##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512849_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2197153
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.608222094683438	31.0	30.0	34.0	16.0	34.0
2	28.637844974837893	31.0	29.0	34.0	16.0	34.0
3	28.831164693583013	31.0	30.0	34.0	18.0	34.0
4	32.22279968668545	35.0	33.0	37.0	20.0	37.0
5	32.43187889054608	35.0	33.0	37.0	24.0	37.0
6	32.29598348408145	35.0	33.0	37.0	22.0	37.0
7	32.25400825522847	35.0	33.0	37.0	22.0	37.0
8	32.18301137881613	35.0	33.0	37.0	19.0	37.0
9	33.47374306659572	38.0	33.0	39.0	18.0	39.0
10	33.33311380682183	38.0	33.0	39.0	17.0	39.0
11	33.40247811599829	38.0	33.0	39.0	18.0	39.0
12	33.293509373266225	38.0	33.0	39.0	17.0	39.0
13	33.26661320354113	38.0	33.0	39.0	17.0	39.0
14	34.07955294874777	39.0	33.0	41.0	17.0	41.0
15	34.26071284066244	39.0	34.0	41.0	17.0	41.0
16	34.197548827960546	39.0	33.0	41.0	17.0	41.0
17	33.92263033116037	39.0	33.0	41.0	11.0	41.0
18	33.9188754720313	39.0	33.0	41.0	11.0	41.0
19	34.02440385353228	39.0	34.0	41.0	11.0	41.0
20	33.99765878844122	39.0	34.0	41.0	10.0	41.0
21	33.88798322192401	39.0	34.0	41.0	10.0	41.0
22	33.88593284127232	39.0	34.0	41.0	8.0	41.0
23	33.542800615159706	39.0	33.0	41.0	2.0	41.0
24	33.60534792069556	39.0	33.0	41.0	2.0	41.0
25	33.30708830928024	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7017647803413212
1101	2	0.3220126486785446
1101	3	0.7191371124402721
1101	4	0.8453042324658355
1101	5	0.7605639179120942
1101	6	0.6919191908915607
1101	7	0.41495491414240604
1101	8	0.7529217642094892
1101	9	0.7213752024136966
1101	10	0.8097803375661599
1101	11	0.7640345629297229
1101	12	0.7024177039558666
1101	13	1.1836268357445903
1101	14	1.2901914792907903
1101	15	1.0872634393882592
1101	16	0.7490663661807844
1101	17	0.27654722876660287
1101	18	0.7435798868416157
1101	19	0.0967389241063259
1101	20	-0.36127804477050773
1101	21	0.710154230595144
1101	22	0.756860997017192
1101	23	0.1939473297739056
1101	24	0.7564460184152892
1101	25	0.4145631160366037
1102	1	0.9091242697096966
1102	2	0.8151058137734246
1102	3	0.8676499100146096
1102	4	1.0088747430813427
1102	5	0.9588167780476127
1102	6	0.9781281905124786
1102	7	0.8093790219543493
1102	8	0.8001561025602442
1102	9	1.0787996112312825
1102	10	1.0565507941761396
1102	11	0.78005733836644
1102	12	1.0436988276434036
1102	13	1.0561847014373456
1102	14	1.2586769410407825
1102	15	1.2379225808542884
1102	16	1.1662578528603404
1102	17	1.3637348279789379
1102	18	1.2670639671171884
1102	19	1.143947566853818
1102	20	1.315403938385714
1102	21	1.5345297960998607
1102	22	1.3165168764845774
1102	23	1.1340704860248394
1102	24	0.5426577935194885
1102	25	1.1583385575936163
1103	1	0.9253226744365541
1103	2	0.9950109097446216
1103	3	1.1799558496537976
1103	4	1.3439008947891793
1103	5	1.0619757747074914
1103	6	0.6583329217148517
1103	7	0.46974559066202204
1103	8	0.8290834877377122
1103	9	0.9202227510016385
1103	10	1.0606836225775567
1103	11	0.8153958485966228
1103	12	0.9530008982297389
1103	13	1.121077968885409
