##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512851_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	226784
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.318391068152955	31.0	27.0	33.0	10.0	34.0
2	27.179823091576125	31.0	27.0	33.0	10.0	34.0
3	27.34882972343728	31.0	27.0	33.0	10.0	34.0
4	30.481096550021167	35.0	30.0	37.0	10.0	37.0
5	30.74354451813179	35.0	31.0	37.0	10.0	37.0
6	30.4311327077748	35.0	31.0	37.0	2.0	37.0
7	30.21909393960773	35.0	30.0	37.0	2.0	37.0
8	30.009572985748555	35.0	30.0	37.0	2.0	37.0
9	30.736436432905318	37.0	30.0	39.0	2.0	39.0
10	30.55707633695499	37.0	30.0	39.0	2.0	39.0
11	30.529816918301115	37.0	30.0	39.0	2.0	39.0
12	30.402797375476226	37.0	29.0	39.0	2.0	39.0
13	30.268083286298857	37.0	29.0	39.0	2.0	39.0
14	30.865823867644984	38.0	27.0	40.0	2.0	41.0
15	30.97860078312403	38.0	28.0	40.0	2.0	41.0
16	30.954286898546634	38.0	29.0	40.0	2.0	41.0
17	30.83866586708057	38.0	28.0	40.0	2.0	41.0
18	30.88101453365317	38.0	28.0	40.0	2.0	41.0
19	31.077937597008606	38.0	30.0	40.0	2.0	41.0
20	31.113729363623538	38.0	30.0	41.0	2.0	41.0
21	31.040708339212642	38.0	30.0	41.0	2.0	41.0
22	31.032881508395654	38.0	30.0	41.0	2.0	41.0
23	30.729795752786792	38.0	29.0	40.0	2.0	41.0
24	30.754096408917736	38.0	30.0	40.0	2.0	41.0
25	30.510745908000565	38.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5504362989354199
1101	2	0.337882579296199
1101	3	0.8690083150386307
1101	4	0.955652363208646
1101	5	0.7998584983626742
1101	6	0.9648755243603482
1101	7	0.6994651945312143
1101	8	1.0662364692093824
1101	9	1.2854541795334171
1101	10	0.8784987375863516
1101	11	0.7701665055142541
1101	12	0.8598156414224079
1101	13	1.2624196041505371
1101	14	1.8116676601055843
1101	15	1.2707959573512255
1101	16	1.0408013026770746
1101	17	-0.05635828309409874
1101	18	0.47930325577172894
1101	19	0.05772497095544793
1101	20	-0.5477698353788263
1101	21	0.5776121840701194
1101	22	0.5682434337149935
1101	23	0.10489395214750274
1101	24	0.6984757320525539
1101	25	0.04706911636975519
1102	1	0.7737235790869867
1102	2	0.7599742646350229
1102	3	1.030984593468613
1102	4	1.0258048837336027
1102	5	1.1276694871074717
1102	6	0.9950941399965139
1102	7	1.29713283301518
1102	8	1.2410684197937698
1102	9	1.7474538161833308
1102	10	1.3520823210833335
1102	11	1.259304933246515
1102	12	1.1975581992340025
1102	13	1.575549519109316
1102	14	1.8035355390926995
1102	15	1.6112982455916551
1102	16	1.7414008303219575
1102	17	1.74663675977256
1102	18	1.7741532008366576
1102	19	0.8665163128419273
1102	20	1.125370807058733
1102	21	1.349462933695957
1102	22	0.7396149072726246
1102	23	1.0886470125068755
1102	24	0.3467268934751573
1102	25	0.3988352573322871
1103	1	0.828069292527644
1103	2	0.7949934897163615
1103	3	1.0895217971699687
1103	4	1.544298989450425
1103	5	0.8614591520417676
1103	6	0.722807834169835
1103	7	0.5719289626471564
1103	8	0.604274571819797
1103	9	1.05150587178818
1103	10	1.3951718550335634
1103	11	0.6311306821796308
1103	12	0.9587362108113027
1103	13	1.205042061467875
1103	14	1.2395350320575886
1103	15	1.0390753191540014
1103	16	1.006233719999571
1103	17	0.049640942812239075
1103	18	0.7319932312587625
1103	19	1.3820358125672847
1103	20	1.2247412384611103
1103	21	0.29739887826011824
1103	22	0.7084575996290674
1103	23	0.41089317805383985
1103	24	0.426399010769277
1103	25	0.30274365298868844
1104	1	1.5489273294182908
1104	2	1.2098815002565466
1104	3	1.0604704137707621
1104	4	1.4205835744306583
1104	5	1.5188866882736534
1104	6	1.4433200212581099
1104	7	1.5403742854403042
1104	8	1.0049250988290197
1104	9	1.5288197716564298
1104	10	1.520822382042784
1104	11	1.9049284423904354
1104	12	1.9382651963185502
1104	13	1.952785803233354
1104	14	2.2861753482631215
