##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512847_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2217873 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.721257258643753 31.0 30.0 34.0 18.0 34.0 2 28.755669508578716 31.0 30.0 34.0 16.0 34.0 3 28.93920120764354 31.0 30.0 34.0 20.0 34.0 4 32.323436012792435 35.0 33.0 37.0 22.0 37.0 5 32.53837753559379 35.0 33.0 37.0 25.0 37.0 6 32.40749312516993 36.0 33.0 37.0 23.0 37.0 7 32.36617966853828 36.0 33.0 37.0 22.0 37.0 8 32.28260139331693 36.0 33.0 37.0 22.0 37.0 9 33.581583345845324 38.0 34.0 39.0 19.0 39.0 10 33.43545279644055 38.0 33.0 39.0 18.0 39.0 11 33.51286345070254 38.0 34.0 39.0 18.0 39.0 12 33.405865439545 38.0 34.0 39.0 17.0 39.0 13 33.377812886490794 38.0 34.0 39.0 17.0 39.0 14 34.204088782360394 39.0 33.0 41.0 17.0 41.0 15 34.3907771094197 39.0 34.0 41.0 17.0 41.0 16 34.33678483844657 39.0 34.0 41.0 17.0 41.0 17 34.079780041508236 39.0 33.0 41.0 16.0 41.0 18 34.08342226989553 39.0 33.0 41.0 16.0 41.0 19 34.19704103886922 39.0 34.0 41.0 16.0 41.0 20 34.17218298793484 39.0 34.0 41.0 11.0 41.0 21 34.05593016371992 39.0 34.0 41.0 10.0 41.0 22 34.062435495630275 39.0 34.0 41.0 10.0 41.0 23 33.724073470392575 39.0 34.0 41.0 2.0 41.0 24 33.7931721067888 39.0 34.0 41.0 2.0 41.0 25 33.49842935100432 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6478768258591998 1101 2 0.29215703485786904 1101 3 0.6632842650085884 1101 4 0.6523947857206096 1101 5 0.5954261351607286 1101 6 0.6010524170922835 1101 7 0.25008476390731005 1101 8 0.6580582334542697 1101 9 0.633472794396674 1101 10 0.6943286244497671 1101 11 0.6580148174301925 1101 12 0.5820780291939798 1101 13 0.9936817762631591 1101 14 1.0229778408557735 1101 15 0.8056026154353333 1101 16 0.701595884557868 1101 17 0.02206371168499288 1101 18 0.4259857924209882 1101 19 0.018248349796913033 1101 20 -0.6670270340107294 1101 21 0.4323020887062796 1101 22 0.49266423778359325 1101 23 0.08373145071237076 1101 24 0.7283646547143263 1101 25 0.20706094357875315 1102 1 1.157485066187153 1102 2 1.1078440773713716 1102 3 1.1039449627971862 1102 4 1.3148560579454625 1102 5 1.1998975316301923 1102 6 1.0559249443144054 1102 7 1.0671826848360766 1102 8 1.087482053844333 1102 9 1.3760515894898475 1102 10 1.462472258223542 1102 11 1.206438824052178 1102 12 1.395042974122866 1102 13 1.3854117976272136 1102 14 1.4988306984176347 1102 15 1.508832938568311 1102 16 1.3408926508487014 1102 17 1.6785061694522128 1102 18 1.6116452936580359 1102 19 1.341605539424478 1102 20 1.578581026948882 1102 21 1.700899496925281 1102 22 1.5595507831905522 1102 23 1.4879070724501702 1102 24 0.9346036529604476 1102 25 1.4721996543322078 1103 1 0.5092675256954422 1103 2 0.6663333440602841 1103 3 0.8154205498931937 1103 4 0.8905055051381794 1103 5 0.710781602298411 1103 6 0.3158973892563637 1103 7 0.19432955754970038 1103 8 0.41547232936388667 1103 9 0.5284320354138217 1103 10 0.6800766521430006 1103 11 0.5913045474300631 1103 12 0.5650400449023181 1103 13 0.7065382136571827 1103 14 0.8037480221647542 1103 15 0.7282597419555685 1103 16 0.7207997932278758 1103 17 0.3984120621487932 1103 18 0.8250223924711477 1103 19 0.71890288367171 1103 20 0.8482915127164645 1103 21 0.6176725860298689 1103 22 0.5819032619271169 1103 23 0.08719342392206642 1103 24 0.329269989923624 1103 25 0.6183108592433726 1104 1 0.9097450835058289 1104 2 0.8271083563398172 1104 3 0.8463608917923651 1104 4 0.9728165877608816 1104 5 0.7304891483799452 1104 6 0.7340299010656821 1104 7 0.8305487166881349 1104 8 0.6295012055431926 1104 9 1.006123733194741 1104 10 1.056482839532805 1104 11 0.919177119339949 