.902210243477704
1107	24	0.8763859308393691
1107	25	0.8247013281206179
1108	1	0.2991022839437001
1108	2	0.2880334049279263
1108	3	0.23384399970005632
1108	4	0.18102806200926125
1108	5	0.2956417524234425
1108	6	0.3763349434549852
1108	7	0.2779981555379507
1108	8	0.25170519319387097
1108	9	0.41207677612630533
1108	10	0.3298931219130097
1108	11	0.2817685922210771
1108	12	0.4758240997618941
1108	13	0.42926652584014846
1108	14	0.5309731149542998
1108	15	0.431093851950898
1108	16	0.3167645809640547
1108	17	0.33285633156182826
1108	18	0.5201542854281485
1108	19	0.4748399890561217
1108	20	0.48721744295841773
1108	21	0.5062507711468598
1108	22	0.4675497763416274
1108	23	0.560207053977102
1108	24	0.49019442367460186
1108	25	0.5697781392877559
1109	1	0.1707532588923506
1109	2	0.10694989667647548
1109	3	0.23057420938675222
1109	4	0.19663013137638075
1109	5	-0.15231830999309892
1109	6	0.11195131957884996
1109	7	0.16343508482888325
1109	8	0.27671196846713997
1109	9	0.42768482681717046
1109	10	0.04719112442145956
1109	11	0.2506872130360591
1109	12	0.49576875257952224
1109	13	0.32706564080361034
1109	14	0.5578516173219796
1109	15	0.4340990398947753
1109	16	0.39232415160185496
1109	17	0.4523319916329456
1109	18	0.5976891882323514
1109	19	0.4680186453574393
1109	20	0.5206800298496077
1109	21	0.5637090759798937
1109	22	0.578040633674668
1109	23	0.5172081008511711
1109	24	0.5469486412555042
1109	25	0.5111063377970666
1110	1	0.2432569727364644
1110	2	0.05698295190768121
1110	3	0.1598559879862549
1110	4	0.22695290540367807
1110	5	0.3271627986624992
1110	6	0.38026347145244443
1110	7	0.32321126684556134
1110	8	0.3456963307292398
1110	9	0.3370527940812309
1110	10	0.45649067224402984
1110	11	0.29666663592444564
1110	12	0.4216741096518035
1110	13	0.41120172211480366
1110	14	0.3374901615205843
1110	15	0.464178946153055
1110	16	0.30813374553742534
1110	17	0.3425148216961986
1110	18	0.23967079091031707
1110	19	0.4271410038251702
1110	20	0.40664089874535136
1110	21	0.23905443479736732
1110	22	0.26487564238716743
1110	23	0.3751424966642958
1110	24	0.3559851637800264
1110	25	0.304891982661637
1111	1	0.07746629774777247
1111	2	-0.06901710604199351
1111	3	-0.05999690222524734
1111	4	0.13487648626165338
1111	5	0.35575996776419316
1111	6	0.27273352226634984
1111	7	0.09447971983928483
1111	8	0.1793561280871927
1111	9	0.18923361921638815
1111	10	0.10825049584977364
1111	11	0.10770468499758579
1111	12	0.3417966646869459
1111	13	0.36978702289632537
1111	14	0.17807423456444127
1111	15	0.10525650042689705
1111	16	0.1758406798406753
1111	17	0.30036403380790233
1111	18	0.1809489533148394
1111	19	-0.04356529473609072
1111	20	0.22924274813528456
1111	21	0.4435267291726177
1111	22	0.23555495458136733
1111	23	0.3413491025860722
1111	24	0.42714310049898074
1111	25	0.3371030524125871
1112	1	-0.12634090163780698
1112	2	-0.552310364637215
1112	3	-0.22369504613808644
1112	4	-0.11147516687092462
1112	5	-0.18996593237172021
1112	6	-0.12857406850732644
1112	7	-0.07616683912191746
1112	8	-0.08974666007087961
1112	9	-0.16870760279935837
1112	10	-0.4236313264986009
1112	11	-0.2683665272164433
