##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512848_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1952611
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.040444819782333	31.0	30.0	34.0	26.0	34.0
2	30.052614166364933	31.0	30.0	34.0	26.0	34.0
3	30.218470038323044	31.0	30.0	34.0	26.0	34.0
4	33.79156063342878	37.0	35.0	37.0	30.0	37.0
5	33.35265908058492	37.0	35.0	37.0	27.0	37.0
6	33.19162086047861	37.0	35.0	37.0	26.0	37.0
7	33.01638472793608	37.0	35.0	37.0	26.0	37.0
8	32.94829845780855	37.0	35.0	37.0	26.0	37.0
9	34.23860666563898	39.0	35.0	39.0	25.0	39.0
10	34.16338226098286	39.0	35.0	39.0	25.0	39.0
11	34.12085202838661	39.0	35.0	39.0	25.0	39.0
12	33.9893977858365	39.0	35.0	39.0	23.0	39.0
13	33.968598968253275	39.0	35.0	39.0	23.0	39.0
14	35.030775203048634	39.0	35.0	41.0	23.0	41.0
15	35.02193831746313	39.0	35.0	41.0	24.0	41.0
16	35.02726195847509	39.0	35.0	41.0	24.0	41.0
17	35.02187942196372	40.0	35.0	41.0	24.0	41.0
18	34.95188749832916	40.0	35.0	41.0	23.0	41.0
19	34.965644974856744	40.0	35.0	41.0	22.0	41.0
20	34.94325905159809	40.0	35.0	41.0	22.0	41.0
21	34.90776350230537	40.0	35.0	41.0	22.0	41.0
22	34.86841260240775	40.0	35.0	41.0	22.0	41.0
23	34.707805087649305	40.0	35.0	41.0	19.0	41.0
24	34.65407395533468	40.0	35.0	41.0	18.0	41.0
25	34.5915182286692	39.0	35.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6638509934076602
1101	2	0.6495245526144586
1101	3	0.5090002986088535
1101	4	0.6374232598197196
1101	5	0.6908827738417713
1101	6	0.8470735067876518
1101	7	0.9235652279616389
1101	8	0.7091310739052687
1101	9	0.9140223266216978
1101	10	0.7193636050970866
1101	11	0.9785825936552612
1101	12	1.078726289487669
1101	13	1.202076437676439
1101	14	1.1873915485090478
1101	15	0.8791863239779545
1101	16	1.122196317954078
1101	17	1.2180141649342104
1101	18	1.1060110839561403
1101	19	0.9914771071902919
1101	20	1.0102851324426894
1101	21	1.0952422040694216
1101	22	1.0914996212288486
1101	23	1.2524170223852948
1101	24	1.3422221075065153
1101	25	1.4471295591014126
1102	1	0.8212602654606691
1102	2	0.8983755123285242
1102	3	0.6183935003088621
1102	4	0.58606224357721
1102	5	0.836213252212417
1102	6	0.7775143866385648
1102	7	0.8066124485513413
1102	8	0.8242280362377485
1102	9	0.9742535000561148
1102	10	0.9894993802180849
1102	11	1.0186536754678457
1102	12	1.0281993406399934
1102	13	1.0946605213964418
1102	14	1.0754936330610576
1102	15	1.1311865559550895
1102	16	1.1079237877456833
1102	17	1.125733386751385
1102	18	0.5151997987251846
1102	19	0.9016421351759547
1102	20	1.07491314021118
1102	21	0.9986595743163491
1102	22	0.8348614448248526
1102	23	1.033121684897381
1102	24	1.0158218309605473
1102	25	1.0707909528547432
1103	1	0.6687296830611125
1103	2	0.8058358391995348
1103	3	0.6046559588900493
1103	4	0.360391472907331
1103	5	0.45080584919666933
1103	6	0.4674961393048491
1103	7	0.6202327327354453
1103	8	0.6616416578394322
1103	9	0.6884317442278345
1103	10	0.6292476065377244
1103	11	0.7413772053058594
1103	12	0.865487141276347
1103	13	0.9133854477812946
1103	14	0.9635920237384497
1103	15	0.9739182542746221
