FastQCFastQC Report
Thu 26 May 2016
SRR1512851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226784
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53152.3436397629462395No Hit
GGTATCAACGCAGAGTACTTTTTTT32981.454247213207281No Hit
TATCAACGCAGAGTACTTTTTTTTT30891.3620890362635811No Hit
GTACTGGTTCACTATCGGTCAGTCA26521.169394666290391No Hit
GGATACCACGTGTCCCGCCCTACTC16920.7460843798504303No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15450.6812649922393114No Hit
GTACATGGGAAGCAGTGGTATCAAC14120.6226188796387753No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11850.5225236348243263No Hit
GTATTTAGCCTTGGAGGATGGTCCC11520.5079723437279525No Hit
CCCATGTACTCTGCGTTGATACCAC11520.5079723437279525No Hit
ATCATTAACTGAATCCATAGGTTAA11370.5013581205023282No Hit
ATACAGGGTGACAGCCCCGTACACA11030.4863658811909129No Hit
GTACATGGGGGTTAAGCGACTAAGC11020.4859249329758713No Hit
GTTAATGATAGTGTGTCGAAACACA10770.4749012275998307No Hit
GTTCACTATCGGTCAGTCAGGAGTA10070.44403485254691694No Hit
ACTTAGATGTTTCAGTTCCCCCGGT9990.44050726682658387No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9900.4365387328912093No Hit
CCCATATTCAGACAGGATACCACGT9410.4149322703541696No Hit
ACCCTGTATCGCGCGCCTTTCCAGA9150.40346761676308734No Hit
GGTTAATGAGGCGAACCGGGGGAAC9130.4025857203330041No Hit
TATTCAGACAGGATACCACGTGTCC8870.3911210667419218No Hit
GCCTTGGAGGATGGTCCCCCCATAT8430.3717193452800903No Hit
GTGGATGCCCTGGCAGTCAGAGGCG8270.3646641738394243No Hit
CTATCGGTCAGTCAGGAGTATTTAG8230.3629003809792578No Hit
TCTAAGTACCCCGAGGAAAAGAAAT7540.33247495414138567No Hit
GTACTTAGATGTTTCAGTTCCCCCG7450.328506420206011No Hit
GTCAGGAGTATTTAGCCTTGGAGGA7270.3205693523352618No Hit
AGCGTACACGGTGGATGCCCTGGCA6740.2971990969380556No Hit
GGTATCAACGCAGAGTACATGGGGG6710.29587625229293074No Hit
CGGTACTGGTTCACTATCGGTCAGT6640.2927896147876394No Hit
TCACGGTACTGGTTCACTATCGGTC6640.2927896147876394No Hit
GAGTACATGGGAAGCAGTGGTATCA6490.28617539156201494No Hit
GTATCAACGCAGAGTACATGGGGGT6430.2835297022717652No Hit
GCCCAGAGCCTGAATCAGTGTGTGT6280.27691547904614083No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6150.2711831522505997No Hit
GGTTCACTATCGGTCAGTCAGGAGT6070.2676555665302667No Hit
TCATTAACCTATGGATTCAGTTAAT5910.2606003950896007No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5720.2522223790038098No Hit
GAGTACATGGGGGTTAAGCGACTAA5710.2517814307887682No Hit
CATCTAAGTACCCCGAGGAAAAGAA5680.25045858614364325No Hit
GGGTACTTAGATGTTTCAGTTCCCC5280.23282065754197828No Hit
GTTATAACCGGCGATTTCCGAATGG5090.2244426414561874No Hit
GTGCTAATCTGCGATAAGCGTCGGT4960.21871031466064622No Hit
ACGCAGAGTACATGGGGGTTAAGCG4890.21562367715535485No Hit
GGAGTATTTAGCCTTGGAGGATGGT4840.21341893608014675No Hit
GTGGTATCAACGCAGAGTACATGGG4700.20724566106956396No Hit
ATCAACGCAGAGTACTTTTTTTTTT4700.20724566106956396No Hit
CCTATGGATTCAGTTAATGATAGTG4670.20592281642443913No Hit
GTCCCGCCCTACTCATCGAGCTCAC4580.2019542824890645No Hit
GTGGTATCAACGCAGAGTACTTTTT4540.200190489628898No Hit
GATATGAACCGTTATAACCGGCGAT4450.19622195569352335No Hit
GCCCTACTCATCGAGCTCACAGCAT4440.1957810074784817No Hit
CCCCAGTAGCGGCGAGCGAACGGGG4150.1829935092422746No Hit
GCCTGAATCAGTGTGTGTGTTAGTG4030.1777021306617751No Hit
GGGTTTCCCCATTCGGAAATCGCCG4020.17726118244673345No Hit
CACACACACTGATTCAGGCTCTGGG3990.17593833780160859No Hit
AGCTCACAGCATGTGCATTTTTGTG3990.17593833780160859No Hit
CATGTACTCTGCGTTGATACCACTG3980.17549738958656697No Hit
GGTGATATGAACCGTTATAACCGGC3980.17549738958656697No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3770.16623747707069284No Hit
CACTATCATTAACTGAATCCATAGG3760.1657965288556512No Hit
