##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512843_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1333532
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.583617041060883	31.0	30.0	34.0	16.0	34.0
2	28.695741084578398	31.0	29.0	34.0	16.0	34.0
3	28.869577182999734	31.0	30.0	34.0	18.0	34.0
4	32.24292330442764	35.0	33.0	37.0	22.0	37.0
5	32.42055308758995	35.0	33.0	37.0	23.0	37.0
6	32.31419418506643	35.0	33.0	37.0	22.0	37.0
7	32.21990998341247	35.0	33.0	37.0	21.0	37.0
8	32.13356784839059	35.0	33.0	37.0	19.0	37.0
9	33.38427349324951	38.0	33.0	39.0	17.0	39.0
10	33.25768410506834	38.0	33.0	39.0	17.0	39.0
11	33.30746393787326	38.0	33.0	39.0	17.0	39.0
12	33.192835267545135	38.0	33.0	39.0	17.0	39.0
13	33.15614773398764	38.0	33.0	39.0	17.0	39.0
14	33.979021875740514	39.0	33.0	41.0	16.0	41.0
15	34.13384080771965	39.0	33.0	41.0	17.0	41.0
16	34.073223589685135	39.0	33.0	41.0	16.0	41.0
17	33.817969872489	39.0	33.0	41.0	11.0	41.0
18	33.81879474958231	39.0	33.0	41.0	10.0	41.0
19	33.94712087898903	39.0	33.0	41.0	10.0	41.0
20	33.90225356421893	39.0	33.0	41.0	10.0	41.0
21	33.79863550331001	39.0	33.0	41.0	9.0	41.0
22	33.79812257973562	39.0	34.0	41.0	2.0	41.0
23	33.449549767084704	39.0	33.0	41.0	2.0	41.0
24	33.50087137016585	39.0	33.0	41.0	2.0	41.0
25	33.208597169021814	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.36581945872858057
1101	2	-0.011813939558553699
1101	3	0.3488954489562843
1101	4	0.33921569645790584
1101	5	0.2794012294788786
1101	6	0.1422539321095968
1101	7	0.01530874260596704
1101	8	0.2549902444764527
1101	9	0.15583067068426004
1101	10	0.27948386428917615
1101	11	0.4013494901294905
1101	12	0.2339565235260892
1101	13	0.47305213853955763
1101	14	0.6636374002607752
1101	15	0.505938064147486
1101	16	0.33174957968938656
1101	17	-0.19345080006149828
1101	18	0.0448239179402492
1101	19	-0.35971505568747375
1101	20	-1.3440987583800847
1101	21	-0.020840100468895173
1101	22	0.060001735130484235
1101	23	-0.43818171639682646
1101	24	0.23935321210399962
1101	25	-0.30110117623182475
1102	1	1.1576192839289483
1102	2	1.2127823343933777
1102	3	1.1977548680795778
1102	4	1.294583346837392
1102	5	1.1641583297177007
1102	6	1.212545698728185
1102	7	1.1382157295438091
1102	8	1.2743935348537079
1102	9	1.6841160669454638
1102	10	1.6075625825668993
1102	11	1.4818237337491666
1102	12	1.599197430890257
1102	13	1.4482773458152636
1102	14	1.7193687295083677
1102	15	1.6883406824228118
1102	16	1.6727284044939381
1102	17	1.9171112949838047
1102	18	1.7591805995100458
1102	19	1.6296110093815557
1102	20	1.961056768130753
1102	21	1.8754622763279158
1102	22	1.7623110560950224
1102	23	1.8649734435129588
1102	24	1.214743124199444
1102	25	1.6435022172187672
1103	1	0.7696511691883927
1103	2	0.9673616517686838
1103	3	1.1564042760927435
1103	4	1.218909465919083
1103	5	1.0621675865004576
1103	6	0.6458619631843945
1103	7	0.5381239676374463
1103	8	0.7580047028479839
1103	9	0.8594854651744583
1103	10	1.0544904321169568
1103	11	0.822831620701514
1103	12	0.9504002211685858
1103	13	1.1112656026948713
1103	14	1.182805945771669
1103	15	1.0757625616755533
1103	16	0.9838413274876885
1103	17	0.7435045548432058
1103	18	1.1671918805218127
1103	19	1.15059854093208
1103	20	1.2970377804384867
1103	21	0.9182237017339006
1103	22	0.9692084691586231
1103	23	0.24706823068731154
1103	24	0.32573284964659166
1103	25	0.6793824422451635
1104	1	0.8796532840378148
1104	2	0.8019439315763641
