FastQCFastQC Report
Thu 26 May 2016
SRR1512844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064288
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72020.3488854268396658No Hit
TATCAACGCAGAGTACTTTTTTTTT49240.23853260785316777No Hit
GGTATCAACGCAGAGTACTTTTTTT40900.19813126850516982No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25480.12343238927901533No Hit
GTACTGGTTCACTATCGGTCAGTCA25440.12323861786727433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAA250.00604163118.99515310
TTGCCGA500.001511531113.286319
TAAAGGT1900.013.0149824
TAGGACC4200.012.6812654
GCGTGCG1457.2759576E-1212.4463069
GGTATCA17200.012.2772491
TTAGGAC6250.012.0227143
CGTGCGC1501.7644197E-1011.39709110
TAGGACG9700.011.1778154
TGGCGAG10300.010.96575118
GTAGGAC10200.010.9104123
GTTATAT1053.414907E-610.87097551
GGCGAGG4950.010.73640319
GTCCTAG2750.010.7227341
TGTAGGA11100.010.6256182
GGACGTG10050.010.5992666
GTGCGCC1803.6379788E-1110.54749211
GACGTGA5250.010.5032537
AGGACGT10350.010.4758275
GTATCAA35100.010.2167031
Produced by FastQC (version 0.11.5)