##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512844_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2064288
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.75858940225395	31.0	30.0	34.0	20.0	34.0
2	28.788630753073214	31.0	30.0	34.0	16.0	34.0
3	28.97727061340278	31.0	30.0	34.0	22.0	34.0
4	32.38870787409509	35.0	33.0	37.0	22.0	37.0
5	32.59636930505821	35.0	33.0	37.0	25.0	37.0
6	32.463010490784235	35.0	33.0	37.0	23.0	37.0
7	32.41857579950084	36.0	33.0	37.0	23.0	37.0
8	32.346343630346155	36.0	33.0	37.0	22.0	37.0
9	33.64071728363484	38.0	34.0	39.0	21.0	39.0
10	33.50443736532887	38.0	33.0	39.0	18.0	39.0
11	33.576367251081244	38.0	34.0	39.0	19.0	39.0
12	33.47674452401991	38.0	34.0	39.0	18.0	39.0
13	33.43877937574602	38.0	34.0	39.0	18.0	39.0
14	34.26817333627866	39.0	33.0	41.0	17.0	41.0
15	34.446233761955696	39.0	34.0	41.0	17.0	41.0
16	34.382209265373824	39.0	34.0	41.0	17.0	41.0
17	34.10876050241052	39.0	33.0	41.0	17.0	41.0
18	34.1087784262661	39.0	33.0	41.0	17.0	41.0
19	34.22073857911299	39.0	34.0	41.0	18.0	41.0
20	34.19417203413477	39.0	34.0	41.0	16.0	41.0
21	34.09028633601513	39.0	34.0	41.0	11.0	41.0
22	34.085051601326946	39.0	34.0	41.0	10.0	41.0
23	33.744872324016804	39.0	33.0	41.0	2.0	41.0
24	33.80526845091383	39.0	34.0	41.0	2.0	41.0
25	33.51073202963928	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6431169675894317
1101	2	0.14503078289785165
1101	3	0.542758165152204
1101	4	0.6901358813315994
1101	5	0.4445058586549422
1101	6	0.5053347156623502
1101	7	0.10484463257066068
1101	8	0.43314812247276535
1101	9	0.5768665837091618
1101	10	0.6263115071384959
1101	11	0.6863905672823023
1101	12	0.5937062722981281
1101	13	1.0224976740541862
1101	14	1.0745518438476083
1101	15	0.8803739469507761
1101	16	0.717021870602025
1101	17	0.21561020106928908
1101	18	0.706068424741467
1101	19	0.005946519632892944
1101	20	-0.4976555841545931
1101	21	0.46988393283964314
1101	22	0.5737831981247226
1101	23	0.15256589088322414
1101	24	0.7495133961966189
1101	25	0.4611808545247271
1102	1	0.6688717844448249
1102	2	0.7252604242146745
1102	3	0.6090795113564269
1102	4	0.829804128176356
1102	5	0.6535715638159303
1102	6	0.463493292691723
1102	7	0.41243979728186275
1102	8	0.4819671609911609
1102	9	0.7071977792488795
1102	10	0.7803505891669431
1102	11	0.5603772815909949
1102	12	0.6327790740192754
1102	13	0.6425944183417371
1102	14	0.7094958034874708
1102	15	0.6478994217563852
1102	16	0.7489748447587559
1102	17	0.8268950154334647
1102	18	0.6866034307609041
1102	19	0.46227098286777135
1102	20	0.6842785153184039
1102	21	0.8330109845338143
1102	22	0.5847758460242218
1102	23	0.4742552894086316
1102	24	-0.1285715916301129
1102	25	0.3112520808817223
1103	1	0.683051368278722
1103	2	0.7604411522798173
1103	3	0.9263716444859327
1103	4	1.1188987121156373
1103	5	1.0270773808007831
1103	6	0.6027672524586691
1103	7	0.5493431557354924
1103	8	0.7438264215329511
1103	9	0.7919094000803071
1103	10	0.8801828447482976
1103	11	0.7180618610215035
1103	12	0.8197653493505825
1103	13	1.051336215046767
1103	14	0.9557484904808433
1103	15	0.9558274334787953
1103	16	1.0453161609221198
1103	17	0.7255691923857057
1103	18	1.1093480257012729
1103	19	0.9834901790852086
1103	20	1.2787073363150583
1103	21	1.046274986396412
1103	22	0.9399793799240115
1103	23	0.2907421862964341
1103	24	0.4634199518135489
1103	25	0.858072786404982
1104	1	0.8831247819903645
