##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512842_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1589366
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.682136147369455	31.0	30.0	34.0	20.0	34.0
2	28.842714013008962	31.0	30.0	34.0	18.0	34.0
3	28.996151295548035	31.0	30.0	34.0	22.0	34.0
4	32.36561245175749	35.0	33.0	37.0	22.0	37.0
5	32.55044401352489	35.0	33.0	37.0	25.0	37.0
6	32.43183256719975	36.0	33.0	37.0	23.0	37.0
7	32.32422047533419	36.0	33.0	37.0	22.0	37.0
8	32.22898690421212	36.0	33.0	37.0	20.0	37.0
9	33.43888128977215	38.0	33.0	39.0	18.0	39.0
10	33.3194984666842	38.0	33.0	39.0	17.0	39.0
11	33.330794165723944	38.0	33.0	39.0	17.0	39.0
12	33.235668813854076	38.0	33.0	39.0	17.0	39.0
13	33.175885227191216	38.0	33.0	39.0	17.0	39.0
14	34.01298945617309	39.0	33.0	41.0	16.0	41.0
15	34.20001874961463	39.0	33.0	41.0	17.0	41.0
16	34.14757456746904	39.0	33.0	41.0	17.0	41.0
17	33.9554986076209	39.0	33.0	41.0	11.0	41.0
18	33.94630311709197	39.0	33.0	41.0	11.0	41.0
19	34.073317914187164	39.0	34.0	41.0	11.0	41.0
20	34.05661188171887	39.0	34.0	41.0	11.0	41.0
21	33.957132592492854	39.0	34.0	41.0	10.0	41.0
22	33.971998268491966	39.0	34.0	41.0	9.0	41.0
23	33.651128814885936	39.0	33.0	41.0	2.0	41.0
24	33.72106739416849	39.0	34.0	41.0	2.0	41.0
25	33.44960380428422	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5533606905901465
1101	2	0.31670526055851767
1101	3	0.5907868537300232
1101	4	0.652429757478366
1101	5	0.5334620397333012
1101	6	0.5810063285605693
1101	7	0.21184359537240027
1101	8	0.5464091439509957
1101	9	0.6693498153492143
1101	10	0.7637321118555178
1101	11	0.7615018350027611
1101	12	0.6093698074553728
1101	13	0.9900790523567622
1101	14	0.8588866694081574
1101	15	0.772126233720897
1101	16	0.7791058663549819
1101	17	0.18516050800870687
1101	18	0.4621460049874173
1101	19	-0.2600353917280742
1101	20	-0.7108771396923146
1101	21	0.30044029354611723
1101	22	0.36758690708101227
1101	23	-0.10301009208830436
1101	24	0.366465267339386
1101	25	0.1477618071949678
1102	1	1.009500499305485
1102	2	1.0930037478771588
1102	3	0.9853600265768101
1102	4	1.1677085644877252
1102	5	0.9577024668284864
1102	6	0.8192757163928945
1102	7	0.8353937453161109
1102	8	0.8073723785726941
1102	9	1.100995506714284
1102	10	1.011146174084523
1102	11	0.9233065518440711
1102	12	1.0707213821008565
1102	13	1.0245091874164771
1102	14	1.2191859576879551
1102	15	1.1671481340169976
1102	16	1.0981837204097715
1102	17	1.2816045873606257
1102	18	1.2351093089837093
1102	19	0.9896126899068989
1102	20	1.308161296982874
1102	21	1.5748666130034792
1102	22	1.3041520450891468
1102	23	1.2166437287171306
1102	24	0.652536111407386
1102	25	1.1160926230716584
1103	1	0.6558707288181225
1103	2	0.5808481014478524
1103	3	0.7907049109281665
1103	4	0.9012015935683522
1103	5	0.73351640743644
1103	6	0.39701969857097197
1103	7	0.2704209612869164
1103	8	0.5673247467775511
1103	9	0.7069059417082713
1103	10	0.9005748694718108
1103	11	0.7761696904347488
1103	12	0.699318151877435
1103	13	0.7915190922451956
1103	14	0.9349513741738491
1103	15	0.8646319259755941
1103	16	0.9027132025832358
1103	17	0.46068613680937887
1103	18	0.7815227701967231
1103	19	0.681525900345278
1103	20	0.9040721319854796
1103	21	0.7717059295426054
1103	22	0.766164457664182
1103	23	0.04589512797804218
1103	24	0.30937025860440315
1103	25	0.534665638490452
1104	1	0.8238541995984434
1104	2	0.7315468695473903
1104	3	0.7242888143244954
1104	4	0.8938522745459423
1104	5	0.6262033135633089
