##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512845_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1605044
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.556152977737682	31.0	30.0	34.0	16.0	34.0
2	28.582215814644332	31.0	29.0	34.0	16.0	34.0
3	28.76755528197358	31.0	30.0	34.0	16.0	34.0
4	32.14865199957135	35.0	33.0	37.0	19.0	37.0
5	32.35737649559763	35.0	33.0	37.0	22.0	37.0
6	32.21530873919967	35.0	33.0	37.0	21.0	37.0
7	32.16275130152195	35.0	33.0	37.0	19.0	37.0
8	32.08898447643803	35.0	33.0	37.0	18.0	37.0
9	33.353375982216065	38.0	33.0	39.0	17.0	39.0
10	33.220015775268465	38.0	33.0	39.0	17.0	39.0
11	33.29236394765502	38.0	33.0	39.0	17.0	39.0
12	33.186812947184	38.0	33.0	39.0	17.0	39.0
13	33.148096874602814	38.0	33.0	39.0	17.0	39.0
14	33.966989690002265	39.0	33.0	41.0	15.0	41.0
15	34.1382211328786	39.0	33.0	41.0	17.0	41.0
16	34.069725814370194	39.0	33.0	41.0	16.0	41.0
17	33.802554322498324	39.0	33.0	41.0	10.0	41.0
18	33.80265712341842	39.0	33.0	41.0	10.0	41.0
19	33.908669170440184	39.0	33.0	41.0	10.0	41.0
20	33.886246732176815	39.0	34.0	41.0	10.0	41.0
21	33.77254579936749	39.0	33.0	41.0	8.0	41.0
22	33.782282604090604	39.0	34.0	41.0	2.0	41.0
23	33.436462177983906	39.0	33.0	41.0	2.0	41.0
24	33.49706238582868	39.0	33.0	41.0	2.0	41.0
25	33.20689962393554	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7229202064985181
1101	2	0.37797453714221874
1101	3	0.8405371228971035
1101	4	0.8910077074542997
1101	5	0.6679317819438069
1101	6	0.6530833104085687
1101	7	0.41677745155499224
1101	8	0.7155046885163472
1101	9	0.7714090546148853
1101	10	0.7208062041322805
1101	11	0.8229485303013249
1101	12	0.6770238732772071
1101	13	1.0768983867578683
1101	14	1.1427530315675298
1101	15	0.9528825188040173
1101	16	0.7836343985070542
1101	17	0.27651272267749505
1101	18	0.719600346580684
1101	19	0.23474138516935739
1101	20	-0.37472102019341946
1101	21	0.6433948643364786
1101	22	0.6539380080843813
1101	23	0.2657535534975608
1101	24	0.7865193841349623
1101	25	0.4182924876714722
1102	1	0.9316878824042973
1102	2	0.9853684681634363
1102	3	0.8760612832132786
1102	4	0.983523098638571
1102	5	0.9602362913864155
1102	6	0.8212538808253953
1102	7	0.8504165769578407
1102	8	0.8806518757101216
1102	9	1.1233555459124531
1102	10	1.0664866837561888
1102	11	0.7377105569601952
1102	12	0.9280251213666375
1102	13	0.9492619417337309
1102	14	1.2271086063557206
1102	15	1.3171165623225818
1102	16	1.0989384941772116
1102	17	1.3594312677424583
1102	18	1.3582862431700775
1102	19	1.142189594198264
1102	20	1.3319307276396941
1102	21	1.3528885043625607
1102	22	1.256076487094738
1102	23	1.2415903592012612
1102	24	0.5702741762092245
1102	25	0.9968513540839155
1103	1	0.637037625626725
1103	2	0.6906303176638033
1103	3	0.8561899228906675
1103	4	1.1762782946169779
1103	5	0.9138380810075475
1103	6	0.6809307336529358
1103	7	0.2651134239673425
1103	8	0.6548171559189129
1103	9	0.6976108187978625
1103	10	1.0848026859561557
1103	11	0.7484383464953979
1103	12	0.7964451038075708
1103	13	0.9008229899909921
1103	14	0.96773751217399
1103	15	0.999451760773816
1103	16	1.0857550917915546
1103	17	0.6114222409260179
1103	18	1.0760429395730284
1103	19	1.037577043171531
1103	20	1.104791704368516
1103	21	0.9131303039274883
1103	22	0.976398827481546
1103	23	0.2625320223669334
1103	24	0.494815120089406
1103	25	0.8736730580611436
1104	1	0.9085612792065199
1104	2	0.875097429066777
