##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512849_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2197153 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.140511379953967 31.0 30.0 34.0 26.0 34.0 2 30.12554792497382 31.0 30.0 34.0 26.0 34.0 3 30.315844640769214 31.0 30.0 34.0 26.0 34.0 4 33.92657589161975 37.0 35.0 37.0 30.0 37.0 5 33.48563709491328 37.0 35.0 37.0 28.0 37.0 6 33.359007315375855 37.0 35.0 37.0 27.0 37.0 7 33.19778959407925 37.0 35.0 37.0 26.0 37.0 8 33.13884877384506 37.0 35.0 37.0 26.0 37.0 9 34.46056237321661 39.0 35.0 39.0 26.0 39.0 10 34.3832145508301 39.0 35.0 39.0 26.0 39.0 11 34.376008862377816 39.0 35.0 39.0 26.0 39.0 12 34.22601111529329 39.0 35.0 39.0 25.0 39.0 13 34.21819964290152 39.0 35.0 39.0 25.0 39.0 14 35.27940475697414 39.0 35.0 41.0 25.0 41.0 15 35.26508804803307 39.0 35.0 41.0 25.0 41.0 16 35.25306476153459 39.0 35.0 41.0 25.0 41.0 17 35.22874101166373 39.0 35.0 41.0 25.0 41.0 18 35.1373418237146 39.0 35.0 41.0 25.0 41.0 19 35.14253763848034 40.0 35.0 41.0 25.0 41.0 20 35.11587313218515 40.0 35.0 41.0 25.0 41.0 21 35.07780932870856 40.0 35.0 41.0 24.0 41.0 22 35.03538260649122 40.0 35.0 41.0 24.0 41.0 23 34.877197901102015 39.0 35.0 41.0 23.0 41.0 24 34.82451745508847 39.0 35.0 41.0 23.0 41.0 25 34.76009909187025 39.0 35.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6473854636340199 1101 2 0.4184507687216943 1101 3 0.3969014330116458 1101 4 0.5252671821384283 1101 5 0.5034021046173933 1101 6 0.5481845285489229 1101 7 0.6476333574933832 1101 8 0.3685665963945155 1101 9 0.6916726153137063 1101 10 0.42892667776705196 1101 11 0.7867624124293116 1101 12 0.9030715309685533 1101 13 0.9617666842673245 1101 14 1.0751109232006826 1101 15 0.8204193784122822 1101 16 1.0113597109148245 1101 17 0.9886803775756547 1101 18 0.7377368917288649 1101 19 0.5648273388428393 1101 20 0.6909710621024487 1101 21 0.982448038584522 1101 22 0.9000692556527241 1101 23 0.9790290864424804 1101 24 1.0069215835438214 1101 25 1.1384892548071406 1102 1 0.8138614575838226 1102 2 0.824052195355911 1102 3 0.514768508133951 1102 4 0.7016706321178674 1102 5 0.9547303345233829 1102 6 0.9240836284507381 1102 7 0.7965259486183172 1102 8 0.956699471492577 1102 9 1.1284494340476101 1102 10 1.0748757345772546 1102 11 1.0663344257451826 1102 12 1.1413886712968093 1102 13 1.1467482577443064 1102 14 1.117922752775982 1102 15 1.3262202279966715 1102 16 1.2268074062161816 1102 17 1.243995830947263 1102 18 0.7108766598739464 1102 19 1.0782029299536262 1102 20 1.244721518938043 1102 21 1.1823471184312382 1102 22 0.907029361930455 1102 23 1.2626013769485525 1102 24 1.1498145646689224 1102 25 1.2515165138132716 1103 1 0.8322840246336725 1103 2 0.9779267823230562 1103 3 0.744730100345226 1103 4 0.6516762007127852 1103 5 0.8440975249138205 1103 6 0.803668711236611 1103 7 0.9314514518520696 1103 8 1.0489298851236697 1103 9 1.0737223865718022 1103 10 1.0120178489124072 1103 11 1.1789608957766617 1103 12 1.344879266577884 1103 13 1.2708887890813614 