1104	6	0.5808549695357001
1104	7	0.6981318508551198
1104	8	0.4271927719026536
1104	9	0.7840533937091791
1104	10	0.9133890942689504
1104	11	0.5718609221082289
1104	12	0.834904499321091
1104	13	0.962132643825079
1104	14	1.2207589066737299
1104	15	0.8476176082997497
1104	16	0.8472609648750904
1104	17	1.0287986194252952
1104	18	0.8439289482870436
1104	19	0.44300951805062994
1104	20	0.5840120491553407
1104	21	0.8132929223521757
1104	22	0.5374478831093938
1104	23	0.8044408584715157
1104	24	0.7921855508318529
1104	25	0.47883381888372156
1105	1	0.5190054095744507
1105	2	0.6571859205233679
1105	3	0.8508327555584643
1105	4	0.9151194171068866
1105	5	0.658866741779299
1105	6	0.8467460865049858
1105	7	0.8125612220230352
1105	8	0.8012708501586303
1105	9	0.322240947395386
1105	10	0.5206356213267966
1105	11	0.5688322795917173
1105	12	0.6740087912555737
1105	13	0.6257163646864328
1105	14	0.879151726791612
1105	15	0.8031061897991094
1105	16	0.7406068373314767
1105	17	1.1498406501708303
1105	18	0.9319965410716122
1105	19	0.6730234394392127
1105	20	0.8094348743086215
1105	21	0.6865860712517957
1105	22	0.5851952839565229
1105	23	0.8994173105908274
1105	24	1.0589726720664103
1105	25	1.360753785709349
1106	1	0.46048764039123213
1106	2	0.5224390113490358
1106	3	0.4836235890301559
1106	4	0.5036829153733535
1106	5	0.3697183835219633
1106	6	0.5513475726429107
1106	7	0.4862328386642716
1106	8	0.4449296533699112
1106	9	0.5775822567541127
1106	10	0.23365786329671323
1106	11	0.16905158490494188
1106	12	0.4560529434422804
1106	13	0.41967842721945203
1106	14	0.8222626332978678
1106	15	0.41353408260008706
1106	16	0.5183380461210376
1106	17	0.7674158983327075
1106	18	0.5142556607313935
1106	19	0.5411375191366403
1106	20	0.6818479979857912
1106	21	0.6701812456795508
1106	22	0.6459649114994832
1106	23	0.8350275721488458
1106	24	0.5058329846016889
1106	25	0.6221900659686455
1107	1	0.530187034648975
1107	2	0.5993444655263609
1107	3	0.6196358828797557
1107	4	0.6709686518573506
1107	5	0.4751937104571624
1107	6	0.6117900266774896
1107	7	0.23470637389965532
1107	8	0.5626496412698074
1107	9	0.3731729393133705
1107	10	0.5317069998857349
1107	11	0.42236343167948576
1107	12	0.4527579065455356
1107	13	0.3368557456325334
1107	14	0.6749957690042052
1107	15	0.34642920084610296
1107	16	0.5239011755231076
1107	17	0.7230384226363356
1107	18	0.5817829329718407
1107	19	0.5167622185707614
1107	20	0.6169007975487162
1107	21	0.33128929566302645
1107	22	0.3845685288648468
1107	23	0.5733742653863985
1107	24	0.5196766522868757
1107	25	0.6738127118148327
1108	1	0.7951181784385923
1108	2	0.7126926927109807
1108	3	0.6702147883885843
1108	4	0.7543810192519373
1108	5	0.7272347424821319
1108	6	0.7509248435217444
1108	7	0.8611373606339399
1108	8	0.8161710879671418
1108	9	0.899495658426531
1108	10	0.9756211746102608
1108	11	0.6970493680439347
1108	12	0.905957227047935
1108	13	0.7606895537489748
1108	14	1.00741744353666
1108	15	0.8775990047852034
1108	16	0.6526741024542488
1108	17	1.016576062454277
1108	18	1.0625162012259608
1108	19	1.078732518547163
1108	20	1.0755823690627153
1108	21	0.9936633095819971
1108	22	1.078735287044637
1108	23	1.377759429582646
1108	24	1.304957348718382
1108	25	1.4305446414453655
1109	1	0.9917253601407481
1109	2	0.9500412450846838
1109	3	1.065135006608699
1109	4	1.1262719008915028
1109	5	0.8573927698235551
1109	6	1.0022639558633486
1109	7	0.8101056995593012
1109	8	0.8241804796587786
1109	9	0.8236072133834824
1109	10	1.0326771544958788
1109	11	1.0662838968349106
