##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512848_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1952611
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.521806442757928	31.0	30.0	34.0	16.0	34.0
2	28.541482660908905	31.0	28.0	34.0	16.0	34.0
3	28.737006500526732	31.0	30.0	34.0	16.0	34.0
4	32.11657672726416	35.0	33.0	37.0	19.0	37.0
5	32.33761153655285	35.0	33.0	37.0	22.0	37.0
6	32.17801446371038	35.0	33.0	37.0	20.0	37.0
7	32.11821453428256	35.0	33.0	37.0	19.0	37.0
8	32.033997555068574	35.0	33.0	37.0	18.0	37.0
9	33.27139967971091	38.0	33.0	39.0	17.0	39.0
10	33.13887405120631	38.0	33.0	39.0	17.0	39.0
11	33.19365505981478	38.0	33.0	39.0	17.0	39.0
12	33.081402798611705	38.0	33.0	39.0	16.0	39.0
13	33.04325541544117	38.0	33.0	39.0	15.0	39.0
14	33.86025429540241	39.0	33.0	41.0	11.0	41.0
15	34.032728997224744	39.0	33.0	41.0	16.0	41.0
16	33.96771604789689	39.0	33.0	41.0	11.0	41.0
17	33.73189539544743	39.0	33.0	41.0	10.0	41.0
18	33.728094331128936	39.0	33.0	41.0	10.0	41.0
19	33.84537421944258	39.0	33.0	41.0	10.0	41.0
20	33.82972338064264	39.0	33.0	41.0	9.0	41.0
21	33.726394555802464	39.0	33.0	41.0	2.0	41.0
22	33.729269168308484	39.0	34.0	41.0	2.0	41.0
23	33.391999225652214	39.0	33.0	41.0	2.0	41.0
24	33.45506145361262	39.0	33.0	41.0	2.0	41.0
25	33.171158003309415	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.729955848392013
1101	2	0.37272977272387564
1101	3	0.7109775383388985
1101	4	0.8809140375864786
1101	5	0.9069357612689544
1101	6	0.7893812902485635
1101	7	0.5829175352864908
1101	8	0.9080145423490364
1101	9	0.9441059927043653
1101	10	0.9743150621576362
1101	11	0.8948918770398109
1101	12	0.8492472803708395
1101	13	1.2246506592079527
1101	14	1.1572613332856463
1101	15	1.0654156431697785
1101	16	1.0048298456394704
1101	17	0.26627080951406157
1101	18	0.7210335250018289
1101	19	0.21507563689516473
1101	20	-0.4706612759890092
1101	21	0.6872003003637133
1101	22	0.8470621686239284
1101	23	0.363092255849061
1101	24	0.9984147483592167
1101	25	0.6127767433539049
1102	1	1.009843339481673
1102	2	1.014162076270523
1102	3	0.9329401562282627
1102	4	1.0784731632153424
1102	5	0.9618065732939556
1102	6	0.8966156889871186
1102	7	0.9117768607901411
1102	8	0.9225848069901943
1102	9	1.1441668638698417
1102	10	1.0593141328791518
1102	11	0.7862044246738904
1102	12	1.035358699719282
1102	13	0.9238295149487712
1102	14	1.140018536792148
1102	15	1.1212168752971508
1102	16	1.0908155041554295
1102	17	1.1867734327279393
1102	18	1.1459446270713087
1102	19	1.0420359920941635
1102	20	1.2234510712679736
1102	21	1.3829798691145712
1102	22	1.2066276407453458
1102	23	1.0593679725465321
1102	24	0.5108666233783339
1102	25	0.8782056202146862
1103	1	0.7668178393268654
1103	2	0.8041721551227212
1103	3	0.8825426015027311
1103	4	0.9453855430760782
1103	5	0.8334863161185382
1103	6	0.3802128510985412
1103	7	0.4391089952011882
1103	8	0.5472331999732063
1103	9	0.7321545847976196
1103	10	0.9463585259412142
1103	11	0.6126459310999834
1103	12	0.7599385828572949
1103	13	0.8552320564005171
1103	14	1.0197936400509775
1103	15	0.8600836961109692
1103	16	0.9788822749043575
1103	17	0.5705023445412749
1103	18	1.013416943165197
1103	19	0.8464351146411531
1103	20	1.0612019721065593
1103	21	0.9466907312187445
1103	22	0.7987160711452717
1103	23	0.21013113269488315
1103	24	0.44225862485393463
1103	25	0.8192410411220479
1104	1	1.0825432483872177
1104	2	0.9207273241531375
1104	3	1.041930920031355
