FastQCFastQC Report
Thu 26 May 2016
SRR1512842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589366
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT104680.6586274023730218No Hit
TATCAACGCAGAGTACTTTTTTTTT62810.39518902505779036No Hit
GGTATCAACGCAGAGTACTTTTTTT61330.38587713591457223No Hit
GTCCTACAGTGGACATTTCTAAATT60650.3815987003622828No Hit
GTCCTAAAGTGTGTATTTCTCATTT55690.3503912880985248No Hit
CTGTAGGACGTGGAATATGGCAAGA52520.3304462282444698No Hit
CTTTAGGACGTGAAATATGGCGAGG45170.2842013733778123No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29590.18617486469447567No Hit
GTCCTACAGTGTGCATTTCTCATTT25870.16276930549665716No Hit
CTGAAGGACCTGGAATATGGCGAGA23160.14571848145738614No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22750.1431388364920352No Hit
GTACTGGTTCACTATCGGTCAGTCA21720.13665826499371447No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20550.12929683911698123No Hit
GTCCTTCAGTGTGCATTTCTCATTT19370.1218724950703614No Hit
ATTTAGAAATGTCCACTGTAGGACG17190.10815633403508065No Hit
TTTCTAAATTTTCCACCTTTTTCAG17190.10815633403508065No Hit
CTGTAGGACCTGGAATATGGCGAGA16330.10274537142483228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT250.006038028718.9967887
GGACCGT400.00528509514.2475916
TAGGACC7650.013.6573924
AGGACCG500.001501840813.2973335
CCCGTAC1850.012.83647616
GGTATCA18300.012.704541
GGCGAGG11250.012.49527119
CCGTACA1950.012.17819617
AGGACGA1353.7289283E-1011.9605645
CCCCGTA2000.011.87336515
ACCGTGC600.00588315211.081468
TGTAGGA26750.011.0781522
TTAGGAC17000.010.8948733
GTATTAG1053.3439574E-610.88960551
TTAGACC700.00149393110.8549644
TCAGTAC700.00149393110.8549643
AGGACCT16300.010.8383175
GGACGAG1502.1373125E-910.7648466
GTATCAA40950.010.72672751
GGACCTG15350.010.70502956
Produced by FastQC (version 0.11.5)