##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512850_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087269
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.05790916264267	31.0	30.0	34.0	26.0	34.0
2	30.07330727376299	31.0	30.0	34.0	26.0	34.0
3	30.24038204946272	31.0	30.0	34.0	26.0	34.0
4	33.804264328172366	37.0	35.0	37.0	30.0	37.0
5	33.38913192310143	37.0	35.0	37.0	27.0	37.0
6	33.22843917099329	37.0	35.0	37.0	27.0	37.0
7	33.04700879474567	37.0	35.0	37.0	26.0	37.0
8	32.980678580480046	37.0	35.0	37.0	26.0	37.0
9	34.26010447144091	39.0	35.0	39.0	25.0	39.0
10	34.197056536555664	39.0	35.0	39.0	25.0	39.0
11	34.168891503682566	39.0	35.0	39.0	25.0	39.0
12	34.02964016616929	39.0	35.0	39.0	23.0	39.0
13	34.01760913423234	39.0	35.0	39.0	24.0	39.0
14	35.06851632444117	39.0	35.0	41.0	24.0	41.0
15	35.05774674946066	39.0	35.0	41.0	24.0	41.0
16	35.05113380211176	39.0	35.0	41.0	24.0	41.0
17	35.0528614184372	39.0	35.0	41.0	24.0	41.0
18	34.983776887406464	39.0	35.0	41.0	23.0	41.0
19	35.00632213672507	40.0	35.0	41.0	23.0	41.0
20	34.98391917860132	40.0	35.0	41.0	23.0	41.0
21	34.952412458576255	40.0	35.0	41.0	23.0	41.0
22	34.914232425240826	40.0	35.0	41.0	22.0	41.0
23	34.75315687628188	40.0	35.0	41.0	19.0	41.0
24	34.706919903471956	40.0	35.0	41.0	19.0	41.0
25	34.63401842311652	39.0	35.0	41.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9719316286327881
1101	2	0.7745465998712469
1101	3	0.8453596213924506
1101	4	0.8765127498727168
1101	5	0.9519071178776102
1101	6	1.1061529884144008
1101	7	1.0538338530630114
1101	8	0.7534411685833717
1101	9	1.1549639790439556
1101	10	0.9303336459763472
1101	11	1.180271273347273
1101	12	1.2970921685848609
1101	13	1.4253653497039949
1101	14	1.5286229180405755
1101	15	1.1621087701439095
1101	16	1.4399339035487202
1101	17	1.410258489654474
1101	18	1.2804322368009196
1101	19	1.1985928954564429
1101	20	1.2978742945866486
1101	21	1.377745095110491
1101	22	1.3027005398874536
1101	23	1.452321732691722
1101	24	1.5508051004420693
1101	25	1.6058245328019751
1102	1	1.0238862605590064
1102	2	1.1424508549342178
1102	3	0.8023822813486241
1102	4	0.9494426509009628
1102	5	1.1478735975173606
1102	6	1.1259304825748302
1102	7	1.0328513597454076
1102	8	1.3131387147581464
1102	9	1.377759750469778
1102	10	1.3823036895624483
1102	11	1.4701178791699903
1102	12	1.5009961346792053
1102	13	1.545555587380683
1102	14	1.540895897066271
1102	15	1.7051472752129015
1102	16	1.6118317049791173
1102	17	1.6901510896496603
1102	18	1.079083234946708
1102	19	1.3711447292031593
1102	20	1.6630949943723365
1102	21	1.4676730937619737
1102	22	1.299570528087905
1102	23	1.5061980059597175
1102	24	1.5543902378225667
1102	25	1.6143317690579053
1103	1	0.8192703761997393
1103	2	0.9795440589528717
1103	3	0.6639438024976059
1103	4	0.5758511522583021
1103	5	0.7430196565906613
1103	6	0.7256566943198806
1103	7	0.8867152544993502
1103	8	0.9258717248047859
1103	9	1.003259646059
1103	10	0.8773425535732144
1103	11	0.9597320233496731
1103	12	1.130712833442523
1103	13	1.060883553226759
1103	14	1.2286468889686333