1109	12	0.9091674192338957
1109	13	1.0424420823184093
1109	14	1.35945314786089
1109	15	1.003166171113314
1109	16	0.7062001906595796
1109	17	1.114973368556015
1109	18	1.158155876763594
1109	19	1.0930143075535028
1109	20	1.0707747173904423
1109	21	1.1833410492294618
1109	22	1.015211472027275
1109	23	1.4230338306539636
1109	24	1.0548040605215974
1109	25	0.8158949311100088
1110	1	0.45389523709955526
1110	2	0.3675331759559235
1110	3	0.24574094565411642
1110	4	0.26186153502950305
1110	5	0.2678331779015508
1110	6	0.25516217622094217
1110	7	0.22027515275121345
1110	8	0.35780165110045914
1110	9	0.3099861133631876
1110	10	0.3454626540497969
1110	11	0.2340449970575662
1110	12	0.07464452302523483
1110	13	0.07565049766233756
1110	14	0.01553774411281239
1110	15	-0.02445753816831342
1110	16	0.2635127288625796
1110	17	0.2516097723939623
1110	18	-0.34588151471309914
1110	19	-0.026353595318170164
1110	20	0.10632724216622336
1110	21	0.19760479293619682
1110	22	0.1858289813373517
1110	23	0.26808468565724297
1110	24	0.1833327978093351
1110	25	0.3126397526130873
1111	1	0.2898564513149786
1111	2	0.2764860573437211
1111	3	0.38759772763340905
1111	4	0.30792233147314363
1111	5	0.46564561729132237
1111	6	0.22395393406323905
1111	7	0.06338168039066261
1111	8	0.2822305912261882
1111	9	0.3468517065074934
1111	10	0.4148519171603482
1111	11	0.0467312898357406
1111	12	-0.300650421948113
1111	13	0.28597943195758546
1111	14	0.3088454233316753
1111	15	0.26885906525549075
1111	16	0.43278258611100284
1111	17	-0.167069005013758
1111	18	0.23390801683753892
1111	19	0.15786609005005658
1111	20	0.42740674969289927
1111	21	0.3251263270427245
1111	22	-0.125000779339274
1111	23	0.274240899700132
1111	24	0.3781630653807966
1111	25	0.36283870288860953
1112	1	0.3638596536809793
1112	2	0.3700256631815577
1112	3	0.31246485811160696
1112	4	0.38318121726238985
1112	5	0.3149800304228165
1112	6	0.12842464483679095
1112	7	0.0691521504193986
1112	8	0.1630000907504865
1112	9	0.057699616817359356
1112	10	0.25512808987310365
1112	11	0.14602105976348412
1112	12	0.2108343781608184
1112	13	0.15239173687872665
1112	14	0.16895960243125074
1112	15	-0.08064944143979602
1112	16	0.17102320790273495
1112	17	0.5533534540712708
1112	18	0.20334966921539177
1112	19	0.44732874115759813
1112	20	0.1054229430818765
1112	21	0.2827660483257972
1112	22	0.13231995953412223
1112	23	0.3915975800852536
1112	24	0.6464475181387286
1112	25	0.3493940810658174
1113	1	0.16249053925156076
1113	2	0.18142374519955595
1113	3	0.26013780124109687
1113	4	0.29827618481632356
1113	5	0.1483043978148757
1113	6	-0.17081638063590532
1113	7	-0.20730216324691497
1113	8	0.06501739714466481
1113	9	0.07802464586549007
1113	10	0.006348597624999286
1113	11	-0.09051718727038605
1113	12	-0.10699334750812284
1113	13	-0.03903916352364689
1113	14	0.38975304872328564
1113	15	0.06037676068777387
1113	16	-0.06557354569709162
1113	17	0.10389826417415549
1113	18	-0.06579998571744028
1113	19	-0.44034017185727237
1113	20	-0.20511197713187812
1113	21	6.469313889496675E-4
1113	22	-0.40357906147627176
1113	23	-4.4141776410315785
1113	24	-1.765420318416112
1113	25	-0.895320496551733
1114	1	-0.6306205578920547
1114	2	-0.6897588462434889
1114	3	-0.7551818831865731
1114	4	-0.6322649459723308
1114	5	-0.6187854175181791
1114	6	-1.2134007867229393
1114	7	-0.951380027390389
1114	8	-0.8205177957947001
1114	9	-1.0136965854958007
1114	10	-0.7246729818262878
1114	11	-0.8727236594762289
1114	12	-1.7565731087370757
1114	13	-0.9334628396293496
