FastQCFastQC Report
Thu 26 May 2016
SRR1512847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217873
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT59550.2685004957452478No Hit
GTATCAACGCAGAGTACTTTTTTTT53620.24176316678186713No Hit
GTCCTAAAGTGTGTATTTCTCATTT51430.2318888412456439No Hit
CTGTAGGACGTGGAATATGGCAAGA47360.21353792575138433No Hit
CTTTAGGACGTGAAATATGGCGAGG41050.18508724349861333No Hit
GTCCTACAGTGTGCATTTCTCATTT36690.16542876891508215No Hit
TATCAACGCAGAGTACTTTTTTTTT31580.142388675997228No Hit
GGTATCAACGCAGAGTACTTTTTTT29810.13440805672822564No Hit
CTGTAGGACCTGGAATATGGCGAGA28990.13071082068269915No Hit
GTACTGGTTCACTATCGGTCAGTCA27590.12439846645862951No Hit
CTGAAGGACCTGGAATATGGCGAGA22520.10153872651860589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGT400.005283621614.2485446
GGTATCA18400.012.627321
GCCGGTT1501.4551915E-1112.03156111
TAGGACC9850.011.86173254
CGTAGGA600.005881467411.0822012
ATAGGAC3100.011.0311323
GGCGAGG13700.010.88525819
GACACCG700.001493108710.8560335
TGGACCG700.001493108710.8560335
ATTATAC1855.456968E-1210.7826813
CTACACT2800.010.5167824
TGTAGGA28550.010.480542
GTATCAA37600.010.4339461
AGGACCT18950.010.4263755
GTCCTAA15350.010.3597031
CATTTCT37300.010.31349514
TAGAAAT7650.010.3061364
TCCCGCC4150.010.29968813
ATTTAGA7050.010.2651121
GGACGTG27150.010.2512556
Produced by FastQC (version 0.11.5)