1104	15	2.042047980550578
1104	16	1.6665515724369868
1104	17	2.642699243207492
1104	18	2.1578084486500657
1104	19	1.9186300154658333
1104	20	2.167338405786534
1104	21	2.112731552831921
1104	22	1.9928202478504105
1104	23	2.0709969329945537
1104	24	2.2464171267113073
1104	25	1.842919871697518
1105	1	0.9440113215131518
1105	2	0.9515152288467945
1105	3	0.9330000580395357
1105	4	1.225458409740277
1105	5	0.9136330650852464
1105	6	1.1344020370683872
1105	7	0.9835231655457086
1105	8	1.0970281950082033
1105	9	0.7094768862809318
1105	10	0.6473457680770416
1105	11	0.7267828560926723
1105	12	1.471779689072175
1105	13	0.981853655670772
1105	14	1.1612741624923686
1105	15	0.8999448843713935
1105	16	1.151161256231454
1105	17	1.6989163051310747
1105	18	0.9754714921283281
1105	19	0.6776879864803256
1105	20	0.9261905138234248
1105	21	0.39884815362243486
1105	22	0.6823706431073298
1105	23	1.271762743271232
1105	24	0.9481381412040619
1105	25	1.6215842326988295
1106	1	1.0336282694131391
1106	2	0.9962580132619543
1106	3	0.98275988067779
1106	4	0.9391259385820874
1106	5	0.8381147335994328
1106	6	0.9846298976423498
1106	7	1.062071426825831
1106	8	0.9706168964082131
1106	9	1.295583549663121
1106	10	0.7694461909534205
1106	11	0.353920845171789
1106	12	0.5687538648737913
1106	13	0.510038571711334
1106	14	0.9879523248276314
1106	15	1.0133250214982965
1106	16	1.6446210419193434
1106	17	1.2301557845415125
1106	18	1.296176012389445
1106	19	1.399862310083396
1106	20	1.4239940200023504
1106	21	1.5907929744131728
1106	22	1.0377838713374103
1106	23	1.102456178423342
1106	24	0.9456912060314195
1106	25	1.0709088621687997
1107	1	1.380979468455724
1107	2	1.570844067101465
1107	3	1.3238393977279799
1107	4	1.7414391894039198
1107	5	1.463071180252264
1107	6	1.5978988982634554
1107	7	0.9534758897183799
1107	8	1.1347713555135144
1107	9	0.9718344775387813
1107	10	1.4588237189411792
1107	11	1.4233107953316093
1107	12	1.1959523620939514
1107	13	1.2352055893296097
1107	14	1.1310951343593771
1107	15	1.0873354137056594
1107	16	0.7512775082658649
1107	17	1.3223449651326256
1107	18	1.279633314960229
1107	19	1.0315979299043327
1107	20	1.1838825234335921
1107	21	1.1545096276982356
1107	22	1.541449926994801
1107	23	1.841885970427704
1107	24	1.6677072336631795
1107	25	1.8417500856012552
1108	1	1.355541468042098
1108	2	1.2510668342595608
1108	3	1.1783759888586545
1108	4	1.6041596668465345
1108	5	1.524066246284704
1108	6	1.464932904231489
1108	7	1.6635535923212679
1108	8	1.4245163846150568
1108	9	2.0417372754233796
1108	10	2.1496678114752292
1108	11	1.8329482816671785
1108	12	1.9151298372025316
1108	13	1.6050020262368712
1108	14	1.8615143738783928
1108	15	1.7453848715601197
1108	16	1.1930301008146884
1108	17	2.100493693232604
1108	18	1.8192380066611165
1108	19	1.9980499049685925
1108	20	1.5098641466202913
1108	21	1.7441039787485444
1108	22	1.7986809962180672
1108	23	2.165060845601282
1108	24	2.2503881211864467
1108	25	2.0385031213208222
1109	1	0.6728823083630502
1109	2	0.6536516265879797
1109	3	0.8221127224157954
1109	4	1.0082724523102726
1109	5	0.6668771514889116
1109	6	0.49573363285087524
1109	7	0.7512363116974257
1109	8	0.9377263745287969
1109	9	1.174635042897446
1109	10	1.1160895977970569
1109	11	1.1049686950533015
1109	12	1.4844164224765883
1109	13	1.3987078988489117
1109	14	1.5148104733560146
1109	15	1.1589742252867694
1109	16	0.8051832915353252
1109	17	1.2818883068686269
1109	18	1.5710407374784054
1109	19	1.51025126887183
1109	20	1.3144541476740734
1109	21	1.301655877825727
1109	22	1.6442708918930222
1109	23	1.797363975352738
1109	24	1.7277693065478203
1109	25	1.513769202884042
1110	1	0.8145789867421982
1110	2	0.8329621729350727
1110	3	1.1755691220330071
1110	4	0.8172445542015581
