##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512847_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2217873
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.12207146216217	31.0	30.0	34.0	26.0	34.0
2	30.145389298665883	31.0	30.0	34.0	26.0	34.0
3	30.32387066346901	31.0	30.0	34.0	26.0	34.0
4	33.9019691389002	37.0	35.0	37.0	30.0	37.0
5	33.459959609950616	37.0	35.0	37.0	28.0	37.0
6	33.315109115806	37.0	35.0	37.0	27.0	37.0
7	33.154735640859506	37.0	35.0	37.0	26.0	37.0
8	33.09922705222526	37.0	35.0	37.0	26.0	37.0
9	34.42176310365832	39.0	35.0	39.0	26.0	39.0
10	34.33951898959047	39.0	35.0	39.0	26.0	39.0
11	34.31821479408424	39.0	35.0	39.0	26.0	39.0
12	34.17335663493807	39.0	35.0	39.0	25.0	39.0
13	34.16710109190202	39.0	35.0	39.0	25.0	39.0
14	35.22890895916944	39.0	35.0	41.0	25.0	41.0
15	35.233921419305794	40.0	35.0	41.0	25.0	41.0
16	35.229837777005265	40.0	35.0	41.0	25.0	41.0
17	35.21602138625611	40.0	35.0	41.0	25.0	41.0
18	35.13735141732642	40.0	35.0	41.0	25.0	41.0
19	35.14398434896859	40.0	35.0	41.0	24.0	41.0
20	35.11380227812864	40.0	35.0	41.0	24.0	41.0
21	35.078409358876726	40.0	35.0	41.0	24.0	41.0
22	35.03816764981584	40.0	35.0	41.0	24.0	41.0
23	34.873339456316934	40.0	35.0	41.0	22.0	41.0
24	34.82769572468757	40.0	35.0	41.0	22.0	41.0
25	34.76019140861537	40.0	35.0	41.0	21.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.654426573424626
1101	2	0.4473098592765865
1101	3	0.5522901422725397
1101	4	0.5193016493142935
1101	5	0.5694353837579342
1101	6	0.5940143289998829
1101	7	0.6485862695961444
1101	8	0.43509743230721654
1101	9	0.7978282735371423
1101	10	0.5001556786605832
1101	11	0.7573760063948569
1101	12	0.7827129893628495
1101	13	0.8984389435238072
1101	14	0.9952564663029548
1101	15	0.6918384364741499
1101	16	0.925132770028064
1101	17	1.0026943937300388
1101	18	0.9066493687114558
1101	19	0.7278739263970024
1101	20	0.7646364769552534
1101	21	0.8551573158701231
1101	22	0.7865956702562826
1101	23	0.8947155591589535
1101	24	0.9696763786527711
1101	25	1.0441236902739348
1102	1	0.8490779193506519
1102	2	1.0055417450189559
1102	3	0.7030824515804319
1102	4	0.8033160408781299
1102	5	0.9525687198672088
1102	6	0.9138434532019346
1102	7	0.9507010314732298
1102	8	0.9650297527890217
1102	9	1.0641900750358744
1102	10	0.9470544595507207
1102	11	1.1096490196502629
1102	12	1.1124984291378723
1102	13	1.162779282136455
1102	14	1.2275315111793574
1102	15	1.2844818636540367
1102	16	1.3689368064563112
1102	17	1.33136024561297
1102	18	0.6674570204184036
1102	19	1.1478816410659007
1102	20	1.3526438731538306
1102	21	1.2825574770270904
1102	22	1.0948304615827311
1102	23	1.2811577835643035
1102	24	1.326887878176663
1102	25	1.4609028663418115
1103	1	0.7745144995100368
1103	2	0.9424399530538814
1103	3	0.5840956516851925
1103	4	0.5462152956330115
1103	5	0.6010146597946999
1103	6	0.6638221155363695
1103	7	0.7708321772416156
1103	8	0.7980442398009657
1103	9	0.8563530584625099
1103	10	0.7587337626355293
1103	11	0.9608291055592986
1103	12	1.0648153273725
1103	13	1.1334555243852122
1103	14	1.1452500161867292
1103	15	1.23118857334169
1103	16	1.146855446801645
1103	17	1.1489481543577753
1103	18	1.134191666272983
1103	19	1.1323091778661762
1103	20	0.8855776625769991
1103	21	1.162743227720732
1103	22	1.040370357699537
1103	23	1.0776682443382342
1103	24	1.0776009284524548
1103	25	1.0039177832091113
1104	1	0.781762340129923
1104	2	0.8280873286260366
1104	3	0.7293546024360325
1104	4	0.5521941203713681