1104	4	1.2577860382343644
1104	5	0.9842372119068656
1104	6	0.9664957048288727
1104	7	1.1585750683740912
1104	8	0.9687171084167829
1104	9	1.4418058201914263
1104	10	1.3216856763226943
1104	11	1.1280976542551642
1104	12	1.3857398973328472
1104	13	1.4361371343835998
1104	14	1.6702882544432072
1104	15	1.4073828426373538
1104	16	1.3586630216013091
1104	17	1.638701043119113
1104	18	1.5315789834957982
1104	19	1.0812199840311862
1104	20	1.1846517198499598
1104	21	1.2968618392985718
1104	22	1.1289631499072001
1104	23	1.4496495934400997
1104	24	1.3052977567170245
1104	25	1.0140747301600328
1105	1	0.9330691692033213
1105	2	0.9362597975752536
1105	3	1.1114315116909417
1105	4	1.2241685423827917
1105	5	1.0287990806960607
1105	6	1.2784840915817384
1105	7	1.111934467487039
1105	8	1.3032004714341383
1105	9	1.0804643238063676
1105	10	1.1921021225661192
1105	11	1.053816989154484
1105	12	1.309067493812286
1105	13	1.2798976304119378
1105	14	1.550726646226721
1105	15	1.57958088300294
1105	16	1.3604231104980826
1105	17	1.679015151291459
1105	18	1.538759835634501
1105	19	1.2588482533412773
1105	20	1.4437214050759977
1105	21	1.346974757685281
1105	22	1.5199087906108275
1105	23	1.6319794453092982
1105	24	1.616814134615943
1105	25	1.8722140538153766
1106	1	0.9589451358888574
1106	2	0.9855526284993985
1106	3	0.8857013006061756
1106	4	0.8961711006737545
1106	5	0.831400718337207
1106	6	1.1108538792732148
1106	7	1.0062545720343152
1106	8	1.0726180737899256
1106	9	1.1977264114413657
1106	10	0.967497765613679
1106	11	0.6827446775015744
1106	12	1.0080799239375153
1106	13	0.9800504064990037
1106	14	1.2664323757808376
1106	15	0.9465661766943541
1106	16	1.090150168160747
1106	17	1.291231183892279
1106	18	1.2241216064458982
1106	19	1.290206318108801
1106	20	1.4483346374689035
1106	21	1.4511768085689951
1106	22	1.1786835289688966
1106	23	1.5414039006902414
1106	24	1.2304275016218469
1106	25	1.470021987480159
1107	1	1.0617902141478162
1107	2	0.9735095181118005
1107	3	1.028661696719091
1107	4	1.216900389314283
1107	5	1.058962870133243
1107	6	1.097935465967339
1107	7	0.9177979928767854
1107	8	1.0640019197704618
1107	9	1.1262255340229075
1107	10	1.3258812635885846
1107	11	1.1892167360342185
1107	12	1.3292817174887261
1107	13	1.0664872836720534
1107	14	1.3200381478085887
1107	15	1.0688392775514686
1107	16	1.1932940658449809
1107	17	1.550725549691677
1107	18	1.4352704798702192
1107	19	1.2631114881608596
1107	20	1.4976766529609762
1107	21	1.19050641644386
1107	22	1.185878889175882
1107	23	1.4088104759869182
1107	24	1.2900315579501083
1107	25	1.5831855913799373
1108	1	0.6787511379198854
1108	2	0.7250676079542693
1108	3	0.67107257773527
1108	4	0.670737500608162
1108	5	0.547103672719409
1108	6	0.638337636619454
1108	7	0.558810991074715
1108	8	0.7164619649549664
1108	9	0.7895229211177721
1108	10	0.8778300748932821
1108	11	0.5638434128507299
1108	12	0.6375526857310021
1108	13	0.5728248490559125
1108	14	0.7203437656668825
1108	15	0.6950871065932205
1108	16	0.5473925084694358
1108	17	0.7738621991403392
1108	18	0.923149951125815
1108	19	0.7664453249047938
1108	20	0.9606726059478632
1108	21	0.8348523330015141
1108	22	0.7988274943543416
1108	23	1.1374510020374018
1108	24	0.8838201173318083
1108	25	0.9786429569233803
1109	1	0.7356662911691743
1109	2	0.6790484850966187
1109	3	0.8212748821500462
1109	4	0.8560699789645057
1109	5	0.677401180902109
1109	6	0.8084884576806246
1109	7	0.7346339714276198
