##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512850_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087269
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.37332945585835	31.0	28.0	33.0	16.0	34.0
2	28.401147144905615	31.0	28.0	34.0	16.0	34.0
3	28.601004949529745	31.0	28.0	34.0	16.0	34.0
4	31.97381315010188	35.0	32.0	37.0	19.0	37.0
5	32.187641362948426	35.0	33.0	37.0	22.0	37.0
6	32.01277506636662	35.0	33.0	37.0	19.0	37.0
7	31.946597683384365	35.0	33.0	37.0	18.0	37.0
8	31.866978333889882	35.0	33.0	37.0	17.0	37.0
9	33.079821527555865	38.0	33.0	39.0	17.0	39.0
10	32.96330803552393	38.0	33.0	39.0	16.0	39.0
11	33.001442075745864	38.0	33.0	39.0	16.0	39.0
12	32.89365865156815	38.0	33.0	39.0	12.0	39.0
13	32.84312755088108	38.0	33.0	39.0	11.0	39.0
14	33.64465097694643	39.0	33.0	41.0	10.0	41.0
15	33.81274430847198	39.0	33.0	41.0	10.0	41.0
16	33.74263738885596	39.0	33.0	41.0	10.0	41.0
17	33.50269658582579	39.0	33.0	41.0	10.0	41.0
18	33.500953159367576	39.0	33.0	41.0	8.0	41.0
19	33.62416823131087	39.0	33.0	41.0	8.0	41.0
20	33.607821033129895	39.0	33.0	41.0	2.0	41.0
21	33.50980060548017	39.0	33.0	41.0	2.0	41.0
22	33.50504558827827	39.0	33.0	41.0	2.0	41.0
23	33.16630055828932	39.0	33.0	41.0	2.0	41.0
24	33.23606923688322	39.0	33.0	41.0	2.0	41.0
25	32.947416456623465	39.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8967287958398522
1101	2	0.48011397883484364
1101	3	0.8312057047562718
1101	4	1.0090357314182086
1101	5	0.8522715219588335
1101	6	0.9498603711709137
1101	7	0.6358383301065444
1101	8	0.8334397840605519
1101	9	1.0336520644673541
1101	10	0.9921968540199231
1101	11	0.8874021022742795
1101	12	0.8197740970491267
1101	13	1.273592660516364
1101	14	1.3128780054658478
1101	15	1.0967493798292622
1101	16	1.072878715889857
1101	17	0.5046122664437931
1101	18	0.9501527952529898
1101	19	0.3749274735719581
1101	20	-0.1939937436709016
1101	21	0.9708774055106062
1101	22	0.9771017945879876
1101	23	0.4390653067592467
1101	24	1.0656236544490483
1101	25	0.6667736723113791
1102	1	1.0797461098765027
1102	2	1.1086597442404837
1102	3	1.0592809329210802
1102	4	1.1324696418860967
1102	5	1.0954217615619797
1102	6	0.891050921896948
1102	7	0.9054306435411732
1102	8	0.9480196959251259
1102	9	1.307728207699462
1102	10	1.2896284045773925
1102	11	1.1994280131274593
1102	12	1.235925998221859
1102	13	1.240616524508468
1102	14	1.3632986956192426
1102	15	1.3256144555631266
1102	16	1.3872630923196496
1102	17	1.4661693257064385
1102	18	1.5014794020389317
1102	19	1.374144850218478
1102	20	1.4324921570683813
1102	21	1.5790826695556888
1102	22	1.342144297056258
1102	23	1.2594587857006587
1102	24	0.7696682519398337
1102	25	1.2218096729856356
1103	1	0.6451462351694346
1103	2	0.6622633081282778
1103	3	0.8700060077488025
1103	4	0.9654824728102511
1103	5	0.8063547298443083
1103	6	0.4140458869773411
1103	7	0.2614709228980061
1103	8	0.5170695578708759
1103	9	0.6482762482572468
1103	10	0.8274858954704527
1103	11	0.6709053095844553
1103	12	0.7959000115400343
1103	13	0.9342103433905748
1103	14	0.8384108791965872
1103	15	0.7499523752364325
1103	16	0.8268027568130307
1103	17	0.35115134385070945
1103	18	0.8522699076191103
1103	19	0.6999598343248365
1103	20	1.0007058047168158
1103	21	0.757502586491114
1103	22	0.7526169298319587
1103	23	0.05306049339689167
1103	24	0.2670551982726934
1103	25	0.564122487668115
1104	1	0.5788146241358803
1104	2	0.5185367675769257
1104	3	0.637240616387448
1104	4	0.6797823392323465