1104	3	0.7746054095023105
1104	4	0.9722918213373077
1104	5	0.8193140931622338
1104	6	0.6750595181938763
1104	7	0.8554101011499498
1104	8	0.6910077235267238
1104	9	0.9560599237823695
1104	10	0.8515444281530193
1104	11	0.607184823014002
1104	12	0.9808713647032263
1104	13	0.9617242255723042
1104	14	1.0814234799321056
1104	15	0.8336728939314995
1104	16	0.9090049007971714
1104	17	1.182856100835565
1104	18	0.9893052046346469
1104	19	0.5803799253911137
1104	20	0.693113752193085
1104	21	0.9606659691519326
1104	22	0.6928484151083154
1104	23	1.012972413035989
1104	24	0.9594002231346153
1104	25	0.5289038873797978
1105	1	1.1566882728631995
1105	2	1.0494949760706405
1105	3	1.2175490549568728
1105	4	1.2499075531015151
1105	5	1.1533522798205027
1105	6	1.363906350053803
1105	7	1.2350977270166155
1105	8	1.2411377818445501
1105	9	1.1420772730093205
1105	10	1.2328293312719865
1105	11	1.0883251960757718
1105	12	1.361944109313086
1105	13	1.2157833377100147
1105	14	1.3537115977442369
1105	15	1.3180516113888174
1105	16	1.2482826532845266
1105	17	1.4800578565526052
1105	18	1.4694271700186263
1105	19	1.144829278050075
1105	20	1.278743596010699
1105	21	1.146921703988454
1105	22	1.0664771124269663
1105	23	1.4442267334824521
1105	24	1.4579816180042187
1105	25	1.785511243332131
1106	1	0.7017136733209739
1106	2	0.6861839124797413
1106	3	0.5834123062630816
1106	4	0.6316122240335744
1106	5	0.6336325824071167
1106	6	0.8252626092703679
1106	7	0.7389514828512915
1106	8	0.6715061491919769
1106	9	0.9420524177483642
1106	10	0.6913677086043677
1106	11	0.453488262300759
1106	12	0.7607041555762493
1106	13	0.7869958030519228
1106	14	1.074345130729121
1106	15	0.911617146188803
1106	16	1.038880412787499
1106	17	1.1473094789297704
1106	18	1.013039106067275
1106	19	1.1164323804818466
1106	20	1.2296658833232357
1106	21	1.2066595619315876
1106	22	1.1303387057634708
1106	23	1.3343086272819207
1106	24	0.9324840247267616
1106	25	1.0266067984781913
1107	1	0.6444252662626297
1107	2	0.6794426567165992
1107	3	0.6934077698141046
1107	4	0.7947202470676231
1107	5	0.5567184786798265
1107	6	0.7959326150103649
1107	7	0.5387403991028847
1107	8	0.5681403817914585
1107	9	0.6939105102178615
1107	10	0.8078586264483505
1107	11	0.6741550277200545
1107	12	0.7482914127836509
1107	13	0.5448906129839699
1107	14	0.7815127201156855
1107	15	0.5945383415893986
1107	16	0.5195079240360414
1107	17	0.7507988414198152
1107	18	0.5495751106592692
1107	19	0.512333374797393
1107	20	0.6916476634628452
1107	21	0.4321965293617396
1107	22	0.47585271320695455
1107	23	0.7389103455777004
1107	24	0.46932045333786476
1107	25	0.7523321674118506
1108	1	0.759368582652975
1108	2	0.7365873196753228
1108	3	0.6577580816183115
1108	4	0.7415301307730786
1108	5	0.6946015809320869
1108	6	0.7114645006496332
1108	7	0.6538675602209452
1108	8	0.7807631297489834
1108	9	0.9509312055013197
1108	10	0.9770070860557354
1108	11	0.5850167250349045
1108	12	0.7679166711420464
1108	13	0.8855234025449619
1108	14	0.8694211037900956
1108	15	0.8550538608343246
1108	16	0.46694696032596994
1108	17	0.6663238522863892
1108	18	0.8907359271161468
1108	19	0.5551563338769725
1108	20	0.6113481007870973
1108	21	0.5575792989637094
1108	22	0.7656064189311991
1108	23	0.9710938177370849
1108	24	0.841449459556209
1108	25	0.965980143567478
1109	1	0.8665618125542416
1109	2	0.8992577475079528
1109	3	0.8702639584401268
1109	4	1.0344309300208465
1109	5	0.8436377821544667