1104 12 1.1634422349834495 1104 13 1.041158310129184 1104 14 1.3493839629355406 1104 15 1.0861711994977838 1104 16 1.1398081843746368 1104 17 1.3505637169848228 1104 18 1.0968337279922622 1104 19 0.823269196106601 1104 20 0.9340627015060434 1104 21 1.089898824583976 1104 22 0.8758101935707714 1104 23 1.1503997582313232 1104 24 0.9901391322630673 1104 25 0.6878021584765648 1105 1 0.6437352806952816 1105 2 0.7245143610198888 1105 3 0.7748415041619943 1105 4 0.9987385393667694 1105 5 0.7191614314294625 1105 6 0.9109156151211693 1105 7 0.836685545599444 1105 8 0.8667017726105257 1105 9 0.5645373607781181 1105 10 0.6648824344455235 1105 11 0.6411589679047154 1105 12 0.8624693088655846 1105 13 0.794179448971704 1105 14 0.8594131649489114 1105 15 0.8287155824263053 1105 16 0.786263576340474 1105 17 1.1120882084955284 1105 18 0.9318202362266206 1105 19 0.5709988951525347 1105 20 0.868872159783237 1105 21 0.7874164219987421 1105 22 0.7807616089846263 1105 23 1.0655220739151545 1105 24 0.9479074798728035 1105 25 1.2863505099849348 1106 1 0.6265906988519383 1106 2 0.7195165496748892 1106 3 0.5383864545465968 1106 4 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1107 17 1.1176135145179842 1107 18 1.1133421617268056 1107 19 0.8690348358864242 1107 20 0.9472830228902112 1107 21 0.8417533169734668 1107 22 0.8064967786113897 1107 23 1.0771130628429333 1107 24 0.8862980575279948 1107 25 1.2123962038302594 1108 1 1.076915962650574 1108 2 0.8897198863067715 1108 3 0.9444110610165097 1108 4 1.099604473515221 1108 5 0.9276490266753115 1108 6 1.1868174958202218 1108 7 1.124755624151355 1108 8 1.0576303658535267 1108 9 1.2139581183057189 1108 10 1.2383356659715758 1108 11 0.9936616723208189 1108 12 1.060285283531691 1108 13 1.1781809825147747 1108 14 1.175734044281711 1108 15 1.143298076896528 1108 16 0.9702325932131686 1108 17 1.2026446545656952 1108 18 1.4188140229127981 1108 19 1.2494513662747622 1108 20 1.241537432665666 1108 21 1.2234892848112224 1108 22 1.216205474353373 1108 23 1.4696739366895102 1108 24 1.3405246079048752 1108 25 1.4916017372370192 1109 1 0.7944014041909782 1109 2 0.7477271289083731 1109 3 0.8533308147313257 1109 4 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-1.2061295277044266 2214 7 -1.11468258223233 2214 8 -0.9033160195481855 2214 9 -0.6562938175383835 2214 10 -0.6582400681765961 2214 11 -0.8102703921295955 2214 12 -0.6115688317476682 2214 13 -1.000926136237453 2214 14 -0.9661145487799274 2214 15 -1.3546245875121627 2214 16 -1.107453965708892 2214 17 -3.087503463585218 2214 18 -2.7866959232149 2214 19 -1.6929654016325628 2214 20 -1.5523807648824715 2214 21 -1.6002508518943444 2214 22 -2.578808576622837 2214 23 -1.356730608709256 2214 24 -2.5028248012178267 2214 25 -2.571737363745939 2215 1 -2.072129493119263 2215 2 -3.605300977696004 2215 3 -2.226344669118184 2215 4 -3.693845061129096 2215 5 -2.4793966000821435 2215 6 -2.396263630777373 2215 7 -2.43452262071931 2215 8 -1.8387925966123504 2215 9 -4.084307528525212 2215 10 -2.478297798647457 2215 11 -2.087098824386498 2215 12 -2.066020692953998 2215 13 -1.9696879379092316 2215 14 -1.6842395111949884 2215 15 -1.9400464409008222 2215 16 -2.0128000478545403 2215 17 -2.3861095132574768 2215 18 -1.942411841189518 2215 19 -2.1366360974050096 2215 20 -1.9884502819911347 2215 21 -2.5863738899603774 2215 22 -2.4979678766408746 2215 23 -2.4055922359869015 2215 24 -2.4021753334206046 2215 25 -3.4884948356324017 2216 1 -2.8720720661837795 2216 2 -2.799489597028611 2216 3 -4.164627228407742 2216 4 -4.645795231064032 2216 5 -4.452543689981571 2216 6 -3.379393248625515 2216 7 -4.99589981622049 2216 