1109	8	0.7087995005111551
1109	9	0.9396968996303201
1109	10	0.9024689914808874
1109	11	0.8503666875056552
1109	12	0.7358000513194582
1109	13	0.9307999041990485
1109	14	1.0909692097985513
1109	15	0.9733747278044831
1109	16	0.660423224010465
1109	17	1.122368397791945
1109	18	1.1140766925427599
1109	19	0.8219481856156605
1109	20	0.8300710048365048
1109	21	0.8787067822002399
1109	22	0.8366961467351786
1109	23	1.1551906114601564
1109	24	0.8711593627051428
1109	25	0.37569810991814734
1110	1	0.8101955636807716
1110	2	0.7385841586233894
1110	3	0.7859304482746303
1110	4	0.7455700663207381
1110	5	0.6885839025544982
1110	6	0.7762366387808228
1110	7	0.6245727811963988
1110	8	0.6617873390931877
1110	9	0.8027763754111845
1110	10	0.7487752532369427
1110	11	0.8097747494131298
1110	12	0.8225678809308761
1110	13	0.871094728929755
1110	14	0.9610129696516694
1110	15	0.7577323427647684
1110	16	0.8282016477362077
1110	17	0.8934644171063582
1110	18	0.3401232559690044
1110	19	0.7692569495402992
1110	20	0.8310624725336027
1110	21	0.9126583183927934
1110	22	0.9102946411428405
1110	23	1.0971839274312245
1110	24	0.9673442963603804
1110	25	1.226952003911613
1111	1	0.44207638266927773
1111	2	0.4056239855203856
1111	3	0.41626106078904
1111	4	0.3577588948786712
1111	5	0.37565603388522817
1111	6	0.27059047391646374
1111	7	0.23957859701032902
1111	8	0.38323880371323327
1111	9	0.46104270413419357
1111	10	0.4088701348127515
1111	11	0.13516268198016235
1111	12	-0.2398756751980784
1111	13	0.20289427890258338
1111	14	0.3184483226893562
1111	15	0.2653416622891456
1111	16	0.38375511512955995
1111	17	-0.20444138194584127
1111	18	0.1274803367859576
1111	19	0.12861228307341577
1111	20	0.23694533340612622
1111	21	0.08367016843114783
1111	22	-0.26658533975894017
1111	23	0.14776119140822175
1111	24	0.12977718593102594
1111	25	0.30687398565656565
1112	1	0.4515743480776777
1112	2	0.5538840327395071
1112	3	0.5293778770099138
1112	4	0.5832107705383578
1112	5	0.5819236487123973
1112	6	0.3394429561586989
1112	7	0.1447365725992782
1112	8	0.3301424902657004
1112	9	0.12339957180427774
1112	10	0.3278964251162364
1112	11	0.2882203376813166
1112	12	0.2946531809880213
1112	13	0.3259666791944369
1112	14	0.45990136820125826
1112	15	0.12015915623495488
1112	16	0.37007705319823003
1112	17	0.6734991114362998
1112	18	0.3978541329058132
1112	19	0.3510322579964793
1112	20	0.20929330574661265
1112	21	0.18435335807977538
1112	22	0.1603169097778263
1112	23	0.23259207388766612
1112	24	0.3130098438332496
1112	25	0.36459799565900397
1113	1	-0.2446038078233741
1113	2	-0.041144486544663295
1113	3	-0.07683754365234208
1113	4	0.0343229089902195
1113	5	-0.10323938282639489
1113	6	-0.43734970284099717
1113	7	-0.4603003635539267
1113	8	-0.28351687406925663
1113	9	-0.28179957999807925
1113	10	-0.24215358676893572
1113	11	-0.25728316859672873
1113	12	-0.410066735461605
1113	13	-0.32128655718486243
1113	14	0.12750127475784012
1113	15	-0.16661755555166735
1113	16	-0.18965036800290136
1113	17	-0.02307490961386094
1113	18	-0.3806658166707777
1113	19	-0.7655264321085724
1113	20	-0.49145352502887363
1113	21	-0.302898187145594
1113	22	-0.7342847876411582
1113	23	-4.1920703965606485
1113	24	-1.8214598248295673
1113	25	-1.029753202142743
1114	1	-0.6659990174009636
1114	2	-1.0793729447498315
1114	3	-0.8043857540646968
1114	4	-0.8098000935314253
1114	5	-0.6329204426011756
1114	6	-1.3273623246315118
1114	7	-1.2216755149530343