1104	5	0.7018955501223942
1104	6	0.5512494366035625
1104	7	0.5170909423422287
1104	8	0.6573917462330385
1104	9	0.7806463043958232
1104	10	0.7652845014347065
1104	11	0.8350978794169635
1104	12	0.8589667187835559
1104	13	0.7647104103181945
1104	14	0.97848608759125
1104	15	0.9497028947692243
1104	16	1.041473538877547
1104	17	0.8453864538434388
1104	18	0.40701972823596577
1104	19	0.8317127482315243
1104	20	0.8835153269022413
1104	21	0.8536178490908313
1104	22	0.8765592595044893
1104	23	0.7836400397708161
1104	24	0.9387523913745213
1104	25	0.8004299078045989
1105	1	0.6342415110005355
1105	2	0.6636776617360916
1105	3	0.7030512742157207
1105	4	0.46437197542837083
1105	5	0.5459606905168215
1105	6	0.6020333888516802
1105	7	0.6370422454604281
1105	8	0.5670956680258854
1105	9	0.8732495011016326
1105	10	0.5234276936521169
1105	11	0.9194234291470522
1105	12	0.9056137334250209
1105	13	1.0133610194492562
1105	14	1.1012740132196441
1105	15	1.0806630294691502
1105	16	0.9920741663273702
1105	17	0.8179479965765637
1105	18	0.7172101559486777
1105	19	0.9752523106762254
1105	20	1.0226873599010489
1105	21	0.9777578658271651
1105	22	0.8867048718218271
1105	23	1.0949049054437836
1105	24	1.0442034110104927
1105	25	0.7921814919876766
1106	1	0.16743211108031275
1106	2	0.4706922127617581
1106	3	-1.3982224700434713
1106	4	-0.10613263310244747
1106	5	0.09453674098296716
1106	6	0.1870272464973226
1106	7	0.36272902421917763
1106	8	0.443193525615051
1106	9	0.6356680884307337
1106	10	0.48746438539418335
1106	11	0.6103210071531819
1106	12	0.7915097052407916
1106	13	0.6364070270954301
1106	14	0.647484198231588
1106	15	0.6343269967946483
1106	16	0.7584731934349236
1106	17	0.7251785890313514
1106	18	0.8026602775602356
1106	19	0.834438748781416
1106	20	0.8605892163747058
1106	21	0.7352207381191462
1106	22	0.7267667013256016
1106	23	0.710327068752747
1106	24	0.7434285565383973
1106	25	0.8708587853980632
1107	1	0.6466461699152575
1107	2	0.7501417713805232
1107	3	0.6057357476819085
1107	4	0.46777336103517086
1107	5	0.5808945202208591
1107	6	0.47617731952183107
1107	7	0.5094249631032852
1107	8	0.40550377253661907
1107	9	0.49651971760921754
1107	10	0.5270438391231949
1107	11	0.5719062858208375
1107	12	0.7281411987196549
1107	13	0.5529563552359065
1107	14	0.667658282531491
1107	15	0.5466512017807403
1107	16	0.6289619299577325
1107	17	0.6700455108015078
1107	18	0.67465061245165
1107	19	0.6337492714598625
1107	20	0.6676751469860989
1107	21	0.5988862790622349
1107	22	0.6592739271773027
1107	23	0.7658392275887138
1107	24	0.8795591767871258
1107	25	0.904435164948481
1108	1	0.6742869648731329
1108	2	0.6068778528666314
1108	3	0.5756237191162157
1108	4	0.5094124430531508
1108	5	0.7733440107053795
1108	6	0.7589432764086652
1108	7	0.7319244341399767
1108	8	0.5926197657151633
1108	9	0.7483663837287793
1108	10	0.7690756739012983
1108	11	0.7848360869473794
1108	12	0.7646521480008275
1108	13	0.772016866962943
1108	14	0.9260221434077209
1108	15	0.8562302745967969
1108	16	0.749348634232831
1108	17	0.8189078668539054
1108	18	0.9791342513555037
1108	19	1.0031738925741962
1108	20	1.0410502646320836
1108	21	0.993824756223745
1108	22	0.9949125276452762
1108	23	1.0905340099808285
1108	24	1.0876069636469339
1108	25	1.0357660860655926
1109	1	0.3538791723096715
1109	2	0.2744936987623383
1109	3	0.429975328221726
1109	4	0.3182606969362354
1109	5	-0.04961426189700546
1109	6	0.241710619309643
1109	7	0.33168362621982794
1109	8	0.41570123679925075
1109	9	0.5416985753004226
