FastQCFastQC Report
Thu 26 May 2016
SRR1512851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226784
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70843.123677155354875No Hit
TATCAACGCAGAGTACTTTTTTTTT51012.2492768449273317No Hit
GGTATCAACGCAGAGTACTTTTTTT45081.9877945534076478No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25091.1063390715394383No Hit
GTACTGGTTCACTATCGGTCAGTCA23591.0401968392831944No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15890.700666713701143No Hit
GGATACCACGTGTCCCGCCCTACTC15670.6909658529702272No Hit
GTACATGGGAAGCAGTGGTATCAAC13230.5833744885000706No Hit
CCCATGTACTCTGCGTTGATACCAC13210.5824925920699873No Hit
ACTTAGATGTTTCAGTTCCCCCGGT12390.546334838436574No Hit
ATCATTAACTGAATCCATAGGTTAA12310.542807252716241No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12220.5388387187808664No Hit
ATACAGGGTGACAGCCCCGTACACA11570.5101770848031607No Hit
CCCATATTCAGACAGGATACCACGT11290.49783053478199524No Hit
GTTAATGATAGTGTGTCGAAACACA10700.4718145900945393No Hit
ACCCTGTATCGCGCGCCTTTCCAGA10560.46564131508395656No Hit
TATTCAGACAGGATACCACGTGTCC10420.45946804007337383No Hit
GTATTTAGCCTTGGAGGATGGTCCC10170.44844433469733314No Hit
GTTCACTATCGGTCAGTCAGGAGTA9640.425074079300127No Hit
TCTAAGTACCCCGAGGAAAAGAAAT9080.400380979257796No Hit
CTATCGGTCAGTCAGGAGTATTTAG9080.400380979257796No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8850.3902391703118386No Hit
CTTATACACATCTCCGAGCCCACGA8430.3717193452800903No Hit
GTACATGGGGGTTAAGCGACTAAGC8010.353199520248342No Hit
TCACGGTACTGGTTCACTATCGGTC7950.3505538309580923No Hit
GCCTTGGAGGATGGTCCCCCCATAT7910.34879003809792575No Hit
ATCAACGCAGAGTACTTTTTTTTTT7630.3364434880767603No Hit
GGTTAATGAGGCGAACCGGGGGAAC7330.3232150416255115No Hit
TCATTAACCTATGGATTCAGTTAAT7090.3126322844645125No Hit
CGGTACTGGTTCACTATCGGTCAGT7010.30910469874417945No Hit
GTCAGGAGTATTTAGCCTTGGAGGA6720.29631720050797233No Hit
AGCGTACACGGTGGATGCCCTGGCA6540.2883801326372231No Hit
GTACTTAGATGTTTCAGTTCCCCCG6420.28308875405672357No Hit
GCCCAGAGCCTGAATCAGTGTGTGT6180.2725059968957246No Hit
CATCTAAGTACCCCGAGGAAAAGAA6100.26897841117539156No Hit
GAGTACATGGGAAGCAGTGGTATCA5740.2531042754338931No Hit
GTATCAACGCAGAGTACATGGGGGT5730.25266332721885143No Hit
GTGGTATCAACGCAGAGTACTTTTT5620.24781289685339353No Hit
GTGGATGCCCTGGCAGTCAGAGGCG5600.2469310004233103No Hit
GGTATCAACGCAGAGTACATGGGGG5600.2469310004233103No Hit
GGTTCACTATCGGTCAGTCAGGAGT5540.24428531113306054No Hit
ACGCAGAGTACATGGGGGTTAAGCG5110.22532453788627066No Hit
CCTATGGATTCAGTTAATGATAGTG5070.22356074502610412No Hit
CCCCAGTAGCGGCGAGCGAACGGGG5000.22047410752081276No Hit
ATACACATCTCCGAGCCCACGAGAC4940.217828418230563No Hit
GAGTACATGGGGGTTAAGCGACTAA4750.20945040214477212No Hit
GTTATAACCGGCGATTTCCGAATGG4670.20592281642443913No Hit
CACACACACTGATTCAGGCTCTGGG4550.2006314378439396No Hit
GGAGTATTTAGCCTTGGAGGATGGT4530.19974954141385634No Hit
CATGTACTCTGCGTTGATACCACTG4340.19137152532806548No Hit
GATATGAACCGTTATAACCGGCGAT4330.19093057711302386No Hit
TATCAACGCAGAGTACATGGGAAGC4280.18872583603781573No Hit
GCCCTACTCATCGAGCTCACAGCAT4160.1834344574573162No Hit
GTGGTATCAACGCAGAGTACATGGG4120.18167066459714973No Hit
GCCTGAATCAGTGTGTGTGTTAGTG4090.1803478199520248No Hit
GTCCCGCCCTACTCATCGAGCTCAC4090.1803478199520248No Hit
CACTATCATTAACTGAATCCATAGG4040.17814307887681669No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG3920.1728517002963172No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3870.17064695922110906No Hit
GGGTACTTAGATGTTTCAGTTCCCC3820.16844221814590093No Hit
