##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512846_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2336592 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.32818438135541 31.0 30.0 34.0 26.0 34.0 2 30.315875856803412 31.0 30.0 34.0 26.0 34.0 3 30.507223340660243 31.0 31.0 34.0 27.0 34.0 4 34.131981107527544 37.0 35.0 37.0 30.0 37.0 5 33.742506607914436 37.0 35.0 37.0 28.0 37.0 6 33.63506465827153 37.0 35.0 37.0 28.0 37.0 7 33.46971529475407 37.0 35.0 37.0 28.0 37.0 8 33.41848298718818 37.0 35.0 37.0 27.0 37.0 9 34.77526799715141 39.0 35.0 39.0 27.0 39.0 10 34.68445881865554 39.0 35.0 39.0 27.0 39.0 11 34.690062706711316 39.0 35.0 39.0 27.0 39.0 12 34.564256404198936 39.0 35.0 39.0 27.0 39.0 13 34.561928227093134 39.0 35.0 39.0 27.0 39.0 14 35.64321456206304 40.0 36.0 41.0 27.0 41.0 15 35.63649794230229 40.0 36.0 41.0 27.0 41.0 16 35.62731619384129 40.0 36.0 41.0 27.0 41.0 17 35.60392571745516 40.0 36.0 41.0 27.0 41.0 18 35.52600240007669 40.0 36.0 41.0 27.0 41.0 19 35.531205704718666 40.0 36.0 41.0 26.0 41.0 20 35.50176025596253 40.0 36.0 41.0 26.0 41.0 21 35.46713632504091 40.0 36.0 41.0 26.0 41.0 22 35.429941555907064 40.0 36.0 41.0 26.0 41.0 23 35.279896533070385 40.0 35.0 41.0 25.0 41.0 24 35.231600981258175 40.0 35.0 41.0 25.0 41.0 25 35.16744900265002 40.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.75469389066226 1101 2 0.6565040655675389 1101 3 0.6459448912941177 1101 4 0.6582947269391681 1101 5 0.7488624168776141 1101 6 0.782701799278783 1101 7 0.8301331865059254 1101 8 0.5372705666150424 1101 9 0.9458615660374292 1101 10 0.7187136944890611 1101 11 1.0113234352325051 1101 12 1.050660050938653 1101 13 1.0998480555184074 1101 14 1.1694223407743465 1101 15 0.8885259612234719 1101 16 1.0806708647904628 1101 17 1.1904532829789858 1101 18 1.0499580641269972 1101 19 0.8779015284410434 1101 20 1.0709653591533765 1101 21 1.0426192539464125 1101 22 1.0987066898623894 1101 23 1.2178280837312698 1101 24 1.2334696519119888 1101 25 1.1910431235141274 1102 1 0.8339891535746169 1102 2 0.8455756825167917 1102 3 0.5243345700515967 1102 4 0.7303483925958858 1102 5 0.8170917563394795 1102 6 0.7174131731667615 1102 7 0.7489890378685473 1102 8 0.8035927231430477 1102 9 0.9591194938353169 1102 10 0.9589493148160386 1102 11 1.038083611793951 1102 12 1.0529644019171442 1102 13 1.0621445554155073 1102 14 1.1695975253516622 1102 15 1.2289643767404854 1102 16 1.2499911530247587 1102 17 1.2852112946340384 1102 18 0.6530571173832556 1102 19 1.12346812091792 1102 20 1.241135790603984 1102 21 1.1394850465943733 1102 22 0.8665417175499002 1102 23 1.1453565969986457 1102 24 1.183989676197605 1102 25 1.1655747867349646 1103 1 0.8105987156475116 1103 2 0.9762904502127121 1103 3 0.6799764015496095 1103 4 0.6341075474798288 1103 5 0.830753611306676 1103 6 0.8089882146886254 1103 7 0.853657658891926 1103 8 0.8987068783298682 1103 9 0.9943484874355235 1103 10 0.8314328927257009 1103 11 0.9962925438896804 1103 12 1.0615789733791203 1103 13 1.0278388333577197 1103 14 1.17292335962766 1103 15 1.0877159456963597 1103 16 1.0998372509557797 1103 17 1.1018713168635514 1103 18 1.189821469015321 1103 19 1.181225559987496 1103 20 1.124379694039419 1103 21 1.213233931948956 1103 22 1.0910470122036884 1103 23 1.1434260945242372 1103 24 1.1947825430304064 1103 25 1.2054645125568442 1104 1 0.7105437693819283 1104 2 0.8403752850434145 1104 3 0.7847130726046316 1104 4 0.5988674364305382 1104 5 0.7266983290288422 1104 6 0.6706417805497793 1104 7 0.6526216670223164 1104 8 0.6538816047002172 