1114	14	-2.8619366027909763
1114	15	-1.6588331378782613
1114	16	-1.6712185431214195
1114	17	-1.3700861075262338
1114	18	-1.2532283399371735
1114	19	-1.43896853033376
1114	20	-1.016406095717933
1114	21	-0.7991166853415876
1114	22	-0.7918804840659064
1114	23	-5.091837559734088
1114	24	-2.314239383119215
1114	25	-2.2814504720986335
1115	1	-1.4442760874755542
1115	2	-1.2841592934820767
1115	3	-1.0992033670149368
1115	4	-1.0936020312345995
1115	5	-1.0272642098610518
1115	6	-1.1226629171696416
1115	7	-1.450973270388964
1115	8	-1.6655331431510625
1115	9	-2.9631958949794672
1115	10	-3.6632479099952917
1115	11	-2.4173929898571487
1115	12	-2.6995134836311507
1115	13	-1.9593943484560619
1115	14	-2.786071922110221
1115	15	-2.106261780700436
1115	16	-2.3640416555306984
1115	17	-2.0916387934106524
1115	18	-4.934727725654689
1115	19	-2.917649875310641
1115	20	-3.179104308140026
1115	21	-2.9054159660257994
1115	22	-2.4622523746689176
1115	23	-2.902744259030726
1115	24	-2.766830398748212
1115	25	-2.901822662695963
1116	1	-1.2674543072880233
1116	2	-1.1796203500549325
1116	3	-1.701109346163129
1116	4	-1.233452187535569
1116	5	-1.3988832159253803
1116	6	-3.8070959747202693
1116	7	-2.1402234178466415
1116	8	-2.3397230019003707
1116	9	-3.332145020202951
1116	10	-2.730597067254841
1116	11	-2.107056936517097
1116	12	-1.9328305347445287
1116	13	-3.2420223321276787
1116	14	-6.381966967281912
1116	15	-4.101336235357305
1116	16	-2.992185090347732
1116	17	-4.085754251730812
1116	18	-2.7609051554110238
1116	19	-2.411897148699264
1116	20	-3.022986462131726
1116	21	-3.7216918316044634
1116	22	-3.530288555402393
1116	23	-2.470566865982388
1116	24	-3.6017218056571565
1116	25	-3.525289791104697
1201	1	0.5767006013170253
1201	2	0.6546155302100338
1201	3	1.074057466724728
1201	4	0.8434680722820431
1201	5	0.4338253907342562
1201	6	0.9456281662637878
1201	7	1.0355977683962223
1201	8	1.2018478161065644
1201	9	1.5302361583130022
1201	10	1.3452530949299444
1201	11	1.6091285417066743
1201	12	1.3854126377991562
1201	13	1.456567907809216
1201	14	1.6101499667414814
1201	15	1.6104408752583552
1201	16	1.5207840583229952
1201	17	1.7213046096756486
1201	18	1.5380915526000223
1201	19	1.7372919273706344
1201	20	1.613285782940352
1201	21	1.8066623348945186
1201	22	1.5995647136472755
1201	23	1.9741591101446545
1201	24	1.9019351996792437
1201	25	2.0131311005494226
1202	1	1.149549700031148
1202	2	1.0296031988806433
1202	3	1.132831919791606
1202	4	1.350290431139193
1202	5	1.1632700006816918
1202	6	1.3765380474434892
1202	7	1.4304578424004788
1202	8	1.3863819685983074
1202	9	1.6931311474793347
1202	10	1.5225307280960934
1202	11	1.4407283261729447
1202	12	1.6293590538997194
1202	13	1.6313436241251509
1202	14	2.069034302941539
1202	15	1.7064867103620287
1202	16	1.415413947511965
1202	17	1.8524192804576387
1202	18	1.855865271484781
1202	19	1.882379011788558
1202	20	1.554291726396933
1202	21	1.7815894457495105
1202	22	1.7755431212046275
1202	23	1.919374147366554
1202	24	1.9389846991819226
1202	25	2.267981342048131
1203	1	0.9412983890440074
1203	2	0.9094450968785424
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2211	16	-0.38684036796573906
2211	17	0.06147322773015418
2211	18	-0.22838459251389764
2211	19	-0.03972203610949521
2211	20	-0.08322407606397775
2211	21	0.14224758113876845
2211	22	0.19050910759919049
2211	23	0.2743672905828234
2211	24	-0.09050598732186899
2211	25	0.3238333571605736
2212	1	0.1305467551631807
2212	2	0.23761148162703805
2212	3	-0.1356591324655554