1103	14	1.2371323403883423
1103	15	1.0471856195555063
1103	16	1.1524908380444288
1103	17	0.8272194995163105
1103	18	1.1674557686283293
1103	19	1.129974358863869
1103	20	1.2412552524189522
1103	21	0.998558569357364
1103	22	1.147943440164994
1103	23	0.6212482359194169
1103	24	0.8202625943034363
1103	25	1.1297747689078292
1104	1	0.8541741560807665
1104	2	0.8360362221836013
1104	3	0.8586496879340828
1104	4	1.0462951415183355
1104	5	0.8019649125711865
1104	6	0.7956321552629859
1104	7	0.906357194027251
1104	8	0.626182748136074
1104	9	1.068561596767438
1104	10	1.0437171284032445
1104	11	0.7208749216943247
1104	12	1.092937861225245
1104	13	1.1090935135381486
1104	14	1.3650121286783943
1104	15	1.0842730007636021
1104	16	1.131498844293212
1104	17	1.3517905061556377
1104	18	1.08897479974204
1104	19	0.7327268119980204
1104	20	0.9754095517094541
1104	21	1.1068544751049387
1104	22	0.8694399943861768
1104	23	1.339673795705913
1104	24	1.142901605238798
1104	25	0.6966419986155685
1105	1	0.613619035691503
1105	2	0.7171654888373133
1105	3	0.8165717216517123
1105	4	0.8918118660722385
1105	5	0.6454988474460208
1105	6	0.8904574208156291
1105	7	0.907878336362387
1105	8	0.751355043628152
1105	9	0.6190833071801265
1105	10	0.6064927337229307
1105	11	0.6987819674751705
1105	12	0.8637001005302736
1105	13	0.8331630841470812
1105	14	0.9942085317085017
1105	15	0.9334048102727621
1105	16	0.9673905506308529
1105	17	1.265350793891642
1105	18	1.0816213327148247
1105	19	0.7908389695322668
1105	20	0.9249795129560425
1105	21	0.8146305257110669
1105	22	0.8448828688585763
1105	23	0.9259208878505234
1105	24	1.0300978996460017
1105	25	1.2383616273816074
1106	1	0.6985506118742038
1106	2	0.6634209357330114
1106	3	0.5856391174282507
1106	4	0.7873598221110569
1106	5	0.5840682553207159
1106	6	0.7525174387400426
1106	7	0.835579562861291
1106	8	0.7824164866136201
1106	9	0.9147598593141026
1106	10	0.6223805654799293
1106	11	0.5338932254574971
1106	12	0.8031861824166455
1106	13	0.7254514177737832
1106	14	1.0796582882746506
1106	15	0.7849631604259386
1106	16	0.9057579383560537
1106	17	1.0541938043021375
1106	18	1.0275490927013706
1106	19	1.0133286741746517
1106	20	1.0870554862255943
1106	21	1.062837889384518
1106	22	0.980653062661851
1106	23	1.2605596821498963
1106	24	0.9449028566016722
1106	25	1.0733713986221716
1107	1	1.0055475307852575
1107	2	0.9838982198195332
1107	3	0.9744226883280938
1107	4	1.083324544591619
1107	5	0.9667152080794637
1107	6	0.9698978172015202
1107	7	0.7566027121517536
1107	8	0.9210120921077021
1107	9	0.8485790810069958
1107	10	1.0943505011376757
1107	11	1.1219899729157632
1107	12	1.0746628907760751
1107	13	0.8626083382032377
1107	14	1.3718282732804425
1107	15	1.013820626847128
1107	16	1.0477636197069202
1107	17	1.3855567591107203
1107	18	1.2794412103015347
1107	19	1.1199675034873806
1107	20	1.2615344532066999
1107	21	1.1450026232530917
1107	22	0.9965393344176974
1107	23	1.3177327988580032
1107	24	1.0813871253333645
1107	25	1.3246571680403108
1108	1	0.6771684212751055
1108	2	0.6942534820922575