1104 9 0.8047290626223926 1104 10 0.8286310463108393 1104 11 1.0439707543214851 1104 12 1.0094779328428274 1104 13 0.7239660167487969 1104 14 1.1447233937797208 1104 15 1.0188763998086543 1104 16 0.9792379097601227 1104 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2216 20 -2.8698294140126634 2216 21 -3.897966399130368 2216 22 -2.913605888204728 2216 23 -4.482733853491812 2216 24 -3.6068985084835106 2216 25 -3.8180447409978626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59556.0 3 0.0 4 950.0 5 8219.0 6 9594.0 7 7679.0 8 6181.0 9 5376.0 10 4574.0 11 4218.0 12 3868.0 13 3603.0 14 3599.0 15 3702.0 16 3825.0 17 3906.0 18 4554.0 19 5257.0 20 6943.0 21 9227.0 22 11822.0 23 14646.0 24 17127.0 25 20004.0 26 22004.0 27 24686.0 28 28311.0 29 33735.0 30 42739.0 31 54305.0 32 68802.0 33 87941.0 34 114552.0 35 149728.0 36 203303.0 37 312563.0 38 975493.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.278021095465014 18.756245649729614 13.008984312255716 26.95674894254966 2 14.371559244153337 20.080141941748735 38.97776422906946 26.570534585028465 3 17.813081343833655 24.709889897276838 28.861407202007378 28.615621556882125 4 12.16895459887642 16.31603681121285 35.962450919381524 35.5525576705292 5 13.577053898818466 38.25119279077512 34.120322689179226 14.051430621227182 6 33.22410862748137 35.53469932730884 17.499348034704678 13.7418440105051 7 29.492724386731805 31.08747439808188 20.85852836191411 18.561272853272207 8 26.970545501105033 35.28660358363142 19.69048859876269 18.052362316500865 9 27.63676323026291 14.27101277050421 18.994969195310976 39.097254803921906 10 16.19402723053986 28.309339549366165 32.60659756982118 22.890035650272793 11 35.53965480787421 21.942332730421267 23.31279494683788 19.20521751486665 12 24.258326824518793 24.236286016926485 30.165006473149802 21.34038068540492 13 29.658636841438486 20.973816754948523 25.208135740625977 24.159410662987018 14 22.208046659807614 21.03902640278151 26.546453522510756 30.20647341490012 15 24.452297455033882 29.294740009562297 22.566676040627346 23.68628649477648 16 23.57734190619107 27.128412243629636 25.879250083248944 23.414995766930353 17 22.790228870413763 27.498090071582194 26.508351255630814 23.203329802373233 18 22.680009963816964 26.24233132772997 28.369443609846375 22.708215098606694 19 24.33955480991042 25.909018929780487 26.760666458380587 22.9907598019285 20 24.947219077740854 25.621719247863833 26.9485929010569 22.48246877333841 21 24.39710192935086 25.45291448346286 26.262727420183868 23.887256167002413 22 24.629083801574865 26.317535165727328 26.14052424506084 22.91285678763697 23 23.64743181522491 26.117097037052257 26.720582797510218 23.51488835021262 24 24.169730958592687 25.688524141142317 26.857876770955308 23.283868129309695 25 23.57525282569899 26.022023547134925 27.006278380032438 23.39644524713364 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 100.0 1 100.0 2 249.5 3 399.0 4 399.0 5 399.0 6 1044.5 7 1690.0 8 1690.0 9 1690.0 10 2159.5 11 2629.0 12 2629.0 13 2629.0 14 3958.5 15 5288.0 16 5288.0 17 5288.0 18 11465.0 19 17642.0 20 17642.0 21 17642.0 22 29427.0 23 41212.0 24 41212.0 25 41212.0 26 63242.5 27 85273.0 28 85273.0 29 85273.0 30 111357.5 31 137442.0 32 137442.0 33 137442.0 34 161032.0 35 184622.0 36 184622.0 37 184622.0 38 205598.0 39 226574.0 40 226574.0 41 226574.0 42 248252.0 43 269930.0 44 269930.0 45 269930.0 46 293192.0 47 316454.0 48 316454.0 49 316454.0 50 316894.5 51 317335.0 52 317335.0 53 317335.0 54 289649.5 55 261964.0 56 261964.0 57 261964.0 58 229089.0 59 196214.0 60 196214.0 61 196214.0 62 165068.0 63 133922.0 64 133922.0 65 133922.0 66 104606.0 67 75290.0 68 75290.0 69 75290.0 70 56496.5 71 37703.0 72 37703.0 73 37703.0 74 26834.5 75 15966.0 76 15966.0 77 15966.0 78 11091.0 79 6216.0 80 6216.0 81 6216.0 82 4105.5 83 1995.0 84 1995.0 85 1995.0 86 1289.0 87 583.0 88 583.0 89 583.0 90 358.5 91 134.0 92 134.0 93 134.0 94 74.5 95 15.0 96 15.0 97 15.0 98 7.