1103	15	1.139555962189398
1103	16	1.0740512460357508
1103	17	1.1565540224323243
1103	18	1.1458536936580472
1103	19	1.2656731405352133
1103	20	1.147539181012398
1103	21	1.2845672212359247
1103	22	1.1784275781354694
1103	23	1.2325457170435001
1103	24	1.2886657430547075
1103	25	1.216458036488703
1104	1	0.7904015701561384
1104	2	0.8382549728065349
1104	3	0.6808246533788278
1104	4	0.6151026959824648
1104	5	0.6282911173924361
1104	6	0.5255571728446995
1104	7	0.5630898606362607
1104	8	0.6475363629985722
1104	9	0.6226443294300594
1104	10	0.6887141147182021
1104	11	0.7257471743529678
1104	12	0.7767439809251258
1104	13	0.6431057308114205
1104	14	0.833813514396283
1104	15	0.837660488955656
1104	16	0.8465953710274192
1104	17	0.7959114789190664
1104	18	0.4563224398061507
1104	19	0.807610485831816
1104	20	0.778732406978186
1104	21	0.6687827500556125
1104	22	0.6728907145593226
1104	23	0.7753666121706928
1104	24	0.973542861424086
1104	25	0.7594755093554042
1105	1	0.5153267033899205
1105	2	0.6127658152018327
1105	3	0.6286854438349927
1105	4	0.3955348889363819
1105	5	0.34110873870882585
1105	6	0.4381520452746983
1105	7	0.5243346868669647
1105	8	0.4620906723892091
1105	9	0.5992480010023797
1105	10	0.3558248487266624
1105	11	0.6752758654488602
1105	12	0.6571950772961586
1105	13	0.6743369281129432
1105	14	0.7597653530523587
1105	15	0.80431940594557
1105	16	0.7433406154328495
1105	17	0.4131171917345213
1105	18	0.4073134297205314
1105	19	0.672730711745757
1105	20	0.7079075136403574
1105	21	0.6718525635980228
1105	22	0.547509183306488
1105	23	0.8490232156648219
1105	24	0.732126941161745
1105	25	0.638257890284244
1106	1	0.06288805865084512
1106	2	0.42686998587231173
1106	3	-1.6116146538299283
1106	4	-0.3864054174549949
1106	5	0.03236209020401759
1106	6	0.23581951326573858
1106	7	0.43651624097172004
1106	8	0.3677104700089089
1106	9	0.7185305103652126
1106	10	0.5018264124143457
1106	11	0.4713124004458322
1106	12	0.6729917319635703
1106	13	0.4653100221203914
1106	14	0.6510690167793385
1106	15	0.7489703315894829
1106	16	0.657925955355104
1106	17	0.679905761604644
1106	18	0.8218538145585299
1106	19	0.8500153946869133
1106	20	0.9271965414904031
1106	21	0.7326170397907532
1106	22	0.7597101055600461
1106	23	0.7754959910531127
1106	24	0.6672826037603485
1106	25	0.6125969662549693
1107	1	0.7077873657202218
1107	2	0.7628040507870146
1107	3	0.6470649747518351
1107	4	0.5134485754765521
1107	5	0.6658296189369821
1107	6	0.6180188954721757
1107	7	0.5946606339849012
1107	8	0.6043202118528299
1107	9	0.6629756556765685
1107	10	0.6012258725406809
1107	11	0.7510589752164805
1107	12	0.8409482823378625
1107	13	0.7596287331024598
1107	14	0.8344791259599731
1107	15	0.8197809769333091
1107	16	0.9334769140482706
1107	17	0.9021893715227378
1107	18	0.96576205458895
1107	19	0.9899484206694922
1107	20	1.0296940601123339
1107	21	1.011227839119151
1107	22	0.9930626096647543
1107	23	1.1484128857591926
1107	24	1.0719520470968078
1107	25	1.202981649427052
1108	1	0.5864224848121715
1108	2	0.5075587042879306
1108	3	0.4475038429292937
