FastQCFastQC Report
Thu 26 May 2016
SRR1512849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197153
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55700.2535098830167949No Hit
TATCAACGCAGAGTACTTTTTTTTT39980.18196274906663307No Hit
GGTATCAACGCAGAGTACTTTTTTT33480.15237901047400887No Hit
GTCCTACAGTGGACATTTCTAAATT31160.14181989146864146No Hit
CTGTAGGACGTGGAATATGGCAAGA29730.13531146897826415No Hit
GTCCTAAAGTGTGTATTTCTCATTT27210.1238420810931237No Hit
GTACATGGGAAGCAGTGGTATCAAC25030.11392015030359742No Hit
CTTTAGGACGTGAAATATGGCGAGG24780.1127823142038811No Hit
CCCATGTACTCTGCGTTGATACCAC24090.1096418865686641No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG400.00525738814.259237
CGAAATT400.00530686714.23911119
CGCGCCT1950.012.1710112
CGTTATT550.003076779212.08689610
TTAGACT1552.5465852E-1111.6550944
TCGCGCG2150.011.4866519
TAGAAAT4350.011.3660084
GTTGACG600.005822964511.0970871
GGTATCA19250.010.9200291
TAGTACT1502.1045707E-910.775854
TAGGACC6050.010.6867934
GTTACGG803.7955554E-410.680305517
GTCCTAA9300.010.5345631
GTCCTAT1909.094947E-1210.513031
ATAGTAC1553.601599E-910.4303833
TATCGCG2200.010.3703497
CGCCTTT2750.010.3566614
CCGTACA1856.002665E-1110.26335617
TCGAACT1856.002665E-1110.26242219
ATCGCGC2350.010.1062498
Produced by FastQC (version 0.11.5)