1112	12	0.09375466874377025
1112	13	-0.14740569206649212
1112	14	-0.4130662278180566
1112	15	-0.47265901476463057
1112	16	-0.6505034688504949
1112	17	-0.5009575108024009
1112	18	-0.3144270657122945
1112	19	-0.4514182133568596
1112	20	-0.31893145473761564
1112	21	-0.29640467013627614
1112	22	-0.24878540898981782
1112	23	-0.03889996488923941
1112	24	-0.1710451129334558
1112	25	-0.33054250992702805
1113	1	-0.717764104273904
1113	2	-0.7020732977077913
1113	3	-0.6162727803465273
1113	4	-2.2752273298215293
1113	5	-0.867082606516135
1113	6	-0.5987078023464889
1113	7	-0.694371797572785
1113	8	-0.6698784433479901
1113	9	-0.546250706448923
1113	10	-0.5953049267009121
1113	11	-0.7208491472774909
1113	12	-0.46303553232218064
1113	13	-0.5771567565237703
1113	14	-0.7626979020720341
1113	15	-0.7919091224406856
1113	16	-1.036101639417268
1113	17	-1.0177116893337868
1113	18	-0.8858629344290492
1113	19	-0.6800692039226774
1113	20	-0.48928829982742883
1113	21	-0.5287888855990204
1113	22	-0.6286093411289357
1113	23	-0.4990990515376552
1113	24	-0.35861468028586074
1113	25	-0.6965967671634132
1114	1	-0.9483292733367534
1114	2	-2.289679292637505
1114	3	-1.468512606776283
1114	4	-0.9980738325804097
1114	5	-0.8704564377776336
1114	6	-0.6107796913310963
1114	7	-0.6442995215256104
1114	8	-0.7212852772723721
1114	9	-0.9554949610129739
1114	10	-1.1118737055262073
1114	11	-1.0303591514597628
1114	12	-0.9344770479760882
1114	13	-1.0529776196579093
1114	14	-0.9814178022822304
1114	15	-1.210603628883966
1114	16	-1.4391187267291201
1114	17	-1.51333261118139
1114	18	-1.2498592669535071
1114	19	-1.7818464213275647
1114	20	-1.1774564588909016
1114	21	-1.3621129720101663
1114	22	-0.9927052479999929
1114	23	-0.7802787689973698
1114	24	-0.8700206754464261
1114	25	-1.5458168130030074
1115	1	-0.6836396438017189
1115	2	-0.88199959845997
1115	3	-0.43769369020429494
1115	4	-0.19557050849985558
1115	5	-0.4256537350204823
1115	6	-0.3927355292914072
1115	7	-0.30966799176810156
1115	8	-0.7027347872698542
1115	9	-1.5249671665569764
1115	10	-1.0873829477675443
1115	11	-1.5372498228601472
1115	12	-2.2155596214999846
1115	13	-2.064456153282329
1115	14	-1.5802401437096094
1115	15	-1.789327522181317
1115	16	-1.5656381594204163
1115	17	-1.5762299143586276
1115	18	-1.8916403077824882
1115	19	-2.2239312996988687
1115	20	-2.4241209132928887
1115	21	-1.6765794191593386
1115	22	-1.63904871236101
1115	23	-1.6459251598898064
1115	24	-2.3332304864875084
1115	25	-2.1980327347393285
1116	1	-1.1341286297119737
1116	2	-1.2904221038179102
1116	3	-1.3108277130476047
1116	4	-0.9463746760410388
1116	5	-1.3483926884430062
1116	6	-1.9321561752082452
1116	7	-1.597271088118152
1116	8	-1.805487209216821
1116	9	-2.970220095377357
1116	10	-1.6625407432779866
1116	11	-2.618418476365207
1116	12	-4.918122410773513
1116	13	-3.790666036410009
1116	14	-2.9513204476180235
1116	15	-4.0406384820694505
1116	16	-3.4921652265418075
1116	17	-3.337986033542826
1116	18	-3.232485176792693
1116	19	-3.3429400701289467
1116	20	-5.285842684253879
1116	21	-4.025436333476129
1116	22	-3.1974381381992885
1116	23	-3.9515622489018014