1108	3	0.7588221346514246
1108	4	0.711287825798621
1108	5	0.7393745888830239
1108	6	0.7702691638222774
1108	7	0.7425280718071576
1108	8	0.6811097805687609
1108	9	0.7527200361604116
1108	10	0.8904626145476229
1108	11	0.576861369481577
1108	12	0.7584146006299264
1108	13	0.7835344215523676
1108	14	0.7774895560734478
1108	15	0.8768048951723841
1108	16	0.6510766284585188
1108	17	0.993931546844216
1108	18	1.0381358706649308
1108	19	0.917264843760023
1108	20	1.0411938075087974
1108	21	0.9606891232051851
1108	22	0.9084382863868541
1108	23	1.2658650370694389
1108	24	1.1772181194031504
1108	25	1.285389097611656
1109	1	0.8124651212087777
1109	2	0.7950770060151058
1109	3	0.8021130949421114
1109	4	0.8419158455793792
1109	5	0.6646071909164988
1109	6	0.9401668538378374
1109	7	0.8286739400439842
1109	8	0.8018501951659616
1109	9	1.0393547952582125
1109	10	1.1500843142610648
1109	11	1.0562944785951558
1109	12	0.941666618004156
1109	13	1.012462147140532
1109	14	1.206787901684656
1109	15	1.0981635318309912
1109	16	0.6576370710557313
1109	17	1.0910762767446158
1109	18	1.0998558744887532
1109	19	1.10835642323525
1109	20	0.9783917478670219
1109	21	0.9934627064918331
1109	22	1.037355702020598
1109	23	1.397129680222669
1109	24	1.1681116727126266
1109	25	0.7172717018147949
1110	1	0.3797319279483773
1110	2	0.3819124042166955
1110	3	0.3358411280444038
1110	4	0.3522607148043875
1110	5	0.20019038538442402
1110	6	0.21784107961286736
1110	7	0.13617718023686365
1110	8	0.11533833324245535
1110	9	0.35893369093607674
1110	10	0.25747106851356705
1110	11	0.3977346537668254
1110	12	0.16402459290373628
1110	13	0.25239557224578846
1110	14	0.23537437257122207
1110	15	0.1709837474145104
1110	16	0.27386291048706113
1110	17	0.3171879647844804
1110	18	-0.3214098535282517
1110	19	0.15798168788454348
1110	20	0.253023814908822
1110	21	0.3937969494942237
1110	22	0.44760316420492785
1110	23	0.42752003290055995
1110	24	0.4237984728510895
1110	25	0.6778486574020093
1111	1	0.2797582548969224
1111	2	0.21955246458780664
1111	3	0.2668216762478437
1111	4	0.22271162911318498
1111	5	0.30378367221351255
1111	6	0.20596686213619364
1111	7	0.10516716996734488
1111	8	0.2655023441028206
1111	9	0.31013349639800936
1111	10	0.419478347613385
1111	11	0.09309898055057175
1111	12	-0.2291865110394795
1111	13	0.18866691264921087
1111	14	0.1758546484615664
1111	15	0.26576236993228974
1111	16	0.403735234835068
1111	17	-0.05196183555274558
1111	18	0.24697730937125328
1111	19	0.29187733268376803
1111	20	0.4165867554079128
1111	21	0.2827489158761196
1111	22	6.91355463175114E-4
1111	23	0.35508659888171934
1111	24	0.38842333393122175
1111	25	0.32060659618281306
1112	1	0.17343399142515992
1112	2	0.15993485214840675
1112	3	0.07832797057192309
1112	4	0.17211606283557757
1112	5	0.1411821699869762
1112	6	-0.028832085561546705
1112	7	-0.14067361081605867
1112	8	0.04663193742717198
1112	9	-0.11726644705692735
1112	10	0.0034383031979459133
1112	11	-0.07325319963968013
1112	12	-0.015419824797881176
1112	13	-0.023119334207791553
1112	14	-0.033255164349931476