1114	8	-1.1822644040873698
1114	9	-1.3266413791158023
1114	10	-1.0670704223499072
1114	11	-1.193528969695322
1114	12	-2.1028166639995973
1114	13	-1.3517064283417142
1114	14	-3.319754665538433
1114	15	-2.0111527867278696
1114	16	-1.8923286067234315
1114	17	-1.7877999612373614
1114	18	-1.7892589399374472
1114	19	-1.6988367312618209
1114	20	-1.2692807220313185
1114	21	-1.2229757295251389
1114	22	-1.3537313489761686
1114	23	-5.368489073339212
1114	24	-2.7835379345659206
1114	25	-2.8010078471731923
1115	1	-2.01335412523801
1115	2	-1.812087398464218
1115	3	-1.4497072682210828
1115	4	-1.4544309846293224
1115	5	-1.3907092284359166
1115	6	-1.6735846997091706
1115	7	-2.065611345253913
1115	8	-2.3345248771502973
1115	9	-3.5279754742609164
1115	10	-4.456484751807615
1115	11	-3.0109720556986943
1115	12	-3.3026591242818384
1115	13	-2.5795820829081464
1115	14	-3.624449238184212
1115	15	-2.9487110320460594
1115	16	-3.1626845133534154
1115	17	-3.22973215259265
1115	18	-5.74324125554941
1115	19	-3.6735177688050875
1115	20	-3.9554538382113122
1115	21	-3.4629974744439274
1115	22	-3.075651003720111
1115	23	-3.6078795499523295
1115	24	-3.2817045938363556
1115	25	-3.410918981459158
1116	1	-1.8631262996211433
1116	2	-1.6322757890113841
1116	3	-2.019656972820073
1116	4	-1.8328814325576523
1116	5	-1.8127882819942407
1116	6	-4.018703342795245
1116	7	-2.834319110632144
1116	8	-3.1715656058061263
1116	9	-4.3145146969508055
1116	10	-3.572143177493988
1116	11	-3.169056244987029
1116	12	-2.9622808951433264
1116	13	-4.518728509135123
1116	14	-7.328207154159035
1116	15	-5.419970009919943
1116	16	-4.194398611511502
1116	17	-5.210322568470936
1116	18	-3.83980239197448
1116	19	-3.5745606019517844
1116	20	-4.425055046845518
1116	21	-5.186051303072727
1116	22	-4.818559207373045
1116	23	-3.778175819790537
1116	24	-4.831407986121178
1116	25	-4.727909125105139
1201	1	0.822098092443813
1201	2	0.813483792717669
1201	3	1.1179690765061139
1201	4	1.123753589810775
1201	5	0.6573809914907756
1201	6	1.1223252162837412
1201	7	1.2276843105801092
1201	8	1.3100053452317155
1201	9	1.605998836933594
1201	10	1.551112386865455
1201	11	1.637842922641859
1201	12	1.5646907941663954
1201	13	1.7454757925368796
1201	14	1.752764387975489
1201	15	1.6832540804603013
1201	16	1.5880634829285256
1201	17	1.9020142828060784
1201	18	1.6388746838742918
1201	19	1.8438708428857495
1201	20	1.8126307037834621
1201	21	2.0109063997032877
1201	22	1.8606021531223433
1201	23	2.177702668255634
1201	24	1.9822985235455661
1201	25	2.1781516572753006
1202	1	0.8824971762635201
1202	2	0.7725544721303343
1202	3	0.9139885891419688
1202	4	1.197311169992176
1202	5	0.9534603174673393
1202	6	1.2058166355638562
1202	7	1.1943525452225217
1202	8	1.0069271456757747
1202	9	1.3478782302125083
1202	10	1.1643318040192625
1202	11	1.217773008467148
1202	12	1.3497815158179165
1202	13	1.220170692416147
1202	14	1.6744009072930055
1202	15	1.4109680737886166
1202	16	1.0883173743866692
1202	17	1.4894093586385395
1202	18	1.4255535566291115
1202	19	1.5055364344473645
1202	20	1.2771380375788581
1202	21	1.636454821151922
1202	22	1.4645376187491905
1202	23	1.7220833446979142
1202	24	1.6946563325417827
1202	25	1.9690541961789165
1203	1	1.013349901757838
1203	2	1.0876990063358107
1203	3	1.081984233595282
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2211	20	-0.16503356283934068
2211	21	-0.06269126832493299
2211	22	0.06425092063800975
2211	23	0.291164036374731