1116	24	-3.9458721891217188
1116	25	-3.1467681129309355
1201	1	0.4648977389347486
1201	2	0.6779245824475773
1201	3	0.8063103814204666
1201	4	0.6274079816214737
1201	5	0.012629244426577202
1201	6	0.5246988404076305
1201	7	0.7729614341378408
1201	8	0.730253691137051
1201	9	0.5933270549576974
1201	10	0.7095627384400416
1201	11	0.49294991766878127
1201	12	0.9417559906963007
1201	13	0.9808378105316748
1201	14	0.9931408653918652
1201	15	0.8695739373415563
1201	16	0.8323327130020317
1201	17	0.6843969568233206
1201	18	0.9107853140586855
1201	19	1.110083181445603
1201	20	1.1744329563340727
1201	21	0.7321358235871358
1201	22	0.8451992294103903
1201	23	0.881706616762088
1201	24	0.5668815450049323
1201	25	0.7272970278715789
1202	1	0.8187787946599805
1202	2	0.8794719049470174
1202	3	0.8044611549712464
1202	4	0.7633275350522979
1202	5	0.8486355491513322
1202	6	0.9300960096505193
1202	7	0.9574225533384748
1202	8	0.7795049155553428
1202	9	1.017827412224925
1202	10	1.1437878951877494
1202	11	1.0450577943160155
1202	12	1.0449828691375984
1202	13	1.1253538709487287
1202	14	1.311628962591712
1202	15	1.2344689105280366
1202	16	1.2315146742791256
1202	17	1.1361709091740266
1202	18	1.1285912475973063
1202	19	1.1992379440931629
1202	20	1.242753161934715
1202	21	1.2726139072482283
1202	22	1.1598674356134993
1202	23	1.2273003984000823
1202	24	1.260992536227029
1202	25	1.2812779767175684
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2211	8	-0.285397582187521
2211	9	-0.03418414574791484
2211	10	-0.14775933954638987
2211	11	-0.03538026410053874
2211	12	-0.12045331419194838
2211	13	0.10027463888641819
2211	14	-0.09193669560917073
2211	15	-0.05027450184010007
2211	16	-0.03100317532484098
2211	17	-0.13790468653454013
2211	18	-0.13649118594366882
2211	19	-0.1369091534217901
2211	20	-0.09287879680339017
2211	21	-0.13826300315253803
2211	22	-0.10811628128509199
2211	23	-0.3490906916943288
2211	24	6.603986867901313E-4
2211	25	-0.23267316944353666
2212	1	-0.1743894590297117
2212	2	-0.1274857168113641
2212	3	-0.07542703002492246
2212	4	-0.12617732525468028
2212	5	-0.37945494119360035
2212	6	0.0155283552533092
2212	7	-0.4416334330314555
2212	8	-0.06125271884281602
2212	9	-0.12285694281487025
2212	10	-0.15342127376791836
2212	11	8.572313759387384E-4
2212	12	0.15392415215836053
2212	13	0.09276570982188304
2212	14	0.08109304134273998
2212	15	-0.16449462789870495
2212	16	-0.16645648509643252
2212	17	-0.22123597302692133
2212	18	-0.2600745301066496
2212	19	-0.011896444400257167
2212	20	-0.1418078226306534
2212	21	-0.03557463014696083
2212	22	-0.014730167738861155
2212	23	-0.3021750874869795
2212	24	-0.3052030637731775
2212	25	-0.2900712043869049
2213	1	-0.6499451168032628
2213	2	-0.19678133110852514
2213	3	-0.29810791829137173
2213	4	-0.5523273781971554
2213	5	-0.32344823708240256
2213	6	-0.22054512499705226
2213	7	-0.6775016337421533
2213	8	-0.6728220742248681
2213	9	-0.3471989727660372
2213	10	-0.38938482005160324
2213	11	-0.28908701863101527
2213	12	-0.5737756645488901
2213	13	-0.4120812095485107
2213	14	-0.4983068219134026
2213	15	-0.820455720950477
2213	16	-1.3220520495697627
