FastQCFastQC Report
Thu 26 May 2016
SRR1512846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336592
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49750.21291693201038092No Hit
GTCCTACAGTGGACATTTCTAAATT29880.12787855132603382No Hit
TATCAACGCAGAGTACTTTTTTTTT28160.12051740312386586No Hit
GGTATCAACGCAGAGTACTTTTTTT27840.11914788717927649No Hit
GTCCTACAGTGTGCATTTCTCATTT26760.11452577086628732No Hit
GTCCTAAAGTGTGTATTTCTCATTT25860.11067400727212966No Hit
GTACTGGTTCACTATCGGTCAGTCA24960.10682224367797202No Hit
GTACATGGGAAGCAGTGGTATCAAC23970.10258530372439861No Hit
CTGTAGGACGTGGAATATGGCAAGA23740.10160096413922498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC400.005285013514.24802411
TAGGACC7200.012.2691324
GTCCTAT3150.011.7890631
GTCCTAC19150.011.2870991
GCGCCAC1303.2752723E-810.96001813
TCCTACA20000.010.8284982
AGGACCT11350.010.7958595
TGGCGAG14350.010.65751518
TTAGACA2050.010.6570594
TGTAGGA18550.010.6508142
ATAAGAC1909.094947E-1210.4985433
TCCTATA3350.010.4910822
CGTGCGC1451.4475518E-810.48130410
CATTCCG1002.4034644E-510.4485519
CCTACAG20000.010.3535643
GGTATCA21700.010.3117731
GTAGGAC17500.010.2042983
GCATTTC15450.010.14422413
TGTACGC750.00265147110.1319282
ACGAAAT1502.451452E-810.13192812
Produced by FastQC (version 0.11.5)