1112	15	-0.39949056342275924
1112	16	-0.01590563031714254
1112	17	0.3335888344613238
1112	18	-0.008045792682963793
1112	19	0.19126046584417367
1112	20	-0.06121898859555586
1112	21	0.028286936978581423
1112	22	-0.1647179262607139
1112	23	0.12889210054922273
1112	24	0.13037175355453456
1112	25	0.0519451241392801
1113	1	-0.12525106964380228
1113	2	-0.02396735245813275
1113	3	0.04768079267242342
1113	4	0.11845320092968592
1113	5	-0.08942120496624284
1113	6	-0.48461875245502384
1113	7	-0.377985640891243
1113	8	-0.14783613320189204
1113	9	-0.34179907387284203
1113	10	-0.21646524526597233
1113	11	-0.28495964335076707
1113	12	-0.36098892288246276
1113	13	-0.17825831287618144
1113	14	0.059696388740938744
1113	15	-0.19120117479280907
1113	16	-0.17057043146027695
1113	17	-0.206750276891718
1113	18	-0.4329747723720061
1113	19	-0.8110530101535147
1113	20	-0.422461461293139
1113	21	-0.29131509411725887
1113	22	-0.7784546152683021
1113	23	-4.72094884797594
1113	24	-2.183980303057581
1113	25	-1.2188846652663585
1114	1	-0.8741777452299644
1114	2	-1.0642520673472795
1114	3	-0.8914332950074808
1114	4	-0.7638916981011512
1114	5	-0.710079194995604
1114	6	-1.3933061576737842
1114	7	-1.1056424179643685
1114	8	-1.0323908613031918
1114	9	-1.395083080203392
1114	10	-1.0335449534950882
1114	11	-1.228057045180762
1114	12	-2.141145348809193
1114	13	-1.28210000233328
1114	14	-3.266046670961604
1114	15	-1.974344216080837
1114	16	-2.013868461019925
1114	17	-1.8989278666333398
1114	18	-1.6797709858042609
1114	19	-1.816332302506943
1114	20	-1.417842167509015
1114	21	-1.3272774449583693
1114	22	-1.476512231820621
1114	23	-5.7648699078306045
1114	24	-2.8865046583449754
1114	25	-2.6919330702089006
1115	1	-1.7410066389383907
1115	2	-1.6345696207282785
1115	3	-1.4295587252204527
1115	4	-1.5493166879835805
1115	5	-1.4184254130993104
1115	6	-1.5541643006757084
1115	7	-2.204875644414642
1115	8	-2.3533251541627784
1115	9	-3.6878540322236226
1115	10	-4.483733382452442
1115	11	-3.2481225240272593
1115	12	-3.6128511713418057
1115	13	-2.7880189545333742
1115	14	-3.6761895794953006
1115	15	-3.1792546971929774
1115	16	-3.142543301300659
1115	17	-2.994821083882673
1115	18	-5.886835385443142
1115	19	-3.6867236190364636
1115	20	-4.103342044714555
1115	21	-3.782641054907831
1115	22	-3.2811446959075674
1115	23	-3.716819658955643
1115	24	-3.523274161044725
1115	25	-3.7291322670293887
1116	1	-1.4879289721643012
1116	2	-1.4184048097059936
1116	3	-1.8656597582131518
1116	4	-1.67387928609363
1116	5	-1.7552503379898958
1116	6	-4.096294012123874
1116	7	-2.5217712778460424
1116	8	-2.850684369490299
1116	9	-3.9029818655032464
1116	10	-3.374012734033812
1116	11	-2.8838200280036403
1116	12	-2.692658173930351
1116	13	-4.114285505460504
1116	14	-7.170008343161403
1116	15	-5.0945077994630665
1116	16	-3.935546343795089
1116	17	-4.938180874442622
1116	18	-3.501268608234824
1116	19	-3.255320086554889
1116	20	-4.17359906272349
1116	21	-4.875964113758268
1116	22	-4.552730812707473
1116	23	-3.450347133234782
1116	24	-4.501850911258703