1104	5	0.49388678899840954
1104	6	0.4281348409153338
1104	7	0.613313211490194
1104	8	0.47142809631127136
1104	9	0.8276594159801505
1104	10	0.6881216824798813
1104	11	0.4114367542619277
1104	12	0.7171312291442007
1104	13	0.7603297089000876
1104	14	0.9717182517292073
1104	15	0.6389392740103119
1104	16	0.5056493303069018
1104	17	0.756084322071942
1104	18	0.6376652680922845
1104	19	0.17100657198349012
1104	20	0.2891825874523022
1104	21	0.5722954950838357
1104	22	0.45564320441445005
1104	23	0.758172701577557
1104	24	0.4916421156726116
1104	25	0.09220708274809652
1105	1	0.5732392930227945
1105	2	0.5930136960830801
1105	3	0.6973616308491053
1105	4	0.8413369352523787
1105	5	0.6009810748611741
1105	6	0.7197850530989456
1105	7	0.7968716385303409
1105	8	0.7780675956531837
1105	9	0.3640740244105345
1105	10	0.702260496935061
1105	11	0.6825080438642672
1105	12	0.7986650712401868
1105	13	0.7342616431994813
1105	14	0.8612918764648683
1105	15	0.8438400030050204
1105	16	0.9070074430505741
1105	17	1.204172261347793
1105	18	1.0398425289110946
1105	19	0.6151945950753515
1105	20	0.9934763791464221
1105	21	0.7081175429504043
1105	22	0.8059892510200655
1105	23	1.1321699287142408
1105	24	1.1753811572584993
1105	25	1.376983349761403
1106	1	0.6080144857583498
1106	2	0.6512477629709998
1106	3	0.4436548031832963
1106	4	0.6278933795449717
1106	5	0.5016728586826602
1106	6	0.52520783506192
1106	7	0.5309696203842194
1106	8	0.46602424096993644
1106	9	0.5999185112875836
1106	10	0.4639931183297108
1106	11	0.23137047418857293
1106	12	0.3864557948334806
1106	13	0.47961281920575516
1106	14	0.7031207841574698
1106	15	0.3981213941101345
1106	16	0.6107792870069773
1106	17	0.6087283092863984
1106	18	0.5632000668110422
1106	19	0.6576318199830169
1106	20	0.7417503848804472
1106	21	0.6971044838730549
1106	22	0.6562123968195195
1106	23	0.7948818591567388
1106	24	0.5992109483185075
1106	25	0.8159069976716822
1107	1	0.7868601579567134
1107	2	0.7339667967641255
1107	3	0.7035876436422832
1107	4	0.7226371250808157
1107	5	0.6425403670080314
1107	6	0.8699366459484423
1107	7	0.580912015308293
1107	8	0.7909660329423289
1107	9	0.7769864964085897
1107	10	0.982689815082118
1107	11	0.8032493643864314
1107	12	0.8823655561573744
1107	13	0.6303533366310603
1107	14	1.076013746319937
1107	15	0.8525872253022158
1107	16	0.9002265194587693
1107	17	1.128245205847719
1107	18	1.0595402428729344
1107	19	0.8179229065019484
1107	20	1.0140188746256271
1107	21	0.7304282318206958
1107	22	0.7422263296414258
1107	23	1.1196820966269385
1107	24	1.0350459436340387
1107	25	1.2615248376204775
1108	1	0.6467741191165715
1108	2	0.6536349076333181
1108	3	0.6989969195404555
1108	4	0.7194339744120839
1108	5	0.7492319458032242
1108	6	0.8281070501645189
1108	7	0.7636279338079603
1108	8	0.7316802779777873
1108	9	0.8189541748931859
1108	10	0.8304708103114322
1108	11	0.5369715079976984
1108	12	0.7064771087554078
1108	13	0.6496356745744549
1108	14	0.7674806559747722
1108	15	0.8645142061888009
1108	16	0.5475661056487411
1108	17	0.892702753740771
1108	18	0.9243988964789835
1108	19	0.8700049675790069
1108	20	1.0218439406661375
1108	21	0.8700170502761182
1108	22	0.9939211283219294
1108	23	1.196281985354716
1108	24	1.1537442338092205
1108	25	1.322261862796914
1109	1	0.5574476121448484
1109	2	0.48634865969147967
1109	3	0.6862618172581882
1109	4	0.6879339505619484
1109	5	0.4981310966339265
1109	6	0.8052538322588845
1109	7	0.7006092586290578
1109	8	0.6619952286010431