1104	2	0.8844655347054697
1104	3	0.8156595766577368
1104	4	0.9684440478699514
1104	5	0.7782820798165773
1104	6	0.7262241636176014
1104	7	0.810621671190674
1104	8	0.5790058146366519
1104	9	0.8476838287139259
1104	10	0.8989623566903333
1104	11	0.6767323827250209
1104	12	0.9301043894033469
1104	13	0.954266717790361
1104	14	1.1264073040525915
1104	15	0.9901068694408082
1104	16	0.9606710548956272
1104	17	1.202351265061047
1104	18	0.9805703986019836
1104	19	0.4461262234490846
1104	20	0.6804267242726851
1104	21	0.7132250042308144
1104	22	0.5509871498782388
1104	23	1.0614256441149337
1104	24	0.942598590980154
1104	25	0.47259639840957135
1105	1	0.6449883723628957
1105	2	0.7462463682941944
1105	3	0.8234575494765899
1105	4	0.8913708192920424
1105	5	0.6794744639479617
1105	6	0.8348782375491766
1105	7	0.8631829370692685
1105	8	0.9552344826003321
1105	9	0.5732523642648815
1105	10	0.6174925909727378
1105	11	0.7640018381210396
1105	12	0.8164569456471398
1105	13	0.8215147924022119
1105	14	0.9819974177171531
1105	15	0.8518006349249703
1105	16	0.726943508483231
1105	17	1.0755101510718816
1105	18	0.9826786438018473
1105	19	0.678220798675973
1105	20	0.886020330948746
1105	21	0.7814730246939732
1105	22	0.6905081294103113
1105	23	0.756188623879126
1105	24	0.9646620788723084
1105	25	1.1578584804183762
1106	1	0.7831383926238757
1106	2	0.8121270533729756
1106	3	0.6001504148788754
1106	4	0.7870467786826367
1106	5	0.7403466074035094
1106	6	0.7836524092108164
1106	7	0.8204775535823927
1106	8	0.8052622389719986
1106	9	0.9937342632847788
1106	10	0.8452458200420523
1106	11	0.623518877254007
1106	12	0.9144229813736118
1106	13	0.9021258737139348
1106	14	1.2447632015672383
1106	15	0.9597838514941586
1106	16	1.0103995983717198
1106	17	1.3579665626375785
1106	18	1.1048228801190518
1106	19	1.0678339818203995
1106	20	1.1122022604967512
1106	21	1.1100064771727958
1106	22	1.0806648260351182
1106	23	1.406308530623967
1106	24	1.0774726984366865
1106	25	1.1763014377563223
1107	1	0.889609671681594
1107	2	0.8942764897770168
1107	3	0.8862743829628812
1107	4	1.0920992811868686
1107	5	0.8970326170980556
1107	6	1.0108872197527674
1107	7	0.7616966248867811
1107	8	0.9439805331011613
1107	9	0.9730458587391695
1107	10	1.1160271977722402
1107	11	0.9773771204925836
1107	12	1.1152246232282437
1107	13	0.9087396348966266
1107	14	1.2045573364848536
1107	15	0.9480824710006814
1107	16	0.8794481631881297
1107	17	1.246019940183551
1107	18	1.1380692849640184
1107	19	0.9703347589956266
1107	20	1.2042851476739358
1107	21	1.0360237131922787
1107	22	0.9219762948387284
1107	23	1.244945365275619
1107	24	1.0326196133388805
1107	25	1.3380955035917026
1108	1	0.6837136488044706
1108	2	0.745815646318956
1108	3	0.6814411170586929
1108	4	0.9002174741225986
1108	5	0.7067080780901804
1108	6	0.8106752206788244
1108	7	0.746222257397477
1108	8	0.7760335411544546
1108	9	0.8385084664014215
1108	10	0.9995360540036344
1108	11	0.704496754524925
1108	12	0.9037289895530662
1108	13	0.7875045170613149
1108	14	0.8302588412364216
1108	15	0.8554360220311992
1108	16	0.6488993083559436
1108	17	0.9027493458727278
1108	18	0.8952301911565002
1108	19	0.9486033350347611
1108	20	0.9417815978631268
1108	21	0.8728732578269245
1108	22	0.8453083900532903
1108	23	1.2320491385001233
1108	24	1.0344178773030421
1108	25	1.0801237446667784
1109	1	0.6935571177059607
1109	2	0.6523044492528278
1109	3	0.767691257311661