1116	25	-4.1986131544120475
1201	1	0.5692272882819474
1201	2	0.6092725587230241
1201	3	0.8976678328260448
1201	4	0.7450610780477405
1201	5	0.38556907297595444
1201	6	0.7709681060695175
1201	7	0.8728766539177073
1201	8	0.8622648979707606
1201	9	1.207201314314112
1201	10	1.1644976530667677
1201	11	1.216921577006083
1201	12	0.9879735853510851
1201	13	1.2033942996150273
1201	14	1.2392014738508834
1201	15	1.1546173320764268
1201	16	1.2655034530498455
1201	17	1.4590928180482337
1201	18	1.2301393665857105
1201	19	1.3839727730740634
1201	20	1.4495750494885655
1201	21	1.5374643004397441
1201	22	1.3532276805732621
1201	23	1.6463570146552513
1201	24	1.5031564018523653
1201	25	1.6145808741457373
1202	1	1.0385600035620932
1202	2	0.808274381463697
1202	3	0.8762843852053841
1202	4	1.1179144802640977
1202	5	0.9972036394161847
1202	6	1.275718544398643
1202	7	1.3135742992543271
1202	8	1.2825383954068243
1202	9	1.4729365933401155
1202	10	1.460083904195912
1202	11	1.2316682512987285
1202	12	1.5261073513216488
1202	13	1.4399117906359393
1202	14	1.852096000101639
1202	15	1.5386091314790065
1202	16	1.2662173650052182
1202	17	1.6115311331092173
1202	18	1.5246022811248139
1202	19	1.523348573176584
1202	20	1.3617286328575275
1202	21	1.595714300283987
1202	22	1.5342527356698028
1202	23	1.667629828619198
1202	24	1.6408662887185486
1202	25	1.921309616707454
1203	1	0.9523850113224128
1203	2	0.9699027531495261
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2211	15	-0.3753029623502684
2211	16	-0.4830076878501046
2211	17	0.09141224008398297
2211	18	-0.31905308043732106
2211	19	-0.2707929056969718
2211	20	-0.3210457365240629
2211	21	-0.19765550635498386
2211	22	-0.15262182114005896
2211	23	0.07609143788243244
2211	24	-0.3109469428144891
2211	25	0.060411508812606485
2212	1	-0.00679879304774289
2212	2	0.06350063364840608
2212	3	-0.25796109988628757
2212	4	-0.006587041909000391
2212	5	-0.3137232187419414
2212	6	-0.34250045235897275
2212	7	-0.21466133444889124
2212	8	-0.1154685340071282
2212	9	0.07546679159160163
2212	10	-0.26056020061777474
2212	11	-0.5331333524243576
2212	12	-0.04629979331702572
2212	13	-0.0630517528746708
2212	14	-0.06490423062972894
2212	15	-0.28026394665485554
2212	16	-0.3619370923603924
2212	17	-0.06654256827956573
2212	18	0.1470547780211504
2212	19	-0.2204004284665615
2212	20	-0.015553575886166016
2212	21	0.12463745739896126
2212	22	0.2729377910967088
2212	23	0.3048836957048522
2212	24	0.21343690699452367
2212	25	0.36188041060082554
2213	1	-0.15665197075361448
2213	2	-0.06680706922101365
2213	3	-0.257336728907287
2213	4	-0.09542721173114188
2213	5	-0.11483278134915054
2213	6	0.08821601270533819
2213	7	-0.05937371966946614
2213	8	0.1326194619725527
2213	9	-0.3515827515903567
2213	10	0.13473739395180928
2213	11	-0.005944173344111903
2213	12	-0.10997421948754038
2213	13	-0.13200209268128305
2213	14	-0.08191637411685804
2213	15	-0.0457770079715516
2213	16	-0.11856666933362447
2213	17	-0.1096219676389083
2213	18	-0.10534953638654088
2213	19	-0.017125782341601337
2213	20	0.1283620324921273