1103 14 1.292847835348077 1103 15 1.2929129618940323 1103 16 1.2959657346446676 1103 17 1.30792588663666 1103 18 1.2594956655745975 1103 19 1.3603307055878702 1103 20 1.268441664459388 1103 21 1.4080924395184908 1103 22 1.3571078567064916 1103 23 1.456383825220101 1103 24 1.3768949693178527 1103 25 1.3612985179764436 1104 1 0.748538275919227 1104 2 0.8763708931737995 1104 3 0.7630894499188763 1104 4 0.5257176273547159 1104 5 0.7596235655634302 1104 6 0.70364094566348 1104 7 0.5836510344531263 1104 8 0.6638524059529303 1104 9 0.679653641860483 1104 10 0.6808035475064287 1104 11 0.7976602464894142 1104 12 0.783706013469768 1104 13 0.5996438540100826 1104 14 0.9547150243736553 1104 15 0.7852545455690958 1104 16 0.84341943834837 1104 17 0.8083932229316559 1104 18 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2215 5 -1.473015323325459 2215 6 -1.2028984061883747 2215 7 -1.803791095176507 2215 8 -1.5250943533063257 2215 9 -1.364327839620998 2215 10 -1.4291501561972737 2215 11 -1.0990411423994786 2215 12 -1.4619537087524535 2215 13 -1.683920960804734 2215 14 -1.8190118274781995 2215 15 -1.7419445285049804 2215 16 -1.8244046357257062 2215 17 -1.9948475178090845 2215 18 -1.5863392950450717 2215 19 -1.8987088700158523 2215 20 -1.8143628008143224 2215 21 -1.4880760130949326 2215 22 -1.6561407967140624 2215 23 -2.2215340008880844 2215 24 -2.113917982254101 2215 25 -2.4484589960182177 2216 1 -1.327082524178401 2216 2 -0.6880223085669179 2216 3 -0.7251548805374917 2216 4 -1.0823031150997409 2216 5 -1.8364514731250061 2216 6 -2.0450732805158687 2216 7 -1.9082764870462228 2216 8 -2.1447633334895393 2216 9 -4.187815420373372 2216 10 -2.1557094105231087 2216 11 -2.2149832772221814 2216 12 -2.562894175298645 2216 13 -2.3982417515548207 2216 14 -2.6448255829049643 2216 15 -2.5847347826542446 2216 16 -1.8773376865555562 2216 17 -2.2422069995917653 2216 18 -2.613760048664645 2216 19 -2.789874923899241 2216 20 -1.9770126211337953 2216 21 -2.5761238504373196 2216 22 -2.1581798915085244 2216 23 -3.2312237988253933 2216 24 -2.5974789671734015 2216 25 -2.6785576580215036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65244.0 3 2.0 4 1140.0 5 9175.0 6 10604.0 7 8149.0 8 6652.0 9 5643.0 10 4910.0 11 4416.0 12 4153.0 13 3831.0 14 3920.0 15 3907.0 16 3919.0 17 4177.0 18 4575.0 19 5468.0 20 7062.0 21 9282.0 22 11561.0 23 14332.0 24 16650.0 25 18999.0 26 21755.0 27 23631.0 28 26824.0 29 32084.0 30 40343.0 31 51315.0 32 64922.0 33 83031.0 34 108109.0 35 141546.0 36 192063.0 37 294347.0 38 889412.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.57451648772639 18.327252750156134 12.802842879522458 27.29538788259502 2 14.354833862582266 19.973417648896227 38.73988363505227 26.93186485346923 3 17.945286296446014 24.582199837577797 28.76602826729013 28.706485598686054 4 12.198260362947824 16.289684616820132 35.62864984683729 35.88340517339475 5 13.640872496511788 38.14138121760744 33.975922810962885 14.241823474917886 6 33.431110953233876 35.23255088466659 17.376954812348487 