1103	16	1.0062802102142712
1103	17	0.8698510919350753
1103	18	0.9102242712323161
1103	19	0.8796063387819473
1103	20	0.8566315515732796
1103	21	1.1087377677644312
1103	22	0.8896890832773181
1103	23	0.983054821923659
1103	24	0.9595041033794018
1103	25	0.9127782653584475
1104	1	0.9595170412959462
1104	2	1.1112536453176531
1104	3	0.9668128996994909
1104	4	0.9040430694447039
1104	5	1.0239472107949226
1104	6	0.9228403025939542
1104	7	0.9207020018113354
1104	8	1.0000434017712436
1104	9	1.0877296898430941
1104	10	1.1467208634217485
1104	11	1.2396471971039844
1104	12	1.190079930153125
1104	13	1.1334517589608666
1104	14	1.323272520463135
1104	15	1.375766830694559
1104	16	1.30096596092514
1104	17	1.2975492803432402
1104	18	0.8524591407311277
1104	19	1.3360313220844944
1104	20	1.3335512145349568
1104	21	1.3582925386667029
1104	22	1.239437044230307
1104	23	1.28741095298831
1104	24	1.6100594159970214
1104	25	1.5262156341447621
1105	1	0.7873300619760606
1105	2	0.7529152228397393
1105	3	0.7400308322922022
1105	4	0.6134801409222419
1105	5	0.5855954172144564
1105	6	0.827005594606625
1105	7	0.8235273119513593
1105	8	0.7870088315364754
1105	9	1.0269625382559298
1105	10	0.7491214719494366
1105	11	0.9533673394596391
1105	12	1.0707309279977082
1105	13	1.1504782180457411
1105	14	1.1890416258138572
1105	15	1.1712716004514476
1105	16	1.0422017307303122
1105	17	0.919536873678787
1105	18	0.870461524386478
1105	19	1.1379755348496658
1105	20	1.138595391713345
1105	21	1.0056636365371787
1105	22	1.0144338907687214
1105	23	1.196167004390695
1105	24	1.1404618700176385
1105	25	1.0832896727431347
1106	1	0.1283128270042475
1106	2	0.3208638689486172
1106	3	-1.4561241310949953
1106	4	-0.2668985015991012
1106	5	-0.030719548852118805
1106	6	0.290821106532114
1106	7	0.505215242962521
1106	8	0.49688272685651214
1106	9	0.6864956823581707
1106	10	0.4848420282553434
1106	11	0.6108027107306526
1106	12	0.7973277504869714
1106	13	0.7177476804117475
1106	14	0.6742960282706392
1106	15	0.7030428433802385
1106	16	0.7177042268674398
1106	17	0.7990534133648239
1106	18	0.7676948087052224
1106	19	0.813485115881214
1106	20	0.9654653690867647
1106	21	0.8206821957401687
1106	22	0.8538235206731599
1106	23	0.8255368545580595
1106	24	0.7603328090044599
1106	25	0.8202919508587314
1107	1	0.7774432889209031
1107	2	0.8253371693007487
1107	3	0.7962440525165846
1107	4	0.5778380983329896
1107	5	0.6508656643991699
1107	6	0.6221966173017748
1107	7	0.6958076670174265
1107	8	0.5055806463328736
1107	9	0.6974974202707926
1107	10	0.691615973740106
1107	11	0.6662089094619645
1107	12	0.90441162195971
1107	13	0.7189765951313234
1107	14	0.8131247830557129
1107	15	0.8139807978898759
1107	16	0.9036415202721457
1107	17	0.9745549804479339
1107	18	0.9548322455432583
1107	19	0.8852693693139315
1107	20	0.8951084676273595
1107	21	0.8229790329084352
1107	22	0.8536446521251406
1107	23	0.9766078974219852
1107	24	0.9440235092150573
1107	25	1.084275271974846
1108	1	0.45494603897141417
1108	2	0.4244843985355722
1108	3	0.41687014564890745
1108	4	0.34079496896939077
1108	5	0.5305825367498898