2212	4	0.17059357835491795
2212	5	-0.07548635015643157
2212	6	-0.058015558432572334
2212	7	0.029524893902298288
2212	8	0.16419599424421705
2212	9	0.20877866843445503
2212	10	-0.11628536660851552
2212	11	-0.26050708582369
2212	12	0.1927000871918665
2212	13	0.24037357582541574
2212	14	0.2947653513568653
2212	15	-0.19198827453922007
2212	16	-0.09986371495635638
2212	17	-0.02808810287496044
2212	18	0.26926564166884503
2212	19	-0.028699975463375438
2212	20	0.12300238213063608
2212	21	0.2783222539228589
2212	22	0.3829655233638718
2212	23	0.4887340089300807
2212	24	0.45740078672378104
2212	25	0.46333352969922004
2213	1	-0.36029546878808105
2213	2	-0.22197291220645354
2213	3	-0.5001044634075456
2213	4	-0.1927347656823244
2213	5	-0.22575504682920666
2213	6	-0.05217841169451276
2213	7	-0.30637957536407967
2213	8	-0.09538609176509283
2213	9	-0.6703932183107213
2213	10	-0.26862055499290705
2213	11	-0.277191665601876
2213	12	-0.29509910572904374
2213	13	-0.39494437640922087
2213	14	-0.6470936339917515
2213	15	-0.4038129062764142
2213	16	-0.40011131152230917
2213	17	-0.43905059743509156
2213	18	-0.4820570712773886
2213	19	-0.5656310968021785
2213	20	-0.3142373282704014
2213	21	-0.4212072796329025
2213	22	-0.6073287571884194
2213	23	-0.311419955911866
2213	24	-0.13377541235573176
2213	25	0.02986381494628887
2214	1	-2.7284060250693223
2214	2	-1.4438715602220178
2214	3	-1.5642982331458022
2214	4	-3.272524858111101
2214	5	-1.76500627452808
2214	6	-1.4950587227666787
2214	7	-1.2807852919488987
2214	8	-1.2457798923680556
2214	9	-1.0371148627382638
2214	10	-1.0599744409147434
2214	11	-1.1238016459256244
2214	12	-1.1864904702247117
2214	13	-1.4515570022793582
2214	14	-1.3100751372132535
2214	15	-1.698924983746835
2214	16	-1.4839964892975175
2214	17	-3.6814701425113405
2214	18	-3.3076122226522457
2214	19	-2.102017004858414
2214	20	-1.7955145760203095
2214	21	-1.8609308475931314
2214	22	-2.7692961342945246
2214	23	-1.678261476660115
2214	24	-2.8263932356222057
2214	25	-2.7471986089253555
2215	1	-1.860937901714717
2215	2	-3.493369655152229
2215	3	-2.0482546481177444
2215	4	-3.4105387613025826
2215	5	-2.2568423778664304
2215	6	-2.1172049539113402
2215	7	-2.3250017155844667
2215	8	-1.7862115107460745
2215	9	-4.177627586634809
2215	10	-2.296721199726985
2215	11	-2.0428097750321896
2215	12	-1.8844357495124378
2215	13	-1.934258832987556
2215	14	-1.69352654276868
2215	15	-1.819899591617883
2215	16	-1.8737506107638282
2215	17	-2.2083635977634337
2215	18	-1.94946695207096
2215	19	-1.9139678120242465
2215	20	-1.8444981344009364
2215	21	-2.578350907096681
2215	22	-2.6211952451668026
2215	23	-2.453563865008892
2215	24	-2.3572026280194827
2215	25	-3.16104317464152
2216	1	-2.6849772752901195
2216	2	-2.755976572662604
2216	3	-4.188376578855777
2216	4	-4.664990731425746
2216	5	-4.273986634150091
2216	6	-3.226494557244962
2216	7	-5.159267396769515
2216	8	-2.718365647678983
2216	9	-3.3847462761533116
2216	10	-2.5673864396646913
2216	11	-2.775588965880832
2216	12	-3.0518943756011367
2216	13	-2.5628970366638057
2216	14	-3.60629412666853
2216	15	-2.8794225591716227
2216	16	-3.1746345598040193
2216	17	-2.8224174092993373
2216	18	-2.512897452924946
2216	19	-3.2581385477306775
2216	20	-3.8072168380246936
2216	21	-3.5273907040288677
2216	22	-3.0739294028073374
2216	23	-3.067844795007197
2216	24	-3.8486875783333367
2216	25	-3.8283990993553374
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84070.0
3	2.0
4	754.0
5	4613.0
6	6427.0
7	5854.0
