FastQCFastQC Report
Thu 26 May 2016
SRR1512847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217873
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66990.3020461496217322No Hit
GTCCTACAGTGGACATTTCTAAATT55420.24987905078424238No Hit
GTCCTAAAGTGTGTATTTCTCATTT49350.22251048639845475No Hit
TATCAACGCAGAGTACTTTTTTTTT47840.21570216148535104No Hit
CTGTAGGACGTGGAATATGGCAAGA46100.20785680694972164No Hit
CTTTAGGACGTGAAATATGGCGAGG44250.19951548172505823No Hit
GGTATCAACGCAGAGTACTTTTTTT39240.17692627125178043No Hit
GTCCTACAGTGTGCATTTCTCATTT35190.15866553224643612No Hit
CTGTAGGACCTGGAATATGGCGAGA29040.13093626190498736No Hit
GTACTGGTTCACTATCGGTCAGTCA24030.10834705143170957No Hit
CTGAAGGACCTGGAATATGGCGAGA23560.10622790394220047No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23360.10532613905304766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.013.1310111
TAGGACC12300.012.6024814
GACGTTG550.003054717812.0985237
CTACACT2850.012.012444
GGCGAGG12300.011.80772119
TGTAGGA28850.010.7476622
TAGGGTG1701.364242E-1010.6286545
TAGGACA8450.010.5790024
GTCCTAC30750.010.5787181
ACACCGT909.4188945E-510.5664976
TAGAAAT7500.010.5242314
GTATCAA36900.010.5168531
TGGCGAG28950.010.49255318
GACGTGA15500.010.4872467
GTAGGAC29400.010.4818983
CTGTAGG29450.010.2059581
GGACGTG27050.010.1602436
GTCCTAT2250.010.1456711
TTAGGAC18000.010.1454423
ATAGGAC3000.010.1454413
Produced by FastQC (version 0.11.5)