1109	9	0.803518958652532
1109	10	0.7595861164932103
1109	11	0.7352040039997547
1109	12	0.5541645682145244
1109	13	0.7204622228051747
1109	14	0.7807868558611943
1109	15	0.6976579570527193
1109	16	0.3571977133115638
1109	17	0.7650485556467643
1109	18	0.9156702688993832
1109	19	0.720691625186106
1109	20	0.5983486492401013
1109	21	0.6278795871437239
1109	22	0.722890269727472
1109	23	1.0343325100403007
1109	24	0.7455301772701262
1109	25	0.32691620816986244
1110	1	0.6878072512147746
1110	2	0.6805162972545347
1110	3	0.6456645916409158
1110	4	0.7274319733195469
1110	5	0.5867551539120512
1110	6	0.6801380918855742
1110	7	0.4965453561864166
1110	8	0.5458850151943153
1110	9	0.791274023980364
1110	10	0.6966360517844237
1110	11	0.6461542866972678
1110	12	0.6525515574621821
1110	13	0.6850665679765626
1110	14	0.735198376247908
1110	15	0.547347323118224
1110	16	0.7223116335848374
1110	17	0.7669324121001324
1110	18	0.1290711787377674
1110	19	0.5693862854146943
1110	20	0.6770994574385938
1110	21	0.8071951554540959
1110	22	0.7475568580875063
1110	23	0.8483688146869497
1110	24	0.6862203126453608
1110	25	0.8797025485306875
1111	1	0.3468402574591991
1111	2	0.36526364585201776
1111	3	0.40720290069037546
1111	4	0.2811357431018209
1111	5	0.3840528048189782
1111	6	0.15002067027127453
1111	7	0.0318948787687674
1111	8	0.3101412678787554
1111	9	0.42526510337376777
1111	10	0.3977788145293246
1111	11	0.0610779989911876
1111	12	-0.13362559917706562
1111	13	0.236120058576887
1111	14	0.19079318247250399
1111	15	0.24328705946473406
1111	16	0.40392580082968976
1111	17	-0.20673765761275575
1111	18	0.11115992225380467
1111	19	0.2904535864356106
1111	20	0.43490776351451643
1111	21	0.3522214877632095
1111	22	0.13662323400847498
1111	23	0.40898889596358856
1111	24	0.5381681105770966
1111	25	0.6585685081566837
1112	1	0.11691863076884346
1112	2	0.10925759182642025
1112	3	0.09420582899623753
1112	4	0.17587001305432892
1112	5	-0.03380762356913891
1112	6	-0.22495637488166764
1112	7	-0.3358684000563983
1112	8	-0.0814195959166355
1112	9	-0.15157272112529796
1112	10	-0.16268199333213573
1112	11	-0.17703332050123777
1112	12	-0.13821121647929147
1112	13	-0.1240026889855983
1112	14	-0.18310054305639767
1112	15	-0.36615695724963615
1112	16	-0.08239003944054701
1112	17	0.2719193138389997
1112	18	0.04950581330327708
1112	19	0.10927449914577636
1112	20	-0.1141244478499388
1112	21	-0.14664715523572625
1112	22	-0.20132809250311112
1112	23	0.11313287648542314
1112	24	-0.026085149493752624
1112	25	-0.07436376791565635
1113	1	0.11671544771795084
1113	2	0.3107613627469945
1113	3	0.2524388215427358
1113	4	0.22533868577051663
1113	5	0.21576056737605853
1113	6	-0.07269267320919326
1113	7	-0.13944555755796983
1113	8	-0.08938790512986827
1113	9	-0.11380338977691906
1113	10	-0.10833178922511166
1113	11	-0.12534919532641453
1113	12	-0.34828211871029424
1113	13	-0.10237207739469767
1113	14	0.20989820530355985
1113	15	-0.07660335149416397
1113	16	-0.12728069333570602
1113	17	-0.028383522655886395
1113	18	-0.17886544557978112
1113	19	-0.6149101882980261
1113	20	-0.3329217646437783
1113	21	-0.19402711700594466
1113	22	-0.6887121287515399
1113	23	-4.367035457156653
1113	24	-1.8619090076370064
1113	25	-1.0248246932639127
1114	1	-0.7760174311749921
1114	2	-1.032922918936265
1114	3	-0.7924277901724075
1114	4	-0.6821124793326518
1114	5	-0.7590812289282347
1114	6	-1.5087487237645973
1114	7	-1.2939308667475196
1114	8	-1.160339046246257
1114	9	-1.5228991755183081