1108	4	0.34926035894255136
1108	5	0.615695347529126
1108	6	0.6700670491855547
1108	7	0.5432908903929103
1108	8	0.5094539794691073
1108	9	0.6280893592455996
1108	10	0.6748675847311247
1108	11	0.730848346528397
1108	12	0.8938220409296989
1108	13	0.8074796458413758
1108	14	0.8584675781808144
1108	15	0.8360543211018125
1108	16	0.8182244706591177
1108	17	0.8179167808512062
1108	18	0.9091796014306155
1108	19	0.9150210323688484
1108	20	0.8697953441237445
1108	21	0.9949166448702655
1108	22	0.9821268277583144
1108	23	0.8877122211923876
1108	24	0.9205611880664506
1108	25	0.8868764402221174
1109	1	0.32795768848362883
1109	2	0.27170261844693755
1109	3	0.3086125748297377
1109	4	0.3166183139726897
1109	5	-0.06106349556169732
1109	6	0.2817158909219728
1109	7	0.3240452057878045
1109	8	0.35775092306544565
1109	9	0.5177712506554073
1109	10	0.05139725760822955
1109	11	0.4922721265416996
1109	12	0.5778741512141181
1109	13	0.5316294058950817
1109	14	0.6050511767381082
1109	15	0.5824277076923252
1109	16	0.527571565346939
1109	17	0.6945632671690589
1109	18	0.7872377720868755
1109	19	0.6441746206305439
1109	20	0.8602020185408321
1109	21	0.8125608537612479
1109	22	0.7344588314030389
1109	23	0.7059478090077178
1109	24	0.7643811825582887
1109	25	0.7464700791644816
1110	1	0.5050163856517855
1110	2	0.375758901866142
1110	3	0.4765678617441722
1110	4	0.5235687151883468
1110	5	0.554428994938327
1110	6	0.5301079595185811
1110	7	0.5708764327786184
1110	8	0.592323194264587
1110	9	0.5706179082850653
1110	10	0.6600844787069988
1110	11	0.7264978379944722
1110	12	0.9793107075001828
1110	13	0.8528953172202307
1110	14	0.8190139895545769
1110	15	0.8031748905055025
1110	16	0.769861272363741
1110	17	0.8035018102213058
1110	18	0.7796756267815113
1110	19	0.8251018240362455
1110	20	0.8361999783356708
1110	21	0.9616405392225644
1110	22	0.908544522136296
1110	23	0.9277103325129374
1110	24	1.040274751150136
1110	25	0.9076333241488896
1111	1	0.22272821701928436
1111	2	-0.013328619196400382
1111	3	-0.06075370960416038
1111	4	0.15711468935162287
1111	5	0.2435388505566607
1111	6	0.33313885124884735
1111	7	0.19366560910927433
1111	8	0.21524558498715862
1111	9	0.26318129415086133
1111	10	0.27608712184969164
1111	11	0.18830165072780858
1111	12	0.33115502639535066
1111	13	0.344822762953207
1111	14	0.15265049135521735
1111	15	0.2743415076132436
1111	16	0.13539178414225006
1111	17	0.3217869392397077
1111	18	0.133848290078177
1111	19	0.09311702210675321
1111	20	0.1978532640924371
1111	21	0.2948961641606118
1111	22	0.137179801483434
1111	23	0.24394411848445685
1111	24	0.31481771873859543
1111	25	0.11417457369459783
1112	1	-0.08865936126080243
1112	2	-0.501360468635415
1112	3	-0.1547233298017936
1112	4	0.00553118876486991
1112	5	-0.11247356458279256
1112	6	-0.15992240189058293
1112	7	-0.02825522792556967
1112	8	-0.11592172327461725
1112	9	-0.08801352044460486
1112	10	-0.45855818024837447
1112	11	-0.3940670087222813
1112	12	0.023596663175752042
1112	13	-0.1409954188649749
1112	14	-0.40415438696106065
1112	15	-0.579842021488858