1108	6	0.33866273606722075
1108	7	0.3499859070259177
1108	8	0.4131770103477237
1108	9	0.6034202841601299
1108	10	0.5133291276551759
1108	11	0.4744163051080079
1108	12	0.6548042822330586
1108	13	0.5166093067525566
1108	14	0.5870896109811241
1108	15	0.7012881157793842
1108	16	0.6364619190141667
1108	17	0.6300181420162119
1108	18	0.665334275172377
1108	19	0.6630011031621024
1108	20	0.7901830710384488
1108	21	0.835055887381273
1108	22	0.8939924340231755
1108	23	0.860928629843599
1108	24	0.8829231012483802
1108	25	0.7708119634650998
1109	1	0.1373208210364396
1109	2	0.08644421023810978
1109	3	0.3251599056710397
1109	4	0.1945438200256362
1109	5	-0.14174640251075488
1109	6	0.20229599281115185
1109	7	0.0923709603094025
1109	8	0.15620165429318433
1109	9	0.40138239970271883
1109	10	0.008392518150728279
1109	11	0.24544356653104415
1109	12	0.5747139226049427
1109	13	0.36446277809187677
1109	14	0.5336472543974295
1109	15	0.3186761966502445
1109	16	0.38679084842232214
1109	17	0.4884728901558333
1109	18	0.5910555491154597
1109	19	0.6094560138677778
1109	20	0.46604176665938724
1109	21	0.6880845831623148
1109	22	0.6100380929144507
1109	23	0.6192397163964714
1109	24	0.5863930858497639
1109	25	0.45917982908373034
1110	1	0.3937204568988797
1110	2	0.24575296190221962
1110	3	0.29813965230731654
1110	4	0.3568686605621991
1110	5	0.5277298994291684
1110	6	0.5243187544737182
1110	7	0.47058227248175655
1110	8	0.5526232160591533
1110	9	0.5167861132110616
1110	10	0.7306362757560834
1110	11	0.5611148659728826
1110	12	0.5991494531944426
1110	13	0.6204236915417738
1110	14	0.6935491089038379
1110	15	0.6953925422785048
1110	16	0.6580099838306168
1110	17	0.7720579899411746
1110	18	0.6416986646780458
1110	19	0.7434513814331751
1110	20	0.778470668392309
1110	21	0.863922870939291
1110	22	0.8837411082881275
1110	23	0.9886179341454238
1110	24	0.833823739815891
1110	25	0.7371311159789684
1111	1	0.11607603931836152
1111	2	0.03296832987308562
1111	3	0.0321741002113356
1111	4	0.21599390119467188
1111	5	0.23941892354066852
1111	6	0.2765686363796789
1111	7	0.16194439197768418
1111	8	0.1319717433122065
1111	9	0.09749742027079833
1111	10	0.14217241253183488
1111	11	0.01692433076562594
1111	12	0.15941957108530858
1111	13	0.21055052199937307
1111	14	-0.03552386559293552
1111	15	0.07026060711086046
1111	16	0.03814072535958957
1111	17	0.19045323164030492
1111	18	0.040682801982050876
1111	19	-0.06735384213281037
1111	20	0.13358223551289683
1111	21	0.3116912745618521
1111	22	0.1617527602332487
1111	23	0.18869056832818387
1111	24	0.318586307307271
1111	25	0.3208412497172972
1112	1	0.13530118425961035
1112	2	-0.2457703144139174
1112	3	0.05753833815280274
1112	4	0.12655647260271508
1112	5	0.052175979844356846
1112	6	0.06692241547798261
1112	7	0.11910776345939666
1112	8	0.15874698899635575
1112	9	0.04430668881968103
1112	10	-0.12778991134518947
1112	11	0.0405600007337128
1112	12	0.20119898107903822
1112	13	0.1721611534868117
1112	14	-0.05118278283195821
1112	15	-0.2065908914840051
1112	16	-0.35681848667005767
1112	17	-0.20245428627938367
1112	18	0.14343134750964026