1114	10	-1.1010720822126885
1114	11	-1.2292991632713601
1114	12	-2.0053802420721105
1114	13	-1.332571865438819
1114	14	-3.0404147131021517
1114	15	-1.9567078466161298
1114	16	-2.051417932588542
1114	17	-1.9930086965600147
1114	18	-1.81749549534144
1114	19	-1.8720780913740036
1114	20	-1.4999639333972965
1114	21	-1.4897108055616357
1114	22	-1.640641755909808
1114	23	-5.957971944533067
1114	24	-3.1518855878204413
1114	25	-2.9733516871102132
1115	1	-1.3901879452674102
1115	2	-1.2398596066543135
1115	3	-1.00483720060355
1115	4	-1.0680731698385948
1115	5	-1.001878717711982
1115	6	-1.1345302484276303
1115	7	-1.638867855242168
1115	8	-2.085772192881411
1115	9	-3.1827915102176583
1115	10	-3.8937763710027546
1115	11	-2.7110838400254735
1115	12	-2.955741069627223
1115	13	-2.275410196623678
1115	14	-2.8435609993709043
1115	15	-2.5250706607281366
1115	16	-2.68604642428334
1115	17	-2.51036393390466
1115	18	-5.231109940490612
1115	19	-3.237678511944381
1115	20	-3.4473798141036873
1115	21	-3.019171257464066
1115	22	-2.60065427203331
1115	23	-3.1364070175896757
1115	24	-2.7897868390650196
1115	25	-3.0202771111122857
1116	1	-1.2925831659642242
1116	2	-0.9130306484422768
1116	3	-1.4719562584687829
1116	4	-1.203292685326609
1116	5	-1.3350823303161548
1116	6	-3.7079092596586563
1116	7	-2.0729779941041038
1116	8	-2.4821790524415626
1116	9	-3.450626788518125
1116	10	-2.683358322607809
1116	11	-2.165380459467272
1116	12	-2.0122962698477345
1116	13	-3.277986047529218
1116	14	-6.563897508218183
1116	15	-4.3186108384331625
1116	16	-3.0502804053765153
1116	17	-4.080387307262406
1116	18	-2.761712950619067
1116	19	-2.688092960110936
1116	20	-3.307963107356354
1116	21	-3.970004738463018
1116	22	-3.6577951844099417
1116	23	-2.724440533465838
1116	24	-3.7767948523858657
1116	25	-3.645687748099572
1201	1	0.6213549249610288
1201	2	0.5692888398527849
1201	3	0.9697431797730935
1201	4	0.8435835049883522
1201	5	0.3807438999228552
1201	6	0.8941706279332919
1201	7	0.9712089802175399
1201	8	1.033409157127231
1201	9	1.4093870367697363
1201	10	1.150841602340627
1201	11	1.3912612934663713
1201	12	1.2920531657137246
1201	13	1.2916293555343543
1201	14	1.4698909310196768
1201	15	1.343948645573235
1201	16	1.405886942679338
1201	17	1.628953565085041
1201	18	1.3074453150691099
1201	19	1.5261572437415225
1201	20	1.6646107944502475
1201	21	1.7699559300037606
1201	22	1.466973467737354
1201	23	1.8739515573409093
1201	24	1.6809731818557054
1201	25	1.7187686218312734
1202	1	1.1158761107679496
1202	2	0.9616720321116645
1202	3	1.0649970685521524
1202	4	1.170559039785104
1202	5	1.0734926736983041
1202	6	1.2572725539927347
1202	7	1.2658112438123652
1202	8	1.3378649128513231
1202	9	1.4527299671311908
1202	10	1.3897600569887487
1202	11	1.2630345197405788
1202	12	1.4904925915397769
1202	13	1.4641896253778626
1202	14	1.8866033306661834
1202	15	1.4813303566033014
1202	16	1.352438355178755
1202	17	1.7268247726724724
1202	18	1.6388054411593131
1202	19	1.659054556182248
1202	20	1.6128264960146907
1202	21	1.8109197508695871
1202	22	1.6858944671346592
1202	23	1.7485400808300824
1202	24	1.7356902941005075
1202	25	2.098732591108721
1203	1	1.115471300716198
1203	2	1.166369667904604
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2211	16	-0.1688547894586776
2211	17	0.27256077348073404
2211	18	-0.18530715781803053
2211	19	0.08350187351500438
2211	20	-0.039954897865982275
2211	21	0.19778768341906527
2211	22	0.21730582693992773
