FastQCFastQC Report
Thu 26 May 2016
SRR1512850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087269
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT137290.6577494323922791No Hit
TATCAACGCAGAGTACTTTTTTTTT101110.4844128859289339No Hit
GGTATCAACGCAGAGTACTTTTTTT82360.39458258614486197No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49250.23595425409949555No Hit
GTACTGGTTCACTATCGGTCAGTCA29620.14190791891222454No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29190.13984781070384317No Hit
GTCCTACAGTGGACATTTCTAAATT28140.13481731391593513No Hit
CTGTAGGACGTGGAATATGGCAAGA24500.11737825838452062No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24420.11699498243877528No Hit
CTTTAGGACGTGAAATATGGCGAGG23690.11349758943384873No Hit
GTCCTAAAGTGTGTATTTCTCATTT22110.1059278895053776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.012.5782631
TAGCCCG550.003044421812.1039235
TACACCG550.003044421812.1039235
CCCGTAC2600.012.04840216
TAGGACC5550.011.9948794
GTTACAC1552.5465852E-1111.6585343
GTAAGAC2050.011.1347253
CTAATAC2150.011.0591973
CCGTACA2750.011.04576417
AGGACGT14600.010.9432735
TAGGACG14900.010.7867664
GCGCCAC1502.1591404E-910.75888713
TCCTATA2750.010.721392
GGCGAGG7200.010.67824319
TGTAGGA15950.010.6140572
CTAGACA1355.6004865E-810.5669164
GGACGTG14800.010.5381056
CCCCGTA3000.010.4424515
GTATCAA52150.010.3781931
TTAGGAC9350.010.3755383
Produced by FastQC (version 0.11.5)