FastQCFastQC Report
Thu 26 May 2016
SRR1512845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605044
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47540.2961912570621117No Hit
TATCAACGCAGAGTACTTTTTTTTT27150.16915424125444536No Hit
GGTATCAACGCAGAGTACTTTTTTT26750.16666209773688448No Hit
GTACTGGTTCACTATCGGTCAGTCA23410.14585269936525105No Hit
GTCCTAAAGTGTGTATTTCTCATTT17290.10772290354656945No Hit
GTCCTACAGTGGACATTTCTAAATT16140.10055799093358188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1102.750312E-912.95322818
TAGGACC2700.012.3127934
GGACCGT853.949399E-612.2924836
CTCGTCG550.003073010412.08854910
GCCTTAT1209.618816E-911.9072171
ACCCGAA658.03262E-411.6907537
ATAGACC1001.9333274E-611.3981293
GTCCTAG1850.011.3279481
TAGGACA2950.011.2693374
GGTATCA14850.011.1615131
TAGATTG600.005882981211.0815145
GTATATG2000.010.954641
CTACGAG700.001493490410.855363
CTAGGAC1951.8189894E-1210.7161893
AGCACCG803.776539E-410.6857455
GTAGGAC7650.010.5538233
CGAATGG1355.7018042E-810.553493519
TCTAGAC1409.7079464E-810.1769013
GTATCAA30250.010.0768521
CGGTTTC856.6012493E-410.05748713
Produced by FastQC (version 0.11.5)