8 -2.913185250468679 2216 9 -3.4837077360879256 2216 10 -2.444136278822967 2216 11 -2.7550221275237696 2216 12 -3.1074994553097035 2216 13 -2.75587432021873 2216 14 -3.6426823028947304 2216 15 -3.0940275725867927 2216 16 -3.483423918317392 2216 17 -3.2248058842880276 2216 18 -2.773233590609152 2216 19 -3.5184073617922387 2216 20 -4.062395906565364 2216 21 -3.7373770784689846 2216 22 -3.4708916395689755 2216 23 -3.4401797660844338 2216 24 -3.800445393906557 2216 25 -3.8297422012186537 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 121763.0 3 5.0 4 1078.0 5 6564.0 6 7499.0 7 6728.0 8 5970.0 9 5545.0 10 5074.0 11 4800.0 12 4432.0 13 4375.0 14 4373.0 15 4452.0 16 4614.0 17 5074.0 18 5668.0 19 6819.0 20 8515.0 21 11194.0 22 13962.0 23 16736.0 24 19177.0 25 21881.0 26 24358.0 27 26856.0 28 30201.0 29 35901.0 30 42980.0 31 53586.0 32 67168.0 33 85202.0 34 110393.0 35 144329.0 36 194749.0 37 299070.0 38 806782.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.87059139670633 18.10372322017083 12.240695342502688 24.784990040620155 2 15.12779882631246 19.724020190960964 41.47827451517975 23.669906467546827 3 19.648918862370493 25.00162332977403 28.571370595365213 26.77808721249026 4 12.425685693774554 15.950384378122735 37.26037243732887 34.36355749077384 5 13.30251006836704 38.56611842861775 34.50720579873006 13.624165704285149 6 32.37257978414971 35.60582164937707 18.235725989170934 13.785872577302282 7 29.196042756424834 31.211847182963854 21.196721429834916 18.395388630776395 8 26.30001083267134 36.15079078500758 20.149536000577744 17.39966238174334 9 28.210190479198914 14.376238159919493 19.54756113520368 37.86601022567792 10 16.790059494824273 28.176077240826547 32.896084208892134 22.137779055457045 11 35.159756563146736 21.78480947537246 24.327258844351864 18.728175117128938 12 24.898869386139545 24.0227576038081 30.650778828712745 20.427594181339614 13 29.97773473741972 21.562377031129852 25.28136180013791 23.17852643131252 14 21.682309713370763 22.16680942319443 26.84731309349635 29.303567769938454 15 23.663841382130038 31.17161911277461 22.583533323525614 22.581006181569734 16 22.550639352141214 27.238499671693184 27.727236829932554 22.483624146233048 17 22.122597717241824 28.292381523641115 27.221455706286136 22.363565052830925 18 22.01629522778746 26.37995358405646 29.91914671211373 21.68460447604235 19 24.26754883797549 26.008633664974983 26.951521948599684 22.772295548449843 20 24.997653171197552 26.19047404136556 27.514491667855122 21.29738111958176 21 23.998054184991414 25.902895166960132 26.272248039067925 23.826802608980532 22 24.623179764747725 27.649251053562068 26.16024975864097 21.567319423049234 23 23.026069641502374 27.31460983996617 27.338439576498647 22.32088094203281 24 24.21429870526662 25.96317485647545 27.795676820756892 22.02684961750104 25 23.274865729999625 26.757965603593348 27.797150646404955 22.170018020002065 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 270.0 1 270.0 2 666.5 3 1063.0 4 1063.0 5 1063.0 6 1676.0 7 2289.0 8 2289.0 9 2289.0 10 2644.0 11 2999.0 12 2999.0 13 2999.0 14 4288.0 15 5577.0 16 5577.0 17 5577.0 18 11445.0 19 17313.0 20 17313.0 21 17313.0 22 30167.0 23 43021.0 24 43021.0 25 43021.0 26 67420.0 27 91819.0 28 91819.0 29 91819.0 30 122233.5 31 152648.0 32 152648.0 33 152648.0 34 174823.0 35 196998.0 36 196998.0 37 196998.0 38 212949.5 39 228901.0 40 228901.0 41 228901.0 42 246212.5 43 263524.0 44 263524.0 45 263524.0 46 281419.5 47 299315.0 48 299315.0 49 299315.0 50 292706.5 51 286098.0 52 286098.0 53 286098.0 54 256824.5 55 227551.0 56 227551.0 57 227551.0 58 198085.0 59 168619.0 60 168619.0 61 168619.0 62 141649.5 63 114680.0 64 114680.0 65 114680.0 66 89208.0 67 63736.0 68 63736.0 69 63736.0 70 47447.5 71 31159.0 72 31159.0 73 31159.0 74 22196.0 75 13233.0 76 13233.0 77 13233.0 78 9020.0 79 4807.0 80 4807.0 81 4807.0 82 3198.5 83 1590.0 84 1590.0 85 1590.0 86 1035.0 87 480.0 88 480.0 89 480.0 90 313.0 91 146.0 92 146.0 93 146.0 94 86.5 95 27.0 96 27.0 97 27.0 98 18.