FastQCFastQC Report
Thu 26 May 2016
SRR1512848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952611
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT112280.5750249281602942No Hit
TATCAACGCAGAGTACTTTTTTTTT81400.41687770887288866No Hit
GGTATCAACGCAGAGTACTTTTTTT65120.33350216709831093No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41260.21130680919036102No Hit
GTCCTACAGTGGACATTTCTAAATT36260.18570007031610494No Hit
CTGTAGGACGTGGAATATGGCAAGA32140.16460011748371794No Hit
GTCCTAAAGTGTGTATTTCTCATTT32050.16413919618398135No Hit
CTTTAGGACGTGAAATATGGCGAGG29030.14867272590393069No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25960.13295018823513746No Hit
GTACTGGTTCACTATCGGTCAGTCA24770.12685578438306452No Hit
GTACATGGGAAGCAGTGGTATCAAC24380.12485845875087255No Hit
GTCCTACAGTGTGCATTTCTCATTT22300.11420605537918202No Hit
CCCATGTACTCTGCGTTGATACCAC22110.1132329993019603No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21460.10990412324830702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAG655.4014447E-513.16739753
TAGGACC6750.012.9601564
CTACCGC604.060976E-412.6784144
AAGGCGT1750.012.4969736
TCCCGAC550.003045353312.1033652
TAAGGCC550.003047694712.1021224
CGTGCGC1353.7471182E-1011.95841410
GGTATCA21850.011.5803991
GTGCGCC1657.2759576E-1211.50841511
GCGTGCG1354.7493813E-911.256429
GGCGAGG8400.011.18815919
TGGCGAG17450.011.09809918
GCAACCG600.005887612711.0805399
GATATAC3450.011.0289521
GACGTGG8600.010.9416987
GCGCCAC1658.185452E-1110.93243413
TGTAGGA18800.010.824972
TAGAAAT4800.010.69741254
GTCCTAC19400.010.6892711
GTAGGAC18800.010.6732183
Produced by FastQC (version 0.11.5)