1109	6	0.9921415105226927
1109	7	0.8974030562247108
1109	8	0.7135322881880981
1109	9	0.9316552299489018
1109	10	0.9097762444948501
1109	11	0.9094172892097987
1109	12	0.7890732296751892
1109	13	0.9635675962450065
1109	14	1.0555988188817693
1109	15	0.8954863949198923
1109	16	0.7123630393104534
1109	17	1.0588955495125347
1109	18	1.085846411366262
1109	19	0.9270416183151511
1109	20	0.8290255808058973
1109	21	0.799704357731926
1109	22	0.8055688072715839
1109	23	1.170905759439016
1109	24	0.8536732489309102
1109	25	0.5075880420159962
1110	1	0.3059419030638644
1110	2	0.4206841802048835
1110	3	0.3594657087480826
1110	4	0.2856227100871962
1110	5	0.1885198911389807
1110	6	0.16667064248656516
1110	7	0.11718634267479189
1110	8	0.18080068887834955
1110	9	0.3292606384635306
1110	10	0.15009097949286598
1110	11	0.12090145265241858
1110	12	0.10675123499650141
1110	13	0.19158696940152709
1110	14	0.20279974446479088
1110	15	0.21393593830068625
1110	16	0.17910351097958
1110	17	0.32588580252448196
1110	18	-0.6368326051623754
1110	19	-0.15324496046840608
1110	20	0.009064479456661445
1110	21	0.20536068381871075
1110	22	0.2296816369974053
1110	23	0.32895549508052824
1110	24	0.25799530664590264
1110	25	0.38814081784578036
1111	1	0.35389414441728917
1111	2	0.5001166446005634
1111	3	0.45175444679986043
1111	4	0.368497078928705
1111	5	0.5600374898262217
1111	6	0.38664698968233324
1111	7	0.33094592693329616
1111	8	0.4523088053723612
1111	9	0.5373592216658238
1111	10	0.48441138280215057
1111	11	0.21873661525886234
1111	12	0.050557387359546624
1111	13	0.3668429227702035
1111	14	0.3658045724815153
1111	15	0.42872144318796757
1111	16	0.6215624017210075
1111	17	0.07492638894545678
1111	18	0.5135846113398017
1111	19	0.5583416211745913
1111	20	0.6112410378946294
1111	21	0.6588880597007574
1111	22	0.39700217990564113
1111	23	0.843500784405947
1111	24	0.7178230731416235
1111	25	0.7457604185927664
1112	1	0.2401550534408372
1112	2	0.3461244124745093
1112	3	0.2992363140010852
1112	4	0.40774038335054286
1112	5	0.28572452306362095
1112	6	0.010347070797145363
1112	7	-0.0774333572498378
1112	8	0.10077152567578196
1112	9	0.03991168235596376
1112	10	0.22409908095801967
1112	11	0.0923883356862234
1112	12	0.19888338626184066
1112	13	0.403189831850149
1112	14	0.2226215277713166
1112	15	0.017898222976015177
1112	16	0.23945657738931914
1112	17	0.5540244080261232
1112	18	0.29810250282694284
1112	19	0.4463107280395846
1112	20	0.20992733211515002
1112	21	0.10306867883558368
1112	22	0.021076031757353064
1112	23	0.289185666822263
1112	24	0.16321943077858236
1112	25	0.23970762773847554
1113	1	-0.28529171486944804
1113	2	-0.0875384753588726
1113	3	0.0014266377386320528
1113	4	-0.005751451045583167
1113	5	-0.044696615659795214
1113	6	-0.5903881397066151
1113	7	-0.6607879027839054
1113	8	-0.4927086748123166
1113	9	-0.49335364072553745
1113	10	-0.44827344902334687
1113	11	-0.32914685907239516
1113	12	-0.5474633041974784
1113	13	-0.3824678775820658
1113	14	-0.08546841195978772
1113	15	-0.3931975074672067
1113	16	-0.38190717030358456
1113	17	-0.14637096990094278
1113	18	-0.37919847424638675
1113	19	-0.8796477572780788
1113	20	-0.6253334686126237
1113	21	-0.3036525272420363
1113	22	-0.9722604943402189
1113	23	-4.6464670129128685
1113	24	-2.1519059617564764
1113	25	-1.2552150023994706
1114	1	-0.9011164257641511
1114	2	-1.0615997609995844
1114	3	-0.9743821548078344
1114	4	-0.9928575889733899
