FastQCFastQC Report
Thu 26 May 2016
SRR1512846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336592
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62370.2669272170751248No Hit
TATCAACGCAGAGTACTTTTTTTTT43670.18689612906318262No Hit
GGTATCAACGCAGAGTACTTTTTTT35310.15111752501078493No Hit
GTCCTACAGTGGACATTTCTAAATT28850.12347042187938673No Hit
GTCCTACAGTGTGCATTTCTCATTT24750.10592349883933525No Hit
GTCCTAAAGTGTGTATTTCTCATTT24600.10528153824030896No Hit
CTGTAGGACGTGGAATATGGCAAGA23700.10142977464615133No Hit
GTACTGGTTCACTATCGGTCAGTCA23680.10134417989961447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC801.2725468E-714.2653096
GACCGCG852.6798443E-713.4204187
TAGGACC7750.012.5166584
CGTGCGC1405.2750693E-1112.2101310
TACGCTC550.003044655312.1038994
ACGATAC657.945517E-411.7063743
TGGATCG752.0540786E-411.4122475
GAACCGA752.0540786E-411.4122476
GCGTGCG1408.381903E-910.8550759
TGGCGAG16750.010.82381918
GGCGAGG7550.010.68600519
GTAGGAC17650.010.6161573
TGTAGGA17900.010.5212492
GGTATCA21400.010.4894651
GTCCTAA7650.010.4441461
AGGACCT12850.010.2873055
TATGGCG18100.010.1742816
GGGTATA750.002621670710.1457421
GTCCTAC18050.010.1176391
GTACTAG1803.8562575E-1010.0400571
Produced by FastQC (version 0.11.5)