1109	4	0.90497122876274
1109	5	0.7293175500979956
1109	6	0.8118993642986041
1109	7	0.7026089265715072
1109	8	0.6579784217616051
1109	9	0.823829472951985
1109	10	0.9077195475267459
1109	11	0.8575667844384682
1109	12	0.664249534566153
1109	13	0.8309441330831504
1109	14	0.8730436504965482
1109	15	0.773502944410744
1109	16	0.4650790756132608
1109	17	0.946388761669283
1109	18	0.9191788766543567
1109	19	0.7972087557321501
1109	20	0.6802540527354353
1109	21	0.6373677833593447
1109	22	0.7334035887259489
1109	23	0.9650026795950097
1109	24	0.6512942242749631
1109	25	0.33212898053087514
1110	1	0.5760830104440728
1110	2	0.589596368897638
1110	3	0.485991959550681
1110	4	0.5174150075507598
1110	5	0.3783342456618257
1110	6	0.5242010297362825
1110	7	0.3821179409331421
1110	8	0.4280456325066524
1110	9	0.5737769956057619
1110	10	0.4491336575561178
1110	11	0.47904253773677397
1110	12	0.4404153174577061
1110	13	0.49106498091474293
1110	14	0.5201713422411842
1110	15	0.4270651175111979
1110	16	0.5364086149217684
1110	17	0.49806503804298785
1110	18	-0.1440951642024615
1110	19	0.3191661444883067
1110	20	0.45191852577075053
1110	21	0.5674003969913173
1110	22	0.521874902856247
1110	23	0.5623948535517229
1110	24	0.548015374650646
1110	25	0.6673197650391955
1111	1	0.3647492105870427
1111	2	0.26341066998906726
1111	3	0.34282053576507465
1111	4	0.3082195708268358
1111	5	0.4014400811415655
1111	6	0.3495060784863355
1111	7	0.21594434031592158
1111	8	0.48528672709893783
1111	9	0.4660137878261281
1111	10	0.5686614045371954
1111	11	0.1584265184225515
1111	12	-0.17566210299862917
1111	13	0.24889120884827065
1111	14	0.3196135582837414
1111	15	0.2438901535496143
1111	16	0.4181185844805597
1111	17	-0.12104547391236764
1111	18	0.3036003498171027
1111	19	0.2250959213137449
1111	20	0.508494985501514
1111	21	0.4371922224488003
1111	22	-0.022937735033963236
1111	23	0.40181128764400853
1111	24	0.3577764683677529
1111	25	0.4402146067481354
1112	1	0.36938554837842474
1112	2	0.38053803766540284
1112	3	0.3240124108663487
1112	4	0.49046843654132033
1112	5	0.34256664801811354
1112	6	0.17581345466981446
1112	7	0.11371161357456572
1112	8	0.28303124954158676
1112	9	0.17319315323388906
1112	10	0.3825666019954639
1112	11	0.2204342471894165
1112	12	0.20942475520411108
1112	13	0.12738476121638342
1112	14	0.2406268123521258
1112	15	0.01300870591772707
1112	16	0.2672945998397154
1112	17	0.5559711562468976
1112	18	0.38291515600474213
1112	19	0.48345928765324686
1112	20	0.158896054536676
1112	21	0.30180189946678837
1112	22	0.08516209800615115
1112	23	0.3349187344309712
1112	24	0.48978048249423267
1112	25	0.44512086181191535
1113	1	-0.12809223425560745
1113	2	0.09599018224701084
1113	3	0.10081782271640449
1113	4	-0.0013448097362100953
1113	5	0.05628484945953005
1113	6	-0.3409475001491771
1113	7	-0.15774052060078247
1113	8	-0.136206506749744
1113	9	-0.14962446813284203
1113	10	-0.12250793656121317
1113	11	-0.17343453284007637
1113	12	-0.3560978917917552
1113	13	-0.24892958663814113
1113	14	0.1198759114672967
1113	15	-0.258259646265806
1113	16	-0.18325076253600514
1113	17	-0.22400835085314696
1113	18	-0.25288765725342444
1113	19	-0.7911169545569265
1113	20	-0.4950049180026852
1113	21	-0.1961083004523374
1113	22	-0.6224000571006485
1113	23	-4.501092910174421
1113	24	-1.991236082785722
1113	25	-1.0448932163718112
1114	1	-0.6573761043466533
1114	2	-0.7109503681272358