2213	21	-0.13051231385382778
2213	22	-0.2471797208479245
2213	23	-0.07504930003133126
2213	24	0.12357508532265626
2213	25	0.28022646751843183
2214	1	-2.5759686141441414
2214	2	-1.324645843945973
2214	3	-1.655210702857012
2214	4	-3.2371331271959995
2214	5	-1.7433030045150844
2214	6	-1.450936533894449
2214	7	-1.3359995462099654
2214	8	-1.3143966199404638
2214	9	-1.0072735898164282
2214	10	-1.1850208429518005
2214	11	-1.091257225558671
2214	12	-1.018375184259817
2214	13	-1.4170784051705922
2214	14	-1.2240895343517053
2214	15	-1.5990961737356741
2214	16	-1.421505519587285
2214	17	-3.5700572195912557
2214	18	-3.3491465636908764
2214	19	-2.139322848122827
2214	20	-1.9976504796209689
2214	21	-1.8062763294155886
2214	22	-2.8717600377834565
2214	23	-1.7931913296157802
2214	24	-2.973682189658817
2214	25	-2.861346560184927
2215	1	-1.9215781587340501
2215	2	-3.394577203742326
2215	3	-2.1812808676214743
2215	4	-3.7164363399631455
2215	5	-2.306252680021405
2215	6	-2.065768307699976
2215	7	-2.3615826207875656
2215	8	-1.6892107703824486
2215	9	-4.008405995825157
2215	10	-2.3006115753004615
2215	11	-1.861879707935305
2215	12	-1.7936362128488277
2215	13	-2.0231402827581526
2215	14	-1.6908327787290176
2215	15	-1.9163057029401003
2215	16	-1.9006192112623452
2215	17	-2.33136921606658
2215	18	-1.8904812187764755
2215	19	-2.0221921694834606
2215	20	-1.9531205408831376
2215	21	-2.5722543211913553
2215	22	-2.403418801910121
2215	23	-2.4288447228126024
2215	24	-2.298938672538977
2215	25	-3.203820964347397
2216	1	-2.9318279977486092
2216	2	-3.0865885285486243
2216	3	-4.4111692099388335
2216	4	-4.6259591155079
2216	5	-4.658698490245538
2216	6	-3.6331171826194826
2216	7	-5.345791929121418
2216	8	-3.2049027935539627
2216	9	-3.934662169097418
2216	10	-2.9100365586826094
2216	11	-3.279595051924833
2216	12	-3.5874701784394887
2216	13	-3.1840763871182602
2216	14	-4.194999250655233
2216	15	-3.529701606379998
2216	16	-3.9749384960196217
2216	17	-3.697808912738857
2216	18	-3.1251556322182417
2216	19	-3.863314796246513
2216	20	-4.372624089054135
2216	21	-4.2296206708205695
2216	22	-3.7433126164341246
2216	23	-3.750573148167291
2216	24	-4.069568988953261
2216	25	-4.2655050018621985
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	122801.0
3	6.0
4	1090.0
5	6593.0
6	7375.0
7	6858.0
8	5921.0
9	5605.0
10	5137.0
11	4799.0
12	4662.0
13	4528.0
14	4380.0
15	4577.0
16	4778.0
17	5186.0
18	5663.0
19	7024.0
20	8913.0
21	11434.0
22	13874.0
23	17113.0
24	19871.0
25	22482.0
26	25073.0
27	27691.0
28	30412.0
29	36280.0
30	44387.0
31	55085.0
32	68769.0
33	86596.0
34	112321.0
35	146553.0
36	196324.0
37	297041.0
38	769951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59529414558428	17.467210221286766	11.835099642293322	25.102395990835625
2	15.893592993473144	19.585074215163665	39.80412604356184	24.717206747801356
3	19.86748938076138	24.584424995311696	28.108027652806882	27.44005797112004
4	12.916129549112574	15.601489727399187	36.35266228233083	35.1297184411574