1112	19	0.10309939464386275
1112	20	0.03304122946022403
1112	21	0.03113652578154813
1112	22	0.25690384421872636
1112	23	0.36837055909889926
1112	24	0.29240509557919836
1112	25	0.13770867692283417
1113	1	-0.7588468089078013
1113	2	-0.6844848203993941
1113	3	-0.6219483741067755
1113	4	-1.9931244029645931
1113	5	-0.7177402752774711
1113	6	-0.4961882349222151
1113	7	-0.6193718645850126
1113	8	-0.496676097384146
1113	9	-0.4629732579642507
1113	10	-0.7576858889990277
1113	11	-0.7504737713646605
1113	12	-0.3779715918597546
1113	13	-0.4882055894182713
1113	14	-0.5361362218426251
1113	15	-0.5905335077237055
1113	16	-0.7655074349381437
1113	17	-0.8613914308376209
1113	18	-0.724504988120124
1113	19	-0.4932885994735372
1113	20	-0.3870990421358229
1113	21	-0.5040167498873913
1113	22	-0.4868234795260804
1113	23	-0.1240613223057565
1113	24	-0.1205054929485172
1113	25	-0.3310823670862746
1114	1	-0.9677943403361695
1114	2	-1.9814698559568278
1114	3	-1.3969435602524207
1114	4	-1.0442907659742318
1114	5	-0.9653162351871529
1114	6	-0.6234332403110159
1114	7	-0.7981099784328052
1114	8	-0.705239279832746
1114	9	-1.0190343480812274
1114	10	-1.1918233032513967
1114	11	-1.1632454829326093
1114	12	-1.1949634400381086
1114	13	-1.2879869997501316
1114	14	-1.1778574500453516
1114	15	-1.38236823440797
1114	16	-1.611340101563954
1114	17	-1.8628099313341977
1114	18	-1.4483034365131644
1114	19	-1.9166475361839659
1114	20	-1.3588260144194066
1114	21	-1.587526681614733
1114	22	-1.22007443944932
1114	23	-1.0914875564288522
1114	24	-1.1561799776762598
1114	25	-1.7847751493422166
1115	1	-1.1243222537115116
1115	2	-1.2725871675748266
1115	3	-0.7460149634653419
1115	4	-0.4581620271795188
1115	5	-0.7846292732629507
1115	6	-0.8111928480149331
1115	7	-0.6014582521525114
1115	8	-0.9474776751464979
1115	9	-1.9812475592288266
1115	10	-1.655444941664463
1115	11	-1.8849787526583555
1115	12	-2.5479877588452453
1115	13	-2.667633311989416
1115	14	-2.184211736900771
1115	15	-2.3966338996249803
1115	16	-2.0341173492785174
1115	17	-1.920744053105743
1115	18	-2.3546272139162028
1115	19	-2.877306984129291
1115	20	-2.955725144622658
1115	21	-2.3444290291784142
1115	22	-2.411679902305444
1115	23	-2.453609239219304
1115	24	-2.92883074984276
1115	25	-2.898924878640166
1116	1	-1.877826520615013
1116	2	-2.032924030918071
1116	3	-2.0166191840314305
1116	4	-1.7072386576772587
1116	5	-2.1582285318643315
1116	6	-3.0505077300476025
1116	7	-2.5824106790745525
1116	8	-2.9597918428107057
1116	9	-3.9160134104857285
1116	10	-2.8370229236701014
1116	11	-3.7645244046643818
1116	12	-5.933450360691019
1116	13	-4.894307251055718
1116	14	-4.118421056513082
1116	15	-5.309226333111482
1116	16	-4.744203700979487
1116	17	-4.588827952785241
1116	18	-4.314385100930139
1116	19	-4.706710003784078
1116	20	-6.430985309892765
1116	21	-5.375245098607309
1116	22	-4.567861844229224
1116	23	-5.205372957024451
1116	24	-5.117402061191566
1116	25	-4.444830775076046
1201	1	0.7140298745264424
1201	2	0.8516129893903965
1201	3	0.8490127904412503
1201	4	0.7859388427059599
1201	5	0.3196482482649614
1201	6	0.8183865465728388
