FastQCFastQC Report
Thu 26 May 2016
SRR1512843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333532
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65320.4898270157746496No Hit
TATCAACGCAGAGTACTTTTTTTTT49210.36902001601761336No Hit
GGTATCAACGCAGAGTACTTTTTTT40040.3002552619659671No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22920.17187439071578334No Hit
GTACTGGTTCACTATCGGTCAGTCA18940.14202883770318223No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14660.1099336198906363No Hit
GGTATCAACGCAGAGTACATGGGGG14660.1099336198906363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC400.00527539814.2512958
TAGGACC2150.013.2694694
CGTGCGC1352.7284841E-1112.66211610
TTAGGAC3900.012.4368093
GGTATCA13550.012.4219111
CCCGTAC1900.011.99163916
GTCCTAA2550.011.9334011
TCCTAGA1406.675691E-1011.5484652
CGTGAAA3050.011.522619
CTACACA1751.8189894E-1211.4117434
ACCGTGC752.071643E-411.4010368
GCGTGCG1354.7366484E-911.2573259
TCAATAC855.2617565E-511.1888243
CCTAGAC1201.2550663E-711.0955843
AAGACCG600.005830056511.094755
ACGTGAA3100.011.033268
GTATAGA1303.2159733E-810.9724181
GTCTAGA1053.423458E-610.8679191
ACTGCGC700.001490030610.85812958
GGCGTGC1408.3309715E-910.85812958
Produced by FastQC (version 0.11.5)