5	13.974493839220239	38.06651338875433	33.82185926320088	14.137133508824556
6	33.220888963386194	34.754966621968286	17.809375364388995	14.214769050256528
7	29.747672031031087	30.825251562759053	20.898930345856662	18.528146060353198
8	27.20963809751179	34.69394356757771	19.977465666578432	18.118952668332064
9	28.258016732433656	14.34045268762495	18.885275740795315	38.51625483914608
10	16.941639193709516	27.651332072090373	32.36017490976859	23.04685382443152
11	35.932683613438684	21.68304631930415	23.176870639842758	19.207399427414416
12	24.89776802635799	23.834081698107706	29.848273901507422	21.419876374026884
13	29.95475479998597	20.9223862390457	25.055264007769075	24.067594953199254
14	22.58694021199186	21.071593373580416	26.21245740279227	30.129009011635453
15	24.66436938157784	29.1463528511464	22.510812651314485	23.678465115961274
16	23.83745136213357	26.76633690246371	25.9986990424114	23.397512692991317
17	23.191625261619457	27.325873287343587	26.29174180016055	23.19075965087641
18	23.132866380979543	26.102739844606372	28.018097972520454	22.746295801893627
19	24.719934946460665	25.871446370691288	26.322721133061368	23.085897549786687
20	25.34507083638365	25.635942124585842	26.436487331724013	22.582499707306493
21	24.941662935432724	25.442202465028508	25.738044147140126	23.87809045239864
22	25.08593632898226	26.438912425356698	25.567954280278403	22.90719696538264
23	23.9838057639235	26.267936524134182	26.20944265915661	23.538815052785704
24	24.458112662274655	25.667661869225466	26.588626629579654	23.285598838920222
25	24.119623273240784	25.86951223678666	26.351194068945095	23.659670421027457
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	262.0
1	262.0
2	585.5
3	909.0
4	909.0
5	909.0
6	1487.5
7	2066.0
8	2066.0
9	2066.0
10	2354.0
11	2642.0
12	2642.0
13	2642.0
14	3785.0
15	4928.0
16	4928.0
17	4928.0
18	9853.5
19	14779.0
20	14779.0
21	14779.0
22	25123.0
23	35467.0
24	35467.0
25	35467.0
26	55234.0
27	75001.0
28	75001.0
29	75001.0
30	98790.0
31	122579.0
32	122579.0
33	122579.0
34	144771.0
35	166963.0
36	166963.0
37	166963.0
38	187529.0
39	208095.0
40	208095.0
41	208095.0
42	228613.5
43	249132.0
44	249132.0
45	249132.0
46	274331.0
47	299530.0
48	299530.0
49	299530.0
50	298296.5
51	297063.0
52	297063.0
53	297063.0
54	273022.5
55	248982.0
56	248982.0
57	248982.0
58	219917.0
59	190852.0
60	190852.0
61	190852.0
62	162536.5
63	134221.0
64	134221.0
65	134221.0
66	105707.5
67	77194.0
68	77194.0
69	77194.0
70	57998.5
71	38803.0
72	38803.0
73	38803.0
74	28017.0
75	17231.0
76	17231.0
77	17231.0
78	12140.0
79	7049.0
80	7049.0
81	7049.0
82	4710.5
83	2372.0
84	2372.0
85	2372.0
86	1581.0
87	790.0
88	790.0
89	790.0
90	492.5
91	195.0
92	195.0
93	195.0
94	114.0
95	33.0
96	33.0
97	33.0
98	24.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3354340821963696
2	0.010468092117390094
3	0.008966148465764559
4	0.010968740001265274
5	0.012379656764913505
6	0.07723631444874345
7	0.0763260455689704
8	0.10377065229412791
9	0.08083187652384699
10	0.08938840399371367
11	0.08410884449102998
12	0.0976263373556598