1201	7	1.0688257759784605
1201	8	1.0517103148217615
1201	9	0.8435776086976503
1201	10	1.1602870950250619
1201	11	0.8575231984456622
1201	12	1.2500571910837266
1201	13	1.3189373925125878
1201	14	1.290495707109116
1201	15	1.0637175840909734
1201	16	1.0620765729228552
1201	17	1.0026024410453331
1201	18	1.2513208034113603
1201	19	1.432504657604163
1201	20	1.4057607197383462
1201	21	1.1810198343582954
1201	22	1.0552235640829508
1201	23	1.0582732034752382
1201	24	0.9905137888197899
1201	25	1.2447592441960822
1202	1	0.9776503480792442
1202	2	0.9533957778192601
1202	3	0.9036899689050699
1202	4	0.8413844389364087
1202	5	0.9111709380253927
1202	6	0.9302822303522547
1202	7	1.1202832313687239
1202	8	0.9788017450228779
1202	9	1.2695628484537522
1202	10	1.1568162765340446
1202	11	1.1700346909952017
1202	12	1.2941915418249934
1202	13	1.2521589500081234
1202	14	1.3553685656572014
1202	15	1.3057607376345075
1202	16	1.4196307456626869
1202	17	1.3425169962352825
1202	18	1.3161691006609644
1202	19	1.4721647950224366
1202	20	1.3265861679628514
1202	21	1.550769897927104
1202	22	1.453872846174832
1202	23	1.6221423930697156
1202	24	1.5281174109095659
1202	25	1.6044137369016482
1203	1	0.8311208191325221
1203	2	0.9312214984720377
1203	3	0.6619974928471741
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2211	11	-0.07927283438165489
2211	12	-0.35899025290903097
2211	13	-0.24478230667671852
2211	14	-0.25859217131201717
2211	15	-0.38354380157068846
2211	16	-0.2731822248315652
2211	17	-0.4391028609090881
2211	18	-0.4615892392326586
2211	19	-0.34560760422871795
2211	20	-0.4671523555418773
2211	21	-0.37814896375194706
2211	22	-0.3289168084705665
2211	23	-0.4927451144477786
2211	24	-0.2856252586317751
2211	25	-0.4550716355031099
2212	1	0.11335513951506115
2212	2	0.2932315200949738
2212	3	0.2659087722124198
2212	4	0.25443331376983735
2212	5	0.01132759550403506
2212	6	0.3080510476331355
2212	7	0.10440352878266879
2212	8	0.36990306429428443
2212	9	0.219517594076045
2212	10	-0.09644703410300082
2212	11	0.08722946606896897
2212	12	0.4227564911674122
2212	13	0.5155516663268429
2212	14	0.5394244867539086
2212	15	0.2360666749518927
2212	16	0.26652504200748695
2212	17	0.21135726563677082
2212	18	0.09825832632105858
2212	19	0.403701604993401
2212	20	0.3073135739274875
2212	21	0.2720334208308657
2212	22	0.35113849849778234
2212	23	0.06626006774480686
2212	24	0.0376420358004097
2212	25	0.16903339071067336
2213	1	-0.39551446451017824
2213	2	-0.02020453327238414
2213	3	-0.09063690804217828
2213	4	-0.3067160205069541
2213	5	-0.0391298520060559
2213	6	-0.07892366438314724
2213	7	-0.47706082585715137
2213	8	-0.44265368113818937
2213	9	-0.3845680978003543
2213	10	-0.25279804304663855
2213	11	-0.1934084013272468
2213	12	-0.40145170655410567
2213	13	-0.28662800117032816
2213	14	-0.34779390139998867
2213	15	-0.674453995628248
2213	16	-1.1721846026318232
2213	17	-0.7097534661921472
2213	18	-0.5226737857143888
2213	19	-0.4340101910469727
2213	20	-0.42697097426792396
2213	21	-0.4125066658639227
2213	22	-0.5266765830435176
2213	23	-0.6295079932194341