1114	5	-0.8820707904807179
1114	6	-1.4502434700123388
1114	7	-1.3213747748284774
1114	8	-1.288796974826365
1114	9	-1.7017447170867896
1114	10	-1.2227525913052872
1114	11	-1.4499355195902126
1114	12	-2.374360630851349
1114	13	-1.5637392386008386
1114	14	-3.5081731265999885
1114	15	-2.1705969643706204
1114	16	-2.137585104663806
1114	17	-2.047625710291406
1114	18	-1.7649339955722922
1114	19	-2.03767481847008
1114	20	-1.6230136034504028
1114	21	-1.3441495074377414
1114	22	-1.4892053419454427
1114	23	-5.866952522534106
1114	24	-3.1548859701838374
1114	25	-2.9655034054145943
1115	1	-1.7453728225584193
1115	2	-1.5144948144937835
1115	3	-1.3439557335322654
1115	4	-1.2734465656169718
1115	5	-1.1987761608064318
1115	6	-1.3936608981809222
1115	7	-1.814206302848934
1115	8	-2.2111597708499637
1115	9	-3.4748765775825703
1115	10	-4.143802590889546
1115	11	-2.7489105358338684
1115	12	-2.925854156550777
1115	13	-2.4221684204074663
1115	14	-3.0785990964978467
1115	15	-2.6540844986248437
1115	16	-2.7591823403316695
1115	17	-2.8378609849844416
1115	18	-5.5177244007075466
1115	19	-3.3139414971734844
1115	20	-3.9905849656726993
1115	21	-3.4281169274696452
1115	22	-2.745544407379768
1115	23	-3.192600398029878
1115	24	-2.998509012778449
1115	25	-3.3969741721746622
1116	1	-1.6272257474313783
1116	2	-1.4698367910240293
1116	3	-1.910915778068059
1116	4	-1.607133036776073
1116	5	-1.7692823274014025
1116	6	-4.066389883091592
1116	7	-2.6816609543637284
1116	8	-2.9988597306849947
1116	9	-3.8471499552471826
1116	10	-3.353402375154438
1116	11	-2.824681309671803
1116	12	-2.6547667750332735
1116	13	-4.311846023407782
1116	14	-6.794385451204697
1116	15	-4.936188115683613
1116	16	-3.7954237933856234
1116	17	-5.019084183071932
1116	18	-3.627307229600735
1116	19	-3.306560559910025
1116	20	-4.07981659245857
1116	21	-4.753080537627362
1116	22	-4.3607632921919155
1116	23	-3.259840931498662
1116	24	-4.2842434098532785
1116	25	-4.059173704796155
1201	1	0.4362491656336971
1201	2	0.628480762524962
1201	3	0.8687684801359694
1201	4	0.7719492518770963
1201	5	0.17575288489513952
1201	6	0.6383761962164343
1201	7	0.6194100254439903
1201	8	0.767307973356381
1201	9	1.0138235697813087
1201	10	0.8703724999222686
1201	11	0.9616041583156871
1201	12	0.8460901549220168
1201	13	1.0170141793916656
1201	14	1.0429629605892288
1201	15	1.0444458517632782
1201	16	1.079506074239518
1201	17	1.2204251825222912
1201	18	1.0789221324868734
1201	19	1.366902372207683
1201	20	1.3286065979800483
1201	21	1.4125794372418667
1201	22	1.3284794242687354
1201	23	1.5673879630397778
1201	24	1.4781809786800792
1201	25	1.440613706442555
1202	1	0.9864264705982357
1202	2	0.8821624479650936
1202	3	0.9939050144402124
1202	4	1.189790682453193
1202	5	1.0141360911757218
1202	6	1.2194965360351446
1202	7	1.1997983896871744
1202	8	1.0929451334428535
1202	9	1.3120704313886193
1202	10	1.2337016613740843
1202	11	1.1867312110710273
1202	12	1.3355646874816571
1202	13	1.3888151047908437
1202	14	1.7502182418119716
1202	15	1.456858147435895
1202	16	1.1356624439440708
1202	17	1.5319035456276922
1202	18	1.423590657537126
1202	19	1.479769782227784
1202	20	1.3667963806629473
1202	21	1.5311993029831399
1202	22	1.5713957590811916
1202	23	1.6168171737956456
1202	24	1.7783867282913306
1202	25	2.0404420904073604
1203	1	1.1515937741774493
1203	2	1.2165364659878612
1203	3	1.1428983178738257
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2211	18	0.09953589638708138
