FastQCFastQC Report
Thu 26 May 2016
SRR1512844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064288
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52100.2523872637926491No Hit
TATCAACGCAGAGTACTTTTTTTTT30590.14818668712892774No Hit
GGTATCAACGCAGAGTACTTTTTTT29680.14377838751182007No Hit
GTACTGGTTCACTATCGGTCAGTCA26910.130359717248756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4700.012.9340894
GGTATCA16700.011.9787611
TAGGACT2750.011.7435654
TCTATAC1701.0913936E-1111.1746733
TTAGTAC951.3631086E-510.9982313
GTACCGA700.001493763210.8553966
GTATCAA36200.010.7101341
CGCCCTA3050.010.58871716
ACCCTAC1355.466063E-810.5844081
GTCCTAA5150.010.54330351
GTCCTAC10100.010.4691321
GTCTTAC1658.8584784E-1010.3919641
CGTGCGC1303.8510007E-710.22912410
TGGCGAG10000.010.1636118
CTAGGAC3500.010.0412423
CGAAATC1054.1121428E-59.95102113
TCCTACA11350.09.8750642
TTAACTG5100.09.8709615
ATTTCCG1356.45623E-79.85050514
TAGAAAT2900.09.8260054
Produced by FastQC (version 0.11.5)