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08418243321897875 2 0.042198210042660424 3 0.017546923040051496 4 0.005563658524894376 5 0.020285954929230264 6 0.05756246704602258 7 0.07729205612276341 8 0.05833281976485411 9 0.024993665988756276 10 0.04818984230023898 11 0.006590795483336415 12 7.275553455631107E-4 13 0.005820442764504886 14 8.559474653683656E-4 15 0.014251525298383288 16 0.009672206358662531 17 0.005007292672404938 18 0.005906037511041722 19 0.0026534371426419332 20 0.002011476543615659 21 1.2839211980525484E-4 22 5.563658524894376E-4 23 0.0 24 0.0 25 8.559474653683656E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2336592.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.75772787440807 #Duplication Level Percentage of deduplicated Percentage of total 1 70.85343607952194 35.2550599141162 2 15.27876221149254 15.204729847544698 3 5.727159896428585 8.549113908591499 4 2.771851752704674 5.516841808771209 5 1.4847442661625778 3.693875057940263 6 0.9363976185261531 2.7955810730920865 7 0.6116581183950676 2.1304302745091976 8 0.41206969787203634 1.6402921513605069 9 0.31647802440464406 1.4172504674900899 >10 1.4644368596247477 12.823236472100799 >50 0.08993147597106838 3.0800307152344106 >100 0.04668312451880591 4.199311346069242 >500 0.0042317951956970315 1.4561180712283492 >1k 0.0020727160142186216 1.9701103465700727 >5k 8.636316725910922E-5 0.268018545381441 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6237 0.2669272170751248 No Hit TATCAACGCAGAGTACTTTTTTTTT 4367 0.18689612906318262 No Hit GGTATCAACGCAGAGTACTTTTTTT 3531 0.15111752501078493 No Hit GTCCTACAGTGGACATTTCTAAATT 2885 0.12347042187938673 No Hit GTCCTACAGTGTGCATTTCTCATTT 2475 0.10592349883933525 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2460 0.10528153824030896 No Hit CTGTAGGACGTGGAATATGGCAAGA 2370 0.10142977464615133 No Hit GTACTGGTTCACTATCGGTCAGTCA 2368 0.10134417989961447 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.279737326841828E-5 0.0 0.0 0.0 0.0 6 4.279737326841828E-5 0.0 0.0 0.0 0.0 7 4.279737326841828E-5 0.0 0.0 0.0 0.0 8 4.279737326841828E-5 0.0 0.0 0.0 0.0 9 4.279737326841828E-5 0.0 0.0 0.0 0.0 10 4.279737326841828E-5 0.0 0.0 4.279737326841828E-5 0.0 11 1.2839211980525484E-4 0.0 0.0 4.279737326841828E-5 0.0 12 1.2839211980525484E-4 0.0 0.0 4.279737326841828E-5 4.279737326841828E-5 13 1.2839211980525484E-4 0.0 0.0 4.279737326841828E-5 8.559474653683656E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGC 80 1.2725468E-7 14.265309 6 GACCGCG 85 2.6798443E-7 13.420418 7 TAGGACC 775 0.0 12.516658 4 CGTGCGC 140 5.2750693E-11 12.21013 10 TACGCTC 55 0.0030446553 12.103899 4 ACGATAC 65 7.945517E-4 11.706374 3 TGGATCG 75 2.0540786E-4 11.412247 5 GAACCGA 75 2.0540786E-4 11.412247 6 GCGTGCG 140 8.381903E-9 10.855075 9 TGGCGAG 1675 0.0 10.823819 18 GGCGAGG 755 0.0 10.686005 19 GTAGGAC 1765 0.0 10.616157 3 TGTAGGA 1790 0.0 10.521249 2 GGTATCA 2140 0.0 10.489465 1 GTCCTAA 765 0.0 10.444146 1 AGGACCT 1285 0.0 10.287305 5 TATGGCG 1810 0.0 10.17428 16 GGGTATA 75 0.0026216707 10.145742 1 GTCCTAC 1805 0.0 10.117639 1 GTACTAG 180 3.8562575E-10 10.040057 1 >>END_MODULE