13	0.08110495718777891
14	0.08556527469866686
15	0.08228830673148388
16	0.08406333104704133
17	0.09880968689936476
18	0.10063022465891086
19	0.09275639884887397
20	0.09211921063303284
21	0.07837415054845975
22	0.10076676499087683
23	0.09334807362072646
24	0.08897878299781581
25	0.08774992001012219
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2197153.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.10062740686771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89905127938088	41.269847176730444
2	13.677234523366177	15.072484068566952
3	4.847246527706352	8.012589746171429
4	2.3077329927706036	5.086301431567551
5	1.2206276876472653	3.362867570977923
6	0.750302518085696	2.480528369488473
7	0.48359802621384396	1.8652588259973961
8	0.33513290905460064	1.4772826842877786
9	0.2510166272988883	1.244805628835216
>10	1.1264298212871473	10.673531478735393
>50	0.06112147119222412	2.306142364337179
>100	0.03450838523333819	3.589585053963995
>500	0.003998153842015501	1.5132828100906175
>1k	0.001999076921007621	2.0454927902497686
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4126	0.1877884698971806	No Hit
GTCCTACAGTGGACATTTCTAAATT	3333	0.1516963088141791	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3027	0.13776919495365136	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2800	0.12743764316822725	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2671	0.12156640889369105	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2445	0.11128037055225558	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2407	0.10955085968068679	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2316	0.1054091362777194	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2268	0.10322449096626407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.551344398865259E-5
2	0.0	0.0	0.0	0.0	4.551344398865259E-5
3	0.0	0.0	0.0	0.0	4.551344398865259E-5
4	0.0	0.0	0.0	0.0	4.551344398865259E-5
5	0.0	0.0	0.0	0.0	4.551344398865259E-5
6	0.0	0.0	0.0	0.0	1.3654033196595775E-4
7	0.0	0.0	0.0	0.0	1.3654033196595775E-4
8	0.0	0.0	0.0	0.0	1.3654033196595775E-4
9	0.0	0.0	0.0	0.0	1.3654033196595775E-4
10	0.0	0.0	0.0	4.551344398865259E-5	1.3654033196595775E-4
11	0.0	0.0	0.0	4.551344398865259E-5	1.3654033196595775E-4
12	0.0	0.0	0.0	4.551344398865259E-5	1.8205377595461035E-4
13	0.0	0.0	0.0	4.551344398865259E-5	1.8205377595461035E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGACG	75	9.692212E-7	13.931512	4
GTCCGAA	50	0.0014737545	13.33106	1
TAGGACC	585	0.0	12.50264	4
GTATTAA	205	0.0	12.076919	1
GTCCTAC	1670	0.0	11.4608345	1
TAACACT	255	0.0	11.175011	4
TCTAGAC	180	3.6379788E-12	11.081885	3
ACGTCTC	60	0.005882686	11.081885	5
GTATAAT	400	0.0	10.71246	1
TCCTACA	1910	0.0	10.642588	2
TGTAGGA	1770	0.0	10.572064	2
AAGGCGT	235	0.0	10.509265	6
GTAGGAC	1695	0.0	10.311337	3
TTAGACT	185	6.002665E-11	10.268929	4
GTCCTAG	260	0.0	10.254662	1
AGGACCG	195	1.4551915E-11	10.229432	5
GCACCGT	130	3.8499093E-7	10.229432	6
GTCCTAA	895	0.0	10.213741	1
CCTACAG	1915	0.0	10.118781	3
CTGTAGG	1845	0.0	10.11571	1
>>END_MODULE