2213	17	-0.9646482340359128
2213	18	-0.5640035417921041
2213	19	-0.6476175553987247
2213	20	-0.5698145463915338
2213	21	-0.385929120670383
2213	22	-0.6067434092367634
2213	23	-0.8392060129463914
2213	24	-0.40268940911112594
2213	25	-0.4786918405033873
2214	1	-1.1648942028627296
2214	2	-0.45928999981488516
2214	3	-0.6187444602228069
2214	4	-0.7345194233842562
2214	5	-1.0148255023212798
2214	6	-1.5149053666225356
2214	7	-1.0276762630666951
2214	8	-0.8855471075660475
2214	9	-0.7167544760217055
2214	10	-1.0417232526239744
2214	11	-1.116654289361385
2214	12	-0.7233672547269734
2214	13	-1.1066769575676503
2214	14	-0.850353421113212
2214	15	-0.896968469048069
2214	16	-0.755406878156883
2214	17	-0.8840074558135882
2214	18	-0.8581954300795474
2214	19	-0.9363827550535504
2214	20	-1.1872016963091596
2214	21	-1.2097345650886808
2214	22	-1.6947496852232717
2214	23	-1.6497543772674064
2214	24	-1.3064283280724496
2214	25	-1.7260636142540804
2215	1	-1.5260507065292508
2215	2	-0.9140786747688097
2215	3	-0.939387493829333
2215	4	-1.001827921090232
2215	5	-1.422090393894159
2215	6	-1.0383777794690943
2215	7	-1.5168489229623887
2215	8	-1.2645597737359964
2215	9	-1.2342011643868673
2215	10	-1.326037044239989
2215	11	-0.9924434740992396
2215	12	-1.2262278484291826
2215	13	-1.306963377708037
2215	14	-1.5362028387831046
2215	15	-1.4185141339239564
2215	16	-1.5296376142064503
2215	17	-1.5637660535156357
2215	18	-1.35124229098696
2215	19	-1.3615476505478412
2215	20	-1.3493631017442382
2215	21	-1.1004649678179845
2215	22	-1.2792080201905307
2215	23	-1.649912018144235
2215	24	-1.7185815769646453
2215	25	-2.0212840369825997
2216	1	-1.5189137315086647
2216	2	-0.8992557266491268
2216	3	-0.9421324842218652
2216	4	-1.1945262466460917
2216	5	-2.121513945911726
2216	6	-1.9401071234163858
2216	7	-2.024946644620364
2216	8	-2.095468365555927
2216	9	-3.9780935607634795
2216	10	-2.1001243349344705
2216	11	-2.2943924172779404
2216	12	-2.6105265033838876
2216	13	-2.4955442176751
2216	14	-2.6816873295873904
2216	15	-2.5521951660403417
2216	16	-1.9427586682827567
2216	17	-2.0875102204110547
2216	18	-2.5508030925241556
2216	19	-2.683535023592036
2216	20	-1.9595744660044616
2216	21	-2.6047197029264098
2216	22	-1.9717690962267653
2216	23	-3.1824401542957617
2216	24	-2.5695285986500735
2216	25	-2.7105511245477913
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61300.0
3	2.0
4	1006.0
5	8724.0
6	10690.0
7	8111.0
8	6528.0
9	5734.0
10	4850.0
11	4285.0
12	3959.0
13	3679.0
14	3672.0
15	3618.0
16	3702.0
17	4000.0
18	4433.0
19	5211.0
20	7110.0
21	9379.0
22	11865.0
23	14255.0
24	17233.0
25	19527.0
26	22052.0
27	24265.0
28	27489.0
29	32858.0
30	41222.0
31	52497.0
32	67508.0
33	85560.0
34	112318.0
35	147165.0
36	201325.0
37	314047.0
38	982408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.4476927636356	19.365980170689728	13.557519968056257	26.628807097618417
2	13.12227146313241	20.448329984313123	41.2988853700478	25.130513182506668
3	17.612419700214133	25.576349730748195	29.39302191217642	27.41820865686125
4	11.519728741852635	17.021174472009577	37.15284369786362	34.30625308827418