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33338247951979216 2 0.011136796381037147 3 0.009423443091646817 4 0.011948384781274671 5 0.012805061425969835 6 0.0811588400237525 7 0.08048251635688788 8 0.10609263920882755 9 0.08490116431373662 10 0.09243090113816256 11 0.08742610600336448 12 0.09802184345090995 13 0.0840895759134991 14 0.08922963578167009 15 0.08706540004770337 16 0.08828278264805965 17 0.1000959026959614 18 0.1016739912519788 19 0.0959477842058585 20 0.09545181351682445 21 0.0827369285797699 22 0.10343243278582677 23 0.09712007856175714 24 0.09243090113816256 25 0.09026666540419581 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2217873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.17053132496071 #Duplication Level Percentage of deduplicated Percentage of total 1 73.27287322504495 37.49411854632038 2 14.058093458934309 14.38720223419247 3 5.281284022166115 8.107383284767971 4 2.519431718326694 5.156826386549429 5 1.377651953311563 3.5247591215911327 6 0.8445835375682408 2.593067301940908 7 0.5597666485361339 2.005048977651056 8 0.3821474348359904 1.5643749828022742 9 0.27390031357118605 1.2614062117959843 >10 1.3027334058322269 11.379169271202013 >50 0.07467710519756594 2.671486020725272 >100 0.04442172230992293 4.474704489681961 >500 0.005150066875553354 1.9019261531338414 >1k 0.0030190047201518844 2.7306423457568365 >5k 2.663827694251663E-4 0.7478846718885409 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5955 0.2685004957452478 No Hit GTATCAACGCAGAGTACTTTTTTTT 5362 0.24176316678186713 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5143 0.2318888412456439 No Hit CTGTAGGACGTGGAATATGGCAAGA 4736 0.21353792575138433 No Hit CTTTAGGACGTGAAATATGGCGAGG 4105 0.18508724349861333 No Hit GTCCTACAGTGTGCATTTCTCATTT 3669 0.16542876891508215 No Hit TATCAACGCAGAGTACTTTTTTTTT 3158 0.142388675997228 No Hit GGTATCAACGCAGAGTACTTTTTTT 2981 0.13440805672822564 No Hit CTGTAGGACCTGGAATATGGCGAGA 2899 0.13071082068269915 No Hit GTACTGGTTCACTATCGGTCAGTCA 2759 0.12439846645862951 No Hit CTGAAGGACCTGGAATATGGCGAGA 2252 0.10153872651860589 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.5088244457640273E-5 0.0 0.0 0.0 0.0 8 4.5088244457640273E-5 0.0 0.0 0.0 0.0 9 4.5088244457640273E-5 0.0 0.0 4.5088244457640273E-5 0.0 10 9.017648891528055E-5 0.0 0.0 4.5088244457640273E-5 0.0 11 9.017648891528055E-5 0.0 0.0 4.5088244457640273E-5 0.0 12 9.017648891528055E-5 4.5088244457640273E-5 0.0 4.5088244457640273E-5 0.0 13 9.017648891528055E-5 4.5088244457640273E-5 0.0 4.5088244457640273E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATGCGT 40 0.0052836216 14.248544 6 GGTATCA 1840 0.0 12.62732 1 GCCGGTT 150 1.4551915E-11 12.031561 11 TAGGACC 985 0.0 11.8617325 4 CGTAGGA 60 0.0058814674 11.082201 2 ATAGGAC 310 0.0 11.031132 3 GGCGAGG 1370 0.0 10.885258 19 GACACCG 70 0.0014931087 10.856033 5 TGGACCG 70 0.0014931087 10.856033 5 ATTATAC 185 5.456968E-12 10.782681 3 CTACACT 280 0.0 10.516782 4 TGTAGGA 2855 0.0 10.48054 2 GTATCAA 3760 0.0 10.433946 1 AGGACCT 1895 0.0 10.426375 5 GTCCTAA 1535 0.0 10.359703 1 CATTTCT 3730 0.0 10.313495 14 TAGAAAT 765 0.0 10.306136 4 TCCCGCC 415 0.0 10.299688 13 ATTTAGA 705 0.0 10.265112 1 GGACGTG 2715 0.0 10.251255 6 >>END_MODULE