2211	19	0.17609149936355806
2211	20	0.03882694029757516
2211	21	0.2248654887862358
2211	22	0.2931166838548478
2211	23	0.37934003483839973
2211	24	0.17357294817690416
2211	25	0.6464288418612725
2212	1	-0.06851103763770183
2212	2	0.06646500338662165
2212	3	-0.2449053981814373
2212	4	-0.006896650466693188
2212	5	-0.31552167161636646
2212	6	-0.5370873765399686
2212	7	-0.3629396462017027
2212	8	-0.24355364141815983
2212	9	-0.09982415228989083
2212	10	-0.4281281866791957
2212	11	-0.5979786028497571
2212	12	-0.21036927020105622
2212	13	-0.31674722893536256
2212	14	-0.25276236145955266
2212	15	-0.4399302283495672
2212	16	-0.6408581981964687
2212	17	-0.34208591052855297
2212	18	-0.10404431278585236
2212	19	-0.4163247013754443
2212	20	-0.21383518101835364
2212	21	-0.09237109942825583
2212	22	0.046163148544536625
2212	23	0.30700813697881557
2212	24	0.09220983462454768
2212	25	0.05043234089967541
2213	1	-0.31238691131363794
2213	2	-0.1918426648592373
2213	3	-0.3348419044152031
2213	4	-0.2520555155928079
2213	5	-0.20640249381263231
2213	6	0.045492155182799365
2213	7	-0.3149055550928601
2213	8	-0.10117246517664569
2213	9	-0.5505210422356868
2213	10	-0.08088800427940868
2213	11	-0.3094520124970046
2213	12	-0.2732964739017021
2213	13	-0.32652144677933137
2213	14	-0.49365195027577613
2213	15	-0.22420061344531916
2213	16	-0.1866156724724135
2213	17	-0.2429793846025703
2213	18	-0.2791783924913318
2213	19	-0.25091210457640756
2213	20	-0.06509088101154958
2213	21	-0.1472152090934884
2213	22	-0.4297991851882941
2213	23	-0.20338763196643583
2213	24	0.12332029268382172
2213	25	0.1793104434154884
2214	1	-2.3870792991504644
2214	2	-1.0566181358464632
2214	3	-1.2875649295667628
2214	4	-3.0357196676868377
2214	5	-1.2843794250882503
2214	6	-1.093425930671323
2214	7	-0.9981062507841258
2214	8	-0.7615582274569519
2214	9	-0.6975263548039123
2214	10	-0.6833361890060772
2214	11	-0.603740840443308
2214	12	-0.5564820618907049
2214	13	-1.0679701648815794
2214	14	-0.7981753252996171
2214	15	-1.116735105527134
2214	16	-1.0394979733386194
2214	17	-3.3131343951549326
2214	18	-3.048201891416749
2214	19	-1.7478956307325433
2214	20	-1.7373679710101584
2214	21	-1.7599361707540062
2214	22	-2.592321213415744
2214	23	-1.4958497176215815
2214	24	-2.598784578721073
2214	25	-2.615753114872142
2215	1	-1.951228809698673
2215	2	-3.332249608431688
2215	3	-2.0214787143689357
2215	4	-3.4307702645695635
2215	5	-2.2484450403105
2215	6	-1.9706711132407477
2215	7	-2.1323067208412603
2215	8	-1.6449916846134833
2215	9	-3.799234113647664
2215	10	-2.222784070730981
2215	11	-1.7935260581149457
2215	12	-1.8442790585971238
2215	13	-1.7799883457894765
2215	14	-1.462618820417262
2215	15	-1.7191531438176568
2215	16	-1.663982868670658
2215	17	-1.9664075996054535
2215	18	-1.8324056134120639
2215	19	-1.815757029852037
2215	20	-1.592275613626363
2215	21	-2.1952406153346615
2215	22	-2.0866474001275286
2215	23	-1.9504776935544328
2215	24	-1.7684922364973943
2215	25	-3.043146693440608
2216	1	-3.162885495343069
2216	2	-3.4467521329091717
2216	3	-4.706775407404823
2216	4	-5.340314017584319
2216	5	-5.010581591996612
2216	6	-3.7279920295945175
2216	7	-5.523823455715188
2216	8	-3.5369015767509993
2216	9	-4.017090858578943
2216	10	-2.9833468541066814
2216	11	-3.3991655122837017
2216	12	-3.5296447905497708
2216	13	-3.216119031218323
2216	14	-4.234188184363774
2216	15	-3.6540751925008017