1104	3	0.8519396291983874
1104	4	1.1217931586168177
1104	5	0.9177286474552844
1104	6	0.8589398010735962
1104	7	0.9654499553364175
1104	8	0.613501733115136
1104	9	1.0345570371851238
1104	10	0.9546977214334191
1104	11	0.7033269887687936
1104	12	1.0872520813053441
1104	13	1.0578861500020693
1104	14	1.2517878943802003
1104	15	0.9700074963523093
1104	16	0.846033365827239
1104	17	1.2814841720017682
1104	18	1.0909709829722516
1104	19	0.591083523519238
1104	20	0.9252142070441707
1104	21	1.0760905692016394
1104	22	0.8526203149552956
1104	23	1.2107379902705944
1104	24	1.0967299268642634
1104	25	0.5972870328114084
1105	1	0.6598004885367033
1105	2	0.7166361736885669
1105	3	0.8806583729588837
1105	4	1.015308460324924
1105	5	0.7712477075463511
1105	6	0.9169025226126521
1105	7	0.9973611322192255
1105	8	0.9690878255322062
1105	9	0.6471022062927787
1105	10	0.8694538376144294
1105	11	0.889102135678506
1105	12	1.0089193939440833
1105	13	1.0119259810314318
1105	14	1.1274394453004177
1105	15	1.15202833188782
1105	16	1.0744467409725829
1105	17	1.436501026000336
1105	18	1.1545968046291506
1105	19	0.791899092620632
1105	20	1.0199843388616188
1105	21	0.9297339691288187
1105	22	0.9059160644590705
1105	23	1.0070153119529408
1105	24	1.132956510376701
1105	25	1.4045418081110057
1106	1	0.6570220384193384
1106	2	0.6043391277411523
1106	3	0.6247912850195725
1106	4	0.7734008749806662
1106	5	0.6748391922075285
1106	6	0.6969611650642662
1106	7	0.611921516887719
1106	8	0.5921516905733455
1106	9	0.7392910642269754
1106	10	0.5647353132599449
1106	11	0.3472445832292479
1106	12	0.5862375982829704
1106	13	0.6200787438056636
1106	14	0.8531860806694525
1106	15	0.5232106839212207
1106	16	0.7292043332207356
1106	17	0.8989982847500002
1106	18	0.7592055165257747
1106	19	0.7764195971731382
1106	20	0.9120689074497363
1106	21	0.7637353823956587
1106	22	0.7043462011560848
1106	23	0.9991528374364194
1106	24	0.6300526531328998
1106	25	0.9358807566079861
1107	1	0.9393970144779189
1107	2	0.9606675068179698
1107	3	1.0636706436814585
1107	4	0.9677521114166652
1107	5	0.9611586876940308
1107	6	0.9393108987650862
1107	7	0.7460404710174018
1107	8	0.9987249699271317
1107	9	0.9583990927276105
1107	10	1.1184386860406121
1107	11	0.9569148958360216
1107	12	1.0651677047352734
1107	13	0.8929843066528136
1107	14	1.1175911159230907
1107	15	0.8971806414089585
1107	16	1.0391477945742338
1107	17	1.1587141702713524
1107	18	1.202982559903937
1107	19	0.9233419576736637
1107	20	1.2274973201167256
1107	21	1.0185437980976388
1107	22	1.0485521207905535
1107	23	1.2771432852508582
1107	24	1.0512450369219692
1107	25	1.3273503283060037
1108	1	0.7241858835979507
1108	2	0.7662154794452327
1108	3	0.6018887568668383
1108	4	0.7390731846234431
1108	5	0.5939010163554883
1108	6	0.6814342801362301
1108	7	0.6373298247406538
1108	8	0.6480022387126567
1108	9	0.7000040182131002
1108	10	0.8222116351333142
1108	11	0.4175481107107899
1108	12	0.6756700690115878
1108	13	0.5858554609359459
1108	14	0.7113708819223419
1108	15	0.7513708748377681
1108	16	0.4061377361590601
1108	17	0.7102966664339476
1108	18	0.7842856865608212
1108	19	0.6979946586989811
1108	20	0.7098688925343808
1108	21	0.6338524679352204
1108	22	0.723117175874556
1108	23	0.8705601012144797
1108	24	0.8620982941202939
1108	25	1.0127694134802354
1109	1	0.7469205951923072
1109	2	0.7747388873359533
1109	3	0.8375128011257971
1109	4	0.7657537167248378
1109	5	0.7863248753329799