1112	16	-0.6505977967919563
1112	17	-0.4838594486346892
1112	18	-0.17327427103787585
1112	19	-0.32651899057160705
1112	20	-0.3053428665955522
1112	21	-0.3172971698307663
1112	22	-0.2818360683988814
1112	23	-0.042380320256917514
1112	24	-0.03577511513762488
1112	25	-0.20131263278934597
1113	1	-0.5309967956646346
1113	2	-0.38509117627402745
1113	3	-0.35783336460162474
1113	4	-2.056770648040562
1113	5	-0.5849461824215254
1113	6	-0.25619042323111074
1113	7	-0.30003302102770846
1113	8	-0.10839520648924861
1113	9	-0.11662996916724921
1113	10	-0.20355569905340332
1113	11	-0.47633964810526663
1113	12	-0.09438327903336585
1113	13	-0.25263233598134605
1113	14	-0.22973814385533586
1113	15	-0.3302247024324245
1113	16	-0.5641800068268026
1113	17	-0.5442546640073758
1113	18	-0.47927804173308886
1113	19	-0.2861868845396174
1113	20	-0.09919392007803651
1113	21	-0.18669896521533502
1113	22	-0.07416063484330238
1113	23	-0.07316597791331247
1113	24	0.12877205663814095
1113	25	-0.13422319393604454
1114	1	-1.240807387352998
1114	2	-2.545714483570805
1114	3	-1.6678079386182745
1114	4	-1.333810069217833
1114	5	-1.193357254717668
1114	6	-0.8515352440498916
1114	7	-0.9394384978630228
1114	8	-1.1027474108740343
1114	9	-1.4553058118663316
1114	10	-1.5004265149963203
1114	11	-1.3901451284295732
1114	12	-1.4832106745491487
1114	13	-1.564080134213306
1114	14	-1.620154772361225
1114	15	-1.637597365357152
1114	16	-1.9204638881489515
1114	17	-1.991763310150084
1114	18	-1.8076072973143766
1114	19	-2.2217618911904964
1114	20	-1.644451734315716
1114	21	-1.9846020848046848
1114	22	-1.5919060522786381
1114	23	-1.5111139309690103
1114	24	-1.5475242105832763
1114	25	-2.1463046791972573
1115	1	-1.2375267754067991
1115	2	-1.3542009957877923
1115	3	-0.8360128301046181
1115	4	-0.7157596761159368
1115	5	-0.9725592981696494
1115	6	-0.9912959725852915
1115	7	-0.8232893289696079
1115	8	-1.1402736157621405
1115	9	-2.090018691973256
1115	10	-1.7685092172305659
1115	11	-1.9504211670009397
1115	12	-2.7744600335600147
1115	13	-2.68911791765872
1115	14	-2.240922293418315
1115	15	-2.3486091983221655
1115	16	-2.0420462195811098
1115	17	-2.0673996797864334
1115	18	-2.4177756735670215
1115	19	-2.9151470821473637
1115	20	-3.015313558677885
1115	21	-2.3612348241489585
1115	22	-2.2548192734578834
1115	23	-2.3527102217752542
1115	24	-2.854750810764994
1115	25	-2.7189738866608515
1116	1	-1.8097923035012364
1116	2	-1.9931244149921952
1116	3	-2.110979935830386
1116	4	-1.7386530784161423
1116	5	-2.3636042925864835
1116	6	-3.1427690566590627
1116	7	-2.5785866431429767
1116	8	-2.9683219825804095
1116	9	-4.208722609753043
1116	10	-2.857190155427581
1116	11	-3.9808194703933104
1116	12	-6.0427628915225675
1116	13	-5.0582763361458944
1116	14	-4.25094510424038
1116	15	-5.31688972361799
1116	16	-4.730177785427323
1116	17	-4.534661509208739
1116	18	-4.566740298510414
1116	19	-4.773461556471826
1116	20	-6.694759348520176
1116	21	-5.518052784788335
1116	22	-4.782107485803852
1116	23	-5.502978804438467
1116	24	-5.495416515495638
1116	25	-4.699487087967061