1109	10	0.1857372505464454
1109	11	0.37518484723771195
1109	12	0.5529337607272353
1109	13	0.4960592143451308
1109	14	0.586927544459499
1109	15	0.5147307901860358
1109	16	0.4228713802415598
1109	17	0.5737458424864741
1109	18	0.6911186371655589
1109	19	0.6100232650643704
1109	20	0.6651665071502535
1109	21	0.7360101759269568
1109	22	0.7429606141764395
1109	23	0.6477646550242824
1109	24	0.7024408433092404
1109	25	0.6961450790876711
1110	1	0.27009220661753375
1110	2	0.10112231540199446
1110	3	0.16682287478933944
1110	4	0.12361931686002237
1110	5	0.18008017204165583
1110	6	0.24913005841557379
1110	7	0.14931215472948622
1110	8	0.23576910875229373
1110	9	0.1578573484044128
1110	10	0.33765795093884066
1110	11	0.2925273682219327
1110	12	0.36535352982664904
1110	13	0.3789731002732992
1110	14	0.39239468679102885
1110	15	0.4011878181185011
1110	16	0.30269787085373423
1110	17	0.4619408097499331
1110	18	0.38290859690635415
1110	19	0.4749659035025644
1110	20	0.5239703183180993
1110	21	0.4331054033517461
1110	22	0.46303911705726364
1110	23	0.46685519298479505
1110	24	0.5962769997664665
1110	25	0.5783816682117973
1111	1	0.118344446390374
1111	2	-0.04175378503790128
1111	3	0.011122258536772733
1111	4	0.16584861035691745
1111	5	0.23762982601798655
1111	6	0.3818363269169964
1111	7	0.1854917902051696
1111	8	0.27823860571272263
1111	9	0.30383563612708997
1111	10	0.20100652723548507
1111	11	0.13076939312468738
1111	12	0.3859216514666812
1111	13	0.34638368204484493
1111	14	0.177423554766861
1111	15	0.3041991356842786
1111	16	0.15847856011321682
1111	17	0.3241535534771103
1111	18	0.2504881145205502
1111	19	-8.424651612131129E-4
1111	20	0.3448749114937115
1111	21	0.48802549836279496
1111	22	0.15300170816811942
1111	23	0.2890756935414345
1111	24	0.37642759053630215
1111	25	0.3613664731647219
1112	1	0.08356193650445931
1112	2	-0.3218255139642281
1112	3	-0.002424883669291944
1112	4	0.11834370578869624
1112	5	0.07253981782425711
1112	6	0.05016113893413632
1112	7	0.1430749528869626
1112	8	0.14942412922086845
1112	9	0.09452497211550082
1112	10	-0.2715541487534381
1112	11	-0.04587537240808359
1112	12	0.2802076298681513
1112	13	0.031336884417711985
1112	14	-0.11603346868538722
1112	15	-0.34214064985372517
1112	16	-0.31711376554274295
1112	17	-0.09053905263293416
1112	18	0.11538015873966856
1112	19	0.03914189672897095
1112	20	-0.08939051501639028
1112	21	0.1296523531617808
1112	22	0.17344779968484403
1112	23	0.2466200066377695
1112	24	0.17967348107936232
1112	25	0.26278971928252304
1113	1	-0.6733652246038879
1113	2	-0.6376037971821376
1113	3	-0.5335433160853356
1113	4	-2.2124366549087604
1113	5	-0.8603895242123798
1113	6	-0.48572946580183185
1113	7	-0.5754616695905455
1113	8	-0.4311478263360655
1113	9	-0.3731048502542009
1113	10	-0.48343232170334005
1113	11	-0.684086986469751
1113	12	-0.2899389658101015
1113	13	-0.33296465644276196
1113	14	-0.5370846068457382
1113	15	-0.638975211196275
1113	16	-0.9292913213103446
1113	17	-1.065044118451226
1113	18	-0.8062861051108996
1113	19	-0.5938649622781327
1113	20	-0.3449191079233316
1113	21	-0.43675454737945074
1113	22	-0.3614233169476577
1113	23	-0.1787205430569827
1113	24	-0.18525911255682104
1113	25	-0.47483644295014926
1114	1	-1.0159690049043633
1114	2	-2.255897093773136
1114	3	-1.4758437847955683
1114	4	-1.0256977599693613
1114	5	-0.9774185918691742
1114	6	-0.6407772896194999
1114	7	-0.6129934509348942
1114	8	-0.7718278502108333
1114	9	-1.0008602831199838
1114	10	-1.132717031517707