1110	5	0.7667084040913466
1110	6	0.6034543741706138
1110	7	0.4662212267027961
1110	8	0.395470126857294
1110	9	0.8854414509155042
1110	10	0.6147856406340608
1110	11	0.6217667314472202
1110	12	0.34258825261881753
1110	13	1.0383481966040584
1110	14	0.9410620165546142
1110	15	0.9189292259807473
1110	16	0.9197123694148068
1110	17	0.9639199963149778
1110	18	0.0471348561567666
1110	19	0.7346487822218641
1110	20	1.1742769263812214
1110	21	0.9327565345526132
1110	22	1.1062662020302838
1110	23	1.3530542690900873
1110	24	1.4199258398480339
1110	25	0.9986367252193276
1111	1	0.23346553057882957
1111	2	0.49493952733584834
1111	3	0.6924820191866203
1111	4	0.2878466305235037
1111	5	0.5069186031672714
1111	6	0.41134468276681346
1111	7	0.15159285410568302
1111	8	0.3329594315176081
1111	9	0.25228447184985114
1111	10	0.29034607643350085
1111	11	-0.13532784387649244
1111	12	-0.3662560802771928
1111	13	0.11780031000317948
1111	14	-0.007828520208832401
1111	15	0.011885988287520632
1111	16	0.38252110822035235
1111	17	-0.333545921202564
1111	18	-0.12610112581802113
1111	19	-0.10796572921501735
1111	20	0.7694297984364376
1111	21	0.7274559242052305
1111	22	0.43146870046163244
1111	23	0.6973871651028674
1111	24	0.5236777650091788
1111	25	0.5783526569973212
1112	1	0.43712920107604347
1112	2	-0.10295272434238356
1112	3	0.21153993248733727
1112	4	0.6692466760349376
1112	5	-0.06099299087073007
1112	6	-0.21226152954402977
1112	7	0.04438498062746987
1112	8	0.4556269704867759
1112	9	0.30873287326070553
1112	10	-0.006416922770050348
1112	11	0.12651935148134186
1112	12	0.09434808401908512
1112	13	0.1523178887948511
1112	14	0.2701325675144588
1112	15	-0.07576619057241984
1112	16	0.20582296281131818
1112	17	0.883842291335835
1112	18	0.37588999945220536
1112	19	0.29363001546583334
1112	20	0.09029963573306077
1112	21	0.2186807506928865
1112	22	0.0066905687060270225
1112	23	0.7830624410159395
1112	24	0.6115575010428564
1112	25	0.6171511551199735
1113	1	0.19743076563724316
1113	2	0.41882772644414956
1113	3	0.275867086063986
1113	4	0.17160841166239393
1113	5	0.20638668827365336
1113	6	-0.3363625806227688
1113	7	-0.2289167683335087
1113	8	-0.2199287111541004
1113	9	-0.416298687470654
1113	10	0.07017281609149606
1113	11	-0.29836610788687423
1113	12	-0.3294735304719012
1113	13	-0.4590028391587353
1113	14	0.21503958747218732
1113	15	-0.07063136596497799
1113	16	-0.3375251697863071
1113	17	-0.15755545898686307
1113	18	-0.16820282455948288
1113	19	-0.49814717286307797
1113	20	0.19913096667392338
1113	21	0.3617820712789914
1113	22	-0.2809092554049535
1113	23	-4.081183860350038
1113	24	-1.562352767187896
1113	25	-1.0324041577213414
1114	1	-1.226284727043769
1114	2	-1.4071866168115719
1114	3	-1.2061056028052555
1114	4	-1.502622848775765
1114	5	-1.0614195795326147
1114	6	-2.0697352105471225
1114	7	-2.73643224136622
1114	8	-2.3708276206736976
1114	9	-2.4047061931195373
1114	10	-1.806141163670759
1114	11	-1.991066766104776
1114	12	-3.3453886498352965
1114	13	-2.559209146261594
1114	14	-4.129002066914293
1114	15	-3.3564158241632285
1114	16	-3.493729119093704
1114	17	-3.1817228624646106
1114	18	-3.3032848561932404
1114	19	-3.5681933463575284
1114	20	-3.17624971905159
1114	21	-2.8381393355389655
1114	22	-3.2432827695026027
1114	23	-7.23984384659623
1114	24	-4.889889853345672
1114	25	-4.340784155756513
1115	1	-2.6607317614908013
1115	2	-2.4563522659772197
1115	3	-2.2648542615539142
1115	4	-2.884611230764147
1115	5	-2.3858211039341377
1115	6	-2.3989689397808505
1115	7	-3.329106234040214
1115	8	-4.0383378881839676
1115	9	-4.369800358213702
1115	10	-5.073414630944228
1115	11	-3.987766362804372
1115	12	-4.20053350498015
1115	13	-3.656467443519894
1115	14	-4.54499348290571
1115	15	-4.032221499968905