1114	3	-0.6622863590296753
1114	4	-0.6704075428604455
1114	5	-0.5773499334385477
1114	6	-1.3536419378916626
1114	7	-1.083585868931717
1114	8	-1.0063627225177463
1114	9	-1.243984837637349
1114	10	-0.8563850993301116
1114	11	-1.123681488532256
1114	12	-2.0412855420658857
1114	13	-1.172757413733109
1114	14	-2.9272508887024244
1114	15	-1.7533510378978718
1114	16	-1.9843550607575366
1114	17	-1.6432991817391098
1114	18	-1.5894101994133791
1114	19	-1.779676014544556
1114	20	-1.2973017030028657
1114	21	-1.229151277121197
1114	22	-1.3516376143330788
1114	23	-5.58446021551482
1114	24	-2.860108295347878
1114	25	-2.79431014580155
1115	1	-1.476037647160421
1115	2	-1.3284560030183066
1115	3	-1.1101584649084053
1115	4	-1.048084691311466
1115	5	-1.0241298727861903
1115	6	-1.2110850958154593
1115	7	-1.6640748810441828
1115	8	-2.01709592458036
1115	9	-3.357761621125885
1115	10	-3.9957268624258084
1115	11	-2.7016464535745257
1115	12	-3.039300876011957
1115	13	-2.219054551052391
1115	14	-2.846406757411966
1115	15	-2.6253672865509223
1115	16	-2.74446675424187
1115	17	-2.5709813118619778
1115	18	-5.63257208454203
1115	19	-3.294131265726854
1115	20	-3.61241105370096
1115	21	-3.3019046931676215
1115	22	-2.6953615680919576
1115	23	-3.283708483233468
1115	24	-3.100776463585138
1115	25	-3.1637635343587505
1116	1	-0.8805501915769902
1116	2	-0.6516966824570822
1116	3	-1.116107236932553
1116	4	-0.7496935708383745
1116	5	-0.7772553136228417
1116	6	-3.3623696235985214
1116	7	-1.8072405241028804
1116	8	-2.249017096432894
1116	9	-2.9371993975655784
1116	10	-2.3557993271287074
1116	11	-1.8263911064209957
1116	12	-1.6753736003793485
1116	13	-3.038058829274572
1116	14	-6.538108437865677
1116	15	-4.145658152394681
1116	16	-3.0195594365719103
1116	17	-4.099442662425272
1116	18	-2.560160854219209
1116	19	-2.182597876160621
1116	20	-3.096612092275702
1116	21	-3.6505587863580473
1116	22	-3.413461015431043
1116	23	-2.3491206319529745
1116	24	-3.452570250030238
1116	25	-3.4691986783664284
1201	1	0.40212329932855084
1201	2	0.4495656136445234
1201	3	0.8396603542167114
1201	4	0.7006488059793909
1201	5	0.23883127777799018
1201	6	0.6340171291534134
1201	7	0.6587089220689251
1201	8	0.7153722733317167
1201	9	1.1817312607892276
1201	10	1.0403740506023595
1201	11	1.2004210904311918
1201	12	1.0328571652507534
1201	13	1.0782099313442188
1201	14	1.0482491331857702
1201	15	1.129673425291628
1201	16	1.0727286263321503
1201	17	1.451432093939701
1201	18	1.1852369845010813
1201	19	1.2694554763578694
1201	20	1.209735916120799
1201	21	1.4651853534165937
1201	22	1.2115963466056456
1201	23	1.493078141744462
1201	24	1.3916084891142617
1201	25	1.4936267677002206
1202	1	0.7175291864596822
1202	2	0.7277670709522042
1202	3	0.7031063203204759
1202	4	0.7914125253061215
1202	5	0.6715408164498058
1202	6	0.9517732632045224
1202	7	0.9365498067487223
1202	8	0.8799357335119353
1202	9	1.098641956425375
1202	10	1.08343517569336
1202	11	0.8444128382166625
1202	12	0.9840205540391906
1202	13	1.0134987889674179
1202	14	1.3818302096275659
1202	15	1.1799620640817778
1202	16	0.9037670233017323
1202	17	1.1847029092795367
1202	18	1.1593025573236986
1202	19	1.1938176382357
1202	20	0.9794014789855083
1202	21	1.1182017562589337
1202	22	1.180501374264935
1202	23	1.2834470179825317
1202	24	1.3986219881022066
1202	25	1.615642758172747
1203	1	0.951974097915496
1203	2	0.9782977272566811
1203	3	0.9361999558400846