2213	24	-0.47721495015596105
2213	25	-0.4783539655878073
2214	1	-1.2244013328785464
2214	2	-0.6718199898224313
2214	3	-0.8210072752129918
2214	4	-0.8059159591714362
2214	5	-1.2223061030670053
2214	6	-1.7035386436392201
2214	7	-1.185846706594127
2214	8	-1.0228225481805033
2214	9	-0.8281332678660291
2214	10	-1.1229631738057506
2214	11	-1.0928707436960536
2214	12	-0.8383950165554594
2214	13	-1.227168941547454
2214	14	-0.9320233307853201
2214	15	-0.9919665812874001
2214	16	-1.1879334682487297
2214	17	-1.2485354378698261
2214	18	-1.2041263791916563
2214	19	-1.192160687670281
2214	20	-1.4377005861480043
2214	21	-1.536439287503697
2214	22	-1.8137947208626954
2214	23	-1.8821853748840027
2214	24	-1.420668571651035
2214	25	-2.0324566421055934
2215	1	-1.5241628165938579
2215	2	-1.0683439956426923
2215	3	-0.9875823429776744
2215	4	-1.199694746448884
2215	5	-1.5457058204095446
2215	6	-1.4611670694592256
2215	7	-2.0261809702087668
2215	8	-1.642278638701253
2215	9	-1.5872276425867113
2215	10	-1.6069149692193605
2215	11	-1.1742255205907028
2215	12	-1.4939381427074352
2215	13	-1.6647534790517895
2215	14	-1.8581157150301593
2215	15	-1.6798963599823225
2215	16	-1.8185028015080391
2215	17	-1.7366311170365591
2215	18	-1.5022564532971714
2215	19	-1.753456404436733
2215	20	-1.8253885503381326
2215	21	-1.5694772285787977
2215	22	-1.674029455188311
2215	23	-2.120837546239734
2215	24	-1.94200283361657
2215	25	-2.1939732296791092
2216	1	-1.9296562653024516
2216	2	-1.4186035774725418
2216	3	-1.4171159663176596
2216	4	-1.7819436615381115
2216	5	-2.6342086929893433
2216	6	-2.8274113044944116
2216	7	-2.702511519597966
2216	8	-3.0000384003976635
2216	9	-4.770534911844056
2216	10	-3.0513567742317314
2216	11	-3.0818799253778693
2216	12	-3.531158643426391
2216	13	-3.2953302943486626
2216	14	-3.4630556634672764
2216	15	-3.494820203968853
2216	16	-2.8561795324807377
2216	17	-3.139868305962601
2216	18	-3.4089420223387066
2216	19	-3.6013672642739074
2216	20	-2.894281137210271
2216	21	-3.6240755101115063
2216	22	-3.0394614776192945
2216	23	-4.087742098983487
2216	24	-3.4386129882745315
2216	25	-3.6581980832256704
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63284.0
3	1.0
4	1069.0
5	8904.0
6	11486.0
7	8907.0
8	7268.0
9	5973.0
10	4995.0
11	4546.0
12	3912.0
13	3593.0
14	3407.0
15	3358.0
16	3564.0
17	3689.0
18	4202.0
19	4970.0
20	6787.0
21	8798.0
22	11084.0
23	13105.0
24	15274.0
25	17113.0
26	19137.0
27	20755.0
28	23200.0
29	27458.0
30	33943.0
31	43367.0
32	55125.0
33	70291.0
34	91714.0
35	120247.0
36	165269.0
37	255822.0
38	806994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.072319163648054	19.169798902995414	13.314289609825783	26.44359232353075
2	13.646857618323487	20.534663955961978	40.00711150095708	25.81136692475745
3	17.51704574267498	25.583750416827378	29.444583600333356	27.454620240164285
4	11.513645126330864	16.73148980892111	36.8627886823608	34.89207638238723
5	12.577501352303836	39.05540552723458	34.73540946120937	13.631683659252216
6	31.943963317269947	36.62431153263546	17.86264064002099	13.569084510073607
