FastQCFastQC Report
Thu 26 May 2016
SRR1512843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333532
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51920.38934198804378145No Hit
TATCAACGCAGAGTACTTTTTTTTT30510.228790910154387No Hit
GGTATCAACGCAGAGTACTTTTTTT29660.2224168598878767No Hit
GTACTGGTTCACTATCGGTCAGTCA21400.16047608906272964No Hit
GGTATCAACGCAGAGTACATGGGGG14980.11233326234391075No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14380.10783393274402114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT655.330535E-513.1852981
TTAGACT655.4599426E-513.1516374
GGTATCA12950.013.0156031
TCTTATA907.4721665E-611.609162
TAATACT1406.82121E-1011.5337764
TAGGACC1950.011.2032464
GTAATAC600.0058822611.0814723
GTACTAG951.324153E-511.0263021
GTGCGCC803.775768E-410.68570411
GTATCAA28200.010.6033071
TAGAGTG909.528024E-510.5537825
GTACTGG6800.010.5029951
CGTGCGC1002.4021145E-510.44824510
TACTGGT6100.010.1212512
TGCGTTG1701.5588739E-910.05788912
CATTAGG856.601812E-410.0571342
GGTTATA951.6042827E-410.02391051
TTACACC951.6462097E-49.9983214
GTCCTAC5150.09.98498251
GACCGTG1054.108894E-59.9510827
Produced by FastQC (version 0.11.5)