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2211	17	0.017874547863513612
2211	18	-0.2160983926722011
2211	19	-0.1988401053925628
2211	20	-0.3601571021980092
2211	21	-0.07264877382119295
2211	22	-0.12405637685937165
2211	23	0.10691432248003707
2211	24	-0.14567001063720397
2211	25	0.23081013774842774
2212	1	0.07105860977373624
2212	2	0.24522602937800997
2212	3	-0.038617319926540716
2212	4	0.09783850610571676
2212	5	-0.11655336387171644
2212	6	-0.12748366322882276
2212	7	0.03492908660061289
2212	8	0.1648098590818421
2212	9	0.16773689938892034
2212	10	-0.2797980106680953
2212	11	-0.2733202632570695
2212	12	0.092441704131069
2212	13	0.13833377764328247
2212	14	0.19974201802848768
2212	15	-0.06322218383808575
2212	16	-0.2537460784722825
2212	17	-0.06001913446247187
2212	18	0.2077469755040724
2212	19	0.02521918253766131
2212	20	0.24669845779129673
2212	21	0.22622252891807904
2212	22	0.25374946313640834
2212	23	0.21383995763448382
2212	24	0.06738742813817566
2212	25	0.1828404318258734
2213	1	-0.3250524851435408
2213	2	-0.21988023755999464
2213	3	-0.46277512749927396
2213	4	-0.16779575873970742
2213	5	-0.21158733001756502
2213	6	-0.03387041857975248
2213	7	-0.2584004739949748
2213	8	-0.07598407272966057
2213	9	-0.6089043374144865
2213	10	-0.27970500264677156
2213	11	-0.45139879040889497
2213	12	-0.4291186099407227
2213	13	-0.498119536932478
2213	14	-0.5553984490136941
2213	15	-0.5152776994630557
2213	16	-0.3965101311213459
2213	17	-0.4525794324256509
2213	18	-0.42553697088956
2213	19	-0.3170379668067582
2213	20	-0.19639885122775524
2213	21	-0.39001573193547756
2213	22	-0.6281103099163516
2213	23	-0.38053796636511095
2213	24	-0.11264599338338144
2213	25	-0.004468805845867507
2214	1	-2.7980926207738506
2214	2	-1.496638914846372
2214	3	-1.6308418866218766
2214	4	-3.5435042871635467
2214	5	-2.0502782303178364
2214	6	-1.6493987215468273
2214	7	-1.5127478644474124
2214	8	-1.2536887269105357
2214	9	-1.0837424335008023
2214	10	-1.1114728407046925
2214	11	-1.1486297245531247
2214	12	-1.1343620383594697
2214	13	-1.458644722692565
2214	14	-1.4625104521690702
2214	15	-1.8220641453381603
2214	16	-1.6582720210394086
2214	17	-3.815613174600827
2214	18	-3.541326896585524
2214	19	-2.3992550961772103
2214	20	-2.2645917300002125
2214	21	-2.2004179788691616
2214	22	-3.140885292103736
2214	23	-2.0346910085046623
2214	24	-3.122826218840146
2214	25	-3.0649728391300037
2215	1	-1.8125013814900335
2215	2	-3.453193330292848
2215	3	-2.077788090754087
2215	4	-3.647158457939664
2215	5	-2.361668678690492
2215	6	-2.046115981871033
2215	7	-2.249551391620365
2215	8	-1.6603085774529838
2215	9	-4.008696755378256
2215	10	-2.425716986318328
2215	11	-1.9629714696154608
2215	12	-1.915071092427194
2215	13	-1.826667745098554
2215	14	-1.5504329352887112
2215	15	-1.71085095653104
2215	16	-1.797834886148273
2215	17	-2.218664915743382
2215	18	-1.865307278701195
2215	19	-1.9715683848755887
2215	20	-1.769125955400412
2215	21	-2.389635254998943
2215	22	-2.21264301470951
2215	23	-2.176642886839641
2215	24	-2.0885921255745856
2215	25	-3.0753468307340803
2216	1	-2.8433292265626626
2216	2	-2.907035000032039
2216	3	-4.146628694975792
2216	4	-4.673862919469972
2216	5	-4.481289647623612
2216	6	-3.3908472515957904
2216	7	-5.0843896979324015
2216	8	-2.7863685982221185
2216	9	-3.49345337201634
2216	10	-2.5418383786396106
2216	11	-2.785486417867393