1201	1	0.780693302681204
1201	2	0.9624319681996383
1201	3	0.9873271153485703
1201	4	0.8748318252159564
1201	5	0.270193175410796
1201	6	0.77384493283936
1201	7	1.0442220346234237
1201	8	0.9928941913134963
1201	9	0.9849554529009055
1201	10	1.159157542097688
1201	11	0.8384631382410035
1201	12	1.3336097479506037
1201	13	1.2829148393703704
1201	14	1.1745849544747742
1201	15	1.0462105266479327
1201	16	1.0104622971858248
1201	17	0.9577952444443838
1201	18	1.2290292762632333
1201	19	1.248796366969934
1201	20	1.3612398388914784
1201	21	1.12743512090627
1201	22	1.0959990704862292
1201	23	1.1695640045778788
1201	24	0.8107876529697293
1201	25	1.0626327518853174
1202	1	0.9242122480693844
1202	2	0.9704006712495463
1202	3	0.8548247010129693
1202	4	0.8469725964289552
1202	5	0.9331093848278726
1202	6	0.9952793051631161
1202	7	0.9499081823524662
1202	8	0.852091313321516
1202	9	1.1766987427611184
1202	10	1.167076582115122
1202	11	1.1756609805554845
1202	12	1.276080610861193
1202	13	1.2608596406422024
1202	14	1.302917695252411
1202	15	1.233455077236954
1202	16	1.3244982058682169
1202	17	1.3050091386979261
1202	18	1.26299696179845
1202	19	1.3997255950312422
1202	20	1.2865891788639985
1202	21	1.5050380749712389
1202	22	1.3750420792498304
1202	23	1.5208391991828591
1202	24	1.4964698392057159
1202	25	1.6383657002852345
1203	1	0.8864816804063338
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2211	10	0.23341096719549626
2211	11	0.2159412427443428
2211	12	0.1685995047254636
2211	13	0.32963032623469246
2211	14	0.24561894379667848
2211	15	0.14007292979479757
2211	16	0.23108166249269146
2211	17	0.23275274859893358
2211	18	0.14096590723787727
2211	19	0.278331755595957
2211	20	0.27625521590701396
2211	21	0.27357584292446546
2211	22	0.23440348078946016
2211	23	0.09147270104688943
2211	24	0.3970671850392975
2211	25	0.022138654884663822
2212	1	-0.2949567450512234
2212	2	-0.1358292885404424
2212	3	-0.14803572656087738
2212	4	-0.14162605138911744
2212	5	-0.6108337509605164
2212	6	-0.19506418311081575
2212	7	-0.513944832657792
2212	8	-0.2559351379044088
2212	9	-0.19643182499238065
2212	10	-0.4685898080922968
2212	11	-0.18967380665728228
2212	12	-0.010358137370822362
2212	13	7.625219893512281E-4
2212	14	0.04273393624588806
2212	15	-0.13653533308287535
2212	16	-0.2318108039880471
2212	17	-0.3458739264452504
2212	18	-0.3377768772578378
2212	19	-0.18126628891511132
2212	20	-0.2436929206465237
2212	21	-0.15047599112720178
2212	22	-0.02352613336707776
2212	23	-0.33203937102915404
2212	24	-0.3123871005385155
2212	25	-0.1685036949354739
2213	1	-0.4053205590294944
2213	2	0.017068050200268914
2213	3	-0.13877682362727484
2213	4	-0.29009042485348857
2213	5	0.02843092444873463
2213	6	-0.00146275035275778
2213	7	-0.40111735567369067
2213	8	-0.4177568720153033
2213	9	-0.2120641330945645
2213	10	-0.3251467483841779
2213	11	-0.04873511330895042
2213	12	-0.34692751068718763
2213	13	-0.08571286358242247
2213	14	-0.25962241791769003
2213	15	-0.5001124918407598
2213	16	-1.0273645176140533
2213	17	-0.7523109768582046
2213	18	-0.4226330094031283