2211	23	0.39589872619638555
2211	24	0.016422591956924748
2211	25	0.34700712426593583
2212	1	-0.135464963424333
2212	2	-0.11180194986418002
2212	3	-0.391364703057949
2212	4	-0.04950727340866834
2212	5	-0.31364197429748586
2212	6	-0.4050453716742872
2212	7	-0.41079900788897206
2212	8	-0.3220037566638574
2212	9	-0.2253413223290437
2212	10	-0.48577718725559293
2212	11	-0.6612705061404469
2212	12	-0.18216330555814153
2212	13	-0.1805367910438207
2212	14	-0.15285875862521436
2212	15	-0.4616701025165213
2212	16	-0.4719970014907062
2212	17	-0.2287787107140531
2212	18	0.04795599543542295
2212	19	-0.3150738566701463
2212	20	-0.12465582416955101
2212	21	-0.04079546895254538
2212	22	0.07689275654306016
2212	23	0.012425308279517822
2212	24	0.042622818296862874
2212	25	0.18314325157480482
2213	1	-0.2204210038020591
2213	2	-0.047150222561850086
2213	3	-0.2634140799266049
2213	4	-0.09702732906125533
2213	5	-0.1944721747060001
2213	6	0.031071084321688147
2213	7	-0.17595915708526633
2213	8	-0.022502557765069042
2213	9	-0.45760058049192054
2213	10	-0.052395996645486775
2213	11	-0.2064083054951169
2213	12	-0.21243549998696665
2213	13	-0.34557409011726037
2213	14	-0.44018743450811115
2213	15	-0.28450764432997033
2213	16	-0.22286435296046392
2213	17	-0.20912195099705144
2213	18	-0.27957945348556734
2213	19	-0.2185802655982414
2213	20	-0.1462145406077866
2213	21	-0.28754364100191765
2213	22	-0.5273611139758714
2213	23	-0.26767149169679527
2213	24	-0.03822810381911523
2213	25	0.17183067941885355
2214	1	-2.8659788869711242
2214	2	-1.4511491735747768
2214	3	-1.7211353740519648
2214	4	-3.7218029363654495
2214	5	-2.1011712527837325
2214	6	-1.7928750895291863
2214	7	-1.6212807059646295
2214	8	-1.4207457385686055
2214	9	-1.2147027068761282
2214	10	-1.2601809012220038
2214	11	-1.2792750529171357
2214	12	-1.2658940353387678
2214	13	-1.594798667010199
2214	14	-1.495661991888639
2214	15	-1.8591613381069152
2214	16	-1.676202769926583
2214	17	-3.8349750334892683
2214	18	-3.6252210839111925
2214	19	-2.4166487713224036
2214	20	-2.261635509110075
2214	21	-2.0249663328401155
2214	22	-2.977673156409896
2214	23	-2.0301769941959478
2214	24	-3.115786804948339
2214	25	-3.1046952689622387
2215	1	-1.73432283666941
2215	2	-3.2328939600297275
2215	3	-1.9393933507334609
2215	4	-3.5269956986847966
2215	5	-2.107732221586737
2215	6	-1.9934389272572908
2215	7	-2.130871749928893
2215	8	-1.543701264308602
2215	9	-3.749775592494519
2215	10	-2.237281901358976
2215	11	-1.689170456360717
2215	12	-1.6213922623920034
2215	13	-1.7453982873552505
2215	14	-1.332397042234959
2215	15	-1.5385673466655305
2215	16	-1.6027501164669147
2215	17	-2.001741765168589
2215	18	-1.5815824753161785
2215	19	-1.6692672378369373
2215	20	-1.6556860026654547
2215	21	-2.12121940140182
2215	22	-2.1254435224031063
2215	23	-2.0044156810271936
2215	24	-1.958354342910873
2215	25	-2.9205681457385886
2216	1	-2.424111457492515
2216	2	-2.4169425923542214
2216	3	-3.8197878863004107
2216	4	-4.248094730128656
2216	5	-3.8482722035060277
2216	6	-2.9174679892173856
2216	7	-4.70030418143029
2216	8	-2.616934027196539
2216	9	-3.28484878274012
2216	10	-2.1314374506869385
2216	11	-2.5020394761262885
2216	12	-2.829820367371834
2216	13	-2.507795877339049
2216	14	-3.4279631722838495
2216	15	-2.7539841138064354
2216	16	-3.1189918740879854
2216	17	-2.683585550460652
2216	18	-2.3819129308190483
2216	19	-3.1269387989567754
2216	20	-3.6782421976354414
2216	21	-3.355947084405365
2216	22	-3.0433091299238875