1115	16	-4.020380245744832
1115	17	-4.135791016532764
1115	18	-6.468571421479805
1115	19	-4.977798555962739
1115	20	-4.804658347460219
1115	21	-4.334184031583661
1115	22	-4.070085596305432
1115	23	-4.595318002070385
1115	24	-4.450092613548431
1115	25	-4.1964470114193695
1116	1	-0.7786071445347247
1116	2	-0.4379734537781843
1116	3	-1.1747185545642758
1116	4	-0.4665461497777166
1116	5	-0.8806919634117385
1116	6	-3.3338441769032805
1116	7	-1.9128943560305842
1116	8	-2.128342265828792
1116	9	-2.890645243665329
1116	10	-2.5576697017161507
1116	11	-2.0305286566902616
1116	12	-2.232380871097181
1116	13	-3.1487846768483614
1116	14	-5.8854283289585325
1116	15	-4.0803026323197
1116	16	-2.538408846357701
1116	17	-3.305366384984538
1116	18	-2.546194360338699
1116	19	-2.287830658691469
1116	20	-3.0422185400765898
1116	21	-3.707429325615472
1116	22	-3.1546835825888095
1116	23	-2.073889430641813
1116	24	-3.03904635643476
1116	25	-2.5617979526130057
1201	1	1.3610028257610267
1201	2	1.2318250739242558
1201	3	1.3384223468951078
1201	4	1.6729346716093403
1201	5	1.1977659986184825
1201	6	1.882028223974931
1201	7	2.1592353094737433
1201	8	2.02056642589276
1201	9	2.4924104195143144
1201	10	2.5091648585318147
1201	11	2.404993750645396
1201	12	1.7105353112610793
1201	13	2.2951969840066013
1201	14	2.671494052547345
1201	15	2.5264066534868377
1201	16	2.2555340698246553
1201	17	2.845318104231527
1201	18	2.286041207270756
1201	19	2.3855840384543363
1201	20	2.3041265458074314
1201	21	2.647149003197651
1201	22	2.5029103732422655
1201	23	2.5985243624616317
1201	24	2.6131556324584366
1201	25	2.389125462084138
1202	1	1.0614645607055202
1202	2	0.9147507051257513
1202	3	1.1443217476220937
1202	4	0.9532176070646905
1202	5	1.1608026426896068
1202	6	0.938811797999886
1202	7	1.2008896959557163
1202	8	1.1078048579587794
1202	9	1.3873165989032543
1202	10	1.487306129776531
1202	11	1.2368534618154534
1202	12	1.3412210367743924
1202	13	0.8510472639948148
1202	14	1.0675791296669033
1202	15	0.4868890191416142
1202	16	0.9336213082567255
1202	17	1.408020727278977
1202	18	1.4744929215845417
1202	19	0.9731171949530157
1202	20	0.5416989988971288
1202	21	0.9681284707288391
1202	22	1.0644095381897003
1202	23	1.3669937602413782
1202	24	1.3009933375375198
1202	25	1.5367087217363675
1203	1	1.024452442060749
1203	2	1.351810999328901
1203	3	0.9493519404073893
1203	4	1.33775823650328
1203	5	1.1509931022678224
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2211	19	-0.6596277749024395
2211	20	-0.6751561216407111
2211	21	-0.5281871955584982
2211	22	-0.5004597547249148
2211	23	-0.36213305670413476
2211	24	-0.5217308902858591
2211	25	-0.2973630925054209
2212	1	-0.5753977584772407
2212	2	-0.56661272375149
2212	3	-0.8078118564452126
2212	4	-1.3129674049766749
2212	5	-1.047756958135185
2212	6	-1.2450118471497262
2212	7	-1.2756729270157479
2212	8	-1.1804559910755508
2212	9	-1.090859409659494
2212	10	-1.0961885421159288
2212	11	-1.675339011150502
2212	12	-1.5240966821344877
2212	13	-1.1436167582935148
2212	14	-1.436828167558076
2212	15	-1.3651123660089404
2212	16	-1.7489588494615589
2212	17	-1.3608322736182217
2212	18	-0.7415354740018678
2212	19	-0.8472539624347206
2212	20	-0.8133402732692154
2212	21	-0.5597634245663698
2212	22	-0.7565676225664646
2212	23	-0.7796905356141899
2212	24	-0.567291436824604
2212	25	-0.6272427350327732
2213	1	-0.0707419759954
2213	2	0.05359957992968134
2213	3	-0.11572979051933174
2213	4	-0.40755738573277256
2213	5	0.037847362430955656
2213	6	0.146492901748406
2213	7	-0.5786246931092336
2213	8	-0.19856698492991498
2213	9	-1.1771620975979147
2213	10	-0.672838241041994
2213	11	-1.0361466342183512
2213	12	-1.0891224441308864
2213	13	-0.7274363622416189
2213	14	-0.6663272053630287