13.959383349751048 7 29.526327392914865 30.92610036823185 20.90754301352491 18.640029225328377 8 27.110442766600162 35.06528579242165 19.620394688604232 18.203876752373958 9 27.726586529093726 14.229366335299442 18.82109994008803 39.222947195518806 10 16.41986984938101 28.069174448367107 32.39581051796595 23.115145184285936 11 35.74398221565161 21.636485952585996 23.133717617028402 19.485814214733992 12 24.444051004394794 24.09987693114334 29.87710409513441 21.578967969327454 13 29.743197604027273 20.882923232378403 25.183566831435307 24.190312332159014 14 22.28056082125131 20.99984752871353 26.29665450689193 30.42293714314323 15 24.619943600936796 29.19955663690429 22.44787411037192 23.732625651786996 16 23.77145588201825 26.734609344773435 25.938551173217416 23.555383599990897 17 22.980607955722142 27.37794345561177 26.31237099090365 23.32907759776244 18 22.97087675602456 26.097720172161416 28.130894379993155 22.800508691820877 19 24.54161354762099 25.774779494326406 26.554986575540855 23.12862038251175 20 25.162588804277625 25.519740276645607 26.61586349686201 22.70180742221476 21 24.635731990500403 25.32863086931865 25.98775866510464 24.047878475076303 22 24.828344561597063 26.30872764246514 25.86087148075629 23.002056315181505 23 23.632173089448028 26.10960638608235 26.5462168542655 23.712003670204123 24 24.285199983797217 25.556071880292357 26.833497712721872 23.325230423188554 25 23.842148309333314 25.765639230075678 26.739218196655578 23.652994263935433 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 111.0 1 111.0 2 282.0 3 453.0 4 453.0 5 453.0 6 1044.0 7 1635.0 8 1635.0 9 1635.0 10 1972.5 11 2310.0 12 2310.0 13 2310.0 14 3524.5 15 4739.0 16 4739.0 17 4739.0 18 9944.5 19 15150.0 20 15150.0 21 15150.0 22 26168.5 23 37187.0 24 37187.0 25 37187.0 26 57393.5 27 77600.0 28 77600.0 29 77600.0 30 101336.5 31 125073.0 32 125073.0 33 125073.0 34 146149.0 35 167225.0 36 167225.0 37 167225.0 38 188217.5 39 209210.0 40 209210.0 41 209210.0 42 229789.0 43 250368.0 44 250368.0 45 250368.0 46 275358.5 47 300349.0 48 300349.0 49 300349.0 50 298739.0 51 297129.0 52 297129.0 53 297129.0 54 272104.5 55 247080.0 56 247080.0 57 247080.0 58 218611.5 59 190143.0 60 190143.0 61 190143.0 62 161500.5 63 132858.0 64 132858.0 65 132858.0 66 103852.5 67 74847.0 68 74847.0 69 74847.0 70 56213.0 71 37579.0 72 37579.0 73 37579.0 74 26991.0 75 16403.0 76 16403.0 77 16403.0 78 11503.5 79 6604.0 80 6604.0 81 6604.0 82 4442.5 83 2281.0 84 2281.0 85 2281.0 86 1453.5 87 626.0 88 626.0 89 626.0 90 397.0 91 168.0 92 168.0 93 168.0 94 95.5 95 23.0 96 23.0 97 23.0 98 12.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08711273179428106 2 0.04364739278511783 3 0.018614998591358907 4 0.00650842249037732 5 0.02130029178668941 6 0.061625203160635605 7 0.0817876588476087 8 0.06126109560872638 9 0.027171526061225595 10 0.0492455463957221 11 0.0075097182581276775 12 4.0962099589787325E-4 13 0.006053288050490794 14 8.192419917957465E-4 15 0.014018140748504998 16 0.009239229129696476 17 0.005370586390661005 18 0.006599449378354626 19 0.002002591535500714 20 0.002093618423478019 21 3.185941079205681E-4 22 8.647554357843993E-4 23 0.0 24 0.0 25 9.102688797730518E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2197153.