1114	11	-0.9957412603500089
1114	12	-1.2341287934544454
1114	13	-0.9928244148011913
1114	14	-1.1498583979596262
1114	15	-1.2724868947160957
1114	16	-1.6509571546856705
1114	17	-1.803463629612807
1114	18	-1.4302335095698524
1114	19	-1.9894064014045796
1114	20	-1.244058637714467
1114	21	-1.444092598175338
1114	22	-1.0954946944880675
1114	23	-0.9617941349560937
1114	24	-1.0445905706616472
1114	25	-1.7248466168561123
1115	1	-1.142240894938812
1115	2	-1.188491594228644
1115	3	-0.7383196473145581
1115	4	-0.580915653001675
1115	5	-0.8674724450600735
1115	6	-0.800541201614621
1115	7	-0.6535574855813664
1115	8	-1.076535514272848
1115	9	-2.0131937598422027
1115	10	-1.515653337076678
1115	11	-2.0192259682104634
1115	12	-2.6563025224988692
1115	13	-2.6321634881246503
1115	14	-2.0638821635587092
1115	15	-2.298400476198786
1115	16	-2.097464442177639
1115	17	-1.9108526130384078
1115	18	-2.474855697806724
1115	19	-2.8209248833660894
1115	20	-2.852185445649802
1115	21	-2.275174182227623
1115	22	-2.2014340036112756
1115	23	-2.287461970273853
1115	24	-2.742306078454696
1115	25	-2.7199850684335374
1116	1	-1.4716078977522677
1116	2	-1.583445644509247
1116	3	-1.649613256557224
1116	4	-1.3482906120974647
1116	5	-1.6033037619221204
1116	6	-2.5017343290143472
1116	7	-2.0010156451800682
1116	8	-2.326150172200265
1116	9	-3.3452442205991986
1116	10	-1.94692402736338
1116	11	-2.941788116627162
1116	12	-5.270773447990688
1116	13	-4.306807066391926
1116	14	-3.5489803541359564
1116	15	-4.491680469496636
1116	16	-3.9561550791054074
1116	17	-3.777814482912781
1116	18	-3.7621277404913513
1116	19	-4.066491087401804
1116	20	-5.983584730270987
1116	21	-4.755033405603509
1116	22	-4.0834863383244056
1116	23	-4.7712097313687
1116	24	-4.673155994725075
1116	25	-4.016051398292024
1201	1	0.5153698744035466
1201	2	0.7884817759223779
1201	3	0.7582395744535333
1201	4	0.6410013014086076
1201	5	0.07462580891112225
1201	6	0.6845898897081995
1201	7	0.7717670166798172
1201	8	0.6373597611091739
1201	9	0.6543668096961639
1201	10	0.7325083649935564
1201	11	0.5309193514506205
1201	12	0.9944016410823195
1201	13	0.9858046174568713
1201	14	0.9386014278864039
1201	15	0.7596891817307068
1201	16	0.702589536106224
1201	17	0.626411126543367
1201	18	0.9489478819655872
1201	19	1.0951657056887996
1201	20	1.0656671970088922
1201	21	0.7849675782403693
1201	22	0.8140477272698732
1201	23	0.8292734062499747
1201	24	0.6241440565753535
1201	25	0.7811052929004632
1202	1	1.0369938826081864
1202	2	1.0626353019561954
1202	3	1.0286051179185165
1202	4	0.890779154058265
1202	5	1.021304706023578
1202	6	1.0385678082615755
1202	7	1.1723688508764383
1202	8	1.0371278022508648
1202	9	1.3159323561231133
1202	10	1.312746321830332
1202	11	1.2882374405391133
1202	12	1.3780645546929193
1202	13	1.3813688902390098
1202	14	1.5820962129773548
1202	15	1.4621085514986802
1202	16	1.4977556097628835
1202	17	1.363893678720224
1202	18	1.429875050956781
1202	19	1.459935727644364
1202	20	1.3249390720436054
1202	21	1.4041759191328822
1202	22	1.348870990540398
1202	23	1.4817744151691912
1202	24	1.5045973112191575
1202	25	1.5708709644704157
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2211	10	-0.18883829951307263
2211	11	-0.03390353740852703
2211	12	-0.14181475217555572
2211	13	5.57774747342421E-4
2211	14	-0.10939924829850156
2211	15	-0.11222695725346199
2211	16	-0.015495240576669289
2211	17	-0.22139127250434143
2211	18	-0.22167575774055592
2211	19	-0.07835813335101705