TTCCAGACGCTTCCACTAACACACA3790.16711937350077608No Hit
ATGATAGTGTGTCGAAACACACTGG3770.16623747707069284No Hit
TTTGTGTACGGGGCTGTCACCCTGT3740.16491463242556795No Hit
ACACATCTCCGAGCCCACGAGACCA3710.16359178778044306No Hit
AGCTCACAGCATGTGCATTTTTGTG3650.1609460984901933No Hit
GTGCTAATCTGCGATAAGCGTCGGT3640.16050515027515166No Hit
AGTCAGGAGTATTTAGCCTTGGAGG3580.15785946098490194No Hit
TATCAACGCAGAGTACATGGGGGTT3570.1574185127698603No Hit
GTATCAACGCAGAGTACATGGGAAG3540.15609566812473544No Hit
GGGTTTCCCCATTCGGAAATCGCCG3420.15080428954423591No Hit
ACGCAGAGTACATGGGAAGCAGTGG3390.14948144489911105No Hit
GCGTTGATACCACTGCTTCCCATGT3380.1490404966840694No Hit
TCCCCCAGTAGCGGCGAGCGAACGG3290.1450719627486948No Hit
TAATGATAGTGTGTCGAAACACACT3270.14419006631861156No Hit
CTATCATTAACTGAATCCATAGGTT3250.1433081698885283No Hit
TAAGTACCCCGAGGAAAAGAAATCA3220.1419853252434034No Hit
GTCCCCCCATATTCAGACAGGATAC3120.13757584309298715No Hit
CAACGCAGAGTACTTTTTTTTTTTT3110.13713489487794553No Hit
GGTATCAACGCAGAGTACATGGGGA3080.13581205023282067No Hit
GGTATCAACGCAGAGTACATGGGAA3070.13537110201777902No Hit
ATCCATAGGTTAATGAGGCGAACCG3030.13360730915761254No Hit
CATTAACCTATGGATTCAGTTAATG2980.1314025680824044No Hit
ATTTAGCCTTGGAGGATGGTCCCCC2940.1296387752222379No Hit
GCGTACACGGTGGATGCCCTGGCAG2930.12919782700719626No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2920.12875687879215467No Hit
CCACGTGTCCCGCCCTACTCATCGA2890.12743403414702978No Hit
CCGTTATAACCGGCGATTTCCGAAT2870.12655213771694654No Hit
ACTCTGCGTTGATACCACTGCTTCC2810.12390644842669676No Hit
CCCTCACGGTACTGGTTCACTATCG2760.12170170735148864No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC2760.12170170735148864No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2740.1208198109214054No Hit
CCCTGGCAGTCAGAGGCGATGAAGG2730.12037886270636376No Hit
TCGGTAAGGTGATATGAACCGTTAT2630.11596938055594751No Hit
GTACATGGGCATCTAAGTACCCCGA2580.11376463948073938No Hit
ATTCAGACAGGATACCACGTGTCCC2520.11111895019048965No Hit
TTTCAGTTCCCCCGGTTCGCCTCAT2510.11067800197544801No Hit
GGTGATATGAACCGTTATAACCGGC2490.10979610554536474No Hit
CTGTCACCCTGTATCGCGCGCCTTT2490.10979610554536474No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2490.10979610554536474No Hit
GGGGTTAAGCGACTAAGCGTACACG2400.10582757160999012No Hit
AAGTACCCCGAGGAAAAGAAATCAA2380.10494567517990688No Hit
GTATCGCGCGCCTTTCCAGACGCTT2370.10450472696486525No Hit
GGAGGATGGTCCCCCCATATTCAGA2350.10362283053478198No Hit
CAGTTAATGATAGTGTGTCGAAACA2350.10362283053478198No Hit
CTAACACACACACTGATTCAGGCTC2340.10318188231974038No Hit
GTACGGGGCTGTCACCCTGTATCGC2320.10229998588965712No Hit
TCAACGCAGAGTACTTTTTTTTTTT2300.10141808945957385No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2280.10053619302949061No Hit
CTACTGGGGGAATCTCGGTTGATTT2270.100095244814449No Hit
GTGCATTTTTGTGTACGGGGCTGTC2270.100095244814449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACAAC250.00602583118.9949269
TGCTTGG250.006033517418.99073614
GCTTGGT250.006033517418.99073615
GGCATCT453.5188124E-516.8881038
ACCAGTA402.7259873E-416.6425882
CTTATAC1200.016.6389141
GGAGACA601.444052E-615.8430857
TTATACA1300.015.362392
CCAGTAC508.583872E-515.2160813
CAGTACC508.616301E-515.2093614
AGTACCG508.616301E-515.2093615
TACCGTG456.6994765E-414.786887
TATACAC1400.014.2650753
AGGGAGA602.5344427E-514.2587775
CAACCCC801.2795499E-714.24305212
ACAGGGA759.4546704E-713.9480743
ACCCCGT759.621035E-713.9265414
CAGGGAG551.93839E-413.8266934
CGAATGG707.2501916E-613.5618219
AACCAGT500.001483277713.3111311
Produced by FastQC (version 0.11.5)