1109	6	0.8728045808044982
1109	7	0.7501186943549527
1109	8	0.607580662010271
1109	9	0.7520119755002028
1109	10	0.7951671353702849
1109	11	0.7231225062836657
1109	12	0.6652421577687733
1109	13	0.8101535616474678
1109	14	0.9796722588029567
1109	15	0.8061614285502188
1109	16	0.49875930028439797
1109	17	0.9419379259185021
1109	18	1.0483390868899747
1109	19	0.9610052372281075
1109	20	0.7375793626247642
1109	21	0.7534692972600041
1109	22	0.8130932641347997
1109	23	1.1000655622631896
1109	24	0.8582101440054899
1109	25	0.5760143546671728
1110	1	0.7436978992242445
1110	2	0.8246337771727354
1110	3	0.684442870881135
1110	4	0.7141922369880831
1110	5	0.7311842613258577
1110	6	0.713726678263285
1110	7	0.7028228839801329
1110	8	0.6616117714877063
1110	9	0.9005237703266076
1110	10	0.719515363549803
1110	11	0.7378469605100975
1110	12	0.8026042674741021
1110	13	0.8342071261358583
1110	14	0.814046608732049
1110	15	0.7871595641731872
1110	16	0.9387947273616035
1110	17	1.0056443484008497
1110	18	0.25358688827677156
1110	19	0.6883579332292911
1110	20	0.872321018611828
1110	21	1.0108239229651659
1110	22	0.9513386424034422
1110	23	1.0292589730917499
1110	24	1.057054398831184
1110	25	1.0535906524834147
1111	1	0.44937790420390655
1111	2	0.4307707574013442
1111	3	0.41853595719480197
1111	4	0.37998498277168835
1111	5	0.4710039610582015
1111	6	0.2807350389366903
1111	7	0.28922991988077484
1111	8	0.3874521277158536
1111	9	0.49917637652780655
1111	10	0.42595613363659623
1111	11	0.12033888753372679
1111	12	-0.19527816084075056
1111	13	0.4131978029298651
1111	14	0.2664136949758742
1111	15	0.3602552602558049
1111	16	0.4850413816499568
1111	17	-0.1978559909146611
1111	18	0.21983251019579342
1111	19	0.3838876221976619
1111	20	0.5074393387647405
1111	21	0.41134602857351155
1111	22	0.24960016281019648
1111	23	0.5532422790723572
1111	24	0.5049221766106484
1111	25	0.5583167785630394
1112	1	0.08031982348906652
1112	2	9.924568332486672E-4
1112	3	0.11849639626833763
1112	4	0.3254341575307649
1112	5	0.1378650916567281
1112	6	-0.042119044542140216
1112	7	-0.17231949699117166
1112	8	0.04571851517029302
1112	9	-0.05843540946340653
1112	10	0.09605071062479453
1112	11	0.057043515107167764
1112	12	0.02465316139090845
1112	13	0.01585313231414176
1112	14	0.010223464942278326
1112	15	-0.12404116185400937
1112	16	0.0063552263917827645
1112	17	0.2475610076242205
1112	18	-0.025755391545409623
1112	19	-0.028480705852089727
1112	20	-0.28118082886808793
1112	21	-0.16866990129287984
1112	22	-0.24353680766680696
1112	23	0.03699750770813637
1112	24	0.043581641430435525
1112	25	0.12697081034892932
1113	1	0.16014111359725547
1113	2	0.20650461258725272
1113	3	0.25141266952356744
1113	4	0.40699085361318055
1113	5	0.1997710843458691
1113	6	-0.18619255401829804
1113	7	-0.05597555957128009
1113	8	0.09899811597416885
1113	9	0.02168126889571198
1113	10	0.08779566396927407
1113	11	0.003313447220612886
1113	12	-0.100953307611789
1113	13	0.01789447611969308
1113	14	0.20770528010307743
1113	15	-0.04131029747414061
1113	16	0.010286000045262256
1113	17	0.015451249789840915
1113	18	-0.20952169724060354
1113	19	-0.5202219668434367
1113	20	-0.22289334225286694
1113	21	0.031698279062744916
1113	22	-0.4924742011819774
1113	23	-4.143822300184006
1113	24	-1.8535905435459092
1113	25	-0.946575589343027
1114	1	-0.46253490195301694
1114	2	-0.7658233727145038
1114	3	-0.5872035701861051
1114	4	-0.5421299561624622