2211	24	-0.01088183037951751
2211	25	0.269613975202212
2212	1	0.1575736350090331
2212	2	0.3080136165372167
2212	3	-0.02279644153911775
2212	4	0.26025057238118876
2212	5	0.09067302549162548
2212	6	-0.06298898752071835
2212	7	0.14394595212547756
2212	8	0.1319249169807506
2212	9	0.11106485158575907
2212	10	-0.11703606295660052
2212	11	-0.33650708295101595
2212	12	0.24860688540412923
2212	13	0.18526368412244665
2212	14	0.23568485084567214
2212	15	-0.08949955653940123
2212	16	-0.19595934840513252
2212	17	0.09305939665559038
2212	18	0.26399590295280717
2212	19	0.11118496570833258
2212	20	0.24111873903173375
2212	21	0.4072793517993034
2212	22	0.4389290953332292
2212	23	0.39153638126718704
2212	24	0.40450651043120445
2212	25	0.6101324899976177
2213	1	-0.27518771957313604
2213	2	-0.20173342896738333
2213	3	-0.4458679885860306
2213	4	-0.16772633085387412
2213	5	-0.3517134328343623
2213	6	-0.20205630725325818
2213	7	-0.3261461719714198
2213	8	-0.12106441892193232
2213	9	-0.6888452059964649
2213	10	-0.30008070401024156
2213	11	-0.45215090434034977
2213	12	-0.3498420071033834
2213	13	-0.4875159700191958
2213	14	-0.5519042153749254
2213	15	-0.31817084344389457
2213	16	-0.47973700875751035
2213	17	-0.25738245558168416
2213	18	-0.42121880372126697
2213	19	-0.3790732770777083
2213	20	-0.27435065171985684
2213	21	-0.4643711382282305
2213	22	-0.707581026869839
2213	23	-0.4087774528596668
2213	24	-0.20290914548066752
2213	25	-0.009367013732600071
2214	1	-2.6777465336379755
2214	2	-1.368114439146055
2214	3	-1.5771773938690927
2214	4	-3.228054238297716
2214	5	-1.7246303469668014
2214	6	-1.4750773189869157
2214	7	-1.3720013812788672
2214	8	-1.293031928376024
2214	9	-1.053393385692253
2214	10	-1.1754269581163754
2214	11	-1.226320553194519
2214	12	-1.2086625510457445
2214	13	-1.454643588926949
2214	14	-1.328872729090108
2214	15	-1.6799577370807626
2214	16	-1.5173545774954036
2214	17	-3.6797497257408764
2214	18	-3.5454000069772285
2214	19	-2.2379392827318085
2214	20	-2.1440675364803568
2214	21	-2.0290747084303966
2214	22	-2.89952577307254
2214	23	-1.8519277443113502
2214	24	-2.9884697067438317
2214	25	-3.0295393794909664
2215	1	-1.6641686409477678
2215	2	-3.132739379297103
2215	3	-1.9061395594364434
2215	4	-3.441282079893
2215	5	-2.152383989713492
2215	6	-2.0813878035339286
2215	7	-2.1702900448015967
2215	8	-1.404653921567398
2215	9	-3.9424772116055955
2215	10	-2.279670613028003
2215	11	-1.853096273676961
2215	12	-1.6127344236596883
2215	13	-1.7707753484229372
2215	14	-1.4418292920808895
2215	15	-1.6266187014913243
2215	16	-1.7674312336279243
2215	17	-2.078460121675711
2215	18	-1.7492003261349964
2215	19	-1.816607274550023
2215	20	-1.7455295275168936
2215	21	-2.2734332403486484
2215	22	-2.033207911506537
2215	23	-2.035185539242317
2215	24	-1.9516148454697202
2215	25	-2.916281029359613
2216	1	-3.7408753359278357
2216	2	-3.6422875374464674
2216	3	-5.084206886701182
2216	4	-5.719135738185631
2216	5	-5.229794920531187
2216	6	-4.230507696275492
2216	7	-6.036502739254967
2216	8	-3.9430178962444593
2216	9	-4.6339902036542995
2216	10	-3.599185344980228
2216	11	-3.7854478064753607
2216	12	-4.318987721923683
2216	13	-3.736585244073055
2216	14	-4.688170442515371
2216	15	-4.301052496900809
2216	16	-4.477959843549808
2216	17	-4.2427247220383535
2216	18	-3.77729610811922
2216	19	-4.546545560713234
2216	20	-5.211208202980675
2216	21	-4.66193421481055
2216	22	-4.299140480129466