2213	19	-0.31824861625888445
2213	20	-0.3327200364808647
2213	21	-0.18900282573837757
2213	22	-0.4386840623804247
2213	23	-0.5508657823254453
2213	24	-0.2038726989518196
2213	25	-0.22847971695968994
2214	1	-1.3334696275851705
2214	2	-0.6783592559322464
2214	3	-0.9498678326217167
2214	4	-0.8399106765479445
2214	5	-1.2268167426477277
2214	6	-1.8062508612082127
2214	7	-1.3408240139318082
2214	8	-1.1329272231449892
2214	9	-1.064714230344407
2214	10	-1.2954406676933417
2214	11	-1.2977872005122464
2214	12	-1.008142540548974
2214	13	-1.418516500070936
2214	14	-1.0921197095954156
2214	15	-1.244217012787722
2214	16	-1.3676090158953969
2214	17	-1.4476535991385688
2214	18	-1.441643168343397
2214	19	-1.4387833967364898
2214	20	-1.7121552068944368
2214	21	-1.8725825877806557
2214	22	-2.213556139031411
2214	23	-2.1713510012858848
2214	24	-1.9512076942220133
2214	25	-2.477333167846176
2215	1	-1.6942160375801443
2215	2	-1.1501669747933754
2215	3	-1.2924524428201352
2215	4	-1.2479789560178816
2215	5	-1.7245238328163666
2215	6	-1.5512064595792232
2215	7	-2.195809383124338
2215	8	-1.797970941884543
2215	9	-1.7691823798679849
2215	10	-1.774245141448084
2215	11	-1.610580265671345
2215	12	-1.8832847927341234
2215	13	-1.9768845995817443
2215	14	-2.159326589173588
2215	15	-2.02542032318307
2215	16	-2.0496434579964387
2215	17	-2.3479575638841084
2215	18	-1.947255986612312
2215	19	-2.1020853526818257
2215	20	-2.2256074379889554
2215	21	-1.753132001936521
2215	22	-1.8767082935081092
2215	23	-2.536865104531664
2215	24	-2.4558390069526155
2215	25	-2.8180332203062974
2216	1	-1.9709486246575594
2216	2	-1.3501446920124742
2216	3	-1.3773085621590155
2216	4	-1.6504339101969734
2216	5	-2.4456951746773647
2216	6	-2.633354507244512
2216	7	-2.5767772813336123
2216	8	-2.763951218209648
2216	9	-4.651174572205004
2216	10	-2.6050729033103295
2216	11	-2.952701642275482
2216	12	-3.4823067710664475
2216	13	-3.2242455021150604
2216	14	-3.3866789199741945
2216	15	-3.3701917369200025
2216	16	-2.6420482572977946
2216	17	-2.8313198058670395
2216	18	-3.2805914923616086
2216	19	-3.529389952400223
2216	20	-2.7669952207560513
2216	21	-3.506601893337443
2216	22	-2.863457487153852
2216	23	-3.9932589747186356
2216	24	-3.308867928947052
2216	25	-3.4377884262683978
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66493.0
3	1.0
4	1062.0
5	9362.0
6	12161.0
7	9397.0
8	7476.0
9	6281.0
10	5115.0
11	4590.0
12	4101.0
13	3902.0
14	3478.0
15	3488.0
16	3712.0
17	3896.0
18	4255.0
19	5189.0
20	7161.0
21	9344.0
22	11847.0
23	14030.0
24	16412.0
25	18358.0
26	20470.0
27	22032.0
28	25095.0
29	29576.0
30	37148.0
31	46838.0
32	59108.0
33	76194.0
34	98090.0
35	129702.0
36	176686.0
37	275050.0
38	860169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15565183909716	19.066298986289667	13.663008563385306	26.11504061122787
2	13.900960771109283	20.500013180945626	39.32724453529977	26.27178151264532
3	17.590252560538147	25.35237049240344	29.35210023068084	27.705276716377575
4	11.817023639605143	16.8780331679869	36.27880925652558	35.02613393588238