1114	5	-0.43425793718852645
1114	6	-0.9952136576152384
1114	7	-0.8682480834108546
1114	8	-0.7916173929717054
1114	9	-1.141205503401956
1114	10	-0.6733395089848031
1114	11	-0.8516821131499697
1114	12	-1.6705998046125465
1114	13	-0.963695222344505
1114	14	-2.6852756378670897
1114	15	-1.512483500461336
1114	16	-1.6011204570028141
1114	17	-1.4808747474514448
1114	18	-1.3898896798937415
1114	19	-1.553585482742733
1114	20	-1.0692427542512775
1114	21	-0.9530402455123763
1114	22	-1.0838397003550782
1114	23	-5.152325806015355
1114	24	-2.562067323845284
1114	25	-2.511132109056497
1115	1	-1.7977654859595766
1115	2	-1.5086864753078473
1115	3	-1.2806584201990319
1115	4	-1.3395075815905493
1115	5	-1.2669562451446374
1115	6	-1.5424795323243607
1115	7	-2.01388901225047
1115	8	-2.4068338911530773
1115	9	-3.5390556701952605
1115	10	-4.3292640419556285
1115	11	-3.1125652372844748
1115	12	-3.3370289051659867
1115	13	-2.507383646429364
1115	14	-3.2876894216718817
1115	15	-2.90671497434942
1115	16	-3.0334894612079424
1115	17	-2.8085080274341863
1115	18	-5.441895085805026
1115	19	-3.45876392698872
1115	20	-3.654073526068842
1115	21	-3.1956441562323548
1115	22	-2.9016056059959148
1115	23	-3.3910722944730693
1115	24	-3.2329740671168423
1115	25	-3.3431848166915543
1116	1	-1.5323700289493125
1116	2	-1.2748770400094287
1116	3	-1.7436051114235056
1116	4	-1.5600568756559667
1116	5	-1.6030513078269593
1116	6	-3.8652433303461144
1116	7	-2.430355698682181
1116	8	-2.949014999357466
1116	9	-3.8275339821582826
1116	10	-3.092187076575687
1116	11	-2.6184583432276582
1116	12	-2.440268631056604
1116	13	-3.7968120629269286
1116	14	-6.863103604267071
1116	15	-4.846258832004249
1116	16	-3.7946076812481593
1116	17	-4.801586320041807
1116	18	-3.4615971969265473
1116	19	-3.2139142309146393
1116	20	-3.819868533910853
1116	21	-4.681686606011997
1116	22	-4.069832021206189
1116	23	-3.1969210058793927
1116	24	-4.136381847067408
1116	25	-3.942120115878474
1201	1	0.7427319581542555
1201	2	0.7705949519193318
1201	3	1.1680855715610967
1201	4	1.0555367331522092
1201	5	0.5758154290243809
1201	6	0.9889260779093547
1201	7	1.086225430413755
1201	8	1.1354835092182256
1201	9	1.515745835361571
1201	10	1.317186963868231
1201	11	1.58799426018507
1201	12	1.306411304899882
1201	13	1.399082681995651
1201	14	1.549442454424863
1201	15	1.5754825155414807
1201	16	1.531005338008761
1201	17	1.7808532553409222
1201	18	1.6216155064993387
1201	19	1.8046052014668135
1201	20	1.6640684972774622
1201	21	1.8954754109200564
1201	22	1.6831261531149906
1201	23	2.0606786782895767
1201	24	1.8692219604511209
1201	25	1.9396872491256971
1202	1	0.9694391927722386
1202	2	0.9100529190142161
1202	3	0.9583239082750907
1202	4	1.146546987828792
1202	5	0.9770268331262528
1202	6	1.2937040810646394
1202	7	1.2665499740243007
1202	8	1.35008515447403
1202	9	1.5817476383816285
1202	10	1.4626681448463685
1202	11	1.3541650963356204
1202	12	1.5157954608611206
1202	13	1.524096768089855
1202	14	1.9143934348170148
1202	15	1.6781785939728522
1202	16	1.335242435146462
1202	17	1.7576224687734197
1202	18	1.7094225833530672
1202	19	1.7618539055461468
1202	20	1.5713453735452134
1202	21	1.7715036957972288
1202	22	1.7820922338001424
1202	23	1.776500179303774
1202	24	1.9399566911250048
1202	25	2.2050326401556717
1203	1	1.0312236260078365
1203	2	1.0433936194089277
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2211	16	-0.7519319876646122