2216	23	-4.372020085533602
2216	24	-4.725266104836848
2216	25	-4.752788633367665
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	113941.0
3	6.0
4	1043.0
5	6003.0
6	7720.0
7	6989.0
8	6213.0
9	5749.0
10	5127.0
11	4684.0
12	4370.0
13	4375.0
14	4123.0
15	4232.0
16	4419.0
17	4580.0
18	5153.0
19	6203.0
20	8080.0
21	10632.0
22	13129.0
23	15690.0
24	17757.0
25	20181.0
26	22372.0
27	24470.0
28	27451.0
29	31836.0
30	38496.0
31	47591.0
32	59113.0
33	74798.0
34	96212.0
35	126053.0
36	170511.0
37	265058.0
38	688251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12277658069419	18.291431411050638	12.200420055572314	24.385371952682853
2	15.186596729493004	20.067925993344666	41.11320367195771	23.63227360520462
3	19.57866777025096	25.65311054042509	28.67094578744598	26.09727590187797
4	12.289367674061857	16.096230527121815	37.646326691064054	33.96807510775227
5	13.012864378027093	38.83653081796054	34.66093482376274	13.489669980249625
6	31.855566216842373	36.083689051268486	18.31921737953771	13.74152735235143
7	28.540272403755818	31.822587482015642	21.51951741695673	18.11762269727181
8	26.244233004313166	35.843169503403935	20.40953808152516	17.503059410757743
9	27.987550495381413	14.488182760860127	19.723504949713494	37.800761794044966
10	16.391462766993577	28.561418005778155	33.02882515650022	22.01829407072805
11	34.7797849775433	22.627540794703478	24.019486730508397	18.573187497244813
12	24.452635383317997	24.79451879230929	30.59315669271761	20.159689131655103
13	29.660393606142694	21.989456337602224	25.457537027117766	22.89261302913731
14	21.764878355838007	22.48517828602243	27.060712898043626	28.68923046009594
15	23.756850004946408	30.768907448879013	23.26574610528107	22.208496440893512
16	22.323550337593318	27.928491317710886	27.472181613517677	22.27577673117812
17	21.917499220762178	28.480168397394884	27.251330856177304	22.351001525665634
18	21.758056635994006	26.69900548396209	29.93652712477257	21.606410755271334
19	23.653266486220474	26.49155183727034	27.80896284448819	22.046218832020998
20	24.414401113834867	26.361048997866448	28.164981663349977	21.05956822494871
21	23.68781733954011	26.21556484772315	27.190712798554166	22.905905014182572
22	23.945259190371655	27.591519214344085	26.983698131934496	21.47952346334976
23	22.64050254471188	27.283012561567265	28.030226912019817	22.04625798170104
24	23.550766612500578	26.32876937035765	28.35066819083551	21.769795826306265
25	22.897476038305904	26.818489846844045	28.202419287869425	22.081614826980626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	421.0
1	421.0
2	838.0
3	1255.0
4	1255.0
5	1255.0
6	2371.5
7	3488.0
8	3488.0
9	3488.0
10	3983.0
11	4478.0
12	4478.0
13	4478.0
14	6057.5
15	7637.0
16	7637.0
17	7637.0
18	14107.5
19	20578.0
20	20578.0
21	20578.0
22	32391.0
23	44204.0
24	44204.0
25	44204.0
26	66018.0
27	87832.0
28	87832.0
29	87832.0
30	114172.0
31	140512.0
32	140512.0
33	140512.0
34	157727.0
35	174942.0
36	174942.0
37	174942.0
38	187449.5
39	199957.0
40	199957.0
41	199957.0
42	213580.5
43	227204.0
44	227204.0
45	227204.0
46	245716.5
47	264229.0
48	264229.0
49	264229.0
50	256113.5
51	247998.0
52	247998.0
53	247998.0
54	220100.5
55	192203.0
56	192203.0
57	192203.0
58	167164.5
59	142126.0
60	142126.0
61	142126.0
62	119599.5
63	97073.0
64	97073.0
65	97073.0
66	75155.5
67	53238.0
68	53238.0
69	53238.0
70	39745.5
71	26253.0
72	26253.0
73	26253.0
74	18548.5
75	10844.0
76	10844.0
77	10844.0
