FastQCFastQC Report
Thu 26 May 2016
SRR1512850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087269
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT104080.4986420054147309No Hit
TATCAACGCAGAGTACTTTTTTTTT60100.2879360542412118No Hit
GGTATCAACGCAGAGTACTTTTTTT57800.27691687080103233No Hit
GTACTGGTTCACTATCGGTCAGTCA32060.15359783525745843No Hit
GTCCTACAGTGGACATTTCTAAATT29420.1409497290478611No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28340.13577550378029857No Hit
GTCCTAAAGTGTGTATTTCTCATTT23900.11450368879143034No Hit
CTGTAGGACGTGGAATATGGCAAGA23090.11062301984075844No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22700.10875454960524973No Hit
CTTTAGGACGTGAAATATGGCGAGG21710.10401150977665075No Hit
GTACATGGGAAGCAGTGGTATCAAC21260.10185558258183301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1201.00444595E-811.87305218
TTAGACT1052.7271926E-711.75997454
CCAACGA907.477516E-611.60920619
GGTATCA24250.011.4730641
TAGGACC5900.011.4303274
GTCCTAA8550.011.2554681
GTGTTAC1752.0008883E-1110.8893081
ACCGATC700.001493815410.8553628
CCATGCG700.001494413210.8548419
TCGTCCC700.001494413210.8548419
TGTAGGA16750.010.4908162
TCCTAAA9100.010.3334682
GGCGAGG7450.010.32716419
TAACTAT1201.5273763E-610.2899786
TGGCGAG14950.010.22909118
GGACCTG10200.010.1502956
GTCCTAC16150.010.1476211
GTATCAA50150.010.1456211
AGGACCT11150.010.137355
TCGACAG750.00265182710.1316715
Produced by FastQC (version 0.11.5)