2216	16	-3.96841669372969
2216	17	-3.556560007001597
2216	18	-3.2433626135594835
2216	19	-3.927409819240733
2216	20	-4.4681593630274
2216	21	-4.052364918878958
2216	22	-3.8609600926475593
2216	23	-3.8847025175336753
2216	24	-4.270278703912734
2216	25	-4.289239443921428
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73433.0
3	1.0
4	660.0
5	3985.0
6	4991.0
7	4622.0
8	4184.0
9	3909.0
10	3343.0
11	3141.0
12	3047.0
13	2848.0
14	2853.0
15	2898.0
16	3016.0
17	3232.0
18	3616.0
19	4428.0
20	5537.0
21	7253.0
22	8942.0
23	10852.0
24	12387.0
25	14278.0
26	15390.0
27	16920.0
28	19228.0
29	22719.0
30	27314.0
31	33546.0
32	41612.0
33	52235.0
34	67025.0
35	87126.0
36	116202.0
37	174193.0
38	472566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.767050456499724	17.59220662349721	12.535721648786405	24.105021271216664
2	16.17458684193948	19.37223073853972	39.39858465802902	25.05459776149178
3	19.686547553337014	24.49310338621037	28.405484292879603	27.414864767573015
4	13.308769266767865	15.547134208900063	36.07235425053848	35.071742273793596
5	14.379804555374728	37.716612793148194	33.40288142076093	14.500701230716157
6	33.68966940598146	34.37817554129611	17.479680288190927	14.452474764531503
7	29.930337490168295	31.295893799571306	20.3195829555757	18.454185754684698
8	27.424736861465377	33.85371742063054	19.885473461687734	18.836072256216347
9	28.150271880875273	14.447250606994224	19.068587532882763	38.333889979247736
10	17.517505796484652	27.440922393533455	31.63518287918467	23.406388930797217
11	35.76602068541063	22.24364651665491	22.61840070852785	19.371932089406606
12	24.616679202566633	24.173643983443604	29.137710239052854	22.07196657493691
13	29.824434997718374	20.94448747508226	25.15506040300694	24.076017124192425
14	22.919620480711423	21.13993891844058	26.120210130171724	29.82023047067627
15	25.05090564386539	28.454022052482536	22.86059321493252	23.634479088719548
16	24.041238599040486	27.002635955879263	25.19814702509262	23.75797841998763
17	23.177408034350357	27.140385315522593	25.865308466357146	23.816898183769908
18	23.141048016905195	26.061998220901035	27.558918578072046	23.23803518412173
19	24.527025809783456	26.13465765105749	26.780953124437023	22.557363414722026
20	25.177615060406012	25.317230068268735	26.711803848420136	22.793351022905117
21	25.223784374458226	25.371110182142132	26.17093246053612	23.234172982863523
22	25.230271701841122	25.70748024580331	25.96083541897814	23.101412633377425
23	24.4424177597628	25.714274991086338	26.41963632269324	23.423670926457618
24	24.435048709621004	25.379667833335713	26.611463180120136	23.573820276923147
25	24.310239485271047	25.438761095690527	26.55504798562458	23.695951433413846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	216.0
1	216.0
2	518.5
3	821.0
4	821.0
5	821.0
6	1410.0
7	1999.0
8	1999.0
9	1999.0
10	2179.5
11	2360.0
12	2360.0
13	2360.0
14	3075.0
15	3790.0
16	3790.0
17	3790.0
18	7224.0
19	10658.0
20	10658.0
21	10658.0
22	16825.0
23	22992.0
24	22992.0
25	22992.0
26	34416.0
27	45840.0
28	45840.0
29	45840.0
30	58685.0
31	71530.0
32	71530.0
33	71530.0
34	83648.0
35	95766.0
36	95766.0
37	95766.0
38	107350.5
39	118935.0
40	118935.0
41	118935.0
42	132130.5
43	145326.0
44	145326.0
45	145326.0
46	162213.0
47	179100.0
48	179100.0
49	179100.0
50	179562.5
51	180025.0
52	180025.0
53	180025.0
54	167483.0
55	154941.0
56	154941.0
57	154941.0
58	137833.5
59	120726.0
60	120726.0