2213	15	-0.8443475761399242
2213	16	-0.4400942396161298
2213	17	-0.13401807037779534
2213	18	-0.5799022255072366
2213	19	-0.9142351530734913
2213	20	-0.8905331468181643
2213	21	-1.1933843817952479
2213	22	-0.9777172904539775
2213	23	-0.7368107789564213
2213	24	-0.40983287328869267
2213	25	-0.47191031216356905
2214	1	-4.223069423828463
2214	2	-2.3592094088343636
2214	3	-2.6913477680474216
2214	4	-4.23681902297194
2214	5	-2.7050418831549194
2214	6	-2.497150757962668
2214	7	-2.6091062340402154
2214	8	-2.3487274985735773
2214	9	-2.354995163408507
2214	10	-2.449258786788384
2214	11	-2.146467661505671
2214	12	-2.1706633751100206
2214	13	-2.8484154954679433
2214	14	-2.3036947816070104
2214	15	-3.0657279934753987
2214	16	-2.5349257002902874
2214	17	-4.393713094454842
2214	18	-3.9984415513499343
2214	19	-3.072084270248453
2214	20	-2.924658347460216
2214	21	-3.348729486129116
2214	22	-3.975020661240496
2214	23	-3.5096037163561
2214	24	-4.78697573043155
2214	25	-4.339823634795991
2215	1	-1.7778246964110345
2215	2	-4.101716372622665
2215	3	-2.0732976287716554
2215	4	-4.27490895532371
2215	5	-2.3228612225898573
2215	6	-2.660923141567924
2215	7	-2.317247897390793
2215	8	-1.5209121384462385
2215	9	-4.522229544626178
2215	10	-2.4002098413048962
2215	11	-1.7706698103317748
2215	12	-1.4992348036814498
2215	13	-1.2673490410310144
2215	14	-1.5213708659683292
2215	15	-1.540053500837118
2215	16	-1.4398282072540738
2215	17	-1.7428018330143331
2215	18	-1.4914777630490974
2215	19	-1.8136123243670355
2215	20	-1.5925612523547663
2215	21	-2.1622272975099364
2215	22	-2.316079157062223
2215	23	-2.5146972300847104
2215	24	-2.380584962425182
2215	25	-3.2814233164513844
2216	1	-3.622643562426994
2216	2	-3.4078814955552303
2216	3	-4.604410098670726
2216	4	-5.216888709383088
2216	5	-4.47654014099464
2216	6	-3.3252421245906234
2216	7	-4.693488735008081
2216	8	-2.9270085670985466
2216	9	-3.7141356976709936
2216	10	-2.801693939711342
2216	11	-3.392081289070518
2216	12	-2.9409692210256217
2216	13	-3.3695072492426235
2216	14	-4.336145420399109
2216	15	-3.969785291191233
2216	16	-3.543760388637171
2216	17	-3.5434072485392427
2216	18	-2.9903114700491216
2216	19	-3.5857737785721078
2216	20	-4.132958773321619
2216	21	-3.6546218593385618
2216	22	-3.1729610406442887
2216	23	-3.241233604083547
2216	24	-3.869182466784629
2216	25	-3.6850965732396617
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19767.0
3	0.0
4	138.0
5	905.0
6	2055.0
7	2149.0
8	1775.0
9	1473.0
10	1183.0
11	1047.0
12	969.0
13	813.0
14	683.0
15	670.0
16	680.0
17	711.0
18	774.0
19	948.0
20	1348.0
21	1812.0
22	2257.0
23	2755.0
24	3133.0
25	3274.0
26	3291.0
27	3485.0
28	3684.0
29	4048.0
30	4749.0
31	5621.0
32	6896.0
33	8401.0
34	10459.0
35	13746.0
36	18530.0
37	28972.0
38	63583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.87799656776887	17.452187605045733	11.674893406224015	18.99492242096138
2	18.330474203890475	21.311171772674957	37.39873610308742	22.959617920347153
3	22.807512689459923	30.244352032739023	25.587066673134508	21.361068604666546
4	13.635241094029759	17.499272352022864	34.31190961289128	34.5535769410561
5	14.059288921033367	40.04383605140372	29.09011527911305	16.806759748449863
6	29.492621880184984	33.038267377413774	19.860910085783882	17.608200656617363
7	27.835902233244052	34.49194911327723	18.877773904448397	18.794374749030318
8	29.20195791973235	29.474283545264758	21.500483300745472	19.823275234257416
9	31.529082621032334	13.19209401049366	18.541742971497662	36.73708039697634
10	22.086639540323223	27.062940766304493	24.930492422570765	25.919927270801523
11	34.82416694982944	25.835035987414333	18.811863711182795	20.52893335157343
12	25.846157920055784	27.65918007970589	25.798493267367807	20.696168732870515