7	28.007841629828945	32.03172528669357	21.504004100198603	18.456428983278876
8	26.079980814689062	36.22610995786303	20.119057562594385	17.574851664853526
9	27.192187115810178	14.404147201049094	19.721385542168672	38.682280140972054
10	15.736181843056297	29.158897468099532	33.01887614219388	22.086044546650292
11	34.48436400438006	22.576853461389145	24.011028014598853	18.927754519631932
12	23.836567047610757	25.095551839721807	30.73709224343889	20.330788869228545
13	29.3103616054806	21.896842334929758	25.673512806999483	23.119283252590158
14	21.336196521359703	22.378856520683684	27.262237772981894	29.022709184974715
15	23.615140488688375	30.775207854417907	23.21085391389152	22.3987977430022
16	22.16785655933133	27.74694135525351	27.478299973827312	22.606902111587843
17	21.513030090872356	28.528224309212867	27.535865207969806	22.422880391944968
18	21.51154601993044	26.61779234833861	30.23173986461397	21.63892176711698
19	23.35430727109028	26.333849231427926	28.26467723250547	22.047166264976322
20	23.997652350133745	26.211275172322313	28.562663660746157	21.228408816797785
21	23.314790592674612	26.032594389241865	27.623186654143232	23.029428363940294
22	23.4638702613231	27.414444930405686	27.50524684497916	21.61643796329206
23	22.182861819379283	27.01311218670795	28.465936123477743	22.338089870435024
24	23.23109928193583	26.18872883539015	28.75585562101207	21.82431626166195
25	22.491883944113805	26.707418938027082	28.542551486189517	22.258145631669596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	198.0
1	198.0
2	525.0
3	852.0
4	852.0
5	852.0
6	2049.0
7	3246.0
8	3246.0
9	3246.0
10	3860.5
11	4475.0
12	4475.0
13	4475.0
14	5941.5
15	7408.0
16	7408.0
17	7408.0
18	14287.5
19	21167.0
20	21167.0
21	21167.0
22	33821.0
23	46475.0
24	46475.0
25	46475.0
26	69569.0
27	92663.0
28	92663.0
29	92663.0
30	117452.0
31	142241.0
32	142241.0
33	142241.0
34	159493.5
35	176746.0
36	176746.0
37	176746.0
38	188810.0
39	200874.0
40	200874.0
41	200874.0
42	215120.5
43	229367.0
44	229367.0
45	229367.0
46	246294.0
47	263221.0
48	263221.0
49	263221.0
50	254517.5
51	245814.0
52	245814.0
53	245814.0
54	218404.0
55	190994.0
56	190994.0
57	190994.0
58	165861.5
59	140729.0
60	140729.0
61	140729.0
62	117723.5
63	94718.0
64	94718.0
65	94718.0
66	72864.5
67	51011.0
68	51011.0
69	51011.0
70	37936.0
71	24861.0
72	24861.0
73	24861.0
74	17479.5
75	10098.0
76	10098.0
77	10098.0
78	6917.0
79	3736.0
80	3736.0
81	3736.0
82	2505.0
83	1274.0
84	1274.0
85	1274.0
86	818.5
87	363.0
88	363.0
89	363.0
90	217.5
91	72.0
92	72.0
93	72.0
94	39.5
95	7.0
96	7.0
97	7.0
98	4.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08511680001802714
2	0.04317296174199572
3	0.01864170590045841
4	0.0060944038520729426
5	0.019819615888674192
6	0.060175836354501744
7	0.07610322793428902
8	0.05725666812283656
9	0.023097278464578968
10	0.05090619688202105
11	0.006606538629558063
12	3.0728086649107274E-4
13	0.006350471240815503
14	4.6092129973660906E-4
15	0.01449341420282893
16	0.00993541468321135
17	0.004711639952863116
18	0.006145617329821454
19	0.0014851908547068515
20	0.0010242695549702422