8	5496.0
9	4809.0
10	4161.0
11	3878.0
12	3428.0
13	3331.0
14	3225.0
15	3244.0
16	3437.0
17	3638.0
18	4019.0
19	5034.0
20	6443.0
21	8481.0
22	10610.0
23	12878.0
24	14885.0
25	16736.0
26	18522.0
27	19963.0
28	22280.0
29	26176.0
30	31644.0
31	38654.0
32	48104.0
33	60581.0
34	78278.0
35	101142.0
36	137696.0
37	211451.0
38	575422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.709045774032106	19.594705079065843	13.188689168039305	23.50755997886274
2	13.92775205544446	20.603588989135375	43.67805315147175	21.790605803948417
3	18.620572485700443	26.77688914617955	29.597410033916223	25.005128334203786
4	11.480603784602838	17.152599837903416	39.683962002949954	31.682834374543788
5	11.832311093240001	39.9135516503404	35.717049983104246	12.537087273315347
6	29.81265195934275	37.94429117755778	19.222018542739423	13.021038320360049
7	27.16469809620545	32.917642450846614	22.493213327884266	17.424446125063678
8	24.33699499880324	37.98818357037578	21.29520918103828	16.379612249782692
9	27.27497717182531	14.937372083503888	21.26937246134954	36.518278283321266
10	15.570828565328645	29.615874637321998	34.35293584181288	20.46036095553648
11	32.70718573018261	23.156650467186132	26.154866362157573	17.98129744047368
12	23.893210591520056	25.74132893458116	32.18602993091364	18.17943054298514
13	28.810819422919607	23.639653164848713	26.31856364049702	21.23096377173466
14	19.95827201031801	24.647727222632618	28.679018806564383	26.71498196048499
15	21.935706112434637	33.96465958447712	24.093236024074155	20.006398279014086
16	19.8251279674338	29.37287140784329	30.44377997662327	20.358220648099635
17	19.736408670485645	30.636366785602938	29.155783420514776	20.471441123396644
18	19.57548818732486	27.732151551435162	33.17418916782508	19.518171093414896
19	22.20071760887734	27.60268627820224	29.733784363381922	20.462811749538503
20	22.998473347827293	27.720325383489484	30.597409729762408	18.683791538920815
21	21.744034106115613	27.422803112120075	29.034657128373382	21.798505653390933
22	22.23319585499016	29.943425604228658	28.897158305010446	18.92622023577074
23	20.13362035846231	29.538185972633524	30.49606306155379	19.83213060735038
24	22.006779029094908	27.370739332350023	31.274223189015505	19.348258449539564
25	20.926241515299225	28.63127559265375	30.778948747306117	19.6635341447409
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	317.0
1	317.0
2	792.0
3	1267.0
4	1267.0
5	1267.0
6	2508.0
7	3749.0
8	3749.0
9	3749.0
10	4655.0
11	5561.0
12	5561.0
13	5561.0
14	7472.5
15	9384.0
16	9384.0
17	9384.0
18	16530.0
19	23676.0
20	23676.0
21	23676.0
22	37531.5
23	51387.0
24	51387.0
25	51387.0
26	75218.0
27	99049.0
28	99049.0
29	99049.0
30	126300.0
31	153551.0
32	153551.0
33	153551.0
34	168465.5
35	183380.0
36	183380.0
37	183380.0
38	187886.5
39	192393.0
40	192393.0
41	192393.0
42	196902.5
43	201412.0
44	201412.0
45	201412.0
46	205830.5
47	210249.0
48	210249.0
49	210249.0
50	193415.5
51	176582.0
52	176582.0
53	176582.0
54	147048.5
55	117515.0
56	117515.0
57	117515.0
58	96659.5
59	75804.0
60	75804.0
61	75804.0
62	60909.0
63	46014.0
64	46014.0
65	46014.0
66	33976.5
67	21939.0
68	21939.0
69	21939.0
70	16120.0
71	10301.0
72	10301.0
73	10301.0
74	7108.5
75	3916.0
76	3916.0
77	3916.0
78	2643.0
79	1370.0
80	1370.0
81	1370.0
82	887.0
83	404.0
84	404.0
85	404.0
86	251.0
87	98.0
88	98.0
89	98.0
90	68.0
91	38.0
92	38.0
93	38.0
94	21.5
95	5.0
96	5.0
97	5.0
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.341834416993946
2	0.010821925220496726