2211	20	-0.14918351404179475
2211	21	-0.054219802458781885
2211	22	-0.13953605274463854
2211	23	-0.1980419987918296
2211	24	0.012651026210427574
2211	25	-0.21036104224776864
2212	1	-0.3347851928935768
2212	2	-0.24012822722064442
2212	3	-0.16852681223533494
2212	4	-0.21402423346938093
2212	5	-0.5325424503933505
2212	6	-0.22613224115609398
2212	7	-0.42263658199281906
2212	8	-0.12801673291127003
2212	9	-0.42491790115455075
2212	10	-0.5481834990959484
2212	11	-0.3807162525564749
2212	12	-0.14548955133830788
2212	13	-0.22646540801722637
2212	14	-0.12729635619670887
2212	15	-0.38350956314451423
2212	16	-0.3245606117546984
2212	17	-0.4065548263738279
2212	18	-0.4468264039521159
2212	19	-0.24604329828599703
2212	20	-0.3717536848082901
2212	21	-0.3110770769736604
2212	22	-0.24979806325209353
2212	23	-0.5681801386787342
2212	24	-0.6187906571405506
2212	25	-0.36586151783581755
2213	1	-0.37094128130880577
2213	2	-0.021084844878583198
2213	3	-0.12346966455507769
2213	4	-0.29473239471212054
2213	5	-0.22284899081437004
2213	6	-0.07160542923088542
2213	7	-0.5062441704143339
2213	8	-0.5112270233599361
2213	9	-0.2959506292731362
2213	10	-0.2859229243845647
2213	11	-0.12035092170897599
2213	12	-0.4161247110076758
2213	13	-0.3114691318404823
2213	14	-0.26353339659967645
2213	15	-0.6826957315555191
2213	16	-1.1190656296483041
2213	17	-0.7588494286041154
2213	18	-0.4378476225054584
2213	19	-0.43591365577780294
2213	20	-0.289007484086504
2213	21	-0.34011923898996343
2213	22	-0.4586164840296476
2213	23	-0.7311760431891159
2213	24	-0.3603823024117361
2213	25	-0.4948900446607922
2214	1	-1.0587805657138176
2214	2	-0.4305117334579016
2214	3	-0.5597616703228496
2214	4	-0.5343227850144388
2214	5	-0.7720417468815342
2214	6	-1.363731440424278
2214	7	-0.8352917545480665
2214	8	-0.5998386028990055
2214	9	-0.4387015640923977
2214	10	-0.785287491679334
2214	11	-0.8837832359060087
2214	12	-0.5709811276560117
2214	13	-0.8676736434126937
2214	14	-0.5464070188104913
2214	15	-0.5552792542966714
2214	16	-0.6921379467781037
2214	17	-0.8416443549183583
2214	18	-0.8498357936812511
2214	19	-0.7585375040560223
2214	20	-1.0361839633005232
2214	21	-1.2385604736845863
2214	22	-1.7018791865738194
2214	23	-1.7034384280169377
2214	24	-1.288490423730508
2214	25	-1.7668826149860806
2215	1	-1.8178917570061408
2215	2	-1.1906873303067798
2215	3	-1.2023006083227301
2215	4	-1.3396905160866623
2215	5	-1.8555861572489611
2215	6	-1.785245662566318
2215	7	-2.108003282601466
2215	8	-1.8636283677676104
2215	9	-1.9365198333809914
2215	10	-2.0989984785095643
2215	11	-1.869367456243971
2215	12	-1.925300689866603
2215	13	-2.037501053359172
2215	14	-2.2202310611461655
2215	15	-2.1761885092127855
2215	16	-2.1876157265604093
2215	17	-2.385565216064549
2215	18	-1.9845678882148974
2215	19	-2.2676180260356986
2215	20	-2.2295605944870047
2215	21	-2.0074557770702413
2215	22	-2.1276738825868122
2215	23	-2.5311237969507303
2215	24	-2.597576959300895
2215	25	-2.963115701762195
2216	1	-1.6488779051038271
2216	2	-1.0579500360162548
2216	3	-1.0959121432546333
2216	4	-1.3080081133136545
2216	5	-2.201338346885464
2216	6	-2.5065493666036573
2216	7	-2.4417228402263937
2216	8	-2.4324211447352795
2216	9	-4.317948412852552
2216	10	-2.3288758738790065
2216	11	-2.477489801890318
2216	12	-2.9508065066896947
2216	13	-2.763652641701878
2216	14	-2.914163886645227
2216	15	-2.8510120527549745
2216	16	-2.124810552130782
2216	17	-2.404498463820488
2216	18	-2.804678575817995
2216	19	-2.9125716809974023