21	2.5606738874256056E-4
22	4.6092129973660906E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1952611.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.330125374709894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82108273720615	39.36887597672036
2	14.53560096130342	15.503708433261298
3	5.133377509715382	8.212909985665123
4	2.347469305920985	5.007633295921973
5	1.2577074337463292	3.353684756319818
6	0.7533650243268424	2.410623072016312
7	0.47480898615795175	1.772513593258972
8	0.31205864982638787	1.3313701535603166
9	0.24031509068179485	1.1534430523945407
>10	1.0115097551829897	9.321985639423353
>50	0.06269861491741868	2.3307428990040497
>100	0.04180179949868927	4.3225954274251555
>500	0.005308555686629865	1.9678436543065287
>1k	0.002606018246163751	2.609927926967257
>5k	1.930383886047223E-4	0.7541942250300617
>10k+	9.651919430236115E-5	0.5779479087249204
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11228	0.5750249281602942	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8140	0.41687770887288866	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6512	0.33350216709831093	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4126	0.21130680919036102	No Hit
GTCCTACAGTGGACATTTCTAAATT	3626	0.18570007031610494	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3214	0.16460011748371794	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3205	0.16413919618398135	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2903	0.14867272590393069	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2596	0.13295018823513746	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2477	0.12685578438306452	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2438	0.12485845875087255	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2230	0.11420605537918202	No Hit
CCCATGTACTCTGCGTTGATACCAC	2211	0.1132329993019603	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2146	0.10990412324830702	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.121347774851212E-5	0.0
5	0.0	0.0	0.0	5.121347774851212E-5	0.0
6	0.0	0.0	0.0	5.121347774851212E-5	0.0
7	0.0	0.0	0.0	5.121347774851212E-5	0.0
8	0.0	0.0	0.0	5.121347774851212E-5	0.0
9	0.0	0.0	0.0	1.5364043324553637E-4	0.0
10	0.0	0.0	0.0	1.5364043324553637E-4	0.0
11	0.0	0.0	0.0	1.5364043324553637E-4	0.0
12	0.0	0.0	0.0	1.5364043324553637E-4	0.0
13	0.0	0.0	0.0	1.5364043324553637E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTAG	65	5.4014447E-5	13.1673975	3
TAGGACC	675	0.0	12.960156	4
CTACCGC	60	4.060976E-4	12.678414	4
AAGGCGT	175	0.0	12.496973	6
TCCCGAC	55	0.0030453533	12.103365	2
TAAGGCC	55	0.0030476947	12.102122	4
CGTGCGC	135	3.7471182E-10	11.958414	10
GGTATCA	2185	0.0	11.580399	1
GTGCGCC	165	7.2759576E-12	11.508415	11
GCGTGCG	135	4.7493813E-9	11.25642	9
GGCGAGG	840	0.0	11.188159	19
TGGCGAG	1745	0.0	11.098099	18
GCAACCG	60	0.0058876127	11.080539	9
GATATAC	345	0.0	11.028952	1
GACGTGG	860	0.0	10.941698	7
GCGCCAC	165	8.185452E-11	10.932434	13
TGTAGGA	1880	0.0	10.82497	2
TAGAAAT	480	0.0	10.6974125	4
GTCCTAC	1940	0.0	10.689271	1
GTAGGAC	1880	0.0	10.673218	3
>>END_MODULE