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.349720222536476 #Duplication Level Percentage of deduplicated Percentage of total 1 73.30856751252517 39.109915667081445 2 14.456421729635839 15.424921093901375 3 5.235831361337255 8.379904147791747 4 2.4384569491664507 5.203639840509201 5 1.3100014719957167 3.494410601104122 6 0.7858341182872992 2.5154418211170633 7 0.49875761564147847 1.8626005477332581 8 0.37848215726529183 1.6153533759460297 9 0.2647520492767498 1.2712002981532013 >10 1.212058492242598 11.123588702349018 >50 0.06857415229500126 2.507258774363025 >100 0.03566510222240233 3.5780227089027985 >500 0.004369632383751743 1.57935610160992 >1k 0.002141976658701768 2.0797841694619597 >5k 8.567906634807072E-5 0.25460214997589264 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5570 0.2535098830167949 No Hit TATCAACGCAGAGTACTTTTTTTTT 3998 0.18196274906663307 No Hit GGTATCAACGCAGAGTACTTTTTTT 3348 0.15237901047400887 No Hit GTCCTACAGTGGACATTTCTAAATT 3116 0.14181989146864146 No Hit CTGTAGGACGTGGAATATGGCAAGA 2973 0.13531146897826415 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2721 0.1238420810931237 No Hit GTACATGGGAAGCAGTGGTATCAAC 2503 0.11392015030359742 No Hit CTTTAGGACGTGAAATATGGCGAGG 2478 0.1127823142038811 No Hit CCCATGTACTCTGCGTTGATACCAC 2409 0.1096418865686641 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3654033196595775E-4 2 0.0 0.0 0.0 0.0 1.3654033196595775E-4 3 0.0 0.0 0.0 0.0 1.3654033196595775E-4 4 0.0 0.0 0.0 4.551344398865259E-5 1.3654033196595775E-4 5 0.0 0.0 0.0 4.551344398865259E-5 1.3654033196595775E-4 6 0.0 0.0 0.0 4.551344398865259E-5 1.3654033196595775E-4 7 0.0 0.0 0.0 4.551344398865259E-5 1.3654033196595775E-4 8 0.0 0.0 0.0 4.551344398865259E-5 1.3654033196595775E-4 9 0.0 0.0 0.0 4.551344398865259E-5 1.3654033196595775E-4 10 0.0 0.0 0.0 9.102688797730518E-5 1.3654033196595775E-4 11 0.0 0.0 0.0 1.3654033196595775E-4 1.3654033196595775E-4 12 0.0 0.0 0.0 1.3654033196595775E-4 1.8205377595461035E-4 13 0.0 0.0 0.0 1.3654033196595775E-4 2.2756721994326293E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTCG 40 0.005257388 14.25923 7 CGAAATT 40 0.005306867 14.239111 19 CGCGCCT 195 0.0 12.17101 12 CGTTATT 55 0.0030767792 12.086896 10 TTAGACT 155 2.5465852E-11 11.655094 4 TCGCGCG 215 0.0 11.486651 9 TAGAAAT 435 0.0 11.366008 4 GTTGACG 60 0.0058229645 11.097087 1 GGTATCA 1925 0.0 10.920029 1 TAGTACT 150 2.1045707E-9 10.77585 4 TAGGACC 605 0.0 10.686793 4 GTTACGG 80 3.7955554E-4 10.6803055 17 GTCCTAA 930 0.0 10.534563 1 GTCCTAT 190 9.094947E-12 10.51303 1 ATAGTAC 155 3.601599E-9 10.430383 3 TATCGCG 220 0.0 10.370349 7 CGCCTTT 275 0.0 10.35666 14 CCGTACA 185 6.002665E-11 10.263356 17 TCGAACT 185 6.002665E-11 10.262422 19 ATCGCGC 235 0.0 10.106249 8 >>END_MODULE