2216	12	-3.197669726084243
2216	13	-2.870750289441556
2216	14	-3.757149247177516
2216	15	-3.273385557661051
2216	16	-3.4082388469417424
2216	17	-3.2373872982630303
2216	18	-2.6464725376444953
2216	19	-3.442399702745952
2216	20	-4.119949279070795
2216	21	-3.761955253014488
2216	22	-3.1866418510967627
2216	23	-3.4173528710203698
2216	24	-3.712107870991453
2216	25	-3.955207197204441
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107494.0
3	1.0
4	1056.0
5	5713.0
6	6841.0
7	6342.0
8	5464.0
9	5233.0
10	4612.0
11	4358.0
12	4361.0
13	4149.0
14	4050.0
15	4246.0
16	4267.0
17	4690.0
18	5255.0
19	6371.0
20	8089.0
21	10409.0
22	13084.0
23	15736.0
24	18579.0
25	20507.0
26	23455.0
27	25840.0
28	29284.0
29	34148.0
30	41577.0
31	51458.0
32	64232.0
33	81794.0
34	104790.0
35	137162.0
36	184547.0
37	281912.0
38	733182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.78445129553096	17.537749967313417	11.956436681103849	24.72136205605178
2	16.188098089601525	19.6383168804189	39.28777678298044	24.885808246999137
3	19.68731983585987	24.684291867118198	28.14673778759647	27.48165050942546
4	12.999754852325172	15.581605591304912	36.28902620173231	35.1296133546376
5	14.165360568013623	37.905558772016946	33.618083026692865	14.310997633276568
6	33.27601227740418	34.656371824414315	17.78686748267157	14.280748415509944
7	29.996911813114757	30.935827282604006	20.689106844963806	18.378154059317428
8	27.524972468335555	34.030979972077965	19.959256531102415	18.48479102848406
9	28.33018036406371	14.350874573234055	18.950347279121655	38.368597783580576
10	17.2322973632777	27.57879353990672	31.973379194713853	23.21552990210173
11	35.78283140489056	22.017992334712698	22.86015577841938	19.33902048197736
12	24.536132362653188	24.144585697564022	29.582453666573894	21.7368282732089
13	30.003277420030468	20.78175194584694	24.97597689903704	24.238993735085558
14	22.869775133974652	20.8941308120127	26.22160205224975	30.014492001762893
15	24.89837831967388	28.52631109421726	22.590800134978416	23.984510451130443
16	24.221651081506824	26.826450311969857	25.320684845232016	23.6312137612913
17	23.35382011875062	27.09604059535326	25.894181510494324	23.655957775401795
18	23.334000885435938	26.111437882983772	27.3630738301311	23.191487401449187
19	24.640399310109743	25.958163402359535	26.409540152359657	22.99189713517107
20	25.21670152089872	25.435427383353076	26.474751005988654	22.873120089759546
21	25.14899688600993	25.29803013341724	25.81921016257423	23.733762817998603
22	25.196798466926122	25.90155857985067	25.726992981493137	23.174649971730073
23	24.558187121033964	25.708668003326295	26.132552349134848	23.600592526504894
24	24.536692697146215	25.533660387092272	26.30793220115455	23.621714714606966
25	24.267790744299006	25.769276084113823	26.34503617227906	23.61789699930812
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	264.0
1	264.0
2	603.5
3	943.0
4	943.0
5	943.0
6	1647.0
7	2351.0
8	2351.0
9	2351.0
10	2553.5
11	2756.0
12	2756.0
13	2756.0
14	3807.5
15	4859.0
16	4859.0
17	4859.0
18	9790.5
19	14722.0
20	14722.0
21	14722.0
22	23589.5
23	32457.0
24	32457.0
25	32457.0
26	50172.5
27	67888.0
28	67888.0
29	67888.0
30	88205.5
31	108523.0
32	108523.0
33	108523.0
34	129963.0
35	151403.0
36	151403.0
37	151403.0
38	171154.0
39	190905.0
40	190905.0
41	190905.0
42	211153.5
43	231402.0
44	231402.0
45	231402.0
46	254509.5
47	277617.0
48	277617.0
49	277617.0
