FastQCFastQC Report
Thu 26 May 2016
SRR1512845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605044
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60790.37874351108131615No Hit
TATCAACGCAGAGTACTTTTTTTTT45980.2864718973436242No Hit
GGTATCAACGCAGAGTACTTTTTTT36090.2248536488719312No Hit
GTACTGGTTCACTATCGGTCAGTCA21900.1364448575864587No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21670.13501187506386117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAT604.049067E-412.6825681
ACGACCA550.00304123912.1053335
TAGACTA802.8317307E-511.8891655
GGTATCA15600.011.5240651
TAACACT1553.0559022E-1011.0454194
ATCGCGC2000.010.9250738
TACGACC700.001476685610.8700954
CCCGTTT1158.907027E-710.73050112
TCGCGCG2050.010.6566139
TATCGCG2150.010.611677
CGCGCCT2150.010.5961312
GTATAGA1451.41481E-810.4959191
TAGAAAT2450.010.4818774
GTCCTAA3700.010.2831641
TTAGTAC750.002620232510.1460563
TCTACAC2250.010.1460543
TCTAACC856.5175677E-410.0714523
GCCCCGT2002.5465852E-119.96698614
TAGGACA3350.09.9372134
CCTAATA1252.5283152E-69.8924042
Produced by FastQC (version 0.11.5)