FastQCFastQC Report
Thu 26 May 2016
SRR1512849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197153
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41260.1877884698971806No Hit
GTCCTACAGTGGACATTTCTAAATT33330.1516963088141791No Hit
CTGTAGGACGTGGAATATGGCAAGA30270.13776919495365136No Hit
GTCCTAAAGTGTGTATTTCTCATTT28000.12743764316822725No Hit
GTACATGGGAAGCAGTGGTATCAAC26710.12156640889369105No Hit
TATCAACGCAGAGTACTTTTTTTTT24450.11128037055225558No Hit
GGTATCAACGCAGAGTACTTTTTTT24070.10955085968068679No Hit
CTTTAGGACGTGAAATATGGCGAGG23160.1054091362777194No Hit
GTACTGGTTCACTATCGGTCAGTCA22680.10322449096626407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG759.692212E-713.9315124
GTCCGAA500.001473754513.331061
TAGGACC5850.012.502644
GTATTAA2050.012.0769191
GTCCTAC16700.011.46083451
TAACACT2550.011.1750114
TCTAGAC1803.6379788E-1211.0818853
ACGTCTC600.00588268611.0818855
GTATAAT4000.010.712461
TCCTACA19100.010.6425882
TGTAGGA17700.010.5720642
AAGGCGT2350.010.5092656
GTAGGAC16950.010.3113373
TTAGACT1856.002665E-1110.2689294
GTCCTAG2600.010.2546621
AGGACCG1951.4551915E-1110.2294325
GCACCGT1303.8499093E-710.2294326
GTCCTAA8950.010.2137411
CCTACAG19150.010.1187813
CTGTAGG18450.010.115711
Produced by FastQC (version 0.11.5)