3	0.009815234502310985
4	0.011954452278455686
5	0.013149897506301255
6	0.08550579287590146
7	0.08349241143952998
8	0.10985512462201909
9	0.08909212855943816
10	0.09595020907707853
11	0.09154593718501591
12	0.10337455312369838
13	0.08852586503045869
14	0.09204928254410878
15	0.09053924646683016
16	0.09198636437422218
17	0.10576544357938952
18	0.10740131599644134
19	0.10022864462936794
20	0.0992848720810688
21	0.08688999261340685
22	0.10834508854474048
23	0.09966238110038846
24	0.09613896358673836
25	0.09488060018900618
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1589366.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.90530753679969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45400962017193	47.59716881328241
2	12.637206272182366	15.140714562821172
3	3.9062237071941834	7.020105974610161
4	1.5869846873739952	3.802752230133243
5	0.756458465771124	2.2657938515417424
6	0.45005303433954874	1.6176339257988324
7	0.261773064608713	1.0977117158164842
8	0.1701693980084173	0.8155240096837046
9	0.11668766960170557	0.629118965991838
>10	0.5676323761046307	6.0909153951602635
>50	0.046103694716430346	1.9557819960162457
>100	0.0384574335916411	4.724997148091352
>500	0.005176772313238404	2.1695471821548065
>1k	0.0024299135347853732	2.555356972773701
>5k	5.282420727794289E-4	1.8543679240704734
>10k+	1.0564841455588578E-4	0.6625093320535739
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10468	0.6586274023730218	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6281	0.39518902505779036	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6133	0.38587713591457223	No Hit
GTCCTACAGTGGACATTTCTAAATT	6065	0.3815987003622828	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5569	0.3503912880985248	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5252	0.3304462282444698	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4517	0.2842013733778123	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2959	0.18617486469447567	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2587	0.16276930549665716	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2316	0.14571848145738614	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2275	0.1431388364920352	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2172	0.13665826499371447	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2055	0.12929683911698123	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1937	0.1218724950703614	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1719	0.10815633403508065	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1719	0.10815633403508065	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1633	0.10274537142483228	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.2583633977321775E-4
13	0.0	0.0	0.0	0.0	1.8875450965982662E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTT	25	0.0060380287	18.996788	7
GGACCGT	40	0.005285095	14.247591	6
TAGGACC	765	0.0	13.657392	4
AGGACCG	50	0.0015018408	13.297333	5
CCCGTAC	185	0.0	12.836476	16
GGTATCA	1830	0.0	12.70454	1
GGCGAGG	1125	0.0	12.495271	19
CCGTACA	195	0.0	12.178196	17
AGGACGA	135	3.7289283E-10	11.960564	5
CCCCGTA	200	0.0	11.873365	15
ACCGTGC	60	0.005883152	11.08146	8
TGTAGGA	2675	0.0	11.078152	2
TTAGGAC	1700	0.0	10.894873	3
GTATTAG	105	3.3439574E-6	10.8896055	1
TTAGACC	70	0.001493931	10.854964	4
TCAGTAC	70	0.001493931	10.854964	3
AGGACCT	1630	0.0	10.838317	5
GGACGAG	150	2.1373125E-9	10.764846	6
GTATCAA	4095	0.0	10.7267275	1
GGACCTG	1535	0.0	10.7050295	6
>>END_MODULE