50	280447.0
51	283277.0
52	283277.0
53	283277.0
54	262014.0
55	240751.0
56	240751.0
57	240751.0
58	213097.5
59	185444.0
60	185444.0
61	185444.0
62	158094.5
63	130745.0
64	130745.0
65	130745.0
66	102309.5
67	73874.0
68	73874.0
69	73874.0
70	55998.5
71	38123.0
72	38123.0
73	38123.0
74	27361.0
75	16599.0
76	16599.0
77	16599.0
78	11472.5
79	6346.0
80	6346.0
81	6346.0
82	4240.0
83	2134.0
84	2134.0
85	2134.0
86	1399.5
87	665.0
88	665.0
89	665.0
90	428.5
91	192.0
92	192.0
93	192.0
94	114.0
95	36.0
96	36.0
97	36.0
98	24.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33275395681222775
2	0.010463656234013859
3	0.00959168488117937
4	0.010948084763366353
5	0.012256041792618085
6	0.07930095025500318
7	0.07697569331411122
8	0.10255351966392287
9	0.08148087863708939
10	0.08918329225379404
11	0.08443589266613961
12	0.09465733463547721
13	0.0818684214605714
14	0.08579229254832658
15	0.08409679269559286
16	0.0847265497837511
17	0.09606217737059945
18	0.09819366289975041
19	0.0935431490179665
20	0.09150854919468601
21	0.08046357872544915
22	0.09814522004681517
23	0.09364003472383699
24	0.0888441922832473
25	0.08705180672464308
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2064288.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.909143610440154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54382901618669	38.911410110790776
2	13.968254015202117	14.780967153548724
3	5.216030187824414	8.27927070851979
4	2.417044078003564	5.115349289434179
5	1.3438322122921351	3.555050575425004
6	0.8631258441474002	2.7400349545126312
7	0.5576742824452202	2.065424808842032
8	0.3944801174199665	1.6697284147229046
9	0.29377964542659774	1.3989266504730042
>10	1.283918581676042	11.710257001783305
>50	0.07131147824547568	2.5810197950533498
>100	0.04136738259704871	4.065592260008472
>500	0.003322650124097936	1.295000651759002
>1k	0.0019382125723904592	1.577548024249576
>5k	9.229583678049807E-5	0.2544196008772726
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5210	0.2523872637926491	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3059	0.14818668712892774	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2968	0.14377838751182007	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2691	0.130359717248756	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	9.68857058704987E-5	0.0	0.0	0.0	0.0
7	9.68857058704987E-5	0.0	0.0	0.0	0.0
8	9.68857058704987E-5	0.0	0.0	0.0	0.0
9	9.68857058704987E-5	0.0	0.0	4.844285293524935E-5	0.0
10	9.68857058704987E-5	0.0	0.0	4.844285293524935E-5	0.0
11	9.68857058704987E-5	0.0	0.0	4.844285293524935E-5	0.0
12	9.68857058704987E-5	0.0	0.0	4.844285293524935E-5	0.0
13	9.68857058704987E-5	0.0	0.0	4.844285293524935E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	470	0.0	12.934089	4
GGTATCA	1670	0.0	11.978761	1
TAGGACT	275	0.0	11.743565	4
TCTATAC	170	1.0913936E-11	11.174673	3
TTAGTAC	95	1.3631086E-5	10.998231	3
GTACCGA	70	0.0014937632	10.855396	6
GTATCAA	3620	0.0	10.710134	1
CGCCCTA	305	0.0	10.588717	16
ACCCTAC	135	5.466063E-8	10.584408	1
GTCCTAA	515	0.0	10.5433035	1
GTCCTAC	1010	0.0	10.469132	1
GTCTTAC	165	8.8584784E-10	10.391964	1
CGTGCGC	130	3.8510007E-7	10.229124	10
TGGCGAG	1000	0.0	10.16361	18
CTAGGAC	350	0.0	10.041242	3
CGAAATC	105	4.1121428E-5	9.951021	13
TCCTACA	1135	0.0	9.875064	2
TTAACTG	510	0.0	9.870961	5
ATTTCCG	135	6.45623E-7	9.850505	14
TAGAAAT	290	0.0	9.826005	4
>>END_MODULE