2216	20	-2.33838088383618
2216	21	-2.836596381104016
2216	22	-2.269459860280385
2216	23	-3.414929588813745
2216	24	-2.853511169802516
2216	25	-2.8468133958642987
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68770.0
3	2.0
4	1153.0
5	9960.0
6	11629.0
7	8711.0
8	7042.0
9	5778.0
10	5191.0
11	4689.0
12	4163.0
13	3944.0
14	3772.0
15	3979.0
16	3937.0
17	4295.0
18	4595.0
19	5447.0
20	7054.0
21	9441.0
22	11889.0
23	14120.0
24	16704.0
25	19077.0
26	21355.0
27	23232.0
28	26373.0
29	31379.0
30	38809.0
31	49641.0
32	63398.0
33	80913.0
34	105537.0
35	138159.0
36	188380.0
37	291064.0
38	924291.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78357577521609	18.927705161789625	13.256663485251547	27.03205557774273
2	13.527100662179961	20.126166032152717	40.47678761524999	25.869945690417335
3	17.66238381345755	24.992840866095108	29.253235815782748	28.0915395046646
4	11.733791584637459	16.57816680840472	36.581224021579054	35.106817585378764
5	12.957667335764695	38.776538245243444	34.552607024492055	13.713187394499807
6	32.47976959192705	36.14260469577355	17.761840377264345	13.615785335035046
7	28.831857073300384	31.36945398459347	21.219743212430274	18.578945729675873
8	26.157136089869393	36.57237598971375	19.85427804470912	17.416209875707743
9	27.54993708536096	14.23593666142002	19.514993528135697	38.699132725083324
10	16.269550377959312	28.670323934836812	32.859431254350355	22.20069443285352
11	35.022097247730414	21.72596166203048	24.250156806813557	19.001784283425554
12	24.463751335182863	24.29669848561294	30.682220391629595	20.557329787574606
13	29.68906267895422	21.48753734647701	25.45602607331949	23.367373901249287
14	21.36146985395335	22.076846391532712	26.902459270294287	29.65922448421965
15	23.576283652430444	31.10438098828487	22.578610084331466	22.740725274953224
16	22.453556944205204	27.30339429543366	27.594556789988733	22.6484919703724
17	21.864066918182505	28.402216116943592	27.27305520497549	22.46066175989842
18	21.84027303384922	26.40224516141832	30.029124151672846	21.728357653059614
19	24.094259831808344	26.02678207091897	27.120104498682718	22.75885359858997
20	24.713255749989855	26.21810508469991	27.649864958089665	21.418774207220572
21	23.665541561122268	25.752322277360307	26.57915311384908	24.002983047668344
22	24.305985462543347	27.413264245620883	26.491067728893253	21.789682562942517
23	22.638942806914553	27.14569319343353	27.672188623965393	22.543175375686523
24	24.046282181170877	25.868613757415325	27.98140380445589	22.103700256957907
25	22.947401833829908	26.75776600272694	27.947735486989327	22.347096676453827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	90.0
1	90.0
2	289.5
3	489.0
4	489.0
5	489.0
6	1233.5
7	1978.0
8	1978.0
9	1978.0
10	2391.5
11	2805.0
12	2805.0
13	2805.0
14	4191.0
15	5577.0
16	5577.0
17	5577.0
18	11704.0
19	17831.0
20	17831.0
21	17831.0
22	31248.0
23	44665.0
24	44665.0
25	44665.0
26	70018.0
27	95371.0
28	95371.0
29	95371.0
30	124832.0
31	154293.0
32	154293.0
33	154293.0
34	176372.5
35	198452.0
36	198452.0
37	198452.0
38	213999.0
39	229546.0
40	229546.0
41	229546.0
42	246955.0
43	264364.0
44	264364.0
45	264364.0
46	282565.0
47	300766.0
48	300766.0
49	300766.0
50	292553.0
51	284340.0
52	284340.0
53	284340.0
54	255198.5
55	226057.0
56	226057.0
57	226057.0
58	196901.0
59	167745.0
60	167745.0
61	167745.0
62	140145.0
63	112545.0
64	112545.0
65	112545.0
66	87240.5
67	61936.0
68	61936.0
69	61936.0
70	46033.0
71	30130.0
72	30130.0
73	30130.0