61	120726.0
62	103419.0
63	86112.0
64	86112.0
65	86112.0
66	67720.0
67	49328.0
68	49328.0
69	49328.0
70	37346.0
71	25364.0
72	25364.0
73	25364.0
74	18263.5
75	11163.0
76	11163.0
77	11163.0
78	7792.5
79	4422.0
80	4422.0
81	4422.0
82	2961.0
83	1500.0
84	1500.0
85	1500.0
86	988.5
87	477.0
88	477.0
89	477.0
90	297.5
91	118.0
92	118.0
93	118.0
94	66.0
95	14.0
96	14.0
97	14.0
98	11.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3378996529517102
2	0.010798391039735079
3	0.00929861450643854
4	0.011098346346394387
5	0.012148189919701964
6	0.08113791045134275
7	0.08308761994462825
8	0.10790892157068596
9	0.0860121841845565
10	0.09441093277101713
11	0.08938668138447371
12	0.10183482661083498
13	0.08608717301122132
14	0.09111142439776473
15	0.08736198306452339
16	0.0890867260778144
17	0.1032596143174667
18	0.10475939085076323
19	0.09928520650423088
20	0.09726050818428054
21	0.08331258642462273
22	0.10573424559740599
23	0.09801039645092882
24	0.09366104450436885
25	0.09433594394435228
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1333532.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.52182045519464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88613494988857	47.13810675386216
2	12.886568080515449	15.598371193051964
3	4.2104879953928975	7.64479195457764
4	1.8389644782372205	4.45189911901415
5	0.9454436227600272	2.860998459359557
6	0.5528799874325467	2.0076781999597717
7	0.365269896282489	1.547475935634746
8	0.24214001730744186	1.1723803721998975
9	0.18304769174879418	0.9970541581282503
>10	0.8116256955240677	8.343514097653866
>50	0.04590942528007888	1.9481652543528867
>100	0.026648840686510477	3.1596315697014257
>500	0.003503100780230667	1.4723617638991076
>1k	0.0012511074215109527	1.2644365695732165
>5k	1.2511074215109527E-4	0.3931345990313277
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5192	0.38934198804378145	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3051	0.228790910154387	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2966	0.2224168598878767	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2140	0.16047608906272964	No Hit
GGTATCAACGCAGAGTACATGGGGG	1498	0.11233326234391075	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1438	0.10783393274402114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.499776533296539E-4
7	0.0	0.0	0.0	0.0	1.499776533296539E-4
8	0.0	0.0	0.0	0.0	1.499776533296539E-4
9	0.0	0.0	0.0	7.498882666482695E-5	1.499776533296539E-4
10	0.0	0.0	0.0	7.498882666482695E-5	1.499776533296539E-4
11	0.0	0.0	0.0	7.498882666482695E-5	1.499776533296539E-4
12	0.0	0.0	0.0	7.498882666482695E-5	1.499776533296539E-4
13	0.0	0.0	0.0	7.498882666482695E-5	1.499776533296539E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTAT	65	5.330535E-5	13.185298	1
TTAGACT	65	5.4599426E-5	13.151637	4
GGTATCA	1295	0.0	13.015603	1
TCTTATA	90	7.4721665E-6	11.60916	2
TAATACT	140	6.82121E-10	11.533776	4
TAGGACC	195	0.0	11.203246	4
GTAATAC	60	0.00588226	11.081472	3
GTACTAG	95	1.324153E-5	11.026302	1
GTGCGCC	80	3.775768E-4	10.685704	11
GTATCAA	2820	0.0	10.603307	1
TAGAGTG	90	9.528024E-5	10.553782	5
GTACTGG	680	0.0	10.502995	1
CGTGCGC	100	2.4021145E-5	10.448245	10
TACTGGT	610	0.0	10.121251	2
TGCGTTG	170	1.5588739E-9	10.057889	12
CATTAGG	85	6.601812E-4	10.057134	2
GGTTATA	95	1.6042827E-4	10.0239105	1
TTACACC	95	1.6462097E-4	9.998321	4
GTCCTAC	515	0.0	9.9849825	1
GACCGTG	105	4.108894E-5	9.951082	7
>>END_MODULE