5	13.126044025657515	38.67171664412897	34.159081957032384	14.043157373181131
6	32.36681511593904	36.20749660835758	17.507394499546983	13.918293776156393
7	28.338739568124556	32.04638573425299	21.107091397733456	18.507783299889
8	26.53112978032382	35.520619359787155	19.840510061001186	18.107740798887836
9	27.247028401978955	14.588624640350861	19.497487966968826	38.66685899070136
10	16.138954571622087	28.846370470427914	32.38978682364693	22.624888134303067
11	34.4760557725211	22.810033534232083	23.478849573168013	19.235061120078807
12	23.85214512977042	25.169659980558272	30.135493227255022	20.84270166241628
13	29.294736902626372	21.75847668767153	25.605183370896768	23.341603038805328
14	21.779668406432567	22.010306364246997	27.147726151201528	29.062299078118908
15	24.02409251756746	29.807590484701397	23.36744203875982	22.800874958971324
16	22.699748065934084	27.67201998606648	26.610859134454596	23.017372813544835
17	21.905656264149044	28.110897369864716	27.18039628106202	22.803050084924216
18	21.929316435512256	26.398365604875206	29.414848023580674	22.257469936031868
19	23.590069134690474	26.214360659821867	28.135519324655167	22.06005088083249
20	24.172459406900696	25.826127242735495	28.248657676480747	21.75275567388306
21	23.625986400359132	25.637474182672893	27.78842703428081	22.948112382687167
22	23.858334714731	26.541040925462976	27.482611157796498	22.118013202009525
23	22.811194915461304	26.479816449149585	28.128525839266526	22.58046279612259
24	23.43516815513477	25.848752604479824	28.29261585353876	22.423463386846638
25	22.959533706261006	26.29178428452887	28.33550842536751	22.41317358384261
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	220.0
1	220.0
2	573.5
3	927.0
4	927.0
5	927.0
6	2285.5
7	3644.0
8	3644.0
9	3644.0
10	4288.5
11	4933.0
12	4933.0
13	4933.0
14	6430.0
15	7927.0
16	7927.0
17	7927.0
18	15050.5
19	22174.0
20	22174.0
21	22174.0
22	35272.5
23	48371.0
24	48371.0
25	48371.0
26	71134.5
27	93898.0
28	93898.0
29	93898.0
30	117227.5
31	140557.0
32	140557.0
33	140557.0
34	158613.5
35	176670.0
36	176670.0
37	176670.0
38	190862.5
39	205055.0
40	205055.0
41	205055.0
42	221432.5
43	237810.0
44	237810.0
45	237810.0
46	258261.5
47	278713.0
48	278713.0
49	278713.0
50	274059.5
51	269406.0
52	269406.0
53	269406.0
54	242738.0
55	216070.0
56	216070.0
57	216070.0
58	188593.5
59	161117.0
60	161117.0
61	161117.0
62	136126.5
63	111136.0
64	111136.0
65	111136.0
66	85556.0
67	59976.0
68	59976.0
69	59976.0
70	44970.0
71	29964.0
72	29964.0
73	29964.0
74	21093.0
75	12222.0
76	12222.0
77	12222.0
78	8366.0
79	4510.0
80	4510.0
81	4510.0
82	2979.5
83	1449.0
84	1449.0
85	1449.0
86	927.5
87	406.0
88	406.0
89	406.0
90	256.5
91	107.0
92	107.0
93	107.0
94	57.0
95	7.0
96	7.0
97	7.0
98	3.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08441652705041852
2	0.04426837173359064
3	0.01930752576692319
4	0.00651569107767135
5	0.018924249821177817
6	0.06031805196167815
7	0.08211687137594627
8	0.05959940956340558
9	0.024577570020922073
10	0.05087988179769833
11	0.007090604996289409