GTATCAACGCAGAGTACATGGGAAG3710.16359178778044306No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG3530.15565471990969382No Hit
GTGGTATCCTGTCTGAATATGGGGG3510.15477282347961055No Hit
TTCCAGACGCTTCCACTAACACACA3480.1534499788344857No Hit
GTCCCCCCATATTCAGACAGGATAC3450.1521271341893608No Hit
GGTATCAACGCAGAGTACATGGGGA3440.15168618597431918No Hit
GGTATCAACGCAGAGTACATGGGAA3440.15168618597431918No Hit
TTTGTGTACGGGGCTGTCACCCTGT3410.15036334132919432No Hit
GCGTTGATACCACTGCTTCCCATGT3370.1485995484690278No Hit
CCGTTATAACCGGCGATTTCCGAAT3360.14815860025398617No Hit
ATCCATAGGTTAATGAGGCGAACCG3320.1463948073938197No Hit
TATCAACGCAGAGTACATGGGGGTT3280.14463101453365318No Hit
AGTCAGGAGTATTTAGCCTTGGAGG3240.14286722167348664No Hit
GGGGTTAAGCGACTAAGCGTACACG3200.14110342881332016No Hit
ACGCAGAGTACATGGGAAGCAGTGG3160.13933963595315366No Hit
TATCAACGCAGAGTACATGGGAAGC3160.13933963595315366No Hit
CTATCATTAACTGAATCCATAGGTT3160.13933963595315366No Hit
GGGTGACAGCCCCGTACACAAAAAT3120.13757584309298715No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA3100.1366939466629039No Hit
TAAGTACCCCGAGGAAAAGAAATCA3050.13448920558769578No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA3040.13404825737265416No Hit
GCGTACACGGTGGATGCCCTGGCAG3040.13404825737265416No Hit
GTATCGCGCGCCTTTCCAGACGCTT3010.1327254127275293No Hit
ATGATAGTGTGTCGAAACACACTGG2910.12831593057711302No Hit
GTACATGGGCATCTAAGTACCCCGA2830.12478834485678003No Hit
CATTAACCTATGGATTCAGTTAATG2770.12214265556653027No Hit
ATTTAGCCTTGGAGGATGGTCCCCC2750.121260759136447No Hit
TCGGTAAGGTGATATGAACCGTTAT2710.11949696627628051No Hit
TAATGATAGTGTGTCGAAACACACT2700.1190560180612389No Hit
GGAGGATGGTCCCCCCATATTCAGA2660.11729222520107238No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC2660.11729222520107238No Hit
CCCTCACGGTACTGGTTCACTATCG2590.11420558769578101No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2570.11332369126569775No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2560.11288274305065614No Hit
CCCTGGCAGTCAGAGGCGATGAAGG2520.11111895019048965No Hit
GGGCTGTCACCCTGTATCGCGCGCC2430.10715041625511501No Hit
CCACGTGTCCCGCCCTACTCATCGA2390.10538662339494849No Hit
AAGTACCCCGAGGAAAAGAAATCAA2390.10538662339494849No Hit
ATTCAGACAGGATACCACGTGTCCC2360.10406377874982362No Hit
GTGCATTTTTGTGTACGGGGCTGTC2350.10362283053478198No Hit
GAATATGGGGGGACCATCCTCCAAG2340.10318188231974038No Hit
CTCCAAGGCTAAATACTCCTGACTG2340.10318188231974038No Hit
GGGAAACCCAGTGTGTTTCGACACA2330.10274093410469876No Hit
GTACACAAAAATGCACATGCTGTGA2330.10274093410469876No Hit
ACTCTGCGTTGATACCACTGCTTCC2320.10229998588965712No Hit
TCCCCCAGTAGCGGCGAGCGAACGG2320.10229998588965712No Hit
AGGTTAATGAGGCGAACCGGGGGAA2320.10229998588965712No Hit
GGTATCAACGCAGAGTACATGGGCA2310.10185903767461549No Hit
TTTCAGTTCCCCCGGTTCGCCTCAT2310.10185903767461549No Hit
GTGTTTCGACACACTATCATTAACT2310.10185903767461549No Hit
CTGTCACCCTGTATCGCGCGCCTTT2290.10097714124453225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACTC250.006027443318.994045
ACCCGCC250.006027443318.994048
AGACCCG350.002167048916.2806055
TATAGAC350.002167048916.2806052
CCGCCGT350.002167048916.28060510
GTACCGA400.00527166514.2455296
GACCCGA400.00527166514.2455296
TACCGAG400.00527166514.2455297
GAAATCG657.9685205E-411.69380216
GGTATCA11950.011.5516311
TGTCCCG3550.011.50597311
CGCCCTA4000.011.16392716
CGGTGAT600.005843860611.0847517
CCCGCCC4000.010.92639614
CCGCCCT4100.010.89163715
GTGTCCC3750.010.88991510
AAATCGC700.001481314710.85853117
AATCGCC700.001481314710.85853118
ATCGCCG700.001484048410.85613319
TCCCGCC4050.010.79150313
Produced by FastQC (version 0.11.5)