74	21299.0
75	12468.0
76	12468.0
77	12468.0
78	8463.5
79	4459.0
80	4459.0
81	4459.0
82	2958.5
83	1458.0
84	1458.0
85	1458.0
86	933.0
87	408.0
88	408.0
89	408.0
90	251.0
91	94.0
92	94.0
93	94.0
94	50.0
95	6.0
96	6.0
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08467572309144843
2	0.04296909696813118
3	0.019207592138954756
4	0.0075297368244259255
5	0.020740592450514524
6	0.0578933058836101
7	0.0811588400237525
8	0.05965174741745807
9	0.02628644651880428
10	0.049597068903404304
11	0.007935531024544688
12	5.410589334916832E-4
13	0.005726207046120315
14	9.017648891528053E-4
15	0.014157708759699045
16	0.009513619580562098
17	0.0054105893349168325
18	0.0056811188016626735
19	0.0020740592450514523
20	0.0019387945116785316
21	3.156177112034819E-4
22	7.214119113222443E-4
23	0.0
24	0.0
25	4.508824445764027E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2217873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.548175895063515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.78372401748221	35.56752584020904
2	14.786347472314587	14.652730908075421
3	5.499003713661761	8.173968097563614
4	2.6623753297535204	5.276633645492207
5	1.5038176476455996	3.725571065982241
6	0.9059027497333613	2.693149727256617
7	0.6046885120809742	2.097284893081864
8	0.41516846832207216	1.645667223560491
9	0.299080448175413	1.3337001587672814
>10	1.4048802869896337	11.864334881504792
>50	0.07744871976748166	2.662164158113945
>100	0.04871807191258471	4.677977063181948
>500	0.005379682139084338	1.9218954585030403
>1k	0.0032825179153734537	3.1543662649901516
>5k	1.823621064096363E-4	0.5530306137173366
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6699	0.3020461496217322	No Hit
GTCCTACAGTGGACATTTCTAAATT	5542	0.24987905078424238	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4935	0.22251048639845475	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4784	0.21570216148535104	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4610	0.20785680694972164	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4425	0.19951548172505823	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3924	0.17692627125178043	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3519	0.15866553224643612	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2904	0.13093626190498736	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2403	0.10834705143170957	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2356	0.10622790394220047	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2336	0.10532613905304766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.5088244457640273E-5	0.0
10	0.0	0.0	0.0	4.5088244457640273E-5	0.0
11	0.0	0.0	0.0	4.5088244457640273E-5	0.0
12	0.0	0.0	0.0	4.5088244457640273E-5	4.5088244457640273E-5
13	0.0	0.0	0.0	4.5088244457640273E-5	4.5088244457640273E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1695	0.0	13.131011	1
TAGGACC	1230	0.0	12.602481	4
GACGTTG	55	0.0030547178	12.098523	7
CTACACT	285	0.0	12.01244	4
GGCGAGG	1230	0.0	11.807721	19
TGTAGGA	2885	0.0	10.747662	2
TAGGGTG	170	1.364242E-10	10.628654	5
TAGGACA	845	0.0	10.579002	4
GTCCTAC	3075	0.0	10.578718	1
ACACCGT	90	9.4188945E-5	10.566497	6
TAGAAAT	750	0.0	10.524231	4
GTATCAA	3690	0.0	10.516853	1
TGGCGAG	2895	0.0	10.492553	18
GACGTGA	1550	0.0	10.487246	7
GTAGGAC	2940	0.0	10.481898	3
CTGTAGG	2945	0.0	10.205958	1
GGACGTG	2705	0.0	10.160243	6
GTCCTAT	225	0.0	10.145671	1
TTAGGAC	1800	0.0	10.145442	3
ATAGGAC	300	0.0	10.145441	3
>>END_MODULE