2211	17	0.01308234655277829
2211	18	-0.3709977277540233
2211	19	-0.33656294307338186
2211	20	-0.4382404199199925
2211	21	-0.18718143100992535
2211	22	-0.2290158268192286
2211	23	-0.08183724527729908
2211	24	-0.42456524657605854
2211	25	-0.03842044830572178
2212	1	0.17370705089405192
2212	2	0.2753865081814766
2212	3	-0.13249074004462713
2212	4	0.26831936872896023
2212	5	0.02253420662550809
2212	6	-0.10840654883503476
2212	7	0.18797423804491586
2212	8	0.04763463092817233
2212	9	0.17980695530503965
2212	10	-0.1579940995481266
2212	11	-0.31802941054448297
2212	12	0.2517721068783345
2212	13	0.23439225627581095
2212	14	0.2479387448329149
2212	15	-0.2311218829746835
2212	16	-0.2600209824293245
2212	17	-1.7041538863082906E-4
2212	18	0.2554699491773107
2212	19	-0.04457118079780287
2212	20	0.14687851317571443
2212	21	0.40010136039894917
2212	22	0.3801871847348508
2212	23	0.30272955269339263
2212	24	0.344756318338419
2212	25	0.5097609993465042
2213	1	-0.39445662201318754
2213	2	-0.20626296859334659
2213	3	-0.5201020293845744
2213	4	-0.2488775348762644
2213	5	-0.32996633586355983
2213	6	-0.19898957117293037
2213	7	-0.29388037235303344
2213	8	0.013191161452745348
2213	9	-0.6324918255338048
2213	10	-0.1961034033464344
2213	11	-0.3940351346740769
2213	12	-0.3958995869591817
2213	13	-0.3938439361910895
2213	14	-0.569111164794414
2213	15	-0.19337871976043886
2213	16	-0.24828613716998404
2213	17	-0.25187427392519623
2213	18	-0.29954998848231185
2213	19	-0.29063663249982596
2213	20	-0.08506102918493497
2213	21	-0.40667968974602076
2213	22	-0.5726534335571856
2213	23	-0.29367866353294403
2213	24	-0.05771157991024012
2213	25	0.15211123914881597
2214	1	-2.74245970693622
2214	2	-1.3912511906105642
2214	3	-1.716879909808302
2214	4	-3.5616703050757295
2214	5	-1.9156153340632756
2214	6	-1.6026165948939557
2214	7	-1.3424675902028511
2214	8	-1.1798010987299925
2214	9	-0.9866709941783967
2214	10	-0.9746504570594112
2214	11	-1.0547746909109108
2214	12	-0.9483579929585986
2214	13	-1.3316864477355566
2214	14	-1.2805848137171196
2214	15	-1.711995417754025
2214	16	-1.6473855789153546
2214	17	-3.5865705094490252
2214	18	-3.319613503861504
2214	19	-2.2790499379737454
2214	20	-2.1468237856807377
2214	21	-1.8916111010695218
2214	22	-3.046752427470512
2214	23	-1.8778261448615474
2214	24	-2.9847253526649062
2214	25	-2.92963714193834
2215	1	-1.6423777292850765
2215	2	-3.3241559055193513
2215	3	-1.8793416270411178
2215	4	-3.541919262447351
2215	5	-2.1582572429487
2215	6	-1.8910182694665565
2215	7	-2.131747778671862
2215	8	-1.323585441794524
2215	9	-3.6578482887567247
2215	10	-2.104011508920223
2215	11	-1.6594127158066456
2215	12	-1.7261301110064124
2215	13	-1.7702522367903484
2215	14	-1.3462239210362128
2215	15	-1.6487637473201033
2215	16	-1.718820585760085
2215	17	-2.007855409643227
2215	18	-1.6224328983411453
2215	19	-1.7879028875773884
2215	20	-1.5713027898512522
2215	21	-2.2571967239404884
2215	22	-2.1625987870260808
2215	23	-2.227044093987651
2215	24	-2.06229101437577
2215	25	-3.2770423503048356
2216	1	-2.9512469718498693
2216	2	-2.7985295685070675
2216	3	-4.402388784529332
2216	4	-4.578861503516581
2216	5	-4.633864459502096
2216	6	-3.6697987249499633
2216	7	-5.433547373545306
2216	8	-3.0440049560212827
2216	9	-3.7796096427123906
2216	10	-2.5704734667794895
2216	11	-3.088936218826163
2216	12	-3.5230784100428174
2216	13	-3.014938831644862
2216	14	-3.87246968519198