5	12.597248016678375	39.20065355639174	34.83681020933263	13.365288217597247
6	31.74994383014923	36.77397252050842	18.23333384786632	13.242749801476023
7	28.493277943092615	31.59997220766365	21.641995249569177	18.264754599674554
8	25.70358789735339	37.27774495115599	19.958081023344445	17.060586128146173
9	27.480325086158118	14.48498019649195	19.744011796375357	38.290682920974575
10	15.857480295041634	29.153819801301818	33.35297076124189	21.635729142414657
11	34.443895891509946	22.069184524636025	24.758422430329492	18.728497153524536
12	24.325980523445786	24.711526306204	31.110506411835914	19.851986758514297
13	29.52954355722634	22.027559358816784	25.613044454615423	22.829852629341453
14	21.155616311558305	22.54023894821646	27.244596239222474	29.05954850100276
15	23.10981905333767	31.77622085101805	22.914424602128637	22.199535493515636
16	21.941718862834257	27.804718119266546	28.14367356734645	22.109889450552746
17	21.42999482739927	28.84447013840885	27.737696431334047	21.987838602857828
18	21.21794044906406	26.91088787283618	30.728913775466253	21.142257902633503
19	23.835260459922022	26.215092525217205	27.648759859543027	22.300887155317746
20	24.37400552896967	26.698555448469207	28.147649459380585	20.779789563180543
21	23.3511515413307	26.085290850748727	27.03255272612583	23.531004881794747
22	23.808164760784464	28.04258720027083	27.03580144542792	21.113446593516784
23	22.080556670910493	27.735884713104763	28.196548918039056	21.987009697945695
24	23.508615706206797	26.29796960644707	28.645557247276404	21.547857440069727
25	22.611251524477776	27.147660296213637	28.490786283428164	21.750301895880416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	90.0
1	90.0
2	327.5
3	565.0
4	565.0
5	565.0
6	1383.5
7	2202.0
8	2202.0
9	2202.0
10	2794.0
11	3386.0
12	3386.0
13	3386.0
14	5139.5
15	6893.0
16	6893.0
17	6893.0
18	14039.0
19	21185.0
20	21185.0
21	21185.0
22	36622.5
23	52060.0
24	52060.0
25	52060.0
26	81458.0
27	110856.0
28	110856.0
29	110856.0
30	144490.5
31	178125.0
32	178125.0
33	178125.0
34	200415.5
35	222706.0
36	222706.0
37	222706.0
38	238959.0
39	255212.0
40	255212.0
41	255212.0
42	270808.0
43	286404.0
44	286404.0
45	286404.0
46	303525.0
47	320646.0
48	320646.0
49	320646.0
50	307076.5
51	293507.0
52	293507.0
53	293507.0
54	257555.0
55	221603.0
56	221603.0
57	221603.0
58	190362.5
59	159122.0
60	159122.0
61	159122.0
62	130950.5
63	102779.0
64	102779.0
65	102779.0
66	78855.5
67	54932.0
68	54932.0
69	54932.0
70	40197.5
71	25463.0
72	25463.0
73	25463.0
74	17918.0
75	10373.0
76	10373.0
77	10373.0
78	7095.5
79	3818.0
80	3818.0
81	3818.0
82	2523.0
83	1228.0
84	1228.0
85	1228.0
86	790.5
87	353.0
88	353.0
89	353.0
90	211.0
91	69.0
92	69.0
93	69.0
94	39.5
95	10.0
96	10.0
97	10.0
98	5.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0851907385497091
2	0.04559504316744994
3	0.019497880301870038
4	0.0070278074055092005
5	0.02168335699504668
6	0.05892216574740946
7	0.0860906407174877
8	0.06295029926032326
9	0.028582606948016082
10	0.055965344338994
11	0.007499184731488477
12	5.142298101592098E-4
13	0.006470725111170057
14	0.001157017072858222