2216	23	-3.0116988607241666
2216	24	-3.409588725179738
2216	25	-3.2375729799848045
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	127562.0
3	4.0
4	1136.0
5	6743.0
6	8553.0
7	7946.0
8	6919.0
9	6437.0
10	5625.0
11	5295.0
12	4922.0
13	4788.0
14	4550.0
15	4648.0
16	4643.0
17	5040.0
18	5741.0
19	6726.0
20	8832.0
21	11431.0
22	14271.0
23	16979.0
24	19846.0
25	22393.0
26	24613.0
27	26605.0
28	30107.0
29	34843.0
30	41743.0
31	52009.0
32	64295.0
33	81213.0
34	104147.0
35	135789.0
36	183482.0
37	284489.0
38	712904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.279900549028206	18.076252475097597	12.44560073184532	24.19824624402888
2	15.505481663343792	19.996415992149682	40.40623866521582	24.09186367929071
3	19.559277092154506	25.374603930580225	28.6105264267483	26.455592550516972
4	12.559048798660308	16.13314518853211	37.09570229678804	34.212103716019534
5	13.455197974140098	38.44562688816324	34.23564277209316	13.863532365603506
6	32.24958536535078	35.61437131605685	18.048534430139277	14.08750888845309
7	28.796187570093775	31.776578335528544	21.161594342920186	18.265639751457492
8	26.71018895361385	35.0335385710353	20.25858223880153	17.99769023654932
9	28.02627850206209	14.691631339525593	19.56152628593431	37.72056387247801
10	16.759544537980346	28.247037075222202	32.379187236705505	22.61423115009195
11	34.69361920051558	22.76719010436147	23.559491408064662	18.979699287058295
12	24.30353570933589	24.853216293219248	30.048086260814856	20.79516173663001
13	29.46646444526812	21.7636224929909	25.436261659657166	23.333651402083813
14	22.206946818738714	22.094643032681745	26.905004519484134	28.79340562909541
15	24.163295329165134	29.801057122293116	23.289488763460376	22.74615878508137
16	22.895865720552287	27.715851124126207	26.612823564500953	22.775459590820553
17	22.224795963095435	28.093884312106205	26.86951901099291	22.81180071380545
18	22.138427819848165	26.64347067089342	28.976466695122127	22.241634814136287
19	23.77196781035714	26.494017312890865	27.58874523022711	22.14526964652488
20	24.491388223378685	25.996363085315956	27.82685715710666	21.6853915341987
21	24.0821712481186	25.895412570419175	27.111592306478826	22.910823874983397
22	24.273586091066836	26.824871253547784	26.87038452721349	22.031158128171903
23	23.177927920367413	26.735920455651694	27.593456408908747	22.492695215072146
24	23.58373442296082	26.1124572675906	27.831679982506625	22.472128326941952
25	23.30327775697134	26.546748065920546	27.733258398194266	22.416715778913847
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	383.0
1	383.0
2	888.0
3	1393.0
4	1393.0
5	1393.0
6	2605.0
7	3817.0
8	3817.0
9	3817.0
10	4399.0
11	4981.0
12	4981.0
13	4981.0
14	6507.0
15	8033.0
16	8033.0
17	8033.0
18	14633.5
19	21234.0
20	21234.0
21	21234.0
22	33335.0
23	45436.0
24	45436.0
25	45436.0
26	67003.0
27	88570.0
28	88570.0
29	88570.0
30	113133.0
31	137696.0
32	137696.0
33	137696.0
34	156135.0
35	174574.0
36	174574.0
37	174574.0
38	189804.5
39	205035.0
40	205035.0
41	205035.0
42	220901.5
43	236768.0
44	236768.0
45	236768.0
46	257492.0
47	278216.0
48	278216.0
49	278216.0
50	274091.5
51	269967.0
52	269967.0
53	269967.0
54	244376.5
55	218786.0
56	218786.0
57	218786.0
58	191855.0
59	164924.0
60	164924.0
61	164924.0
62	139157.0
63	113390.0
64	113390.0
65	113390.0
66	88119.5
67	62849.0
68	62849.0
69	62849.0
70	46947.5
71	31046.0
72	31046.0
73	31046.0
74	21989.5
75	12933.0
76	12933.0
77	12933.0
78	8951.5
79	4970.0