12	2.8745695930903017E-4
13	0.006371962598016834
14	7.186423982725753E-4
15	0.01504358087050591
16	0.010300541041906912
17	0.0054616822268715726
18	0.00651569107767135
19	0.0018684702355086958
20	0.0014372847965451506
21	3.832759457453735E-4
22	5.270044253998886E-4
23	0.0
24	0.0
25	4.7909493218171686E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2087269.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.503226950302704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.67424984608864	36.19780906469714
2	14.903740105530158	15.05373937915836
3	5.619939517479774	8.514752426947672
4	2.679263795370046	5.412458700692113
5	1.5125229732859022	3.8193645493702286
6	0.9150851825189632	2.7728852790968626
7	0.5899330416049332	2.0855465599959415
8	0.4054686025044568	1.6381978282803726
9	0.29001070037562643	1.3181828597177851
>10	1.2872686282841805	11.01487508728145
>50	0.07034510290011704	2.427651731230029
>100	0.044939467750311365	4.308996422166207
>500	0.004473061926681848	1.5683509641506475
>1k	0.0024744597892282414	2.325466023522412
>5k	9.517153035493238E-5	0.3958608195140875
>10k+	1.9034306070986475E-4	1.145862304178709
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	13729	0.6577494323922791	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10111	0.4844128859289339	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8236	0.39458258614486197	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4925	0.23595425409949555	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2962	0.14190791891222454	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2919	0.13984781070384317	No Hit
GTCCTACAGTGGACATTTCTAAATT	2814	0.13481731391593513	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2450	0.11737825838452062	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2442	0.11699498243877528	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2369	0.11349758943384873	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2211	0.1059278895053776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.7909493218171686E-5	0.0	0.0	4.7909493218171686E-5	0.0
9	4.7909493218171686E-5	0.0	0.0	4.7909493218171686E-5	0.0
10	4.7909493218171686E-5	0.0	0.0	4.7909493218171686E-5	0.0
11	4.7909493218171686E-5	0.0	0.0	4.7909493218171686E-5	0.0
12	4.7909493218171686E-5	0.0	0.0	4.7909493218171686E-5	0.0
13	4.7909493218171686E-5	0.0	0.0	4.7909493218171686E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2435	0.0	12.578263	1
TAGCCCG	55	0.0030444218	12.103923	5
TACACCG	55	0.0030444218	12.103923	5
CCCGTAC	260	0.0	12.048402	16
TAGGACC	555	0.0	11.994879	4
GTTACAC	155	2.5465852E-11	11.658534	3
GTAAGAC	205	0.0	11.134725	3
CTAATAC	215	0.0	11.059197	3
CCGTACA	275	0.0	11.045764	17
AGGACGT	1460	0.0	10.943273	5
TAGGACG	1490	0.0	10.786766	4
GCGCCAC	150	2.1591404E-9	10.758887	13
TCCTATA	275	0.0	10.72139	2
GGCGAGG	720	0.0	10.678243	19
TGTAGGA	1595	0.0	10.614057	2
CTAGACA	135	5.6004865E-8	10.566916	4
GGACGTG	1480	0.0	10.538105	6
CCCCGTA	300	0.0	10.44245	15
GTATCAA	5215	0.0	10.378193	1
TTAGGAC	935	0.0	10.375538	3
>>END_MODULE