2216	15	-3.485425050161666
2216	16	-3.696410650693924
2216	17	-3.3969010905019417
2216	18	-3.056098536415469
2216	19	-3.8587349115063567
2216	20	-4.505697277101124
2216	21	-4.134680395825843
2216	22	-3.72339995318476
2216	23	-3.7773710349035383
2216	24	-4.161453879036998
2216	25	-4.211257370277195
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92910.0
3	1.0
4	817.0
5	4924.0
6	5760.0
7	5211.0
8	4599.0
9	4210.0
10	3829.0
11	3680.0
12	3477.0
13	3402.0
14	3333.0
15	3475.0
16	3561.0
17	3781.0
18	4188.0
19	4974.0
20	6775.0
21	8452.0
22	10571.0
23	12682.0
24	14676.0
25	16557.0
26	18385.0
27	20023.0
28	22649.0
29	26596.0
30	31890.0
31	40126.0
32	49486.0
33	62709.0
34	80640.0
35	105260.0
36	140970.0
37	215787.0
38	564678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73575422661336	17.51075993086139	12.059561726214849	24.6939241163104
2	16.189607999157563	19.62091609969412	39.24357689007639	24.94589901107193
3	19.915533572850112	24.45688137190027	28.273759453871833	27.353825601377785
4	13.164734112585997	15.692164666194355	36.04433004272617	35.09877117849348
5	14.219637547325473	37.80186734660814	33.55316778453233	14.425327321534057
6	33.403169306092316	34.42595453927588	17.705040286626787	14.465835868005023
7	29.643192511681793	31.07075916991005	20.61956205908798	18.66648625932018
8	27.451792403288554	33.97545681679554	19.949568972506796	18.623181807409107
9	28.320120320105357	14.265689439940338	18.934703146804747	38.47948709314956
10	17.441338385287143	27.44705051870661	31.766196642063747	23.345414453942496
11	35.78885996227415	21.985065552558964	22.900088858403358	19.32598562676353
12	24.875600171874133	23.94900152728502	29.377816886695907	21.797581414144943
13	29.997611804048212	20.847204719524157	24.855819684346592	24.299363792081042
14	22.916279528025512	21.033257963415867	26.207093086628845	29.84336942192978
15	25.09571591765064	28.445960102363028	22.69489880925657	23.76342517072976
16	24.0686074601197	26.79478497500115	25.38383736345417	23.752770201424976
17	23.233334934039473	26.9335230897703	26.00010352618932	23.833038450000902
18	23.328028059741705	25.881294919161103	27.440072444425596	23.350604576671596
19	24.63876572312336	25.829763085193292	26.3775545203395	23.153916671343847
20	25.494873224399484	25.314085942245356	26.291983621258442	22.89905721209672
21	25.374419563161066	25.251894155506875	25.728652052160285	23.64503422917177
22	25.46224488204936	25.77937851786021	25.570824952835338	23.187551647255095
23	24.509348431679836	25.71312933769288	26.083125302847275	23.694396927780005
24	24.64471792889058	25.353380082752086	26.260160827146674	23.741741161210662
25	24.346776833736065	25.530470028840906	26.383350222152934	23.73940291527009
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	257.0
1	257.0
2	532.0
3	807.0
4	807.0
5	807.0
6	1374.0
7	1941.0
8	1941.0
9	1941.0
10	2180.0
11	2419.0
12	2419.0
13	2419.0
14	3268.0
15	4117.0
16	4117.0
17	4117.0
18	8033.5
19	11950.0
20	11950.0
21	11950.0
22	19114.0
23	26278.0
24	26278.0
25	26278.0
26	40532.5
27	54787.0
28	54787.0
29	54787.0
30	71016.5
31	87246.0
32	87246.0
33	87246.0
34	100706.5
35	114167.0
36	114167.0
37	114167.0
38	127944.5
39	141722.0
40	141722.0
41	141722.0
42	157373.0
43	173024.0
44	173024.0
45	173024.0
46	193970.0
47	214916.0
48	214916.0
49	214916.0
50	216968.0
51	219020.0
52	219020.0
53	219020.0
54	203366.5
55	187713.0
56	187713.0
57	187713.0
58	167797.0
59	147881.0