13	33.21816103822749	21.929952298371262	23.878614578782702	20.97327208461854
14	24.810022682541504	26.501504814523884	25.419891794568546	23.268580708366063
15	29.971184099624466	25.910480956352515	24.411877622886795	19.70645732113622
16	22.585015401180904	28.80153217478796	23.78004112865502	24.833411295376116
17	22.656315513048543	27.388040268871066	24.18692100257308	25.76872321550731
18	22.79760958988754	23.221757322175733	29.138198894832545	24.84243419310418
19	22.810021669189588	27.18578571775329	29.838165135995126	20.16602747706199
20	25.91664165798718	21.538210320052254	30.156054159973166	22.389093861987398
21	26.58729458281115	24.106402132278962	29.19660035655658	20.10970292835331
22	25.766743903784615	24.668652763985435	26.977380558314024	22.587222773915922
23	25.192200753797678	24.508133777020646	28.966926465006665	21.332739004175018
24	24.487850516783322	24.691739410575746	27.79111538699171	23.029294685649223
25	23.850733227126096	25.38205921421353	28.935882895485896	21.831324663174478
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	175.0
1	175.0
2	328.5
3	482.0
4	482.0
5	482.0
6	1022.5
7	1563.0
8	1563.0
9	1563.0
10	1506.0
11	1449.0
12	1449.0
13	1449.0
14	1024.5
15	600.0
16	600.0
17	600.0
18	1077.0
19	1554.0
20	1554.0
21	1554.0
22	2566.5
23	3579.0
24	3579.0
25	3579.0
26	6178.5
27	8778.0
28	8778.0
29	8778.0
30	10047.0
31	11316.0
32	11316.0
33	11316.0
34	12486.5
35	13657.0
36	13657.0
37	13657.0
38	12567.5
39	11478.0
40	11478.0
41	11478.0
42	15400.5
43	19323.0
44	19323.0
45	19323.0
46	30270.0
47	41217.0
48	41217.0
49	41217.0
50	39533.5
51	37850.0
52	37850.0
53	37850.0
54	32406.5
55	26963.0
56	26963.0
57	26963.0
58	22459.0
59	17955.0
60	17955.0
61	17955.0
62	16885.0
63	15815.0
64	15815.0
65	15815.0
66	11301.0
67	6787.0
68	6787.0
69	6787.0
70	5354.5
71	3922.0
72	3922.0
73	3922.0
74	2773.0
75	1624.0
76	1624.0
77	1624.0
78	1064.0
79	504.0
80	504.0
81	504.0
82	312.5
83	121.0
84	121.0
85	121.0
86	81.0
87	41.0
88	41.0
89	41.0
90	29.5
91	18.0
92	18.0
93	18.0
94	12.0
95	6.0
96	6.0
97	6.0
98	6.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30513616480880484
2	0.011023705376040638
3	0.009259912515874135
4	0.011464653591082263
5	0.013228446451248765
6	0.07407930012699308
7	0.07187455905178496
8	0.09568576266403274
9	0.07540214477211796
10	0.08378016085790885
11	0.07804783406236772
12	0.08863059122336672
13	0.07363835191195146
14	0.07848878227740934
15	0.07628404120220121
16	0.07848878227740934
17	0.09127628051361648
18	0.09348102158882461
19	0.08598490193311698
20	0.0864258501481586
21	0.07584309298715959
22	0.09215817694369972
23	0.08730774657824185
24	0.08378016085790885
25	0.08069352335261747
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	226784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.43438690560181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.04301484583175	26.28580499506138
2	9.216107455544076	5.978375899534359
3	3.368861819566045	3.2780090306194443
4	1.6191745064984502	2.100677296458304
5	0.9815650660721082	1.5918230563002682
6	0.6036217303822937	1.1746860448708902
7	0.41465006253738645	0.9414244391138705
8	0.3561912012616238	0.924227458727247
9	0.21752134428190767	0.6349654296599407
>10	1.5484800696068302	9.91472061521095
>50	0.2583065963347654	5.911351770848031
>100	0.31540594920876613	20.347114434880766
>500	0.03670672684757192	8.606427261182446
>1k	0.01903311762466692	9.96675250458586
>5k	0.0013595084017619227	2.3436397629462395
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5315	2.3436397629462395	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3298	1.454247213207281	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3089	1.3620890362635811	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2652	1.169394666290391	No Hit
