FastQCFastQC Report
Thu 26 May 2016
SRR1512848_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512848_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952611
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT91450.4683472540101433No Hit
GGTATCAACGCAGAGTACTTTTTTT51640.2644663990933166No Hit
TATCAACGCAGAGTACTTTTTTTTT51480.2636469834493404No Hit
GTCCTACAGTGGACATTTCTAAATT37460.1918456876459264No Hit
GTCCTAAAGTGTGTATTTCTCATTT34860.17853018343131324No Hit
CTGTAGGACGTGGAATATGGCAAGA32670.1673144318043891No Hit
CTTTAGGACGTGAAATATGGCGAGG27990.1433465242180854No Hit
GTACTGGTTCACTATCGGTCAGTCA27080.13868609774297083No Hit
GTACATGGGAAGCAGTGGTATCAAC25250.1293140313149931No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25020.12813612132677732No Hit
GTCCTACAGTGTGCATTTCTCATTT22570.11558881927839185No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20120.10304151723000637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC400.005284991614.24786615
TCTATAC1650.012.0894093
GTCCTAG3300.011.8297651
CCGTACA2250.011.82105817
TAGGACC6950.011.6172624
CCGAATG1900.011.49886818
CGAATGG2000.011.39858419
GTCCTAC19150.011.3363261
GGTATCA23250.011.221061
TAAGACG600.00588199911.0819594
TCCGAAT2100.010.85607417
CCCGTAC2450.010.85607316
GTCCTAA9800.010.59026051
TCCTACA21950.010.5590542
TCGAACT1803.45608E-1110.55424519
AATGTCC5700.010.4986968
GACGTGG10150.010.4814587
TGTAGGA21650.010.44212152
TTCCGAA2200.010.36261616
TGTCCCG3600.010.29038911
Produced by FastQC (version 0.11.5)