60	147881.0
61	147881.0
62	125730.0
63	103579.0
64	103579.0
65	103579.0
66	81673.0
67	59767.0
68	59767.0
69	59767.0
70	45491.5
71	31216.0
72	31216.0
73	31216.0
74	22534.5
75	13853.0
76	13853.0
77	13853.0
78	9747.5
79	5642.0
80	5642.0
81	5642.0
82	3774.0
83	1906.0
84	1906.0
85	1906.0
86	1279.0
87	652.0
88	652.0
89	652.0
90	402.0
91	152.0
92	152.0
93	152.0
94	88.0
95	24.0
96	24.0
97	24.0
98	16.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33388492776522016
2	0.01090312788932889
3	0.009096323839097246
4	0.010778520713450847
5	0.012460717587804447
6	0.0801224140895826
7	0.07887634233080215
8	0.10379777750641103
9	0.0823653432553874
10	0.08971716663219202
11	0.08529361188852144
12	0.09625904336578935
13	0.08242764684332642
14	0.08710041593875309
15	0.0834868078382898
16	0.08535591547646046
17	0.09875118688335024
18	0.10018416940594775
19	0.09432763213967967
20	0.0935799890844114
21	0.0806831463810338
22	0.100869508873277
23	0.09426532855174063
24	0.09090093480303343
25	0.08840879128547255
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1605044.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.444829555009264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.39744707844359	39.7615887843524
2	13.791625012285957	14.741820961364512
3	4.939014600795008	7.918943805275738
4	2.3315051208410935	4.984275751599341
5	1.3062505152493253	3.4906168071821613
6	0.7811753934058276	2.5049871451885064
7	0.5240601910281955	1.9605815310247254
8	0.3461355422482945	1.4799324046712647
9	0.24572858645101575	1.1819630177701106
>10	1.2150162731012797	11.333017142471737
>50	0.07290088710086236	2.710418432752833
>100	0.04230614489641719	4.242242366998259
>500	0.004831393453079332	1.8054756763184985
>1k	0.002003260700057284	1.8841361730298771
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4754	0.2961912570621117	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2715	0.16915424125444536	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2675	0.16666209773688448	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2341	0.14585269936525105	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1729	0.10772290354656945	No Hit
GTCCTACAGTGGACATTTCTAAATT	1614	0.10055799093358188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	6.230358793902223E-5	0.0
7	0.0	0.0	0.0	6.230358793902223E-5	0.0
8	0.0	0.0	0.0	6.230358793902223E-5	6.230358793902223E-5
9	0.0	0.0	0.0	1.2460717587804446E-4	6.230358793902223E-5
10	0.0	0.0	0.0	1.2460717587804446E-4	6.230358793902223E-5
11	0.0	0.0	0.0	1.2460717587804446E-4	6.230358793902223E-5
12	0.0	0.0	0.0	1.869107638170667E-4	6.230358793902223E-5
13	0.0	0.0	0.0	1.869107638170667E-4	6.230358793902223E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	110	2.750312E-9	12.953228	18
TAGGACC	270	0.0	12.312793	4
GGACCGT	85	3.949399E-6	12.292483	6
CTCGTCG	55	0.0030730104	12.088549	10
GCCTTAT	120	9.618816E-9	11.907217	1
ACCCGAA	65	8.03262E-4	11.690753	7
ATAGACC	100	1.9333274E-6	11.398129	3
GTCCTAG	185	0.0	11.327948	1
TAGGACA	295	0.0	11.269337	4
GGTATCA	1485	0.0	11.161513	1
TAGATTG	60	0.0058829812	11.081514	5
GTATATG	200	0.0	10.95464	1
CTACGAG	70	0.0014934904	10.85536	3
CTAGGAC	195	1.8189894E-12	10.716189	3
AGCACCG	80	3.776539E-4	10.685745	5
GTAGGAC	765	0.0	10.553823	3
CGAATGG	135	5.7018042E-8	10.5534935	19
TCTAGAC	140	9.7079464E-8	10.176901	3
GTATCAA	3025	0.0	10.076852	1
CGGTTTC	85	6.6012493E-4	10.057487	13
>>END_MODULE