GGATACCACGTGTCCCGCCCTACTC	1692	0.7460843798504303	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1545	0.6812649922393114	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1412	0.6226188796387753	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1185	0.5225236348243263	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1152	0.5079723437279525	No Hit
CCCATGTACTCTGCGTTGATACCAC	1152	0.5079723437279525	No Hit
ATCATTAACTGAATCCATAGGTTAA	1137	0.5013581205023282	No Hit
ATACAGGGTGACAGCCCCGTACACA	1103	0.4863658811909129	No Hit
GTACATGGGGGTTAAGCGACTAAGC	1102	0.4859249329758713	No Hit
GTTAATGATAGTGTGTCGAAACACA	1077	0.4749012275998307	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	1007	0.44403485254691694	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	999	0.44050726682658387	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	990	0.4365387328912093	No Hit
CCCATATTCAGACAGGATACCACGT	941	0.4149322703541696	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	915	0.40346761676308734	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	913	0.4025857203330041	No Hit
TATTCAGACAGGATACCACGTGTCC	887	0.3911210667419218	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	843	0.3717193452800903	No Hit
GTGGATGCCCTGGCAGTCAGAGGCG	827	0.3646641738394243	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	823	0.3629003809792578	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	754	0.33247495414138567	No Hit
GTACTTAGATGTTTCAGTTCCCCCG	745	0.328506420206011	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	727	0.3205693523352618	No Hit
AGCGTACACGGTGGATGCCCTGGCA	674	0.2971990969380556	No Hit
GGTATCAACGCAGAGTACATGGGGG	671	0.29587625229293074	No Hit
CGGTACTGGTTCACTATCGGTCAGT	664	0.2927896147876394	No Hit
TCACGGTACTGGTTCACTATCGGTC	664	0.2927896147876394	No Hit
GAGTACATGGGAAGCAGTGGTATCA	649	0.28617539156201494	No Hit
GTATCAACGCAGAGTACATGGGGGT	643	0.2835297022717652	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	628	0.27691547904614083	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	615	0.2711831522505997	No Hit
GGTTCACTATCGGTCAGTCAGGAGT	607	0.2676555665302667	No Hit
TCATTAACCTATGGATTCAGTTAAT	591	0.2606003950896007	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	572	0.2522223790038098	No Hit
GAGTACATGGGGGTTAAGCGACTAA	571	0.2517814307887682	No Hit
CATCTAAGTACCCCGAGGAAAAGAA	568	0.25045858614364325	No Hit
GGGTACTTAGATGTTTCAGTTCCCC	528	0.23282065754197828	No Hit
GTTATAACCGGCGATTTCCGAATGG	509	0.2244426414561874	No Hit
GTGCTAATCTGCGATAAGCGTCGGT	496	0.21871031466064622	No Hit
ACGCAGAGTACATGGGGGTTAAGCG	489	0.21562367715535485	No Hit
GGAGTATTTAGCCTTGGAGGATGGT	484	0.21341893608014675	No Hit
GTGGTATCAACGCAGAGTACATGGG	470	0.20724566106956396	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	470	0.20724566106956396	No Hit
CCTATGGATTCAGTTAATGATAGTG	467	0.20592281642443913	No Hit
GTCCCGCCCTACTCATCGAGCTCAC	458	0.2019542824890645	No Hit
GTGGTATCAACGCAGAGTACTTTTT	454	0.200190489628898	No Hit
GATATGAACCGTTATAACCGGCGAT	445	0.19622195569352335	No Hit
GCCCTACTCATCGAGCTCACAGCAT	444	0.1957810074784817	No Hit
CCCCAGTAGCGGCGAGCGAACGGGG	415	0.1829935092422746	No Hit
GCCTGAATCAGTGTGTGTGTTAGTG	403	0.1777021306617751	No Hit
GGGTTTCCCCATTCGGAAATCGCCG	402	0.17726118244673345	No Hit
CACACACACTGATTCAGGCTCTGGG	399	0.17593833780160859	No Hit
AGCTCACAGCATGTGCATTTTTGTG	399	0.17593833780160859	No Hit
CATGTACTCTGCGTTGATACCACTG	398	0.17549738958656697	No Hit
GGTGATATGAACCGTTATAACCGGC	398	0.17549738958656697	No Hit
GAATCAGTGTGTGTGTTAGTGGAAG	377	0.16623747707069284	No Hit
CACTATCATTAACTGAATCCATAGG	376	0.1657965288556512	No Hit