80	4970.0
81	4970.0
82	3312.5
83	1655.0
84	1655.0
85	1655.0
86	1047.0
87	439.0
88	439.0
89	439.0
90	283.0
91	127.0
92	127.0
93	127.0
94	80.5
95	34.0
96	34.0
97	34.0
98	23.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3376661082016741
2	0.01054008850799777
3	0.009486079657197994
4	0.010731726480870457
5	0.012169011277415608
6	0.08135031948445552
7	0.08000885367434672
8	0.10573625153250492
9	0.08441652705041852
10	0.09169877001958061
11	0.08743482512316333
12	0.09859773704299732
13	0.0846081650232912
14	0.08882420042649031
15	0.08604544981983635
16	0.08753064410959967
17	0.10094530221068776
18	0.10247840599366925
19	0.09591480542277972
20	0.0951003440380708
21	0.08250014732169166
22	0.10362823383090536
23	0.095435710490598
24	0.09141131306027159
25	0.09150713204670792
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2087269.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.68998706616487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.46807267293242	38.710317989128704
2	14.150379285578712	14.911666030769391
3	5.180681820293324	8.18910174315512
4	2.494861016720138	5.258167788114521
5	1.4092708914464644	3.712723252151842
6	0.823302606987311	2.6027882228220744
7	0.540272953355432	1.9926882447147485
8	0.37359411837619494	1.5747735412189583
9	0.28196462259341787	1.3371041085806923
>10	1.1635873102469185	10.486435361967716
>50	0.06514377990742393	2.3595456823223313
>100	0.042099584658171964	4.15362105001332
>500	0.004207966183598334	1.5676404233787429
>1k	0.002286938143259952	2.0734941388921992
>5k	1.829550514607962E-4	0.5682720634496159
>10k+	9.14775257303981E-5	0.5016603593200681
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10408	0.4986420054147309	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6010	0.2879360542412118	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5780	0.27691687080103233	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3206	0.15359783525745843	No Hit
GTCCTACAGTGGACATTTCTAAATT	2942	0.1409497290478611	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2834	0.13577550378029857	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2390	0.11450368879143034	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2309	0.11062301984075844	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2270	0.10875454960524973	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2171	0.10401150977665075	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2126	0.10185558258183301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	4.7909493218171686E-5
9	0.0	0.0	0.0	0.0	1.4372847965451506E-4
10	0.0	0.0	0.0	0.0	1.4372847965451506E-4
11	0.0	0.0	0.0	0.0	1.4372847965451506E-4
12	0.0	0.0	0.0	0.0	1.4372847965451506E-4
13	0.0	0.0	0.0	0.0	1.4372847965451506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	120	1.00444595E-8	11.873052	18
TTAGACT	105	2.7271926E-7	11.7599745	4
CCAACGA	90	7.477516E-6	11.609206	19
GGTATCA	2425	0.0	11.473064	1
TAGGACC	590	0.0	11.430327	4
GTCCTAA	855	0.0	11.255468	1
GTGTTAC	175	2.0008883E-11	10.889308	1
ACCGATC	70	0.0014938154	10.855362	8
CCATGCG	70	0.0014944132	10.854841	9
TCGTCCC	70	0.0014944132	10.854841	9
TGTAGGA	1675	0.0	10.490816	2
TCCTAAA	910	0.0	10.333468	2
GGCGAGG	745	0.0	10.327164	19
TAACTAT	120	1.5273763E-6	10.289978	6
TGGCGAG	1495	0.0	10.229091	18
GGACCTG	1020	0.0	10.150295	6
GTCCTAC	1615	0.0	10.147621	1
GTATCAA	5015	0.0	10.145621	1
AGGACCT	1115	0.0	10.13735	5
TCGACAG	75	0.002651827	10.131671	5
>>END_MODULE