78	7444.5
79	4045.0
80	4045.0
81	4045.0
82	2743.0
83	1441.0
84	1441.0
85	1441.0
86	967.0
87	493.0
88	493.0
89	493.0
90	301.0
91	109.0
92	109.0
93	109.0
94	72.5
95	36.0
96	36.0
97	36.0
98	25.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34200360440456395
2	0.009832987727714328
3	0.009474493383474742
4	0.011062111193678618
5	0.012444875092888446
6	0.08332432829682923
7	0.08245369917510452
8	0.10611432589491712
9	0.08655077739498548
10	0.0929524621135495
11	0.08936751867115364
12	0.09966142769860459
13	0.08583378870650632
14	0.0899820804041358
15	0.08706291217247061
16	0.08926509171565662
17	0.10176118028629359
18	0.10314394418550342
19	0.09684468642243642
20	0.0959228438229632
21	0.08322190134133219
22	0.10432185417371918
23	0.09725439424442452
24	0.09325974298004057
25	0.09162091169208818
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1952611.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.00477039370673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.21771584394403	41.37333189535218
2	13.772609766895355	15.151184759004032
3	4.904959069667626	8.093884422527914
4	2.2076183259296966	4.857181565388087
5	1.19296449079694	3.2809368952065467
6	0.6916316262568966	2.282582327957195
7	0.43695165139711245	1.6824097680774408
8	0.29814807796963627	1.3119653257635853
9	0.22422146466745402	1.109992516323654
>10	0.9450885998644928	8.946651677136796
>50	0.059769068686671964	2.2744901795684203
>100	0.04033265326122009	4.233116017009896
>500	0.005357571267948619	2.026239996608512
>1k	0.0023498119596265864	2.3700990039937744
>5k	2.8197743515519035E-4	1.0059336500819507
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9145	0.4683472540101433	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5164	0.2644663990933166	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5148	0.2636469834493404	No Hit
GTCCTACAGTGGACATTTCTAAATT	3746	0.1918456876459264	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3486	0.17853018343131324	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3267	0.1673144318043891	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2799	0.1433465242180854	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2708	0.13868609774297083	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2525	0.1293140313149931	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2502	0.12813612132677732	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2257	0.11558881927839185	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2012	0.10304151723000637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.0242695549702425E-4	0.0
10	0.0	0.0	0.0	1.0242695549702425E-4	0.0
11	0.0	0.0	0.0	1.0242695549702425E-4	0.0
12	0.0	0.0	0.0	1.0242695549702425E-4	0.0
13	0.0	0.0	0.0	1.0242695549702425E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	40	0.0052849916	14.247866	15
TCTATAC	165	0.0	12.089409	3
GTCCTAG	330	0.0	11.829765	1
CCGTACA	225	0.0	11.821058	17
TAGGACC	695	0.0	11.617262	4
CCGAATG	190	0.0	11.498868	18
CGAATGG	200	0.0	11.398584	19
GTCCTAC	1915	0.0	11.336326	1
GGTATCA	2325	0.0	11.22106	1
TAAGACG	60	0.005881999	11.081959	4
TCCGAAT	210	0.0	10.856074	17
CCCGTAC	245	0.0	10.856073	16
GTCCTAA	980	0.0	10.5902605	1
TCCTACA	2195	0.0	10.559054	2
TCGAACT	180	3.45608E-11	10.554245	19
AATGTCC	570	0.0	10.498696	8
GACGTGG	1015	0.0	10.481458	7
TGTAGGA	2165	0.0	10.4421215	2
TTCCGAA	220	0.0	10.362616	16
TGTCCCG	360	0.0	10.290389	11
>>END_MODULE