GTATCAACGCAGAGTACATGGGAAG	371	0.16359178778044306	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG	353	0.15565471990969382	No Hit
GTGGTATCCTGTCTGAATATGGGGG	351	0.15477282347961055	No Hit
TTCCAGACGCTTCCACTAACACACA	348	0.1534499788344857	No Hit
GTCCCCCCATATTCAGACAGGATAC	345	0.1521271341893608	No Hit
GGTATCAACGCAGAGTACATGGGGA	344	0.15168618597431918	No Hit
GGTATCAACGCAGAGTACATGGGAA	344	0.15168618597431918	No Hit
TTTGTGTACGGGGCTGTCACCCTGT	341	0.15036334132919432	No Hit
GCGTTGATACCACTGCTTCCCATGT	337	0.1485995484690278	No Hit
CCGTTATAACCGGCGATTTCCGAAT	336	0.14815860025398617	No Hit
ATCCATAGGTTAATGAGGCGAACCG	332	0.1463948073938197	No Hit
TATCAACGCAGAGTACATGGGGGTT	328	0.14463101453365318	No Hit
AGTCAGGAGTATTTAGCCTTGGAGG	324	0.14286722167348664	No Hit
GGGGTTAAGCGACTAAGCGTACACG	320	0.14110342881332016	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	316	0.13933963595315366	No Hit
TATCAACGCAGAGTACATGGGAAGC	316	0.13933963595315366	No Hit
CTATCATTAACTGAATCCATAGGTT	316	0.13933963595315366	No Hit
GGGTGACAGCCCCGTACACAAAAAT	312	0.13757584309298715	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA	310	0.1366939466629039	No Hit
TAAGTACCCCGAGGAAAAGAAATCA	305	0.13448920558769578	No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA	304	0.13404825737265416	No Hit
GCGTACACGGTGGATGCCCTGGCAG	304	0.13404825737265416	No Hit
GTATCGCGCGCCTTTCCAGACGCTT	301	0.1327254127275293	No Hit
ATGATAGTGTGTCGAAACACACTGG	291	0.12831593057711302	No Hit
GTACATGGGCATCTAAGTACCCCGA	283	0.12478834485678003	No Hit
CATTAACCTATGGATTCAGTTAATG	277	0.12214265556653027	No Hit
ATTTAGCCTTGGAGGATGGTCCCCC	275	0.121260759136447	No Hit
TCGGTAAGGTGATATGAACCGTTAT	271	0.11949696627628051	No Hit
TAATGATAGTGTGTCGAAACACACT	270	0.1190560180612389	No Hit
GGAGGATGGTCCCCCCATATTCAGA	266	0.11729222520107238	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC	266	0.11729222520107238	No Hit
CCCTCACGGTACTGGTTCACTATCG	259	0.11420558769578101	No Hit
CAGTAGCGGCGAGCGAACGGGGAGC	257	0.11332369126569775	No Hit
GATTCAGGCTCTGGGCTGCTCCCCG	256	0.11288274305065614	No Hit
CCCTGGCAGTCAGAGGCGATGAAGG	252	0.11111895019048965	No Hit
GGGCTGTCACCCTGTATCGCGCGCC	243	0.10715041625511501	No Hit
CCACGTGTCCCGCCCTACTCATCGA	239	0.10538662339494849	No Hit
AAGTACCCCGAGGAAAAGAAATCAA	239	0.10538662339494849	No Hit
ATTCAGACAGGATACCACGTGTCCC	236	0.10406377874982362	No Hit
GTGCATTTTTGTGTACGGGGCTGTC	235	0.10362283053478198	No Hit
GAATATGGGGGGACCATCCTCCAAG	234	0.10318188231974038	No Hit
CTCCAAGGCTAAATACTCCTGACTG	234	0.10318188231974038	No Hit
GGGAAACCCAGTGTGTTTCGACACA	233	0.10274093410469876	No Hit
GTACACAAAAATGCACATGCTGTGA	233	0.10274093410469876	No Hit
ACTCTGCGTTGATACCACTGCTTCC	232	0.10229998588965712	No Hit
TCCCCCAGTAGCGGCGAGCGAACGG	232	0.10229998588965712	No Hit
AGGTTAATGAGGCGAACCGGGGGAA	232	0.10229998588965712	No Hit
GGTATCAACGCAGAGTACATGGGCA	231	0.10185903767461549	No Hit
TTTCAGTTCCCCCGGTTCGCCTCAT	231	0.10185903767461549	No Hit
GTGTTTCGACACACTATCATTAACT	231	0.10185903767461549	No Hit
CTGTCACCCTGTATCGCGCGCCTTT	229	0.10097714124453225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAACTC	25	0.0060274433	18.99404	5
ACCCGCC	25	0.0060274433	18.99404	8
AGACCCG	35	0.0021670489	16.280605	5
TATAGAC	35	0.0021670489	16.280605	2
CCGCCGT	35	0.0021670489	16.280605	10
GTACCGA	40	0.005271665	14.245529	6
GACCCGA	40	0.005271665	14.245529	6
TACCGAG	40	0.005271665	14.245529	7
GAAATCG	65	7.9685205E-4	11.693802	16
GGTATCA	1195	0.0	11.551631	1
TGTCCCG	355	0.0	11.505973	11
CGCCCTA	400	0.0	11.163927	16
CGGTGAT	60	0.0058438606	11.08475	17
CCCGCCC	400	0.0	10.926396	14
CCGCCCT	410	0.0	10.891637	15
GTGTCCC	375	0.0	10.889915	10
AAATCGC	70	0.0014813147	10.858531	17
AATCGCC	70	0.0014813147	10.858531	18
ATCGCCG	70	0.0014840484	10.856133	19
TCCCGCC	405	0.0	10.791503	13
>>END_MODULE