15	0.015341189336416428
16	0.010370301171544066
17	0.005913642816830913
18	0.006684987532069728
19	0.0026140015349759836
20	0.0025711490507960492
21	8.14197199418749E-4
22	9.427546519585513E-4
23	0.0
24	0.0
25	4.285248417993415E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2333587.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.406248068696016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.13783145038803	39.59423417714597
2	14.440897643038614	15.424683237175357
3	5.034729070372402	8.066579690729514
4	2.292533607128524	4.897424741125139
5	1.2145301060027875	3.2431748064042276
6	0.7020185726878017	2.2495306825077983
7	0.45323492641444724	1.6943903839441006
8	0.32316983324246595	1.380743062597301
9	0.23780455410834325	1.143022410771824
>10	1.047600129913801	9.64333279169085
>50	0.06581086978802712	2.4290977123300395
>100	0.04169973904560061	4.355823978373324
>500	0.004996523444444078	1.8770864304219952
>1k	0.0026594398978489076	2.409357264462724
>5k	4.8353452688161953E-4	1.5915186303198037
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7947	0.3405486917779367	No Hit
GTCCTACAGTGGACATTTCTAAATT	6639	0.28449764247058285	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5804	0.2487158181803378	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5782	0.24777306352837927	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5519	0.23650286018905659	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5287	0.22656108385931184	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4456	0.1909506695057866	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3275	0.14034188568928435	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2723	0.1166873144219607	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2677	0.11471610014968373	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2522	0.10807396510179393	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2351	0.1007461903070252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.285248417993415E-5	0.0
5	0.0	0.0	0.0	4.285248417993415E-5	0.0
6	0.0	0.0	0.0	4.285248417993415E-5	0.0
7	0.0	0.0	0.0	4.285248417993415E-5	0.0
8	0.0	0.0	0.0	4.285248417993415E-5	0.0
9	0.0	0.0	0.0	4.285248417993415E-5	0.0
10	0.0	0.0	0.0	4.285248417993415E-5	0.0
11	0.0	0.0	0.0	4.285248417993415E-5	0.0
12	0.0	4.285248417993415E-5	0.0	4.285248417993415E-5	0.0
13	0.0	4.285248417993415E-5	0.0	4.285248417993415E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1895	0.0	13.451694	1
GTGTCGT	60	4.0575152E-4	12.679892	6
GTCGAGG	145	7.2759576E-12	12.437509	19
TAGGACC	1050	0.0	12.408446	4
TCCAACG	155	1.8189894E-12	12.248248	18
CACCTTT	890	0.0	12.159317	14
CGAAGGT	65	8.080181E-4	11.682945	17
CGCGCCT	150	1.7826096E-10	11.391359	12
TTAGGAC	1925	0.0	11.314554	3
CCACCTT	965	0.0	11.312662	13
GAGCGTC	60	0.005846607	11.091095	7
GGCGAGG	1490	0.0	11.084364	19
GTTCGCA	60	0.005885008	11.081345	9
TCGTCGG	60	0.005885008	11.081345	9
GCCTCGA	235	0.0	10.906387	16
AGGACGT	3340	0.0	10.876847	5
GGACGTG	3350	0.0	10.872534	6
ACGCTGT	70	0.0014789558	10.868478	6
GGACCGT	70	0.0014789558	10.868478	6
GACGTGA	1765	0.0	10.718549	7
>>END_MODULE